ZBORNIK APSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA
DRUŠTVA GENETIČARA SRBIJE
BOOK OF ABSTRACTS
IV SYMPOSIUM OF THE SECTION OF THE BREEDING OF ORGANISMS
OF THE SERBIAN GENETIC SOCIETY
OKRUGLI STO / ROUND TABLE
KVALITET HRANE - DOPRINOS NAUKE
FOOD QUALITY - CONTRIBUTION OF SCIENCE
Akademija inženjerskih
nauka Srbije
Beograd/Belgrade
2011
Izdava~/Publisher
Dru{tvo geneti~ara Srbije, Beograd
Serbian Genetic Society, Beograde
Urednici/Editors
Dr Jano{ Berenji
Dr Sne`ana Mladenovi} Drini}
Dr Kosana Konstantinov
Kompjuterski prelom/Computer prepress
[tamparija Feljton, Novi Sad
[tampa/Printing
Tira`/Number of copies printed
200
CIP - Katalogizacija u publikaciji
Biblioteka Matice srpske, Novi Sad
631.527(082)(048.3)
575(082)(048.3)
DRU[TVO geneti~ara Srbije. Sekcija za oplemewivawe
organizama. Simpozijum (4 ; 2011 ; Kladovo)
Zbornik apstrakata IV simpozijuma Sekcije za oplemenjivanje
organizama Dru{tva geneti~ara Srbije, Okrugli sto: Kvalitet hrane - doprinos
nauke = Book of abstracts of the IV Symposium of the Section of the breeding
of organisms of the Serbian genetic society, Round table: Food quality contribution of science, Kladovo, 2-6. oktobar 2011. / [urednik Jano{ Berenji].
- Beograd = Belgrade : Dru{tvo geneti~ara Srbije, 2011 (Novi Sad : Feljton). 160 str. ; 24 cm
Uporedo srp. tekst i engl. prevod. - Tira` 200. - Registar.
ISBN 978-86-87109-06-03
a) Poqoprivredne biqke / Oplemewivawe - Zbornici - Apstrakti
b) Genetika - Zbornici - Apstrakti
COBISS.SR-ID 266439175
Beograd, 2011
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA
DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011
IV SYMPOSIUM OF THE SECTION OF THE BREEDING OF ORGANISMS
OF THE SERBIAN GENETIC SOCIETY
Kladovo (Serbia), October 2-6, 2011
OKRUGLI STO
KVALITET HRANE - DOPRINOS NAUKE
ROUND TABLE
FOOD QUALITY - CONTRIBUTION OF SCIENCE
Organizacioni odbor/Organizing Committee
Milosav BABI]
Jan BO]ANSKI
Jelena BO[KOVI]
Sne`ana MLADENOVI] DRINI] - Presedednik
Borislav KOBILJSKI
Zoran LUGI]
Gordana [URLAN
MOMIROVI]
Dragoja RADANOVI]
Sa{a ORLOVI]
Svetlana PAUNOVI]
Milan ZDRAVKOVI]
Programski oodbor/Organizing Committee
Violeta AN\ELKOVI]
Jano{ BERENJI - Predsednik
Slobodan [email protected]]
Vladislava GALOVI]
Vasilije ISAJEV
Ana MARJANOVI] JEROMELA
Desimir [email protected]]
Kosana KONSTANTINOV
Sla|ana MARI]
Nenad PAVLOVI]
Jasmina RADOVI]
Ivica STAN^I]
Veselinka ZE^EVI]
Sekretarijat/Secretariat
Vladan IVETI]
Ivana LEHOCKI
Ministarstvo prosvete i nauke Republike Srbije
je podr`alo odr`avanje skupa odnosno {tampanje zbornika radova sa skupa
Uvodna izlaganja
Introductory lectures
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PRIMENA METODA BIOTEHNOLOGIJE U OPLEMENJIVANJU
BILJAKA U ARI HAS, MARTONVA[AR, MA\ARSKA
Csaba L. Marton, László Sági, Mariann Rakszegi, Tamás Spitkó, Klára Mészáros,
Boglárka Németh-Kisgyörgy, Alexandra Soltész, Fruzsina Szira, Helga Ambrus,
Annamária Mészáros, Gábor Galiba, Attila Vágújfalvi, Beáta Barnabás
Agricultural Research Institute of the Hungarian Academy Of Sciences (HAS),
Martonvásár, Hungary
Jedan od osnovnih nau~nih zadataka Poljoprivrednog Instituta Ma|arske akademije
nauka je ispitivanje svojstava p{enice i kukuruza i otkrivanje njihove biohemijske i
geneti~ke osnove. Ste~ena znanja se zatim primenjuju u oplemenjivanju biljaka.
Poslednjih deset godina, pored konvencionalnih selekcionih metoda, zna~ajnu ulogu
su imale moderne biotehnlolo{ke metode (npr. geneti~ke modifikacije ili genske
transformacije) koje, pored ostalog, omogu}avaju analizu pojedinih gena. Razvoj
geneti~ki modifikovanih biljaka zavisi od dostopnosti pouzdanih tehnika za transfer
gena prilago|enjih lokalnim laboratoriskim uslovima. Eksperimenti sa reporter
genima (npr. gusA i gfp) ili bar selektivnim marker genom, omogu~avaju pra}enje
uspe{nosti geneti~kih transfor macija jednopstavnom bojenom reakcijom ili
prisustvom rezistentosti na herbicide. “Korisni” geni mogu biti preneti samo nakon
optimizacije metodologije, koja se mora izvesti pojedina~no za svaku biljnu vrstu
namenjenu za geneti~ku modifikaciju (u na{em slu~aju p{enica, je~am i kukuruz). U
Martonvásár-u cilj geneti~kih modifikaciija je pobolj{anje agronomskih svojstava i
otpornosti na uslove spolja{nje sredine ovih biljaka, i delimi~no modifikacija kvaliteta
hleba i hranjivih vrednosti koji zadovoljavaja zahteve prera|iva~ke industrije.
APPLICATION OF BIOTECHNOLOGICAL METHODS IN PLANT
BREEDING IN ARI OF HAS, MARTONVASAR, HUNGARY
One of the basic scientific tasks of the Agricultural Research Institute of the Hungarian Academy of Sciences is to investigate the characteristics of wheat and maize and
to reveal their biochemical and genetic backgrounds. The knowledge acquired is then
exploited in plant breeding. In addition to conventional breeding methods, an increasing role has been played over the last ten years by modern biotechnological proce dures (e.g. ge netic mod i fi ca tion or gene trans for ma tion) which, among other
things, allows the effects of individual genes to be analysed. The development of genetically modified plants is dependent on the availability of reliable gene transfer
techniques adapted to local laboratory conditions. This purpose is served by experiments involving reporter genes (e.g. gusA and gfp) or the bar selectable marker gene,
where the success of gene transfer is indicated by a simple colour reaction or by the
presence of herbicide resistance. “Useful” genes can only be transferred after the optimisation of the methodology, which must be carried out individually for each plant
species intended for genetic modification (in the present case wheat, barley and
maize). In Martonvásár the aim of genetic modification is partly to improve the agronomic traits and environmental resistance of these plants, and partly to mod ify
breadmaking quality or nutritional value to satisfy the requirements of the processing
industry.
6
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
SADA[NJOST I BUDU]NOST OPLEMENJIVANJA
I SEMENARSTVA U SRBIJI?
Borislav Kobiljski i Ankica Kondi}-[pika
Institut za ratarstvo i povrtarstvo, Novi Sad
Oplemenjivanje biljaka je umetnost, ali i nau~no-zasnovana tehnologija koja
uklju~uje razli~ite metode u stvaranje komercijalnih sorti gajenih biljaka
unapre|enog kvaliteta i prinosa, kao odgovor na izazov rastu}ih globalnih potreba za
hranom. Integracijom nedavnih dostignu}a u biotehnologiji, genomici i primeni
molekularnih markera sa konvencionalnim oplemenjivanjem, stvorena je osnova za
marker asistiranu selekciju (MAS) i molekularno oplemenjivanje biljaka,
interdisciplinarne pristupe koji mogu dovesti do revolucionarnog unapre|enja
gajenih biljaka u 21. veku. Dostupnost markera za va{na agronomska svojstva, u
kombinaciji sa efikasnijim sistemima njihove detekcije, doprine}e ostvarenju punog
doprinosa MAS u nastojanjima oplemenjivanja da se unesu i piramidiraju geni od
interesa u superiornim genotipovima, u cilju pove}anja proizvodnje gajenih biljaka
{irom sveta. Pored toga, markeri se mogu koristiti i u semenarstvu za preciznu
identifikaciju geneti~kog materijala (genoti pi zaciju) koji je za{ti}en pravom
intelektualne svojine. U ovom radu bi}e duskutovano sada{nje stanje i perspektive
budu}e primene najnovijih znanja i tehnologija u oplemenjivanju biljaka i
semenarstvu u Srbiji.
PRESENT AND FUTURE OF PLANT BREEDING
AND SEED PRODUCTION IN SERBIA?
Plant breeding is an art and science-based technology that incorporate different
methods in development of commercial cultivars of crop plants with improved quality
and yield to meet the increasing challenges of global food demand. Integration of recent advances in biotechnology, genomic research and molecular marker applications
with conventional plant breeding has created the foundation for marker-assisted selection (MAS) and molecular plant breeding, interdisciplinary approaches that could
revolutionize crop improvement in the 21st century. The availability of molecular
markers for important agronomic traits combined with more efficient marker detection systems will help reach the full benefit of MAS in the breeding efforts for
introgression and pyramiding genes of interest in superior genotypes to enhance crop
production worldwide. Also, molecular markers could be used in seed industry for
precise identification of genetic material (genotyping) that is protected with intellectual property rights. This report discusses present status and future prospects of application of the latest knowledge and technologies in plant breeding and seed industry in
Serbia.
7
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ULOGA BILJNE SORTE U ORGANSKOJ POLJOPRIVREDI
Jano{ Berenji i Vladimir Sikora
Institut za ratarstvo i povrtarstvo, Novi Sad
Organska proizvodnja te{i potpunom osmostaljenju od konvencionalne proizvodnje u
svakom pogledu, podrazumevaju}i i biljnu sortu. Zbog interakcije genotip x spoljna
sredina u uslovima organske proizvodnje konvencionalne sorte nisu uvek najefikasnije.
Cilj oganskog oplemenjivanja biljaka je stvaranje organskih sorti koje su posebno
prilago|ene zahtevima i uslovima organske poljoprivrede. U organskom oplemenjivanju koriste se samo uslovno dozvoljene (somatska embriogeneza, kultura embrija, kultura antere...) i dozvoljene metode i tehnike (selekcija, molekularni markeri...) oplemenjivana. Od ciljeva oplemenjivanja posebna pa{nja se poklanja kvalitetu i
funkcionalnoj divergentnosti ploda kao i otpornosti na bioti~ke i abioti~ke stresove.
Mesto i uloga hibridnih sorti u organskom oplemenjivanju i proizvodnji nije do kraja
razja{njena. U organskom oplemenjivanju biljni geneti~ki resursi dolaze do posebnog
izra{aja. Autohtone sorte koje su prilago|ene niskim imputima (low input) su od
posebnog interesa za organsku poljoprivredu. U na{im zakonskim propisima pojam
autohtone sorte nije definisan.
Krajnji cilj organske poljoprivrede je kori{}enje organskog semena. Ukoliko takvo
seme nije dostupno u bazi reproduktivnog materijala, odobrava se i seme koje poti~e
iz perioda konverzije ili iz konvencionalne proizvodnje, pod uslovim da nije tretirano
sredstvima koja nisu dozvoljena u organskoj proizvodnji. Postoji velika potreba za
pronala{enjem efektivnih alternativnih na~ina tretiranja semena. Status semena koje
bi organski proizvo|a~i proizvodili za sopstvene potrebe nije do kraja razja{njen.
Klju~ne re~i: autohtona sorta, organska poljoprivreda, organsko oplemenjivanje,
organska sorta
THE ROLE OF PLANT VARIETY IN ORGANIC AGRICULTURE
Organic farming seeks full independence from conventional production in every respect, including the plant variety. Because of the genotype x environment interaction
conventional varieties are not always the most effective in organic production. The
purpose of organic plant breeding is to create organic varieties that are specially
adapted to the requirements and conditions of organic agriculture. In organic breeding only conditionally permitted (somatic embryogenesis, embryo culture, anther culture...) and permitted breeding methods and techniques (selection, molecular markers...) are allowed. Among breeding objectives, special attention is paid to quality and
functional diversity of the fruit as well as to resistance to biotic and abiotic stresses.
The place and role of hybrid varieties in organic production has not been fully clarified. Genetic resources are of particular importance in organic agriculture. Landraces
and old varieties that are well adapted to low inputs are of particular interest for organic farming. In our legislative the terms landraces and old varieties are not defined.
The ultimate aim of organic agriculture is to use organic seed. If this seed is not available from the database of reproductive material, seed derived from the conversion
period or from conventional production are legal, provided it is not treated in the way
not allowed in organic production. There is an urgent need to develop more effective
alternative seed treatments. The status of the seed produced by organic farmers for
their own use is not completely understood.
Key words: landraces and old varieties, organic agriculture, organic breeding, organic
variety
8
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NOVI PRISTUPI U PROGRAMIMA
OPLEMENJIVANJA [UMSKOG DRVE]A
V. Isajev1, S. Orlovi}2, M. Mataruga3, K. Konstantinov4 i S. Mladenovi}-Drini}4
1[umarski fakultet, Beograd
2Institut za nizijsko {umarstvo i za{titu {ivotne sredine, Novi
3[umarski fakultet, Banja Luka, Republika Srpska
4Institut za kukuruz ”Zemun Polje”, Beograd
Sad
Programi savremenog oplemenjivanja {umskih vrsta drve}a obuhvataju odr{ivo i
usmereno kori{}enje genetske varijabilnosti stabala u cilju identifikacije i
umno{avanja superiornih genotipova zna~ajnih za {umarsku struku. U prirodnim
popumacijama vrsta drve}a, koje se gaje za potrebe industrijske prerade, i koje se
geneti~ki me|usobom razlikuju, najvi{e se primenjuju metodi vi{estruke selekcije.
Genetska dobit se ostvaruje u semenskim planta{ama pri slobodnom opra{ivanju dok
se zna~ajni razultati posti{u primenom unapre|enih tehnika osnivananja i kori{}enja
klonskih semenskih planta{a i semenskih planta{a od linija punih srodnika,
superiornih genotipova, sprovo|enjem kontrolisane hibridizacije.
Primena savremenih metoda biotehnologije, kao {to su molekularni markeri,
somaklo nalna varijabilnost, geneti~ko in{enjerstvo, fuzija protoplasta,
mikropropagacija i druge, sve vi{e se primenjuju u programima oplemenjivanja
drve}e, kako za potrebe industrije prerade, tako i u druge svrhe. U odnosu na
specfi~nosti vrsta, i prema savremenim zahtevima tr{i{ta, primene navedenih metoda
rada se zna~ajno razlikuju.
Neki od postignutih rezultata u oplemenjivanju drve}a, kod vrsta iz rodova Fagus,
Pinus, Picea i Populus, primenom navedenih metoda biotehnike, prikazani su u radu.
Klju~ne re~i: genetika, oplemenjivanje, {umsko drve}e, biotehnologija
THE NEW APPROUCHES IN GENETIC IMPROVEMENT
PROGRAMME OF FOREST TREES
The general objective of a genetic improvement programme of forest trees should be
the sustainable management of genetic variation in order to produce, identify and
multiply well-adapted genotypes for operational planning. For industrial forestry species, the most common approach is recurrent selection in genetically diverse breeding
populations. Genetic gains are most commonly captured in open-pollinated general
combiner seed orchards, while a few advanced programmes feature deployment of superior full-sib families or clones.
Various biotechniques, such as: molecular markers, somaclonal variation, genetic engineering, protoplast fusion, micropropagation and others, have a potential to genetically improve both industrial and non-industrial trees. The actual demand and applicability of these techniques to the various tree species differ greatly.
Some of the derived results of applied biotechniques for improvement of some species of the genus Fagus, Pinus, Picea and Populus, are presented in this paper.
Key words: genetic, improvement, forest trees, biotechnology
9
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
BIODIVERZITET - ZNA^AJ I KORI[]ENJE
Violeta An|elkovi}, Dragana Ignjatovi}-Mici},
Jelena Van~etovi} i Sne{ana Mladenovi} Drini}
Institut za kukuruz Zemun Polje, Beograd
Zna~aj biolo{kog diverziteta je nemerljiv za ~ove~anstvo. Oko 40% svetske
ekonomije zasnovano je na biolo{kim proizvodima i procesima. Efikasno kori{}enje
biodiverziteta na nivou gena, vrste i ekosistema je preduslov odr{ivog razvoja.
Me|utim, aktivnost ljudi izaziva stalno pove}anje gubitka vrste. Varijabilnost gena
unutar vrste predstavlja geneti~ki diverzitet, koji u oplemenjivanju biljaka
omogu}ava razvoj novih i pobolj{anih varijeteta sa po{eljnim karakteristikama.
Geneti~ka varijabilnost, prisutna u divljim srodnicima, koji su adaptirani na
specifi~ne uslove sredine ili poseduju specifi~na svojstva (otpornost na bolesti,
insekte ili abioti~ki stres) i tradicionalno gajenim sortama, je vrlo zna~ajna u
proizvodnji hrane i u ljudskoj ishrani. Imaju}i u vidu geneti~ku varijabilnost, kukuruz
je jedna od najbolje o~uvanih vrsta (podaci FAO). Banka gena Instituta za kukuruz,
sa vi{e od 6000 uzoraka, jedna je od najve}ih u svetu. Njeni najva{niji zadaci su
konzervacija i iskori{}avanje tih genotipova, a u radu }e biti prikazani neki od
rezultata pribriding aktivnosti.
