BASIC TECHNIQUES IN BIOCHEMISTRY LAB.
(BIOTECHNOLOGY)
EXPERIMENTS
1.Pipetting and preparing solutions
2. Distruption and homogenization of biological samples
3. Concentration of extracts: Precipitation techniques
4. Membrane processes; dialysis, microfiltration, ultrafiltration
5. Spectrophotometry
a) BSA standard graph using Bradford method
b) Determination of protein content of plasma samples using Bradford
method
6. Chromatography
a) Isolation of plant pigments and preparation of column
b) Application of samples to column and scanning of absorbtion spectrums
of separated pigments
7. Electrophoresis: SDS Polyacrylamide gel electrophoresis of proteins
DATE
09.10.2014
16.10.2014
23.10.2014
30.10.2014
FINAL EXAM
12.12.2014
Lecturer
Emre ERDEN
ÇAKMAKLI
04-10-108
04-11-118
04-10-748
04-10-107
04-11-798
M. Yılmaz
O. Durmuş
Res.Asst.
Güliz AK
04-11-132
04-10-121
04-10-94
04-09-75
04-10-104
04-11-120
04-11-130
04-11-116
06.11.2014
13.11.2014
20.11.2014
27.11.2014
04.12.2014
Res.Asst.
Umut
MENGÜLLÜOĞLU
04-10-747
04-11-80
04-11-97
04-10-113
04-10-126
04-10-115
04-11-127
04-11-98
Attention: Each group will support their own biological material.
For distruption and homogenization experiments: green-leaf vegetables (spinach or
lettuce), friut (apple), cereal plant (rice and lentil), animal tissue (liver or heart),
For chromatography experiments: Spinach
For Precipitation techniques experiments: Blood tubes with EDTA
For : SDS Polyacrylamide gel electrophoresis: Plastic box (all groups).
Lect. Emre ERDEN ÇAKMAKLI
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