Klju~ne re~i: banka gena, diverzitet, kukuruz, oplemenjivanje
BIODIVERSITY - IMPORTANCE AND EXPLOITATION
The importance of biological diversity to human society is difficult to measure. An estimated 40% of the global economy is based on biological products and processes.
The effective use of biodiversity at all levels - genes, species and ecosystems - is therefore a precondition for sustainable development. However, human activities cause
the progressive loss of species. Variety of genes within a species is the genetic diversity. In plant breeding it makes possible development, through selection and breeding, of new and improved varieties that posses desired characteristics. Plant genetic
resources present in wild varieties, adapted to specific environments or displaying
specific traits (resistance to diseases, pests or environmental stresses) and traditionally grown landraces are very important in food production and nutrition. According
to FAO, maize is one of the most perserve specie, considering genetic variability.
MRI genebank with more than 6000 accessions is one of the largest collection in the
world. Conservation and utilisation of maize genotypes are the most imortant tasks
and some results of prebreeding activities will be presented.
Key words: breeding, diversity, genebank, maize
10
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POSTUPAK PRIZNAVANJA SORTI POLJOPRIVREDNOG
BILJA U REPUBLICI SRBIJI
Jan Bo}anski i Dragan Dedi}
Ministarstvo poljoprivrede, trgovine, {umarstva i vodoprivrede, Uprava za za{titu bilja, Beograd
Priznavanje sorti poljoprivrednog bilja je postupak ~ijim se sprovo|enjem omogu}ava
da se sorta prizna i nakon toga upi{e u Registar sorti poljoprivrednog bilja. Seme i
sadni materijal sorti upisanih u Registar mogu se proizvoditi i stavljati u promet na
teritoriji Republike Srbije. Najva{niji me|unarodni pravni izvori koji defini{u ovu
oblast su UPOV konvencija kao i veliki broj direktiva Evropske Unije. Dono{enjem
Zakona o priznavanju sorti poljoprivrednog bilja 2010. godine jasno su definisani svi
postupci potrebni da bi se sorta poljoprivrednog bilja priznala, odnosno upisala u
Registar sorti. U ovom radu je predstavljena u potpunosi zakonska regulativa i
neophodna procedura u postupku priznavanja sorti poljoprivrednog bilja. Tako|e su
prikazani neki statisti~ki podaci iz ove oblasti.
Klju~ne re~i: sorta poljoprivrednog bilja, UPOV, DUS, VCU, registar sorti
THE PROCEDURE OF AGRICULTURAL PLANT VARIETY
REGISTRATION IN REPUBLIC OF SERBIA
Registration of agricultural plant varieties is a procedure which implementation enables registration of plant varieties and enlisting in Register of Agricultural Plant Varieties afterwards. Seed and seedlings of varieties enlisted in Register can be produced and marketed in the territory of Republic of Serbia. UPOV Convention and
large number of EU directives are the most important legal sources which define this
issue. All procedures which are required for registration of an agricultural plant variety, ie. for enlisting in Register of Agricultural Plant Varieties, are clearly defined by
adopting Law on Registration of Agricultural Plant varieties in 2010. Complete legislation and necessary procedure in the process of registration of an agricultural plant
varieties are presented in this paper. There are also some statistical data from this
area.
Key words: agricultural plant variety, UPOV, DUS, VCU, Register of Agricultural
Plant Varieties
11
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NOVI TRENDOVI U BILJNOJ BIOTEHNOLOGIJI
Sne{ana Mladenovi} Drini}1, Domagoj [imi}2 i Aleksandra Sudari}2
1Institut za kukuruz “Zemun Polje”, Beograd
2Poljoprivredni institut, Osijek, Hrvatska
Dostignu}a u razli~itim nau~nim oblastima rezultirala su u razvoju novih pristupa i
tehnika u molekularnom oplemenjivanju, „omika“ tehnologijama i geneti~kim
transformacijama. Poslednjih godina je napravljen zna~ajan napredak u razvoju DNK
marker tehnologija {to je rezultiralo njihovim uklju~ivanjem u oplemenjiva~ke
programe za analize kao {to su izbor genotipova nosioca po{eljnih svojstava za
tolerantnost na stres i pobolj{an kvalitet, ranu selekciju, izbor roditelja u povratnim
ukr{tanjima. SNP markeri pru{aju ogroman potencijal za otkrivanje i detekciju va{nih
gena u biljkama. Tehnologije za sekvencioniranje genoma nove generacije, uz brze
sekvencioniranje ve}e propusne mo}i i manju cenu, se primenjuju kod ve}eg broja
biljnih vrsta. Omika tehnologije obuhvataju genomiku, proteomiku, metabolomiku,
interaktomiku, fenomiku. TILLING tehnologija koristi heteordupleksnu analizu za
detektovanje koji organizmi u populaciji su nosioci mutacija jednog nukleotida u
specifi~nim genima. Razvijene su nove tehnike kao sto su cink finger nukleaze
tehnologija, oligonukleotidna usmerena mutageneza, cisgeneza i intrageneza, RNK
zavisna DNK metilacija, reverzno opleme njivanje, agro infiltracija, sinteti~ka
genomika.
NEW TRENDS IN PLANT BIOTECNOLOGY
Achievements in various fields of science have resulted in the development of new
approaches and techniques in molecular breeding, „omics“ technologies and genetic
transformation. Recently, significant progress has been made in the development of
DNA marker technology, which resulted in their inclusion in breeding programs for
analysis, such as choice of carrier genotypes of desirable traits for tolerance to stress
and improved quality, early selection, the choice of donor parent in backcrossing.
SNP offer enormous potential in the discovery and detection of important genes in
crops. Next generation sequencing tehnologies, brought great improvments sequencing troughput and cost, are used in many plant species. „Omics“ technologies include
genomics, proteomics, metabolomics, interactomics, phenomics. Targeting induced
local lesions in genome TILLING is powerful technology that employes heteroduplex
analysis to detect which organisms in population carry single nucleotide mutations in
spe cific genes The new tech niques such as zinc finger nuclease tech nol ogy,
oligonukleotiddirected mutagenesis, cisgenezis and intragenezis, RNA dependent
DNA methylation, the reverse processing, agro-infiltration, synthetic genomics are
developed.
12
Sekcija / Section 1
Oplemenjivanje pravih
i prosolikih `ita
Breeding of cereals
and pseudocereals
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DOPRINOS OPLEMENJIVANJA POBOLJ[ANJU
TEHNOLO[KOG KVALITETA P[ENICE U SRBIJI
Novica Mladenov1 , Nikola Hristov1, Veselinka \uri}1, Bojan Jockovi}1,
Zoran Martinovi}2, Bogdan Garalei}3 i Sava Krgovi}3
1Institut za ratarstvo i povrtarstvo, Novi Sad
2Poljoprivredna stru~na slu`ba, Sremska Mitrovica
3Poljoprivredna stru~na slu`ba, Pan~evo
Sa ciljem da se oceni doprinos oplemenjivanja unapre|enju tehnolo{kog kvaliteta
p{enice u proteklih 50 godina, analizirano je 25 sorti p{enice, razli~itih godina priznavanja. Ogled je izveden na tri lokaliteta (Novi Sad, Sr. Mitrovica i Pan~evo) u 2009. i
2010. godini, po slu~ajnom blok sistemu u tri ponavljanja. Promene pokazatelja
kvaliteta (sadr`aj proteina, vla`an gluten, gluten indeks i vrednosni broj sredine
hleba) analizirane su primenom linearne regresije.
Izme|u analiziranih sorti, kod svih posmatranih parametara, dobijene su zna~ajne
razlike. Promene pokazatelja kvaliteta izra`ene su koeficijentom regresije i promene
geneti~kog potencijala po godini iznosile su -0,04% za sadr`aj proteina, -0,08% za
vla`an gluten, 0,86% za gluten indeks i 0,08 za vrednosni broj sredine hleba. Rezultati
ukazuju da je smanjenje sadr`aja proteina i vla`nog glutena, kompenzovano
pobolj{anjem kvaliteta proteina. Pove}anje stabilnosti glutenske strukture, a
verovatno i odgovaraju}a kombinacija gluteninskih subjedinica, doprineli su
pobolj{anju ostalih pokazatelja kvaliteta
Klju~ne re~i: sadr`aj proteina, vla`an gluten, gluten indeks, vrednosni broj sredine
hleba, regresija
THE CONTRIBUTION OF BREEDING IMPROVEMENT OF
TECHNOLOGICAL QUALITY OF WHEAT IN SERBIA
In order to evaluate the contribution of technological improvement of processing
quality of wheat in the past 50 years, 25 wheat varieties released in different periods
were analyzed. The experiment was conducted at three sites (Novi Sad, Sremska
Mitrovica and Pan~evo) in 2009 and 2010, according to a randomized block design
with three replications. Changes in indicators of quality (protein content, wet gluten,
gluten index and the value of bread crumb) were analyzed using linear regression.
Among the varieties analyzed, observed in all parameters, significant differences
were obtained. Changes in quality indicators are given by regression coefficient and
genetic potential for change were as follows: -0.04% for protein content, -0.08% for
the wet gluten, 0.86% for the gluten index and 0.08 for the values of bread crumb. The
results indicate that the reductions in protein content and wet gluten were compensated by improving the quality of protein. Increased stability of the gluten structure,
and probably an appropriate combination of glutenin subunits contributed to the improvement of other quality indicators
Key words: protein content, wet gluten, gluten index, values of bread crumb, regression
14
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DATUM KLASANJA I [email protected] VLAGE U ZRNU
P[ENICE KAO POKAZATELJI [email protected] VEGETACIJE
Nikola Hristov, Novica Mladenov, Bojan Jockovi},
Ankica Kondi}-[pika i Veselinka \uri}
Institut za ratarstvo i povrtarstvo, Novi Sad
U radu je ispitivana me|uzavisnost sadr`aja vlage u zrnu u zavr{nim fazama zrenja i
datuma klasanja kod 57 divergentnih genotipova p{enice. Datum klasanja izra`em je
brojem dana od 01. januara do datuma kada je vi{e od 50% klasova iza{lo iz lisnog
rukavca. Sadr`aj vlage u zrnu utvr|en je u tri razli~ita termina. Izme|u prose~nog
sadr`aja vlage u prvom terminu, koji se kretao od 12.1 do 32.1% (X=18.2%) i datuma
klasanja, koji se kretao od 131 do 138 dana, utvr|ena je visoko zna~ajna korelativna
veza (r=0.507**). Izme|u sadr`aja vlage u drugom terminu (11.3 - 29.5%, X=14.8%) i
datuma klasanja, utvr|ena je tako|e visoko zna~ajna korelacija (r=0.446**). Kada je
ve}ina genotipova u{la u punu zrelost, u tre}em terminu, sadr`aj vlage (11.6 - 19.3%,
X=13%) i datum klasanja nisu ispoljili korelativnu zavisnost (r=0.213). Visoko
zna~ajna korelacija izme|u datuma klasanja i sadr`aja vlage u vo{tanoj zrelosti, pored
du`ine vegetacije ukazuje i na veliku kompleksnost produ`enja perioda nalivanja
zrna kod ranostasnih genotipova.
Klju~ne re~i: datum klasanja, sadr`aj vlage, du`ina vegetacije, korelacije
HEADING DATE AND GRAIN MOISTURE CONTENT IN
WHEAT AS INDICATORS OF GROWING SEASON LENGTH
The paper examined the relationship between grain moisture content in the final
stages of maturation and heading date in 57 divergent genotypes of wheat. Heading
date was expressed as a number of days from 01 January to date when more than 50%
of spikes emerged from leaf sheet. The moisture content in grain was measured in
three different terms. Between the average moisture content in the first period, which
ranged from 12.1 to 32.1% (X = 18.2%) and heading date, which ranged from 131 to
138 days, was found highly significant correlation (r = 0,507 **). Between the moisture content in the second period (11.3 - 29.5%, X = 14.8%) and heading date, a
highly significant correlation was also established (r = 0,446 **). When the majority of
genotypes entered into full maturity in the third period, the moisture content (11.6 19.3%, X = 13%) and heading date showed no correlative dependence (r = 0213).
Highly significant correlation between heading date and the grain moisture content in
wax maturity, in addition to the growing season length indicates the great complexity
of extending the period of grain filling in early maturing genotypes.
Key words: heading date, moisture content, growing season length, correlations
15
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POVEZANOST PROTEINA KODIRANIH SA GLI- LOKUSA I KOMPONENTI
TEHNOLO[KOG KVALITETA KOD P[ENICE (Triticum aestivum L.)
Desimir Kne`evi}1, Aleksandra Yurievna Dragovi}2, Alexander Mihailovich
Kudryavtsev2, Veselinka Ze~evi}3, Jelena Bo{kovi}3 i Nevena \uki}4
2Institut
1Poljoprivredni fakultet, Le{ak
op{te genetike N.I. Vavilova Ruske Akademije Nauka, Moskva, Rusija
3Fakultet za Biofarming Ba~ka Topola
4Prirodno-matemati~ki fakultet, Kragujevac
Metodom elektroforeze analiziran je sastav glijadina kod 10 geneti~ki divergentnih
sorti p{enice. Komponente tehnolo{kog kvaliteta su analizirane kori{}enjem
standardnih laboratorijsih metoda. Izu~avana je veza izme|u glijadinskih proteina
koji su pod kontrolom Gli-1 lokusa i komponenti kvaliteta. Ustanovljeno je da sorte
koje imaju Gli-B1b, Gli-D1b, Glu-D1d imaju visoke vrednosti sedimentacije proteina i
volumena hleba. Tako|e je ustanovljena pozitivna korelacija izme|u Gli-D2a i
sedimentacije proteina, kao i izme|u Gli-B1l i volumena hleba. Identifikovani Gli-1
mogu se koristiti kao markeri za kvalitet u oplemenjivanju p{enice.
Klju~ne re~i: p{enica, glijadini, aleli, sedimentacija proteina, volumen hleba
RELATIONSHIP AMONG PROTEINS ENCODED BY GLI- LOCI AND
TECHNOLOGICAL QUALITY COMPONENTS IN WHEAT (Triticum aestivum L.)
The gliadin composition for 10 genetically divergent wheat cultivars were analyzed by
acid polyacrylamide gel electrophoresis method. Values of technological quality parameters were analyzed by using standard laboratory methods. The relationships between Gli-1, encoding gliadin proteins and quality components were analyzed. The
high sedimentation protein volume and high loaf volume values were established in
cultivars which carried Gli-B1b, Gli-D1b. Also, positive correlation between Gli-D2a
and sedimentation volume as well as between Gli-B1l and loaf volume were established in analyzed cultivars. The identified alleles at the Gli-1 loci can use as markers
of quality in wheat breeding.
Key words: wheat, gliadins, allele, protein sedimentation volume, loaf volume
16
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OPLEMENJIVANJE P[ENICE U USLOVIMA
ABIOTI^KOG STRESA SOLONJECA
Miodrag Dimitrijevi}, Sofija Petrovi} i Borislav Banjac
Poljoprivredni fakultet, Departman za ratarstvo i povrtarstvo, Novi Sad
Uslovi abioti~kog stresa su sve prisutniji u poljoprivrednojh proizvodnji, kao
posledica globalnih promena klime. Ove promene su dovele do ~e{}eg pojavljivanja
ekstremnih vrednosti tem per a ture i padavina i promena prose~nih vrednosti
meteorolo{kih parametara. Ponovlivost uslova godine je sve manja i manja.
Istovremeno se menjaju i drugi uslovi u poljoprivredi. Intenzifikacija proizvodnje je
dovela do opadanja kvalitetnih proizvodnih povr{ina. Humana populacija se ubrzano
uve}ava. Zahtevi za smanjenjem upotrebe ve{ta~kih |ubriva i za{titinih sredstava su
sve ve}i. Tra`e se putevi obezbe|ivanja hranom uz o~uvanje prirodne sredine.
Sve ove promene tra`e i druga~iji pristup oplemenjivanju p{enice. Iskustva koja se
sti~u ispitivanjima u uslovima abioti~kog stresa su veoma dragocena za postavljanje
novih selekcionih kriterijuma u oplemenjivanju.
Vi{egodi{nji eksperimenti i ukr{tanja p{enice u uslovima solonjeca ukazuju na
mogu}nost kreiranja po`eljne geneti~ke varijacije ukr{tanjem roditeljskih sorti iz
postoje}eg sortimenta p{enice i rekombinacijom roditeljskih genoma dobijanje
genotipova pobolj{ane tolerancije na abiotski stres.
WHEAT BREEDING IN STRESSFUL
CONDITIONS OF SOLONETZ SOILS
Global climatic changes lead to abiotic stress conditions as a growing reality in food
production. The changes are expressed through more frequent appearance of extreme temperature and rainfall, as well as, the differences in average values of meteorological parameters. Repetitiveness of weather conditions through years is getting
smaller. Moreover, intensification in agriculture lead to environmental damages.
Decrement of arable land quality is evident. Human population is growing rapidly.
Public awarnes of environmental preservation demand the diminishment of fertilizers
and other chemicals. Suficiant food production with environmental preservation is on
demand.
A new reqirements direct a different approach in wheat breeding. The experience obtained in abiotic stress conditions studies is valuable for establising a new selection
criteria in breeding.
Longlasting wheat field experiments of varieties and crosses in abiotic stress conditions of solonetz soil suggest the possibility of desirable genetic variability creation by
crossing and recombining the existing gene fond. Right selection criteria give a possibility of selecting genotypes with improved tolerance to abiotic stress.
17
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VARIJABILNOST [email protected] PRVE KLASE ZRNA KOD OZIMOG JE^MA
GAJENOG U USLOVIMA ISHRANE RAZLI^ITIM DOZAMA AZOTA
Desimir Kne`evi}1, Aleksandar Paunovi}2, Vlado Kova~evi}3,
Milomirka Madi}2 i Miroljub Aksi}1
1Poljoprivredni fakultet, Le{ak
2Agronomski fakultet, ^a~ak
3Poljoprivredni
fakultet, Osijek, Hrvatska
Izu~avana je varijabilnost sadr`aja zrna prve klase (debljina zrna ve}a od 2,5 mm) kod
genotipova ozimog je~ma (G-3003, G-3020, G-3007-1/02 i G-3019). Ovi genotipovi
je~ma su eksperimentalno izu~avani u toku dve sezone pri ishrani razli~itim dozama
azota (kontrolna = 0, N1=20, N2= 40 i N3=60 kg ha-1). Najve}i prose~an sadr`aj zrna
prve klase za sve ispitivane genotipove je na|en na varijanti ishrane N2=40kg ha-1 u
prvoj godini (81,8%) i u drugoj godini (83,2%). Pove}anje doze azota preko 40kg ha-1
uticalo je na smanjenje procentnog udela zrna prve klase. Dakle, na varijanti ishrane
N3=60kg ha-1, procentualni udeo prve klase zrna u proseku za sve genotipove bio je
smanjen i u prvoj (78,6%) i drugoj godini (82,9%). U proseku za sve varijante ishrane
azotom i sezone gajenja, genotip G-3019 je imao najve}u vrednost sadr`aja prve klase
zrna (82,9%), dok je~am G-3020 je imao najmanji procentni sadr`aj prve klase zrna
(78,7%). Analiza varijanse za prose~ni udeo prve klase zrna u interakciji genotip/doza
azota, ukazuju na postojanje zna~ajnih i visoko zna~ajnih razlika u obe
eksperimentalne godine.
Klju~ne re~i: je~am, genotip, zrna prve klase, azota ishrana
VARIABILITY OF FIRST-CLASS GRAIN CONTENTS OF WINTER BARLEY
GROWN UNDER THE DIFFERENT RATE OF NITROGEN NUTRITION
The variability of grain contents of first-class (thickness >2.5mm) in winter barley genotypes (G-3003, G-3020, G-3007-1/02 and G-3019) was studied. These barley genotypes are tested during two seasons by application of different rate of nitrogen nutrition (control=0, N1=20, N2=40 and N3=60kg ha-1). The highest average grain contents
of first-class for all tested genotypes was found on variant of nutrition by N2=40kg
ha-1 in the first year (81.8%) and in the second year (83.2%). The increasing N rates
over 40 kg ha-1 induced reduction in the first-class grain percentage. So, on variant of
nutrition by N3=60kg ha-1, the first-class grain percentage in average for all genotypes
reduced in first (78.6%) and in second year (82.9%). In average for all variant of N
nutrition and season, the genotype G-3019 had the highest value of first-class grain
contents (82.9%) while the barley G-3020 had the lowest value of first-class grain percentage (78.7%). Analysis of variance for average impact of first class grain in interaction genotype/nitrogen rate, indicated significnt and high significant differences in
both experimental years.
Key words: barley, genotype, first-class grain percentage, nitrogen nutrition
18
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VARIJABILNOST I ME\UZAVISNOST KOMPONENTI PRINOSA
PIVSKOG JE^MA GAJENOG NA ZEMLJI[TU TIPA PSEUDOGLEJ
Milomirka Madi}1, Aleksandar Paunovi}1, Nikola Bokan1,
Miodrag Jeli}2 i Desimir Kne`evi}2
1Agronomski fakultet, ^a~ak
2Poljoprivredni fakultet, Le{ak
Ve}i deo na{e zemlje, po koli~ini padavina spada u semiaridna podru~ja, koja su po
pravilu manje povoljna za proizvodnju dobrog pivskog je~ma. Zadnjih godina do{lo je
do smanjenja povr{ina pod je~mom (za oko 30%) koje nije pra}eno pove}anjem
prinosa zrna po jedinici povr{ine, jer se je~am gaji i na zemlji{tima slabijeg kvaliteta i
u manje povoljnim, semiaridnim podru~jima. ^etiri genotipa ozimog dvoredog je~ma
Rekord, Jagodinac, Kristal i Premijum su odabrana za prou~avanja varijabilnosti
komponenti prinosa (broja klasova po m2, visine stabla, du`ine klasa, broja i mase
zrna po klasu). Eksperiment je zasnovan na kiselom zemlji{tu (pHH2O 4.5) tipa
pseudoglej (2008-2011. godine) na imanju Srednje poljoprivredne {kole u Kraljevu, u
tri ponavljanja. Utvr|ene su zna~ajne razlike izme|u srednjih vrednosti za sva
prou~avana svojstva. Visoka varijabilnost je utvr|ena za broj klasova po m2, ne{to
ni`a za masu i broj zrna po klasu i visinu stabla, dok je najmanja varijabilnost
utvr|ena za du`inu klasa. Najve}a korelaciona zavisnost je utvr|ena izme|u visine
stabla i du`ine klasa i broja i mase zrna po kalsu.
VARIABILITY AND INTERDEPENDENCE OF YIELD COMPONENTS
IN MALTING BARLEY GROWN ON PSEUDOGLEY SOIL
The majority of our country is covered by semiarid regions which are, basically, less
favourable for malting barley production. In recent years, there has been a 30 percent
reduction in barley acreage which has not been followed by an increase in grain yield
per unit area, due to the fact that barley is also grown on low quality soils and in less
favourable semiarid regions. Four two-row winter barley genotypes, including Record, Jagodinac, Crystal and Premium, were evaluated in this study for variability of
yield components (spike number per m2, stem height, spike length, kernel number per
spike and kernel weight per spike). The study was conducted in 2008-2011 at the experimental field of the Secondary School of Agriculture in Kraljevo. The trial was set
up on acid pseudogley soil (pHH2O 4.5) in three replications. Significant differences
were determined between mean values of all traits analysed. Variability was high for
spike number per m2, somewhat lower for kernel weight per spike, kernel number per
spike and stem height, and lowest for spike length. The highest correlation was found
between stem height and spike length, as well as between kernel number per spike
and kernel weight per spike.
19
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KOMBINOVANI S1-TC-RRS UZ KORI[]ENJE
CMS I DIHAPLOIDA KOD KUKURUZA
Sofija Bo`inovi}, Jelena Van~etovi}, Dragana Ignjatovi}-Mici} i Nenad Deli}
Institut za kukuruz „Zemun Polje“, Beograd
Predstavljamo kombinovani S1-HS-RRS metod zasnovan na inbred testerima
(S1-TC-RRS), kao dugoro~ni oplemenjiva~ki pro gram, sa ciljem da pove}a
frekvenciju po`eljnih gena i odr`i geneti~ku varijabilnost dve geneti~ki opozitne
populacije. Ovaj metod u isto vreme popravlja dva razli~ita geneti~ka izvora, gde se
S1 familije, dobijene samooplodnjom fenotipski odabranih biljaka iz obe populacije,
ukr{taju sa opozitnim inbred testerom, radi selekcije na specifi~ne kombinacione
sposobnosti, per se, nakon ~ega se kroz test oglede vr{i selekcija S1 familija, tako|e,
per se. Izvestan procenat izabranih S1 familija se koristi za slede}i S1-TC-RRS
selekcioni ciklus. Metodom maj~inskih haploida iz odabranih S1 linija svakog
S1-TC-RRS ciklusa za kratko vreme se mogu dobiti 100% homozigotne inbred linije,
{to je od velike va`nosti za smanjenje tro{kova i pove}anje efikasnosti u semenskoj
proizvodnji, kao i za{titi autorskih prava. Ove elitne linije se mogu prevesti na cms
verziju (o~inski haploidi), {to je, tako|e, bitno za semensku proizvodnju.
COMBINED S1-TC-RRS WITH THE UTILISATION
OF CMS AND DIHAPLOIDS IN MAIZE
Herein, we present the com bined S1-HS-RRS method based on in bred test ers
(S1-TC-RRS) as a long-term maize breeding program, with the aim to increase the
frequency of favorable alleles and maintain genetic variability of two genetically opposite populations. This method improves two different genetic sources simultaneously, where S1 families, developed by selfing phenotypically selected plants from
both breeding populations, are crossed with opposite inbred testers for specific combining ability selection, per se, followed by test trials for selection of S1 families per se,
also. A certain percentage of the selected S1 families is used for the next TC-RRS selection cycle. With the method of maternal haploids from the selected S1 lines of each
cycle of S1-TC-RRS elite 100% homozygous inbred lines can be produced in a short
time, which is of great importance for expenses decrease and efficiency increase in
seed production, as well as, variety protection rights. This elite lines than can be converted to cms versions (paternal haploids), which is, also, important for their seed
production.
20
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ GENOTIPA I KALCIFIKACIJE NA
KONCENTRACIJU KADMIJUMA U KUKURUZU
Vlado Kova~evi}1, Domagoj [imi}2, Imre Kadar3,
Desimir Kne`evi}4 i Zdenko Lon~ari}1
3Research
1Poljoprivredni fakultet, Osijek, Hrvatska
2Poljoprivredni institut, Osijek, Hrvatska
Institute for Soil Science and Agricultural Chemistry, Budapest, Hungary
4Poljoprivredni fakultet, Le{ak
Kontaminacija kadmijumom (Cd) je ozbiljan ekolo{ki problem koji ograni~ava biljnu
proizvodnju i ima {tetan utjecaj na ljudsko zdravlje kroz hranidbeni lanac. Cilj rada je
analizirati utjecaj kalcizacije i hibrida na koncentraciju Cd u listu i zrnu kukuruza.
Rastu}e koli~ine (0,5 i 20 t ha-1) kre~a (73% CaO + 2-3% MgO + 21% vode) je
dodano u prolje}e 2006. Svaka parcela kalcizacije (veli~ina 414 m2) je podijeljena u
~etiri parcelice da bi se dobilo 4 ponavljanja. [est hibrida (H1 = Os298P, H2 =
Tvrtko303, H3 = Os499, H4 = Os444, H5 = Os596 and H6 = Os552) je posijano
po~etkom maja (osnovna parcela 24 m2). Listovi ispod klipa uzeti su u svilanju, a zrno
u zriobi. Razlike u koncentracijama Cd u suvoj materiji lista izme|u hibrida bile su u
rasponu od 0.040 to 0.160 mg Cd kg-1. H1 i H2 su se izdvajali od ostalih 4 hibrida
ve}im koncentracijama Cd (prosjeci 0.141, odnosno i 0.043 mg Cd kg-1). Me|utim,
koncentracije Cd u zrnu bile su znatno (ispod ranga detekcije: <0.02 mg Cd kg-1) i bez
opasnosti od kontaminacije kadmijem. Uslijed kalcizacije je koncentracija Cd u listu
2007. bila za 40% ni`a (0.097, odnosno 0.057 mg Cd kg-1).
Klju~ne re~i: hibridi kukuruza, kadmij, list, zrno, kalcizacija
GENOTYPE AND LIMING EFFECTS ON
CADMIUM CONCENTRATION IN MAIZE
Cadmium (Cd) contamination is a serious environmental problem that limits crop
production and has detrimental effects on human health through the food chain. Aim
of this study was testing liming and hybrid effects on Cd concentrations in leaf and
grain of maize. Increasing rates (0, 5 and 20 t ha-1) of lime (73% CaO + 2-3% MgO +
21% water) was applied in spring 2006. Each plot (size 414 m2) was divided in four
sub-plots for re ceiv ing four rep li cates. Six maize hy brids (H1 = Os298P, H2 =
Tvrtko303, H3 = Os499, H4 = Os444, H5 = Os596 and H6 = Os552) was sown at
beggining of May (basic plot 24 m2) of 2006 and 2007. The ear-leaves were collected
at silking and grain at maturity. Differences of leaf-Cd (on dry matter basis) was
found among the hybrids in range from 0.040 to 0.160 mg Cd kg-1. The H1 and H2
separated from remaining four hybrids by the higher leaf-Cd (means 0.141 and 0.043
mg Cd kg-1, respectively). However, grain-Cd was considerably lower (<0.02 mg Cd
kg-1) and out of serious food contamination effects. As affected by liming leaf-Cd in
2007 was for 40% lower (0.097 and 0.057 mg Cd kg-1, for the control and mean of two
liming treatments, respectively).
Key words: maize hybrids, cadmium, leaf, grain, liming
21
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA ANALIZA POPULACIJA KUKURUZA
NA TOLERANTNOST PREMA CRVENILU
Goran Bekavac, Bo`ana Purar, \or|e Jockovi},
Milisav Stojakovi}, Mile Ivanovi}, Bojan Mitrovi} i Ivica \alovi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Pojava crvenila kukuruza (CR) je u poslednjih desetak godina zna~ajno uticala na
proizvodnju kukuruza u Banatu, R. Srbija. Crvenilo se javljalo u intervalu od 10-90%
prouzrokuju}i sni`enje prinosa i zabrinutost proizvo|a~a. Kako ne postoje
komercijalni hibridi tolerantni prema CR, intenzivna istra`ivanja se vr{e u cilju
identifikacije odre|enog nivoa tolerantnosti u oplemenjiva~kim materijalima.
Tolerantnost prema CR je ocenjivana u tri geneti~ka pula (Lokalni, BSSS i LSC)
tokom 2009 i 2010. godine. Ogledi su postavljani na dve lokacije u epicentru pojave
CR. Svaki pul reprezentovale su po dve populacije u okviru kojih je testirano po 40 S2
familija. Osnovnu parcelicu sa~injavalo je 100 biljaka posejanih u dva reda na
kona~an sklop od 64000 bilj/ha. Analizirani su osetljivost familija prema crvenilu (%
biljaka sa tipi~nim CR simptomima na biljci i klipu), prinos zrna i sadr`aj vlage u zrnu.
Signifikantne razlike za osetljivost prema CR ustanovljene su izme|u geneti~kih
pulova. Najvi{a tolerantnost ustanovljena je u Lokalnom, zatim u LSC i na kraju
BSSS materijalu. Ovakvi rezultati bi mogli uticati na promenu oplemenjiva~kih
koncepta, posebno prema odre|enim tipovima materijala.
Klju~ne re~i: crvenilo, kukuruz, oplemenjivanje
GENETIC ANALYSIS OF CORN POPULATION
FOR TOLERANCE TO REDDENING
Several outbreaks of corn reddening (CR) in the last decade significantly affected
corn production in Banat region, R. Serbia. CR occurrence ranged from 10 to 90%
causing sizable yield loss and concern among farmers. Since there are no CR tolerant
commercial hybrids, intensive activities are ongoing to identify a certain level of tolerance within breeding materials. Three gene pools (Local, BSSS and LSC) were assessed for tolerance to CR during 2009 and 2010. Evaluation trials were set up at two
locations at the epicenter of corn reddening. Each pool was represented by two populations and 40 S2 families within each population were tested. The basic two-row plot
consisted of 100 plants was planted to a final density of 64,000 plants/ha. Data were
collected to CR susceptibility (% of plants with typical CR symptoms on plant and
the ear), grain yield, and grain moisture. Significant differences for CR susceptibility
were observed between tested pools. The highest tolerance to CR showed Local, followed by LSC and BSSS material. These findings could influence breeder’s preferences to some types of materials.
Key words: reddening, corn, breeding
22
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ZP HIBRIDI KAO SIROVINA ZA BIOETANOL
Milica Radosavljevi}1, Valentina Seme~enko1, Milan Stevanovi}1, Milomir Filipovi}1,
Zoran ^amd`ija1, Sne`ana Mladenovi} Drini}1 i Bojan Stipe{evi}2
1Institut za kukuruz „Zemun Polje“, Beograd
2Poljoprivredni fakultet Osijek, Osijek, Hrvatska
Cilj rada je ispitivanje mogu}nost kori{tenja pet ZP hibrida kukuruza kao sirovine za
proizvodnju etanola Najni`i sadr`aj skroba je imao hibrid ZP341 (69%) a najve}i
hibrid ZP704wx, 74.13%. Prinos skroba se kretao od 61,99 (ZP704wx) do 65,15
(ZP434), dok je iskoristljivost skroba bila u opsegu od 83,62 to 93,14%. Najve}i prinos
etanola, izra`en u % u odnosu na teoretski prinos, imao je hibrid ZP 434 (90,2%), a
najni`i hibrid ZP 544 (69,32%). Sli~an prinos etanola (pribli`no 79%) imaju hibridi
ZP 704wx i ZP 341, dok se sadr`aj skroba razlikovao kod ova dva hibrida. Prinos i
obnovljivost skroba su va`nija svojstva za proizvodnju etanola od sadr`aja skroba.
Hibrid ZP 434 je veoma pogodan za proizvodnju bietanola i skroba, po{to ima najve}i
prinos etanola kao i max prinos i obnovljivost skroba.
ZP HYBRIDS AS RAW MATERIAL FOR ETHANOL
The aim of this paper is study of the utility of five ZP maize hybrids as raw material
for ethanol production. The lowest strach content has hybrid ZP341 (69%) and the
higest hybrid ZP704wx, 74.13%. Starch yield varied from 61,99 (ZP704wx) to 65,15
(ZP434), while starch recovery varied from 83,62 to 93,14%. The higest ethanol yield,
expressed in % of the theoretical yield, after 34 hours of fermetation was obtained
with ZP 434, 90,2% and the lowest one with ZP544. Hybrids ZP 704 and ZP 341 have
similar ethanol yield, about 79%. These two hybrids significantly differ in starch content. The starch yield and starch recovery are more important traits for ethanol production than starch content. The hybrid ZP 434 is extremely suitable for the production of bioethanol and starch, as it had the highest ethanol yield, as well as, the maximum yield and starch recovery.
23
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ SORTE I GODINE NA KOMPONENTE
KVALITETA OZIME P[ENICE
Veselinka Ze~evi}1, Jelena Bo{kovi}1, Desimir Kne`evi}2 i Danica Mi}anovi}3
1Fakultet za biofarming,
2Poljoprivredni
Ba~ka Topola, Beograd
fakultet, Le{ak
3Privredna komora Srbije, Beograd
U radu je ispitivan tehnolo{ki kvalitet devet sorti ozime p{enice (Ana Morava,
Aleksandra, Toplica, KG-56S, Kruna, Planeta, Takov~anka, Vizija i Studenica).
Istra`ivanja su obavljena u trogodi{njem periodu (2006-2008) na oglednom polju
Centra za strna `ita u Kragujevcu. Analizirane su osobine kvaliteta zrna p{enice:
sedimentaciona vrednost, sadr`aj glutena i reolo{ke osobine bra{na i testa (apsorpcija
vode, razvoj testa, stabilnost testa, stepen omek{anja i indeks tolerancije na me{enje).
Ustanovljen je zna~ajan uticaj godine na vrednosti ispitivanih komponenti kvaliteta
zrna i bra{na. Ispitivane sorte p{enice su imale kvalitet na nivou prve i druge
kvalitetne klase. Analizom farinograma ustanovljeno je da bra{no ispitivanih sorti
pripada A2 i B1 kvalitetnim grupama. Rezultati su pokazali da sorte Ana Morava,
Aleksandra, Toplica i KG-56S imaju odli~an kvalitet zrna i bra{na. Analizom
varijanse su utvr|ene visoko zna~ajne razlike izme|u sorti i godina za ispitivane
komponente kvaliteta ozime p{enice.
Klju~ne re~i: p{enica, sorta, kvalitet, interakcija sorta-godina
THE INFLUENCE OF CULTIVAR AND YEAR ON
QUALITY PROPERTIES OF WINTER WHEAT
Quality of nine winter wheat cultivars (Ana Morava, Aleksandra, Toplica, KG-56S,
Kruna, Planeta, Takov~anka, Vizija and Studenica) was studied in this paper. Field
experiment was conducted in three growing seasons (2006-2008) on the experimental
field of Small Grains Research Centre of Kragujevac. Zeleny sedimentation value,
wet gluten content and rheological properties of flour and dough (water absorption,
dough development time, dough stability time, dough weakness, and mixing tolerance
index) were analyzed.
There was a significant effect of year on the value of studied quality components of
grain and flour. The investigated wheat cultivars had quality at first and second quality class. Farinograph properties have shown that flour of analyzed cultivars belonged
to A2 and B1 qual ity groups. The results showed that the va riety Ana Morava,
Aleksandra, Toplica and KG-56S have excellent grain and flour quality. Analysis of
variance showed highly significant differences among cultivars and years for investigated quality properties.
Key words: wheat, cultivar, quality, cultivar-year interaction
24
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENOTIPSKA SPECIFI^NOST NEKIH SVOJSTAVA
OZIME P[ENICE I NJIHOV UTICAJ NA PRINOS
Neboj{a Deleti}, Slavi{a Stojkovi}, Slavi{a Gud`i}, Vladan \uri} i Miroljub Aksi}
Poljoprivredni fakultet, Le{ak
U radu su prikazani rezultati dvogodi{njih ispitivanja genotipske specifi~nosti nekih
parametara akumulacije azota i komponenti prinosa, kao i njihov pojedina~ni i
zdru`eni uticaj na prinos po biljci, kod dvadeset doma}ih sorata ozime p{enice.
Uo~ene su zna~ajne razlike izme|u ispitivanih genotipova u svim prou~avanim
svojstvima. Koeficijent varijacije se kretao od 6,81% za masu 1000 zrna do 12,91% za
ukupnu akumulaciju azota. Klaster analiza je pokazala podelu ispitivanih genotipova
u dve grupe, od kojih se ve}a grupa dalje deli na vi{e podgrupa. Dobra definisanost
grupa zna~i da obrazac variranja ovih svojstava predstavlja karakteristi~no svojstvo
genotipa. Vi{estruka regresiona analiza je pokazala da sva ~etiri ispitivana svojstva
pokazuju zna~ajan efekat na prinos zrna. Najja~i efekat na prinos zrna je imao broj
zrna u klasu, sa standardizovanim regresionim koeficijentom (â) od 0,535.
Korigovana vrednost R2 (0,984) pokazuje da je 98,4% od ukupnih varijacija u prinosu
zrna obja{njeno dejstvom ~etiri ispitivana svojstva. Kada se posmatra biolo{ki prinos,
samo ukupna akumulacija azota je bila zna~ajna. â vrednost za ukupnu AN je bila
0,713, a korigovana R2 vrednost 0,787.
Klju~ne re~i: p{enica, azot, prinos, regresija, klaster analiza
GENOTYPIC SPECIFICITY OF SOME WINTER WHEAT
TRAITS AND THEIR EFFECT ON GRAIN YIELD
This paper presents the two year results of a study dealing with genotypic specificity
of some nitrogen accumulation parameters and yield components, as well as their individual and joint influence on grain yield per plant, in twenty Serbian winter wheat
cultivars. There were significant differences among the investigated cultivars regarding the all studied traits. Coefficient of variation ranged from 6.81% for 1000 grain
mass to 12.91% for total nitrogen accumulation. Cluster analysis showed the studied
genotypes divided into two clusters, where larger one was further divided into several
smaller clusters. Good definition of clusters is a sign that these traits’ pattern is a distinctive property of a genotype. Multiple regression analysis showed that the all four
studied traits had significant effect on grain yield. The greatest effect was expressed
by number of grain per spike, where standardized regression coefficient (â) was 0.535.
Adjusted R2 value (0.984) showed that 98.4% of the observed variation in grain yield
was explained by the studied four traits. When biological yield is regarded, only total
NA was significant. â value for total NA was 0.713, and adjusted R2 was 0.787.
25
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
INTENZITET I [email protected] NALIVANJA ZRNA
SORTI P[ENICE RAZLI^ITOG POREKLA
Bojan Jockovi}, Novica Mladenov, Nikola Hristov i Vladimir A}in
Institut za ratarstvo i povrtarstvo, Novi Sad
Intenzitet i du`ina nalivanja zrna su va`ni parametri u razvoju biljke, jer oni
generalno odre|uju adaptabilnost genotipa u uslovima uspevanja. Intenzitet
nalivanja zrna predstavlja brzinu nakupljanja suve materije u zrnu tokom perioda
nalivanja zrna. Du`ina nalivanja zrna je period od cvetanja do `etvene zrelosti (vlaga
u zrnu 13%). U cilju ispitivanja intenziteta i du`ine nalivanja zrna p{enice, odabrano
je 8 genotipova (Sloga, Jugoslavija, Pesma, Prima, Nevesinjka, Balaton, Gk-Zugoly,
Bankut 1205) razli~itog porekla. Ogled je izveden na Rimskim [an~evima u
vegetacionoj sezoni 2009/2010. Dobijeni rezultati su pokazali da se sorte razlikuju u
ovim osobinama, ali i da se mogu svrstati u dve grupe. Prva grupa su sorte koje su
imale jednaku du`inu a razli~it intenzitet nalivanja zrna, a druga grupa su sorte sa
razli~itom du`inom i skoro identi~nim intenzitetom nalivanja zrna. Jednaku du`inu a
razli~it intenzitet nalivanja zrna imale su sorte Sloga i Jugoslavija, Balaton i
Gk-Zugoly, a razli~itu du`inu i skoro identi~an intenzitet nalivanja zrna imale su sorte
Pesma i Bankut 1205, Prima i Nevesinjka.
Klju~ne re~i: p{enica, intenzitet nalivanja zrna, du`ina nalivaja zrna
RATE AND DURATION OF GRAIN FILLING OF
DIFFERENT ORIGIN WHEAT CULTIVARS
Rate and duration of grain filling are important parameters in the development of
plants, because they generally determine the adaptability of the genotype in growing
conditions. Grain filling rate is expressed as a speed of dry matter accumulation during grain filling period. Grain filling duration is the time between anthesis and harvest
maturity (13% moisture in grains). In order to examine the rate and duration of grain
filling of wheat, eight genotypes were selected (Sloga, Jugoslavija, Pesma, Prima,
Nevesinjka, Balaton, Gk-Zugoly, Bankut 1205) with different origin. Field trial was
set up at Rimski Sancevi in 2009/2010 season. The results showed that the varieties
differ in these traits, but that they can be classified into two groups. The first group of
varieties that have had the same duration and different rate of grain filling, and another group of varieties with different duration and almost identical rate of grain filling. Same du ra tion and dif fer ent rate of grain fill ing had a va ri ety Sloga and
Yugoslavija, Balaton and Gk-Zugoly, and different duration and almost identical rate
of grain filling had a variety Pesma and Bankut 1205, Prima and Nevesinjka.
Key words: wheat, grain filling rate, grain filling duration
26
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NASLE\IVANJE [email protected] KLASA KOD HIBRIDA
P[ENICE F3, F4 I F5 GENERACIJE POTOMSTVA PRIMENOM
RAZLI^ITIH METODA SELEKCIJE
Sne`ana Jankovi}1, Slaven Prodanovi}2 i Violeta Mandi}3
1Institut
za primenu nauke u poljoprivredi, Beograd
2Poljoprivredni fakultet, Zemun
3Institut za sto~arstvo, Zemun
U radu je analiziran na~in nasle|ivanja du`ine klasa kod hibrida p{enice F3, F4 i F5
generacija dobijenih primenom pedigre, bulk i pedigre iz bulka metodom selekcije.
Hibridi p{enice dobijeni su ukr{tanjem pet sorti, po M x N metodu. Sorte Briscard,
Carifen 12 i Rescler kori{}ene su kao majke, a sorte Francuska i PKB-Prelivka kao
o~evi. Od {est F1 hibrida (3x2) proizvedena su potomstva F2 generacije. Selekcija iz F2
generacije trajala je od 1996 do 1999, a u 2000. godini postavljeni su poljski ogledi sa
celokupnim materijalom u Institutu „PKB INI Agroekonomik“ u Padinskoj Skeli.
Du`ina klasa se pove}ava u odnosu na roditelja sa du`im klasom kod nekih od
generacija potomstva kod pet kombinacija: Briscard x PKB-Prelivka, Carifen 12 x
Francuska, Carifen 12 x PKB-Prelivka, Rescler x Francuska, Rescler x PKB-Prelivka,
osim kod potomstva Briscard x Francuska. Pri nasle|ivanju du`ine klasa jedino je
hibridna kombinacija Rescler x Francuska u F5 generaciji nasledila du`inu klasa od
slabijeg roditelja pri pedigre i bulk metodu selekcije, dok su ostali hibridi nasledili
du`inu klasa od boljeg roditelja pri svim metodama selekcije.
Klju~ne re~i: du`ina klasa, metodi selekcije, nasle|ivanje, p{enica
INHERITANCE OF SPIKE LENGTH IN F3, F4 AND F5 WHEAT
HYBRIDS OBTAINED BY DIFFERENT SELECTION METHODS
In this study, the mode of inheritance of spike length of F3, F4 and F5 wheat hybrids
obtained by pedigree, bulk and pedigree after bulk selection was analyzed.
Wheat hybrids were produced after crossing five varieties by M x N method. Three
varieties were used as a female parent (Briscard, Carifen 12 and Rescler) and two as a
male component (Francuska and PKB-Prelivka). Descendents in F2 generation were
produced from 6 F1 hybrids (3 x 2). Selection after F2 generation were undertaken
from 1996 to 1999, while in 2000 the field experiments with all material were set up at
the Institute „PKB INI Agroekonomik“, in Padinska Skela.
Spike length is increased as compared to parents with longer spike in some generations of offspring combination: Briscard x PKB-Prelivka, Carifen 12 x Francuska,
Carifen 12 x PKB-Prelivka, Rescler x Francuska, Rescler x PKB-Prelivka, except in
the offspring Briscard x Francuska. In the inheritance of spike length by pedigree and
bulk selection, only hybrid combination Rescler x Francuska in F5 generation inherited the length of the parent with lower value, while other hybrids inherited the spike
length of the better parent by all selection methods.
27
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA DIVERGENTNOST SORTI JE^MA U SRBIJI
Vesna Kandi}1, Dejan Dodig1, Gordana [urlan Momirovi}2,
Miroslav Zori}3, Kamenko Bratkovi}4 i Milan Stevanovi}1
1Institut za kukuruz “Zemun Polje”, Beograd
2Poljoprivredni fakultet, Zemun
3Tehnolo{ki fakultet, Novi Sad
4Centar za strna `ita Kragujevac, Kragujevac
U radu je kori{}eno 15 SSR markera, mikrosatelita, kako bi se analizirala geneti~ka
divergentnost 25 sorti je~ma, stvorenih u pet Instituta u Srbiji, u periodu od 1977 do
2009. godine. Broj alela po lokusu kretao se od 1 do 5, u proseku 2.4. Indeks polimorfizma (PIC) iznosio je od 0.141 do 0.549, dok je frekvencija alela bila od 0.435 do
1.000, u proseku 0.791. Divergentnost gena je bila niska 0.286. Analiza molekularne
varijanse (AMOVA) za tip klasa, oplemenjiva~ku instituciju i godinu priznavanja
pokazala je najve}u geneti~ku varijabilnost unutar oplemenjiva~kih institucija 94.3%,
a najmanju izme|u institucija 5.7%. Na dvodimenzionalnom PcoA plotu dobro su se
razdavojile dvorede i {estorede sorte. Tako|e, klaster analizom je 25 ispitivanih sorti
na osnovu tipa klasa podeljeno u dve grupe, osim u slu~aju sorti PKB Pivan I Kristal
koje su se iako dvorede na{le u grupi sa {estoredim sortama. Kako bi se izbegli
potencijalni rizici od prevelikog su`avanja geneti~ke divergentnosti, moglo bi se
preporu~iti uvo|enje roditeljskih linija razli~itog porekla u programe oplemenjivanja
je~ma u Srbiji.
Klju~ne re~i: je~am, SSR markeri, geneti~ka divergentnost
GENETIC DIVERSITY OF SERBIAN BARLEY VARIETIES
In this study 15 simple sequence repeats, SSRs markers were used to analyze genetic
diversity among 25 barley varieties, released from 1977 until 2009, in a five Serbian
Breeding Institutes. The number of alleles per locus ranged from 1 to 5 with an average of 2.4. The polymorphism information content (PIC) ranged from 0.141 to 0.549,
while frequencies of alleles ranged from 0.435 to 1.000 with an average of 0.791. Gene
diversity was low 0.286. The results of AMOVA for row type, breeding institution and
year of release group effect, showed that the highest genetic variation was within
breeding institutions 94.3%, and the smallest among institution 5.7%. Two dimensional PcoA plot showed a good separation between two and six-rowed varieties.
Cluster analysis of the similarity data grouped the 25 studied varieties into two groups
according to their spike type except for PKB Pivan and Kristal, which are two rowed
types but clustered among six rowed group. To avoid the potential risks of too narrow
genetic diversity, introduction of parental lines from different origins could be recommended for barley breeding programs in Serbia.
Key words: barley, SSR markers, genetic diversity
28
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA ANALIZA OSOBINA KLASA U
UKR[TANJU DVOREDOG I VI[EREDOG JE^MA
Milomirka Madi}1, Desimir Kne`evi}2, Aleksandar Paunovi}1 i Dragan \urovi}1
1Agronomski fakultet, ^a~ak
2Poljoprivredni fakultet, Le{ak
Za ispitivanje, na~ina nasle|ivanja i komponenti geneti~ke varijanse du`ine klasa,
broja i mase zrna po klasu analiziran je materijal ~etiri roditeljska genotipa i njihovi
hibridi F1 i F2 generacije dobijeni nepotpunim dialelnim ukr{tanjem. Za ukr{tanja
odabrani su genotipovi vi{eredog je~ma HVW-247, Partizan i dvoredog KG-15 i
NS-293 na bazi koncepta svojstva pri ~emu su roditelji bili divergentni za du`inu
klasa, broj i masu zrna po klasu.
Srednje vrednosti du`ine klasa u F1 i F2 generaciji su intermedijarne ili se pribli`avaju
roditelju sa ve}om du`inom klasa, a na~in nasle|ivanja je parcijalna dominacija,
dominacija ili superdominacija, zavisno od kombinacije ukr{tanja.
U ukr{tanju {estoredi x {estoredi i dvoredi x dvoredi u nasle|ivanju broja i mase zrna
po klasu preovladava dominacija ve}eg broja zrna po klasu, odnosno ve}e mase zrna
po klasu, a u ukr{tanju {estoredi x dvoredi parcijalna dominacija.
GENETIC ANALYSIS OF SPIKE TRAITS IN
TWO-AND MULTIPLE-ROWED BARLEY CROSSES
The mode of inheritance and genetic variance components for spike length, grain
number per spike and grain weight per spike were analysed in four parental genotypes and their hybrids of F1 and F2 generations obtained by incomplete diallel crossing. Genotypes of HVW-247 and Partisan multiple-rowed barley and KG-15 and
NS-293 two-rowed barley were selected for the crossing based on the trait concept
with the parents being divergent in terms of spike length, grain number per spike and
grain weight per spike.
The mean values of spike length in F1 and F2 generations were intermediate or close
to those of the parent having longer spikes, whereas the mode of inheritance was partial dominance, dominance or overdominance, depending on the cross combination.
The six-rowed x six-rowed and two-rowed x two-rowed cross ing com bi na tions
showed dominance of a higher grain number per spike and higher grain weight per
spike in the inheritance of grain number and grain weight per spike, whereas the
six-rowed x two-rowed crossing was predominated by partial dominance.
29
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POVEZANOST KOMPONENTI PRINOSA JE^MA GAJENOG
U USLOVIMA RAZLI^ITIH DOZA ISHRANE AZOTOM
Desimir Kne`evi}1, Dejan Dodig2, Vesna Kandi}2, Gordana Brankovi}3,
Gordana [urlan-Momirovi}3, Jelica @ivi}4 i Ivica Stan~i}4
1Poljoprivredni fakultet, Le{ak
2Institut za kukuruz „Zemun Polje“, Beograd
3Poljoprivredni fakultet, Zemun
4Visoka
poljoprivredno-prehrambena {kola, Prokuplje
U radu su izu~avane komponente prinosa (visina biljke, broj klasova/m2, du`ina
klasa, broj klasaka po klasu, broj zrna u klasu, masa zrna u klasu, prinos zrna) i
njihova me|uzavisnost kod ~etiri genotipa ozimog je~ma (G-3003, G-3020,
G-3007-1/02 i G-3019) gajenih u uslovima ishrane primenom razli~itih doza azota
(kontrola=0, N1=20, N2=40 and N3=60 kg ha-1). Za ocenu me|uzavisnosti je vr{eno
izra~unavanje koeficijenta korelacije izme|u ispitivani osobina. Ustanovljena je
visoka me|uzavisnost izme|u ispitivanih osobina. Sve ispitivane osobine su imale
najve}i koeficijent korelacije sa prinosom zrna (visina biljke-0,97; broj
klasova/m2-0,95; broj klasaka po klasu-0,95; broj zrna u klasu-0,95; masa zrna u
klasu-0,82) dok je du`ina klasa imala najve}u korelaciju sa masom zrna po klasu
(0,82). Dobijene vrednosti ukazuju visoko zna~ajnu me|usobnu uslovljenost.
ispitivanih osobina sa ukupnim prinosom zrna. Najmanja korelacija je ustanovljena
izme|u visine biljke i du`ine klasa ~iji je koeficijent korelacije iznosio 0,62.
Klju~ne re~i: je~am, prinos, korelacija, stablo, osobine klasa, genotipovi
RELATIONSHIP AMONG YIELD COMPONENTS IN BARLEY CULTIVATED
IN CONDITIONS OF DIFFERENT NITROGEN DOSES NUTRITION
The paper studied the yield components (plant height, number klasova/m2, spike
length, number of spikelets per spike, grain number per spike, grain weight in the ear,
grain yield) and their interdependence in the four winter barley genotypes (G-3003,
G -3020, G-3007-1/02 and G-3019) grown in terms of nutrition using different doses of
nitrogen (control=0, N1=20, N2=40 and N3=60 kg ha-1). For estimation of interdependence among anlyzed traits, the correlation coefficient was computed. The strong interdependence between the traits was established. All tested traits had the highest
cor re la tion co ef fi cient with the grain yield (plant height-0. 97, num ber of
spike/m 2 -0.95; num ber of spikelets per spike-0. 95, the num ber of kernels per
spike-0.95; mass of grain per spike- 0.82) while the length of spike, had the highest
correlation with the mass of grains per spike (0.82). The obtained values indicate a
highly significant mutual conditionality of the traits with total grain yield. The smallest correlation was established between plant height and spike length of which is the
correlation coefficient was 0.62.
Key words: barley, yield, correlation, stem, spike traits, genotypes
30
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PROCENA VREDNOSTI NOVIH NS HIBRIDA
KUKURUZA PRIMENOM GGE BIPLOT ANALIZE
Milisav Stojakovi}, Bojan Mitrovi}, Du{an Stanisavljevi},
Mile Ivanovi}, Aleksandra Nastasi} i \or|e Jockovi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Analizirani su dvogodi{nji rezultati ispitivanja va`nijih osobina 20 novih hibrida
kukuruza FAO 600 grupe zrenja u pore|enju sa standardom. Detaljnije su
komentarisani podaci koji se odnose na hibride NS 6683, NS 6686, NS 281633 i NS
396432. Za ispitivanje prinosa zrna, sadr`aja vlage u zrnu u berbi, ~vrsto}e stabla i
otpornosti na bolesti i {teto~ine postavljen je ogled u polju po RCB dizajnu u 4
ponavljanja na 6 lokaliteta u 2009. i na 5 lokaliteta u 2010. godini. Rezultati su
predstavljeni preko GGE biplotova u cilju rangiranja hibrida u odnosu na standard
uzimaju}i u obzir prisustvo interakcije genotip-spoljna sredina i identifikacije
najprinosnijih genotipova u razli~itim sredinama. Kod novih NS hibrida kukuruza je
ustanovljen vi{i prinos zrna od standarda za 0,883 do 1,720 tha-1, ni`i sadr`aj vlage u
zrnu za 0,85 do 2,54%, ve}a ~vrsto}a stabla i otpornost prema bolestima i insektima u
odnosu na standard. Identifikovani su i takozvani idealni lokaliteti za pojedine
hibride {to mo`e biti od koristi prilikom njihove rejonizacije.
Klju~ne re~i: hibridi kukuruza, prinos zrna, interakcija genotip-spoljna sredina, GGE
biplot analiza
ESTIMATION OF NEW NS MAIZE HYBRIDS
USING GGE BIPLOT ANALISYS
Testing results of twenty new maize hybrids FAO 600 maturity group were analysed
and compared with standard. Testing results of four new maize hybrids, NS 6683, NS
6686, NS 281633 and NS 396432 were analysed with greater attention. For testing of
grain yield, grain moisture content, stalk strength and resistance to pests and diseases,
RCBD field trials were conducted in two years with four replications across six locations in 2009. and five locations in 2010. The results are represented by GGE biplots
with the purpose of ranking hybrids compared to the standard and identification of
the highest yielding genotypes in different environments taking into account the presence of genotype by environment interaction. New NS maize hybrids achieved a
higher yield than the stan dard for 0,883-1,720 tha-1 , lower moisture content for
0,85-2,54%, higher stalk strength and higher resistance to diseases and pests than the
standard. In addition, so called ideal environments were identified for specific NS hybrids which can be useful in their regional distribution.
Key words: maize hybrids, grain yield, genotype by environment interaction, GGE
biplot analisys
31
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KOMBINACIONE SPOSOBNOSTI INBRED LINIJA KUKURUZA
RAZLI^ITIH CIKLUSA REKURENTNE SELEKCIJE
Mile Se~anski1, Tomislav @ivanovi}2, Gordana [urlan-Momirovi}2,
Goran Todorovi}3, Milosav Babi}1 i Jelena Srdi}1
3Institut
1Institut za kukuruz “Zemun Polje”, Beograd
2Poljoprivredni fakultet, Zemun
za prou~avanje lekovitog bilja, „Dr Josif Pan~i}“, Beograd
Za prou~avanje u ovom radu odabrane su inbred linije kukuruza dobijene iz razli~itih
ciklusa rekurentne selekcije dve sinteti~ke populacije: BSSS i BSCB1. Ispitivana je
varijabilnost ovih linija i njihovih hibrida, heterozis i op{te i posebne kombinacione
sposobnosti. Hibridi su pokazali ve}e prose~ne vrednosti prinosa zrna u odnosu na
linije obzirom da pri inbridingu dolazi do depresije ovih osobina kod linija. Najve}u
vrednost heterozisa ispoljio je hibrid B14 x B37. Analizom varijanse za prinos
ustanovljeno je da postoje visoko zna~ajne pozitivne vrednosti OKS i PKS za ovu
osobinu. Po{to je OKS pokazatelj aditivne geneti~ke varijanse a PKS neaditivne
(dominacija i epistaza) utv|eno je da za visinu prinosa zrna glavni uticaj ima
neaditivno dejstvo gena. Linije ZPL1 i ZPL2 ispoljile su najbolju op{tu kombinacionu
sposobnost a hibridi ZPL2 x B73, ZPL1 x ZPL2, ZPL1 x B90 i ZPL1 x B91 su sa
zna~ajnim vrednostima PKS u obe godine ispitivanja. Ove hibridne kombinacije
uklju~uju oba roditelja sa dobrim OKS ili jednog roditelja sa dobrim OKS i drugog sa
lo{im OKS vrednostima.
Klju~ne re~i: heterozis, kukuruz, kombinaciona sposobnost, prinos
COMBINING ABILITIES OF MAIZE INBRED LINES OF
DIFFERENT CYCLES OF RECURRENT SELECTION
Maize inbred lines derived from different cycles of recurrent selection of two synthetic populations (BSSS and BSCB1) were selected for these studies. Variability of
these inbreds and their hybrids, heterosis, general and special combining abilities
were studied. Hybrids had higher values for grain yields than their inbreds, as a depression of these traits occurs in inbreds in the process of inbreeding. The highest
value of heterosis was recorded in the hybrid B14 x B37. Highly significant positive
values of GCA and SCA for yield were established by the analysis of variance for this
trait. As GCA is a parameter of additive genetic variance and SCA is a parameter of
non-additive genetic variance (dominance and epistasis), it was determined that the
level of grain yield was mainly affected by additive gene effects. Inbreds ZPL1 and
ZPL2 expressed the highest general combining ability, while hybrids ZPL2 x B73,
ZPL1 x ZPL2, ZPL1 x B90 and ZPL1 x B91 had significant values of SCA in both
years of investigation. These hybrid combinations include both parents with high
GCA or one parent with high GCA and other with low GCA values.
Key words: heterosis, maize, combining ability, yield
32
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KORELACIJA IZMEDJU HETEROZISA, KOMBINACIONE SPOOBNOSTI
I GENETI^KE DISTANCE INBRED LINIJA KUKURUZA
Milomir Filipovi}, Aleksandar Radoj~i}, Marija Kostadinovi},
Danijela Risti}, Dragan Kova~evi} i Sne`ana Mladenovi} Drini}
Institut za kukuruz Zemun Polje, Beograd
U radu je ispitivana korelacija izme|u heteroticnog efekta i kombinacionih
sposobnosti sa genetickom distancom izracunatom na osnovu polimorfizma SSR
markera. Sest roditeljskih linija i 30 F1 ukrstanja su analizirana u ogledu po RCB
dizajnu sa cetiri ponavljanja u dve gustine u toku tri godine. Dvadeset i devet SSR
markera su identifikovala 75 alela, prosecno 2.6 alela po lokusu. Geneticka distanca
izracunata po Jaccardu je bila u opsegu od 0.52 do 0.83 (ZPL142 x ZPL17/5). Linije
(ZPL142 x ZPL17/5). Klaster analizom linije su grupisane u dva klastera, shodno
poreklu. Najsrodnije u bile linije ZPL17/5 i ZPL 173/3. Ova kombinacija je imala
najnizi prinos zrna i SCA. Dobijena je pozitivna srednja, znacajna korelacija izme|u
specificnih kombinacionih sposobnosti i geneticke distance kao i izme|u heterozisa
za prinos zrna i geneticke distance.
THE CORRELATION BETWEEN HETEROSIS, COMBINING
ABILITY AND GENETIC DISTANCE OF MAIZE INBRED LINES
The correlations between grain yield heterosis, combinig ability and genetic distance
based on SSR markers were investigated. Six parent inbred lines and 30F1 crosses
were analysed by RCB design with four replication in two densities in three years.
Twenty nine SSR markers identified 75 alleles with an average 2,6 alleles per locus.
Genetic distance based on Jaccard coefficient ranged from 0,52 to 0,83 (ZPL142 x
ZPL17/5). The lowest genetic distance was established between ZPL17/5 and ZPL
173/3. This combination has the lowest grain yield and SCA value. A positive, midlle
and significant correlation between specific combining ability and genetic distance as
well as between grain yield heterosis and genetic distance was obtained.
33
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POREDJENJE GENETI^KIH DISTANCI (GD) LINIJA KUKURUZA
DOBIJENIH NA OSNOVU MORFOLO[KIH I MOLEKULARNIH MARKERA
Milosav Babi}1, Vojka Babi}1, Gordana [urlan-Momirovi}2,
Nenad Deli}1 i Milomir Filipovi}1
1Institut za kukuruz „Zemun Polje“, Beograd
2Poljoprivredni fakultet, Zemun
Zbog nepoznatog mehanizma geneti~ke kontrole i velikog uticaja spoljne sredine u
procesu ekspresije svojstava, o morfolo{kim markerima se ~esto govori kao o nepouzda nim pokazateljima geneti~kih odnosa. Morfolo{ka karakterizacija 19 linija
kukuruza je ura|ena po UPOV deskriptoru dok je molekularna karakterizacija
ura|ena RAPD markerima. Na osnovu ocena fenotipa po UPOV deskriptoru
izra~unati su kvadrati euklidskog rastojanja na osnovu kojih je formirana matrica
morfolo{kih sli~nosti. Na osnovu prisustva-odsustva traka na gelovima u RAPD
nanalizi izra~anati su koeficijenti geneti~kih sli~nosti po Jaccardu. Nakon
standardizacije podataka vr{ena su pore|enja morfolo{kih i geneti~kih sli~nosti
ispitivanih linija kukuruza. Korelacije su bile visoke i kretale su se od 0.47 za liniju L
217 do 0.76 za liniju L 86. Prose~na vrednost korelacija za sve ispitivane linije je
iznosila 0.64.
Tako|e su rezultati klaster analize kako za molekularne tako i za morfolo{ke
markere bili u visokoj saglasnosti sa pedgre podacima. Degradiranjem merne skale sa
skalnog na ordinarni nivo merenja, kod morfolo{kih markera (po UPOV
deskriptoru) je ubla`en efekat spoljne sredine i na taj na~in su se rezultati
morfolo{kih markera pribli`ili rezultatima molekularnih makera u proceni GD inbred linija kukuruza.
Klju~ne re~i: GD, molekularni i morfolo{ki markeri
COMPARISON OF GENETIC DISTANCES (GD) OF MAIZE INBREDS
DEVELOPED ON THE BASIS OF MORPHOLOGICAL AND MOLECULAR
MARKETS
Due to an unknown mechanism of genetic control and great environmental effects in
the process of trait expression, molecular markers are often considered unreliable indicators of genetic relationships. Morphological characterisation of 19 maize inbreds
was done according to the UPOV descriptor, while molecular characterisation was
performed with RAPD markers. Based on the estimation of phenotypes according to
the UPOV descriptor, the squared Euclidean distance was calculated and then on the
basis of this distance a morphological similarity matrix was formed. Jaccard similarity
coefficients were calculated on the basis of presence-absence of bands on gels in the
RAPD analysis. When data were standardised, the comparison between morphological and genetic similarity of observed maize inbreds was done. The correlations were
high and varied from 0.47 (inbred L 217) to 0.76 (inbred L 86). The average value of
correlations for all studied inbreds amounted to 0.64.
Furthermore, the results of the cluster analysis for both markers, molecular and morphological, had high concordance with pedigree data. Environmental effects were decreased in morphological markers (according to the UPOV descriptor) by degrading
a measurement scale from a scale to an ordinal level of measurement and in such a
way results of morphological markers approached results of molecular markers in the
estimation of GD of maize inbred lines.
34
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KI POTENCIJAL RODNOSTI KUKURUZA U SRBIJI
Jovan Pavlov, Nikola Gr~i}, Zoran ^amd`ija, Milan Stevanovi},
Milo{ Crevar i Sofija Bo`inovi}
Institut za kukuruz ”Zemun Polje”, Beograd
U Srbiji kukuruz zauzima najve}e povr{ine od svih useva. Istorijat gajenja kukuruza u
Srbiji se mo`e podeliti u vi{e faza, od prve introdukcije kukuruza, preko formiranja
prvih lokalnih populacija, dalje introdukcije inostranih populacija do po~etka gajenja
komercijalnih hibrida nastalih ukr{tanjem inbred linija. Me|utim kako je ostvaren
prinos kukuruza rezultat sadejtsva agroekolo{kih faktora, primenjenih agrotehni~kih
mera i geneti~kog potencijala rodnosti neophodno je pa`ljivo definisati doprinos
genetike. Iz toga razloga potrebno je uporedo pratiti dinamiku i promene u primeni
agrotehni~kih mera (|ubrenje, na~in setve, obrada zemlji{ta) i promene u genetici
gajenih hibrida kroz vremenski period. Tako|e treba prou~iti i promene svih osobina
hibrida od zna~aja za geneti~ki potencijal rodnosti i primenu agrotehni~kih mera. Od
1950 postoji trend jak pove}anja prinosa kukuruza uporedo sa razvojem hibridnog
sortimenta i inteziviranjem agrotehnike. Tokom 1990-ih primetan je pad u
ostvarenim prinosima {to je pre svega rezultat manjeg ulaganja u agrotehniku tako
da, do danas u Srbiji nisu sustignuti prinosi iz 1980-ih i pored gajenja u ve}oj meri
superiornijih hibrida kukuruza.
Klju~ne re~i: Kukuruz, geneti~ki potencijal rodnosti, agrotehnika
GENETIC YIELD POTENTIAL OF MAIZE IN SERBIA
In Serbia, maize occupies the largest area of all crops. The history of corn growing in
Serbia can be divided into several phases: the first introduction of maize, the formation of the first local population, further introduction of foreign population and the
cultivation of hybrids obtained by crossing inbred lines. However as yield is achieved
as a result of agroecological factors, applied agro-technical measures and genetic
yield potential, it is necessary to carefully define the contribution of genetics. For this
reason it is necessary to simultaneously monitor the dynamics and changes in the application of agro-technical measures (fertilization,, method of sowing, soil) and
changes in genetics of hybrids grown over time. Changes in other traits of importance
for the hybrid genetic yield potential and application of agro-technical measures
should also be examined. Since 1950, maize yields increased with the development of
hybrids and intesification of technology. However in 1990s there has been a decline in
obtained yields, due to lower investment in technology so that 1980s yields arent
reached today in Serbia, despite growing to a greater extent superior hybrids.
Key words: Maize, gentic zield potential, technology
35
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PARAMETRI VARIJABILNOSTI KOMPONENTI PRINOSA KOD
RAZLI^ITIH TIPOVA POTOMSTAVA JEDNE F3 POPULACIJE KUKURUZA
Slavi{a Stojkovi}, Neboj{a Deleti}, Miroljub Aksi}, Dragoljub Bekovi} i Vladan
\uri}
Poljoprivredni fakultet, Le{ak
U radu je ispitivana F3 populacija kukuruza 2002-30 dobijena u Zavodu za kukuruz
Instituta za ratarstvo i povrtarstvo u Novom Sadu. Najpre je oko 150 biljaka
navedene populacije samooplo|eno i istovremeno ukr{teno sa testerom 1491x1496,
da bi se dobila naspramna S1 i HS potomstva. Nakon berbe, metodom slu~ajnog
uzorka odabrano je po 41 S1 i HS potomstava za oglede koji su obavljeni u Aleksincu,
Leskovcu i Kru{evcu, {to ukupno ~ini tri okru`enja, po RCBD metodi.
Genetske varijanse su bile zna~ajno ve}e kod S1 potomstava za sva svojstva osim za
procenat poleglih i slomljenih biljaka i broj redova zrna. Fenotipske varijanse su
pratile isti trend. Dobijene vrednosti koeficijenta heritabilnosti u {irem smislu su bile
zna~ajno vi{e kod S1 u odnosu na HS potomstva. Niske vrednosti heritabilnosti
dobijene su samo za procenat poleglih i slomljenih biljaka. Klaster analiza nije
pokazala ve}e razlike izme|u S1 i HS potomstava, ni u distancama ni u definisanosti
grupa, iako su postojale zna~ajne razlike u genetskoj varijansi za ve}inu ispitivanih
svojstava. Ipak, primetne su ne{to manje distance izme|u HS potomstava.
Klju~ne re~i: kukuruz, varijabilnost, prinos, komponente prinosa
VARIABILITY PARAMETERS FOR YIELD COMPONENTS OF DIFFERENT
PROGENY TYPES IN AN F3 MAIZE POPULATION
This paper presents the investigation of F3 population 2002-30, created in the Department of Maize, Institute of Field and Vegetable Crops, Novi Sad. At first, about 150
plants of the studied population were selfed and simultaneously crossed with the
tester 1491x1496, in order to get complementary S1 and HS progenies. After the harvest, 41 progenies of each type were selected by the random sample method for the
trials carried out in Aleksinac, Leskovac, and Kru{evac, as three studied environments, in RCB design.
Genetic variances were higher in S1 progenies, with the exception of root and stalk
lodged plants percent and grain number per row. Phenotypic variances followed the
same tendency. The obtained values of broad-sense heritability were significantly
higher in S1 than in HS progenies. Low heritability values were calculated only for
root and stalk lodged plants percent. Cluster analysis did not show any greater difference between S1 and HS progenies, neither in distances nor in group definition, although there were significant differences between them in genetic variances. However, one can see a lower degree of distances between HS progenies.
36
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OSETLJIVOST KOMERCIJALNIH ZP SAMOOPLODNIH
LINIJA NA POJEDINE SULFONILUREA HERBICIDE
Vesna Dragi~evi}1, Milena Simi}1, Milan Brankov2, Igor Spasojevi}1,
Mile Se~anski1, Anika Ni{avi}1 i Branka Kresovi}1
2Stipendista
1Institut za kukuruz “Zemun Polje”, Beograd
Ministarstva za nauku i tehnolo{ki razvoj Republike Srbije
Linije kukuruza su osetljive na visoku zakorovljenost, kao i na upotrebu herbicida.
Ogled je postavljen sa ciljem da se ispita osetljivost razli~itih komercijalnih ZP linija
pri primeni herbicida nikosulfurona (preparat Motivell) i foramsulfurona (preparat
Equip) tokom 2006-2008. Tokom 2006-2008, o{te}enja koja su se ogledala u
pove}anim EWRC vrednostima bila su u ve}oj meri prisutna kod Equip-a, u odnosu
na Motivell. U tretmanima sa herbicidima sadr`aj suve materije je bio ve}i tokom
su{nih sezona, dok je bio manji tokom vla`nih sezona, u pore|enju sa kontrolom.
Sli~an trend je bio prime}en i na nivou rastvorljivih proteina: herbicidi su pove}ali
njihov sadr`aj tokom su{nih sezona, a smanjili ga tokom vla`nih sezona. Promene u
sadr`aju fenola, kao va`nih antioksidanata su varirale po godinama i tretmanima.
Mo`e se ista}i da je Equip doveo do zna~ajnog smanjenja njihove koncentracije, dok
je Motivell doveo do pove}anja sadr`aja, u odnosu na kontrolu. Moglo bi se zaklju~iti
da je nikosulfuron bezopasniji za upotrebu u semenskom usevu, u odnosu na
foramsulfuron, mada je odabir odgovaraju}eg herbicida diktiran meteorolo{kim
faktorima.
SUSCEPTIBILITY OF COMMERCIAL ZP INBRED
LINES TO SOME SULFONYLUREA HERBICIDES
Maize inbreds are susceptible to high weed infestation and the use of herbicides. The
trial was set up to determine susceptibility of various commercial ZP inbreds to
nicosulphuron (preparation Motivell) and foramsulphuron (preparation Equip) herbi cides dur ing 2006-2009. Dur ing 2006-2008 higher dam ages, pres ent in higher
EWRC values were induced by Equip in relation to Motivell. Dry matter content in
herbicide treatments was higher during dry seasons, while it was lower during seasons
with higher precipitation, in comparison to control. Similar trend was observed at
level of soluble proteins: herbicides induced increase of their content during dry seasons and decrease during years with higher precipitation. The change of phenolics, as
important antioxidants varied upon years and treatments. It could be underlined that
Eqip gen er ally in duced sig nif i cant de crease of phenolics’ con cen tra tion, while
Motivell induced their decrease, in comparison to control. It could be concluded that
nicosulphuron is safer for ap pli ca tion in maize seed crop, com pared to
foramslphuron, while the selection of proper herbicide may be directed by meteorological conditions.
37
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POSTRNI USEVI KUKURUZA KAO POTENCIJALNA
SIROVINA ZA BIOGORIVO
Milomir Filipovi}1, Sne`ana Mladenovi} Drini}1, Milena Simi}1,
Zoran ^amd`ija1, Milan Stevanovi}1 i Bojan Stipe{evi}2
1Institut za kukuruz „Zemun Polje, Beograd
2Poljoprivredni fakultet Osijek, Hrvatska
Postrni usevi se tradicionalno koriste za hranu, sila`u, zeleno |ubrivo i od nedavno za
proizvodnju biomase za energiju. Cilj ovog rada je biolo ispitivanje postrnog useva
ZP hibrida kukuruza za proizvodnju biomase koja }e se dalje korisititi kao
ligocelulozna sirovina za proizvodnju etanola. ^etiri rana hibrida ZP161, ZP172,
ZP173, ZP196 su posejana po~etkom jula nakon p{enice. Hibrid ZP172 imao je
najve}u ukupnu suvu masu i suvu biomasu dok je hibrid ZP161 imao najmanju
ukupnu suvu materiju a hibrid ZP196 suvu biomasu. Odnos zrna u ukupnoj suvoj
masi se razlikovao izme|u hibrida. Najni`i je imao hibrid ZP172 a najve}i hibrid
ZP196. Hibrid ZP173 je imao najvi{i `etveni indeks (80,1%) a hibrid ZP172 najni`i,
0,623 (62,3%). Prinos zrna je varirao od 4,49t/ha (hibrid ZP196) do 6,59t/ha (ZP172).
Najvi{i sadr`aj celuloze je imao hibrid ZP161 anajve}i sadr`aj hemiceluloze hibrid
ZP172. Hibrid ZP172 kao postni usev mo`e da se koristi za proizvodnju zrna i
biomase.
Klju~ne re~i: biomasa, kukuruz, etanol, postrni usev
DDOUBLE CROPS ZP MAIZE HYBRIDS
AS RAW MATERIAL FOR BIOFUEL
The second crops, planted mostly in the summer after winter crops are traditionally
used for food, forage (grain, silage), green manuring, and more recently use to produce a biomass for energy. The aim of this study was to evaluate ZP maize hybrids as
double crop, for production of biomass that further could be use as lignocelluloses
raw material for ethanol production. The four early maturing hybrids ZP161, ZP172,
ZP173, ZP196 was planted after winter wheat on beginning of July. The hybrid ZP172
has the highest total and biomass dry matter, while hybrid ZP161 has the lowest total
dry matter, as well hybrid ZP196 has the lowest biomass dry matter. The proportion
of grain in total dry matter differs across hybrids. The lowest has hybrid ZP172 and
the highest hybrid ZP196. The hybrid ZP173 has the highest harvest index (80,1%)
and hybrid ZP172 the lowest one, 0,623 (62,3%). The grain yield varied from 4,49t/ha
(hybrid ZP196) to 6,59t/ha (ZP172). The highest cellulose content has hybrid ZP161
and the highest hemicelluloses content has hybrid ZP172. The hybrid ZP172 showed
the potential for grain and biomass production under double-cropping conditions.
38
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ STENICOM (Eurygaster austriaca Schrk i E. maura)
O[TE]ENIH ZRNA NA KVALITET P[ENICE
Veselinka \uri}, Novica Mladenov, Nikola Hristov,
Ankica Kondi}-[pika, Vladimir A}in i Lazar ^obanovi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Analiziran je uticaj uve}anog broja `itnih stenica iz `etve 2005 god. na kvalitet 5
NS-sorti p{enice sa 10 lokaliteta u Vojvodini. Dobijeni rezultati za sadr`aj proteina
(%), sadr`aj va`nog glutena (%) i sedimentacionu vrednost (ml) po Zeleny testu nisu
odgovarali pravom stanju tehnol{kog kvaliteta stenicom o{te}enih zrna i bra{na.
Prisustvo stenicom o{te}enih zrna je registrovano modifikovanim Zeleny testom.
Problemi zbog stenicom o{te}enih zrna se javljaju tek u fazi odmaranja testa od
p{eni~nog bra{na, zna~ajnim smanjenjem energije testa (cm 2) na ekstenzografu.
Uticaj steni~avih zrna na energiju testa zavisio je od broja stenicom nagri`enih zrna i
sorte.
Seme p{enice o{te}eno napadom `itne stenice u setvi 2004. nije uticalo na pogor{anje
tehnolo{kog kvaliteta p{enice roda 2005. godine.
EFFECT OF WHEAT BUG (Eurygaster integriceps and E. maura)
DAMAGE ON WHEAT GRAIN QUALITY
We assessed the effect of increased numbers of wheat bugs during the 2005 wheat harvest on quality of five NS wheat cultivars grow in 10 locations in the Vojvodina Province. The results for protein content (%), wet gluten (%) and sedimentation value
(ml) according to the Zeleny test did not correspond to the true state of technological
quality of the grain and flour damaged by the pest. The presence of damaged grains
was registered with a modified Zeleny test. Damaged grains caused problems during
the rest ing phase of dough, signif i cantly reduc ing the dough en ergy (cm 2 ) on
Brabender extensograph. The impact of damaged grains on dough energy depended
on the number of damaged grains and cultivar.
Wheat seed damaged by the wheat bug attack during the 2004 sowing did not affect
the technological quality of wheat grain from the 2005 harvest.
39
Sekcija / Section 2
Oplemenjivanje industrijskog bilja
Breeding of industrial crops
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ISKORI[]AVANJE SELECTIVE SWEEP-A U
OPLEMENJIVANJU SOJE: TEORIJSKE POSTAVKE
Vuk \or|evi}, Jegor Miladinovi}, Svetlana Bale{evi}-Tubi},
Vojin \uki}, Aleksandar Miki} i Kristina Petrovi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Selective sweep je pojava smanjivanja varijabilnosti selekciono neutralnih alela kada
se nalaze u okolini gena koji je pod jakim uticajem selekcije. Istorijski posmatrano,
oplemenjivanje soje zapo~elo je sa malim brojem genotipova i mo`e se posmatrati
kao rekurentna selekcija na prinos. Ako se posmatraju frekvencija alela u preda~koj i
u populaciji elitnih linija soje koje se gaje u odre|enom geografskom regionu, mogu}e
je uo~iti selekciono neutralne alele koji su zna~ajno promenili frekvenciju. Svaki alel
koji ima razli~itu frekvenciju u preda~koj i elitnoj populaciji ukazuje na delovanje
selekcije u okolini datog lokusa. Na osnovu ispitivanja neutralne varijabilnosti u
preda~koj i elitnoj populaciji, mogu}e je prona}i mesta u genomu soje koja su pod
uticajem selekcije, odnoso koja uti~u na pove}anje prinosa. Ovi delovi genoma su
vezani sa agronomski bitnim genima i mogu}e ih je iskoristit u marker asistiranoj
selekciji. Primenom analize promena frekvencija i selective sweep-a, mogu}a je
identifikacija agronomski bitnih delova genoma soje, koji igraju zna~ajnu ulogu u
pove}anju prinosa za odabrani set elitnih linija.
Klju~ne re~i: soja, selective sweep, preda~ki genotipovi, elitni genotipovi
EXPLOITING SELECTIVE SWEEP IN SOYBEAN
BREEDING: THEORETICAL ASSUMPTIONS
Selective sweep is phenomenon of reduction of neutral variability in the vicinity of
genes that is strongly influenced by selection. Historically, soybean breeding began
with a small number of genotypes and can be seen as a recurrent selection for yield.
Analyzing allele frequencies in ancestral and elite population for particular region, it
is possible to find neutral alleles with significant different frequencies between two
populations. Each allele, which has a different frequency in the elite and ancestral
population, indicates selection effects in the vicinity of a given locus. Exploring neutral variability in ancestral and elite population, it is possible to find soybean genomic
locations which are under selection, actually affecting the yield increase. These parts
of the genome are associated with agronomically important genes and can be used in
marker assisted selection. Applying the analysis of frequency changes and selective
sweep, it is possible to identify agronomically important parts of soybean genome,
which play an important role in yield increase for the selected set of elite lines.
Key words: soybean, selective sweep, ancestral genotypes, elite genotypes
42
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ISKORI[]AVANJE SELECTIVE SWEEP-A U
OPLEMENJIVANJU SOJE: PRIMENA U OPLEMENJIVANJU
Vuk \or|evi}, Jegor Miladinovi}, Svetlana Bale{evi}-Tubi},
Vojin \uki}, Aleksandar Miki} i Kristina Petrovi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Za analizu selective sweep-a kod soje kori{}ene su dve populacije (12 preda~kih i 15
elitnih genotipova soje) i tri neutralna lokusa (T, W1 i Rpm). Hi-kvadrat testom
ustanovljene su promene frekvencije alela na lokusima T i W1. O~ekivane frekvencije
izra~unate se na osnovu pedigrea i odabrani preda~ki genotipovi ~ine oko 80%
geneti~ke osnove elitnih genotipova. Kada se primeni deterministi~ki model selective
sweep-a, r/s odnos za T lokus iznosi 0.007, dok za W1 0.074 (r-u~estalost
rekombinacija izme|u neutralnog i selektivnog lokusa, s-efekat gena). Pod
pretpostavkom sli~ne u~estalosti rekombinacija oko ova dva lokusa, efekat gena na
pove}anje prinosa je zna~ajno ve}i za T lokus. Radi provere, obavljeno je
pretra`ivanje QTL-ova u Soybase. U okolini od 2cM od W1 lokusa nisu detektovani
QTL-ovi koji uti~u na prinos, dok se u okolini T lokusa nalaze dva. Kada je pro{iren
interval na 10 cM, oko W1 konstatovana su 2 QTL-a za prinos, dok je u okolini T
prona|eno ~ak 15 QTL-ova. Pored toga, okolina T lokusa je bogata i QTL-ovima za
druge agronomski va`ne osobine. Primenom analize alelnih frekvencija i selective
sweep-a, mogu}e je odrediti lokaciju i efekat gena koji uti~u na pove}anje prinosa.
Klju~ne re~i: soja, selective sweep, r/s vrednost, QTL
EXPLOITING SELECTIVE SWEEP IN SOYBEAN
BREEDING: APPLICATION IN BREEDING
Selective sweep analysis was used in two soybean populations (12 ancestral and 15
elite soybean genotypes) and three neutral loci (T, W1 and Rpm). Chi-square test indicates significant allele frequencies changes at T and W1 loci. Expected frequencies
are calculated based on pedigree data and ancestral genotypes represent about 80%
of the genetic base of elite genotypes. When applied a deterministic model of selective sweep, the r/s ratio for the T locus is 0.007 and 0.074 for W1 (r-recombination frequency between neutral and selective loci, s-gene effect). Assuming similar recombination frequencies around the two loci, the gene effect of yield increase was significantly greater for the T locus. For verification, search for QTLs was conducted at
Soybase. In the interval of 2 cM around the W1 locus were not detected QTLs affecting yield, while in the same interval of T locus, two QTLs was found. When the interval was extended to 10 cM, two QTLs were found around W1 locus, while around T
locus 15 yield QTLs was found. In addition, the genomic region around T locus is rich
in QTLs for other agronomically important traits. Using frequency analysis and selective sweep, it is possible to determine the location and effect of genes that increase
yield.
Key words: soybean, selective sweep, r/s ration, QTL
43
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ISPITIVANJE NA^IN DELOVANJA GENA I PROPORCIONALNOG
DOPRINOSA RODITELJSKIH GENOTIPOVA U NASLEDJIVANJU
PRINOSA KORENA I [email protected] [E]ERA KOD DIPLOIDNIH HIBRIDA
[E]ERNE REPE
Ivica Stan~i}, Sa{a Petrovi} i Jelica @ivi}
Visoka poljoprivredno-prehrambena {kola, Prokuplje
U radu je analiziran na~in delovanja gena i proporcionalni doprinos roditeljskih
genotipova u nasle|ivanju prinosa korena i sadr`aja {e}era kod diploidnih hibrida
{e}erne repe. Za ova istra`ivanja kori{}ene su dve diploidne monogermne mu{ko
sterilne linije i tri jednostruka (SC) mu{ko sterilna hibrida kao maj~inske
komponente, a kao opra{iva~i kori{}ena su tri multigermna diploida. Ra{~lanjivanje
genotipske varijanse na aditivnu i dominantnu komponentu izvr{eno je preko
kovarijansi polusrodnika (HS) i punih srodnika (FS). Prikazan je proporcionalni
doprinos pojedinih komponenata ukr{tanja (linija, testera i interakcija) u ekspresiji
odre|ene osobine F1 generacije. Utvr|eno je da komponente genotipske varijanse
pokazuju zna~ajan uticaj neaditivnog delovanja gena (dominacije) u nasle|ivanju
prinosa korena i sadr`aja {e}era, dok je aditivno dejstvo gena manje zna~ajno. Ve}i
proporcionalin doprinos ekspresiji prinosa korena imale su maj~inske komponente,
dok je kod sadr`aja {e}era ustanovljen podjednak doprinos linija, opra{iva~a i njihove
interakcije.
Klju~ne re~i: {e}erna repa, prinosa korena, sadr`aj {e}era, genotipska varijansa
TESTING MODE OF ACTION OF GENES AND CONTRIBUTIONS
PROPORTIONAL PARENTAL GENOTYPES IN THE INHERITANCE
OF ROOT YIELD AND SUGAR CONTENT IN DIPLOID HYBRIDS OF
SUGAR BEETT
The paper analyzes the mode of action of genes and the proportional contribution of
inheritance of parental genotypes in root yield and sugar content in diploid hybrids of
sugar beet. For this study used two male sterile diploid monogerm lines and three single (SC) male sterile hybrids as maternal components, and are used as pollinators
multigerm three diploids. The breakdown of genotypic variance to additive and dominant component of covariance was performed by half-sib (HS) and full of relatives
(FS). Shows the proportional contribution of individual components of intersection
(of lines, testers and interactions) in the expression of certain characteristics of F1
generation. It was found that the components of genotypic variance showed a significant effect non-additive gene action (dominance) in the inheritance of root yield and
sugar content, while the additive effect of genes significantly less. Greater contribution to expression proportional root yield had a maternal component, while the sugar
content in established lines of equal contribution, pollinators and their interactions.
Key words: sugar beet, root yield, sugar content, genotypic variance
44
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NA^IN NASLE\IVANJA I KOMBINACIONE SPOSOBNOSTI ZA VISINU
BILJKE I PRE^NIK GLAVE SUNCOKRETA (Helianthus annuus L.)
Nada Hladni1, Vladimir Mikli~1, Sini{a Joci}1,
Marija Kraljevi}-Balali}2 i Dragan [kori}3
3Srpska
1Institut za ratarstvo i povrtarstvo, Novi
2Poljoprivredni fakultet, Novi Sad
Sad
akademija nauka i umetnosti, Ogranak u Novom Sadu, Novi Sad
Stvaranje novih hibrida suncokreta visokog genetskog potencijala za prinos semena i
ulja zahteva posedovanje informacije o na~inu nasle|ivanja i kombinacionim
sposobnostima visine biljke i pre~nika glave u F1 generaciji. Za ova istra`ivanja
kori{}eno je trinaest novih divergentnih (A) citoplazmatski mu{ko sterilnih inbred
linija, tri Rf-restorer linije kao testeri i njihovi F1 hibridi. Izme|u ispitivanih A-linija,
Rf-tester linija i njihovih F1 hibrida ustanovljene su zna~ajne razlike u visini biljke i
pre~niku glave. Na~in nasle|ivanja visine biljke bio je superdominacija roditelja ve}e
srednje vrednosti, a pre~nika glave dominacija i superdominacija roditelja ve}e
srednje vrednosti. Visoko zna~ajne pozitivne vrednosti OKS za oba svojstva pokazale
su cms A-linija NS-G-7 i Rf tester linija RHA-N-49. Najve}u visoko zna~ajnu
pozitivnu vrednost PKS za visinu biljke ispoljio je hibrid NS-G-1xRHA-N-49, a za
pre~nik glave hibrid NS-G-8xRUS-Rf-ol-168. Neaditivna komponenta genetske
varijanse imala je glavnu ulogu u nasle|ivanju oba ispitivana svojstva {to potvr|uje
odnos OKS/PKS u F1 generaciji koji je manji od jedinice. Najve}i prose~an doprinos u
ekspresiji visine biljke i pre~nika glave imale su A linije majke.
Klju~ne re~i: suncokret, visina biljke, pre~nik glave, kombinacione sposobnosti,
efekat gena
MODE OF INHERITANCE AND COMBINING ABILITY FOR PLANT
HEIGHT AND HEAD DIAMETER IN SUNFLOWER (Helianthus annuus L.)
The development of sunflower hybrids with high genetic potential for seed and oil
yields requires information on the GCA and SCA for plant height and head diameter
in the F1 generation. Used in this study were thirteen new divergent cms inbred lines
(A), three Rf restorers utilized as testers, and their F1 hybrids. The inbred A-lines,
Rf-testers and F1 hybrids differed significantly in the mean values of plant height and
head diameter. The mode of inheritance for plant height was superdominance of the
better parent and for head diameter it was dominance and superdominance of the
better parent. Highly significant positive values of GCA for both traits were found in
cms inbred line NS-G-7 and restorer line RHA-N-49. The greatest highly significant
pos i tive SCA value was found in NS-G-1xRHA-N-49 for plant height and in
NS-G-8xRUS-Rf-ol-168 for head diameter. Non-additive genetic variance played the
main role in the inheritance of both traits as confirmed by the GCA/SCA ratios in the
F1 generation, which were invariably smaller than unit. The female A lines had the
highest contribution to the expression of head diameter and plant height
Key words: sunflower, plant height, head diameter, combining ability, gene effect
45
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ANALIZA PROMENLJIVOSTI NEKIH KVANTITATIVNIH SVOJSTAVA
KOD SUNCOKRETA (H. annuus L.) PRIMENOM TTC METODE
Radovan Marinkovi}, Milan Jockovi}, Ana Marjanovi} Jeromela,
Jovanka Atlagi}, Velimir Radi} i Petar ^anak
Institut za ratarstvo i povrtarstvo, Novi Sad
Prou~avana je promenljivost prinosa semena, visine biljaka i pre~nika glave u
populaciji slu~ajnog ukr{tanja primenom TTC metode. TTC je eksperimentalni
dizajn za otkrivanje nealelne interakcije i ocene genetskih komponenata varijanse za
kvantitativna svojstva. Dve in bred linije su prvo ukr{tane izme|u sebe da bi se
proizvela njihova F1 generacija. Svaki od ova tri genotipa (kori{teni kao testeri)
ukr{teni su slede}e godine sa deset razli~itih linija i proizvedeno je 30 ukr{tanja.
Visoko zna~ajne razlike izme|u hibrida,tretmana, roditelja i linija konstatovane su za
visinu biljke, a visoko zna~ajne razlike izme|u hibrida, roditelja i linija za prinos
semena i pre~nik glave. Za pre~nik glave konstatovano je postojanje i visoko
zna~ajnih razlika izme|u testera. Ocene genetskih komponenti varijanse ura|ene su
na osnovu suma i razlika. Aditivna komponenta bila je zna~ajno ve}a od dominantne
za sva tri ispitivana svojstva. Prose~an stepen dominacije bio je manji od jedinice i
ukazuje da se u nasle|ivanju ispitivanih svijstava ispoljila parcijalna dominacija.
Klju~ne re~i: dominacija, aditivnost, analiza varijanse, eksperiment III
ANALIYSIS OF VARIABILITY OF SOME QUANTITATIVE TRAITS IN
SUNFLOWER (H. annuus L.) USING THE TTC METHOD
Variability of seed yield, plant height and head diameter in population of random
crosses using the TTC method was studied. TTC is experimental design for discovering non-allelic interaction and evaluation of genetic components of variance for quantitative traits. Two inbred lines were firstly crossed between themselves to obtain
their F1 generation. Every one of these three genotypes (used as testers) were crossed
next year with ten different lines and 30 crosses were produced.
Highly significant differences between hybrids, treatments, parents and lines were
concluded for plant height, while highly significant between hybrids, parents and lines
for seed yield and head diameter. For head diameter was concluded the existence of
highly significant differences between testers. Evaluation of genetic components of
variance were conducted on the basis of the sum and differences. Additive component was significantly higher than dominant for all three studied traits. The average
degree of dominance was less than unity and indicates that the inheritance of the
studied traits exhibited partial dominance.
Key words: domination, additivity, analysis of variance, experiment III
46
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
TESTIRANJE INBRED LINIJA SUNCOKRETA NA TOLERANTNOST
PREMA CRNOJ PEGAVOSTI STABLA SUNCOKRETA
Bo{ko Dedi}, Sonja Tan~i}, Jelena Mr|a, Sreten Terzi}, Sini{a Joci} i Vladimir Mikli~
Institut za ratarstvo i povrtarstvo, Novi Sad
Phoma macdonaldii Boerema, prouzrokova~ crne pegavosti stabla suncokreta je
uobi~ajen patogen suncokreta {irom sveta. Razvoj gljive prouzrokuje nekrozu tkiva
doma}ina {to se manifestuje defolijacijom i ranim sazrevanjem suncokreta. Potpuna
otpornost prema ovoj bolesti nije poznata, a dokazan je razli~it nivo osetljivosti
ispitivanih genotipova. Testiranje 70 in bred linija suncokreta je sprovedeno na
eksperimentalnom polju Rimski [an~evi. Biljke suncokreta su ve{ta~ki inokulisane
micelijom gljive u fazi butonizacije. Nakon inokulacije merena je du`ina lezija na
stablu u vremenskom razmaku od 10 dana od po~etka merenja, a podaci su upore|eni
putem analize varijanse. Zabele`ene su zna~ajne razlike u tolerantnost izme|u
testiranih linija prema crnoj pegavosti stabla suncokreta.
Klju~ne re~i: suncokret, Phoma macdonaldii, tolerantnost
TESTING A VARIETY OF SUNFLOWER INBRED
LINES FOR TOLERANCE TO PHOMA BLACK-STEM
Phoma macdonaldii Boerema causal agent of Phoma black-stem is a pathogen commonly found in sunflower crop all over the world. The fungus growth is causing necrosis of host plant tissues resulting in early defoliation and premature ripening. Complete resistance of sunflower to this disease is unknown, but there are reports of difference in response of different genotypes. Testing of 70 inbred lines was conducted
in Rimski [an~evi experimental field. Sunflower plants were artificially inoculated
with mycelial plugs in budding stage. Length of stem lesions was measured every 10
days, after start of measurement, and compared using analysis of variance. Significant
differences in response of tested lines to phoma black-stem were found.
Key words: sunflower, Phoma macdonaldii, tolerance
47
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ SESTRINSKOG UKR[TANJA NA PRINOS ZRNA SOJE
Mirjana Srebri}, Vesna Peri}, Dragan Kova~evi} i Sne`ana Mladenovi} Drini}
Institut za kukuruz „Zemun Polje”, Beograd
Soja spada u samooplodne biljne vrste. Varijabilnost svetskog sortimenta soje nije
velika kao kod nekih drugih biljnih vrsta. Metod sestrinskog ukr{tanja nije ~esto
kori{}en u selekcionim programima, posebo ako se ukr{tanje radi ru~no. Ovu metodu
selekcioneri koriste u cilju da stvore bolju koncentraciju po`eljnih alela. Kao po~etni
roditelji za formiranje matreijala u ovom radu kori{}ene su adaptirana
visokoprinosna sorta Kador i sorta Kunitz, bez Kunitz tripsin inhibitora u zrnu. Za
sestrinsko ukr{tanje su odabrane dve F 3 linije sa razli~itim sadr`ajem tripsin
inhbitora. Potomstva biljaka koje su kori{}ene kao majke i potomstva sestrinskih
ukr{tanja su testirana u polju. Prisustvo Kunitz tripsin inhibitora je utvr|eno
postupkm gelelekzroforeze. Linije dobijene iz sestrinskih ukr{tanja su uglanom bile
prinosnije od linija dobijenih od majki. Potomstva sestrinskih ukr{tanja su u proseku
pokazala signifikantno vi{i prinos u odnosu na potomstva majki (11.29 g i 12.18g po
biljci). Identifikovano je ~etiri linije prinosnije od boljeg po~etnog roditelja, sorte
Kador. Jedna od njih ne sadr`i Kunitz tripsin inhibitor.
Klju~ne re~i: soja, sestrinska ukr{tanja, Kunitz tripsin inhibitor
EFFECT OF SISTER CROSSING TO SOYBEAN GRAIN YIELD
Soybean is self pollinated plant. Variability between grown soybean varieties around
the world is not large, like in some other plants. Sister cross method is not often used
in breeding programs, especially if crossing is handmade. This method was prevalently used by breeders in effort to concentrate desirable alleles. Adapted high yielding soybean variety Kador and Kunitz-trypsin inhibitor free variety Kunitz were
crossed as initial parents. Two F3 lines with different content of KTI were chosen to
made sister crossing. Progenies of mother’s plants and their sister crosses were tested
in field trails. Presence of Kunitz trypsin in hib i tor was deter mined us ing
gelelectrophoresis. Lines derived from sister cross were mostly high yielding in addition to their mother’s progenies lines. Sister crossing progenies had significantly
higher yield than mother’s progenies, in average (11.29 g and 12.18 g per plant respectively). There were found four lines with highest yield from better initial parent
Kador. One of them is Kunitz-free.
Key words: soybean, sister crosses, Kunitz trypsin inhibitor
48
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENOTIPSKE SPECIFI^NOSTI SOJE - Glycine max (L.) MERR.
U USLOVIMA FOLIJARNE ISHRANE
Vera Popovi}1, Milo{ Vidi}1, Miroslav Male{evi}1, \or|e Glamo~lija2,
Sne`ana Jak{i}1, Jela Ikanovi}2 i Miladin Kosti}1
1Institut za ratarstvo i povrtarstvo, Novi
2Poljoprivredni fakultet, Zemun
Sad
U radu je prou~en uticaj folijarne ishrane, kompleksnim te~nim |ubrivom, sa makroelementima (N, P i K-12:4:6) i mikroelementima (Fe, Mn, Cu, B, Zn) na prinos i
hemijski sastav zrna, kod NS sorti soje, razli~ite grupe zrenja. Prose~na masa 1000
zrna, bila je statisti~ki zna~ajno vi{a u 2010. (161.2 gr) u odnosu na 2009. (132.38 g).
Najmanju masu 1000 zrna u proseku imala je sorta Galina (140 g). najve}i sadr`aj
proteina u kontrolnoj varijanti imala je sorta Victoria dok je najve}i sadr`aj ulja
zabele`en kod sorte Tea. U varijanti sa folijarnom ishranom kod sorte Tea utvr|en je
statisti~ki zna~ajno vi{i sadr`aj proteina i ulja na u odnosu na ostale testirane sorte.
Sadr`aj ulja bio je, kako u kontrolnoj tako i u varijanti sa folijarnom ishranom, u
nega tivnoj statisti~ki zna~ajnoj korelaciji sa masom 1000 zrna (-0.86, -0.80) i u
negativnoj korelaciji sa sadr`ajem proteina u zrnu soje (-0.42, -0.04). Prinos je, kako u
kontrolnoj varijanti tako i varijanti sa folijarnom ishranom, bio u negativnoj korelaciji
sa sadr`ajem proteina (-0.36, -0.05). U varijanti sa folijarnom ishranom prinos je bio u
pozitivnoj korelaciji sa sadr`ajem ulja (0.21) i pozitivnoj statisti~ki zna~ajnoj
korelaciji sa sadr`ajem ulja u kontrolnoj varijanti (0.53).
Key words: Glycine max (L.) Merr., prinos, masa 1000 zrna, sadr`aj proteina i ulja,
folijarna ishrana
GENOTYPIC SPECIFICITY OF SOYBEAN [Glycine max (L) MERR.]
UNDER CONDITIONS OF FOLIAR FERTILIZATION
We assessed the impact of foliar fertilization with a complex liquid fertilizer that combined macroelements (N, P, K - 12:4:6, respectively) and microelements (Fe, Mn, Cu,
B, Zn) on yield and grain chemical compositions of NS soybean cultivars of different
maturity groups. The average weight of 1000 grains was significantly higher in 2010
(161.2 g) than in 2009 (132.38 g). The lowest average 1000-grain weight was registered
for the cultivar Galina (140 g). The cultivar Victoria had the highest protein content is
the control variant, the cultivar Tea had the highest oil content (21.73 %). In the variant of foliar fertilization, the cultivar Tea had significantly higher contents of proteins
and oil (37.5 % and 21.58 %, respectively) than the other tested varieties.
The oil content was significantly and negatively correlated with 1000-grain weight
(-0.86, -0.80) and negatively corelated with protein content in soybean (-0.42, -0.04) in
both, the control variant and the variant with foliar fertilization. The yield was negatively correlated with protein content (-0.36, -0.05) in the control variant and the variant with foliar fertilization. The yield in the variant with foliar fertilization was positively correlated with oil content (0.21) and significantly positively correlated with oil
content (0.53) in the control variant.
Key words: Glycine max (L) Merr., yield, 1000-grain weight, protein and oil content,
foliar fertilization
49
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETSKI NAPREDAK SOJE POTPOMOGNUT
MOLEKULARNIM MARKERIMA
Aleksandra Sudari}, Marija Vratari} i Maja Mato{a
Poljoprivredni institut Osijek, Osijek, Hrvatska
Genetski napredak soje, Glycine max (L.) Merr., kao rezultat kontinuiranog oplemenjivanja i selekcije ima zna~ajnu ulogu u pove}anju njene proizvodnje i kvalitete kao i
u unapre|enju prehrambene i funkcionalne kvalitete zrna. Iznos genetske dobiti u
kvan titeti i kvaliteti zrna soje zna~ajno ovisi o genetskoj razli~itosti odabranih
roditeljskih komponenti. Iz tog razloga, procjena genetske divergentnosti raspolo`ive
germplazme je presudni aspekt oplemenjivanja soje za maksimiranje genetskog
napretka. Cilj ovog istra`ivanja bio je procjeniti genetski progres u urodu zrna te
usporediti uspje{nost oplemenjiva~ke procedure sa i bez primjene molekularnih
markera (SSRs) u selekciji roditelja. Rezultati su ukazali na postignuti genetski
napredak u urodu. Genetska dobit bila je ve}a u oplemenjiva~koj proceduri s
primjenom molekularnih markera u identifikaciji sli~nosti/razli~itosti germplazme
soje. Kreiranje poljskih pokusa za identifikaciju kvalitetnih linija temeljeno na
informacijama dobivenim primjenom molekularnih markera je u~inkovitije,
produktivnije i ekonomi~nije.
Klju~ne rije~i: soja, urod, genetska dobit, SSRs, selekcija roditelja
GENETIC IMPROVEMENT IN SOYBEAN
AIDED BY MOLECULAR MARKERS
Genetic improvement in soybean, Glycine max (L.) Merr., as result of continual breeding and selection, has had impact role in its productivity and quality increasing as well
as improving nutritional and functional quality of the seed. The rate of genetic gain in
the quantity and quality of soybean grain depends considerably on the genetic diversity of the selected parental components. Hence, the assessment of the genetic diversity of the available germplasm is a crucial aspect of soybean breeding for maximizing
genetic improvement. The objective of this study was to evaluate genetic progress in
yield and to compare the effectiveness of breeding procedure with and without the
molecular markers (SSRs) application in parental selection. The results indicated the
genetic advance in yield. There was a positive association between the rate of gain
and molecular marker application in the identification of soybean germplasm similarity/dissimilarity. Designing field experiments for identification of promising lines
based on prior information obtained by molecular markers tends to be more efficient,
more productive and less costly.
Key words: soybean, yield, genetic gain, SSRs, parental selection
50
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PRINOS I KOMPONENTE PRINOSA KOD DVE POPULACIJE SOJE
Vesna Peri}1, Mirjana Srebri}1, Sne`ana Mladenovi} Drini}1 i Aleksandar Miki}2
1Institut za kukuruz „Zemun Polje”, Beograd
2Institut za ratarstvo i povrtarstvo, Novi Sad
Predmet ovog istra`ivanja bio je da se ispita me|uzavisnost prinosa i komponenti prinosa kod soje i utvrde komponente ~ijom bi se selekcijom dao najve}i doprinos
pobolj{anju prinosa zrna. Ogledi su vo|eni u toku dve godine na oglednom polju
Instituta za kukuruz Zemun Polje. Prou~avane su dve polusrodni~ke populacije soje,
od kojih je svaka bila sa~injena od 10 linija F4 generacije. Posmatrane su slede}e
feontipske osobine: visina biljke, broj nodusa na glavnom stablu, broj mahuna na
biljci, broj zrna po biljci, masa hiljadu zrna i prinos po parceli. U cilju dobijanja
informacija o varijabilnosti ovih kvantitativnih osobina, izra~unati su selekcioni
parametri: geneti~ki i fenotipski koeficijenti varijacije i heritabilnost u sirem smislu.
Na osnovu rezultata prve posmatrane populacije, broj zrna po biljci pokazao se kao
najefikasniji kriterijum za selekciju na prinos. U drugoj populaciji, masa 1000 zrna je,
pored broja zrna po biljci, utvr|ena kao komponenta sa najja~im uticajem na prinos
zrna.
Klju~ne re~i: soja, prinos zrna, komponente prinosa, korelacije
A STUDY OF SEED YIELD AND YIELD COMPONENTS
IN TWO SOYBEAN POPULATIONS
Objective of this study was to examine the association between yield and yield components in soybean and determine the components whose selection would contribute
to the improvement of genetic yield potential. The trials were conducted during two
years on the fields of Maize Research Institute Zemun Polje. Study involved two soybean half-sib populations; each consisted of 10 F4 lines. They were evaluated for
phenotypic traits: plant height, number of nodes per plant, pod number per plant,
number of seeds per plant, 1000 seed weight and seed yield per plot. In order to obtain information about variability of each quantitative trait, selection parameters such
as genotypic and phenotypic coefficients of variation and broad sense heritability
were calculated. Association between traits was analysed based on genotypic and
phenotypic correlation coefficients. Obtained results suggested that in first population seed number per plant was determined as the most efficient selection criteria in
breeding for seed yield. In second population, seed number per plant and 1000 seed
mass revealed as the components with the highest influence on seed yield.
Key words: soybean, grain yield, yield components, correlations
51
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ZNA^AJ SAVREMENE SELEKCIJE ZA PROIZVODNJU [E]ERNE
REPE U AGROEKOLO[KIM USLOVIMA U REPUBLIKE SRBIJE
Stevan Radivojevi}1, Jasna Grbi}1, Rada Jevti}-Mu~ibabi}1 i Vlada Filipovi}2
1Institut za prehrambene tehnologije, Novi
2PDS Institut “Tami{”, Pan~evo
Sad
Na osnovu izvedenih sortnih mikroogleda, sa sortama razli~ite tolerantnosti, na
najva`nija oboljenja {e}erne repe, u toku 1998. i 2008. godine, utvr|eno je slede}e.
Odstupanja izme|u ispitivanih dvanaest sorti {e}erne repe, u toku 1998. godine, kod
najbitnijih pokazatelja, bila su veoma visoka. Utvr|ene razlike u ovoj godini, izme|u
prvorangirane sorte i poslednje po rangu iznose: za prinos korena 53,49 t/ha ili
428,00%, za sadr`aj {e}era 7,36% apsolutnih ili 97,00% relativnih, za sadr`aj
natrijuma 74,33 mmol/100oS ili 963,00% i za prinos kristalnog {e}era 7,790 t/ha ili
1450,00%. Me|utim, ostvareni rezultati u toku 2008. godine, za dvanaest sorti {e}erne
repe su na zna~ajno vi{em nivou, a odstupanja izme|u sorti, prvorangirana i poslednja
po rangu, su zna~ajno ni`a. Primera radi: odstupanje u prinosu korena iznosi 28,37
t/ha ili 28,00%, u sadr`aju {e}era 1,46% apsolutnih ili 9,43% relativnih, u sadr`aju
natrijuma 15,60 mmol/100oS ili 650,00% i za prinos kristalnog {e}era 6,009 t/ha ili
43,57%. Prema tome, napredak u selekciji {e}erne repe za poslednjih deset godina je
veoma o~igledan i ima ogroman zna~aj za proizvodnju {e}erne repe i {e}era u
Republici Srbiji.
Klju~ne re~i: selekcija, sorta, {e}erna repa, prinos korena, tehnolo{ki kvalitet
IMPORTANCE OF MODERN CULTIVAR SELECTION
FOR THE PRODUCTION OF SUGAR BEET IN AGROECOLOGICAL
CONDITIONS OF SERBIA
This study encompasses the results of microtrials conducted over period 1998-2008 on
sugar beet cultivars with different levels of tolerance to major beet diseases. Fluctuations in the most important quality attributes within the twelve sugar beet accessions
were markedly high in 1998. The maximal differences i.e. the differences between the
first and the last-ranked cultivar were: for taproot yield 53.49 t/ha or 428.00%, for
sugar con tent 7.36% ab so lute or 97.00% rel a tive, for so dium con tent 74.33
mmol/100oS or 963.00% and for granulated sugar yield 7.790 t/ha or 1450.00%. However, the results achieved in 2008 for the selected accessions were significantly better
which reflected to much lower divergency between the first and last-ranked cultivars.
The maximal divergency for taproot yield was 28.37 t/ha or 28.00%, for sugar content
1.46% absolute or 9.43% relative, in sodium content 15.60 mmol/100oS or 650.00%
and for granulated sugar yield 6.009 t/ha or 43.57%. These results are a good illustration for the progress made in the selection of sugar beet cultivars during one decade
which is of great importance for successful sugar beet cultivation and sugar production in Serbia.
52
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ANALIZA GENETI^KE DIVERGENTNOSTI RODITELJSKIH
KOMPONENTI [E]ERNE REPE, Beta vulgaris var. saccharifera
Jelica @ivi}1,Sa{a Petrovi}1, Ivica Stan~i}1 i Desimir Kne`evi}2
1Visoka
poljoprivredno-prehrambana {kola strukovnih studija, Prokuplje
2Poljoprivredni fakultet, Le{ak
Pojava i relativno brzo {irenje rizomanije ~iji je prouzrokova~ virus nekroti~nog `utila
nerava repe (BNYVV), jo{ jednom je uticala na kompletnu promenu geneti~ke
osnove hibridnih sorti {e}erne repe. Odabranih 14-opra{iva~a, tetraploidnog
multigermnog tipa (RH1 - Rh14 4n mm) i 6 maj~inskih linija, 2n mm MS (diploidne
monogermne, mu{ki sterilne linije): 2MS; 7MS, 5MS, 1MS, 3MS, 4MS su kori{}ene za
ispitivanje njihove reakcije na zemlji{tu inficiranom sa virusom rizomanije na
razli~itim lokalitetima. U ovom radu analiziran je uticaj rizomanije na varijabilnost
prinosa korena, sadr`aj {e}era i prinos kristalnog {e}era. Utvr|ene su veoma zna~ajne
razlike u vrednosti ispitivanih osobina roditeljskih komponenti {e}erne repe. Kod
osetljivih genotipova prinos korena i sadr`aj {e}era je veoma zna~ajno ni`i nego kod
tolerantnih genotipova {e}erne repe. Primenjen je hijerarhiski metod grupisanja i
dobijeni rezultati prikazani su na dendrogramu za najzna~ajnije osobine {e}erne repe.
Klju~ne re~i: rizomanija, prinos korena, sadr`aj {e}era, prinos kristalnog {e}era,
dendrogram
ANALYSIS OF GENETIC DIVERSITY OF PARENT COMPONENTS
OF SUGAR BEET, Beta vulgaris var. saccharifera
The appearance and relatively quickly spread of rhizomania which is causing beet necrotic yellow vein virus (BNYVV), has once again resulted in a complete change in
the ge netic ba sis of hy brid cultivars of sugar beet.The se lected 14-pollinators,
tetraploids multigerm type (Rh1- Rh14 4n MM) and 6 maternal lines, 2n mm MS
(diploid monogerm, male sterile lines with their sterile analog): 2MS; 7MS, 5MS,
1MS, 3MS, 4MS were used for investigation of their reaction to infested soil by
rhizomania in different location. In this study analyzed influence of rhizomania to
variability of root of yield,sugar content and yield of white sugar . Differences of values of root yield and sugar content within parent cultivars of sugar beet were established. In susceptible genotype yield of root and content of sugar was highly significant lower than in tolerant genotypes of sugar beet. Hierarchical clustering method
applied and the results are shown in dendrogram for most properties of sugar beet.
Key words: rhizomania, root yield, content of sugar, yield of white sugar, dendrogram
53
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETSKA SPECIFI^NOST MINERALNE ISHRANE [E]ERNE REPE
Branko Marinkovi}, Jovan Crnobarac, Goran Ja}imovi} i Dragana Latkovi}
Poljoprivredni fakultet, Departman za ratarstvo i povrtarstvo, Novi Sad
Na vi{egodi{njem stacionarnom ogledu, ispitivana je genetska specifi~nost mineralne
ishrane {e}erne repe. Ogled se nalazi u ~etvoropoljnom plodoredu gde se ispituje 20
varijanti |ubrenja NPK hranivima, a koli~ine su 50, 100 i 150 kgha-1 aktivne materije
svakog elementa. Ogled je postavljen na zemlji{tu tipa karbonatni ~ermozem.
Kod sorte Dana pove}anjem koli~ine fosfora sa 50 na 100 i 150 kgha-1 prinos korena
se pove}ao za 5,1 i 9,5%, procenat {e}era se smanjio za 0,05 i 0,39%, a prinos {e}era se
pove}ao za 70 i 80 kgha-1. Prinos {e}era se nije smanjivao sa pove}anjem koli~ine
kalijuma.
Kod sortnog hibrida NS-H-1R sa pove}anjem koli~ine fosfora sa 50 na 100 i 150
kgha-1 prinos korena se smanjivao za 1,0 i 1,4%, a sa pove}anjem koli~ine kalijuma
smanjenje je bilo 2,0 i 4,2%. Pove}anjem koli~ine kalijuma procenat {e}era je varirao
od -1,55 do +0,22%.
Sa pove}anjem koli~ine fosfora prinos {e}era se pove}ao za 1,5% i 1,0%. Pri
pove}anju koli~ine kalijuma prinos {e}era bio je manji za 5,8% i 2,0%.
Klju~ne re~i: genetska specifi~nost, |ubrenje, {e}erna repa
GENETIC SPECIFICITY OF MINERAL NUTRITION OF SUGAR BEET
In long term stationary field trial, examined the genetic specificity of mineral nutrition of sugar beet. The experiment is located in four field crop rotation where is
tested 20 variants of NPK fertilizer nutrients and quantities are 50, 100 and 150 kgha-1
of active ingredient of each element. The soil in the experimental plots is classified as
calcareous chernozem.
In cultivar Dana increasing the amount of phosphorus from 50 to 100 and 150 kgha-1
root yield increased by 5.1 and 9.5%, the percentage of sugar was reduced by 0.05 and
0.39%, and sugar yield increased by 70 and 80 kgha-1. The yield of sugar is not reduced with increasing amounts of potassium.
In varietal hybrid NS-H-1R with increasing amounts of phosphorus from 50 to 100
and 150 kgha-1 root yield decreased by 1.0 and 1.4%, and with increasing amounts of
potassium decrease was 2.0 and 4.2 %. Increasing amounts of potassium percentage
of sugar varied from -1.55 to +0.22%. With increasing amounts of phosphorus increased sugar yield by 1.5% and 1.0%. When increasing amounts of potassium sugar
yield was lower by 5.8%.
Key words: genetic specificity, fertilization, sugar beet
54
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OCENA KOMPONENATA GENETSKE VARIJANSE ZA NEKA
KVANTITATIVNA SVOJSTVA KOD ULJANE REPICE (Brassica napus L.)
Ana Marjanovi} Jeromela, Radovan Marinkovi}, Jovanka Atlagi},
Petar Mitrovi}, @eljko Milovac, Velimir Radi} i Sreten Terzi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Kod tri hibridne kombinacije nastale ukr{tanjem {est sorti ozime uljane repice
analiziran je uticaj gena sa aditivnim i dominantnim efektima, kao i njihove
interakcije u nasle|ivanju visine biljke i visine prve bo~ne grane. Povezanost izme|u
o~ekivanih srednjih vrednosti potomstava proverena je primenom metode scaling
testova, a procene genetskih efekata i na~ina nasle|ivanja ura|ene su po metodi Generation Mean Analysis. Aditivno dominantan model nije bio adekvatan za visinu
biljaka kod sva tri ukr{tanja, a za visinu prve bo~ne grane kod drugog i tre}eg
ukr{tanja. Neadekvatnost modela je ukazala da su u nasle|ivanju navedenih svojstava
u tim ukr{tanjima veliki zna~aj imali i epistati~ni genski efekti.
U nasle|ivanju visine biljke kod sva tri ukr{tanja i visine prve bo~ne grane kod
drugog i tre}eg ukr{tanja ispoljila se duplikatna epistaza. Me|utim, treba napomenuti
da se u nasle|ivanju visine biljke kod prvog i tre}eg ukr{tanja i u nasle|ivanju visine
prve bo~ne grane kod tre}eg ukr{tanja ispoljila duplikatna pozitivna epistaza izme|u
dominantnih gena. S druge strane, kod drugog ukr{tanja u nasle|ivanju oba svojstva
ispoljila se duplikatna negativna dominantna epistaza.
Klju~ne re~i: visina biljke, visina prve bo~ne grane, epistaza, aditivan i dominantan
efekat
EVALUATION OF COMPONENTS OF GENETIC VARIANCE FOR SOME
QUANTITATIVE TRAITS IN RAPESEED (Brassica napus L.)
Influence of genes with additive and dominant effects, as well as their interaction for
inheritance of plant height and first lateral branch height were analyzed in three hybrid combinations obtained by crossing six winter rapeseed cultivars. Relationships
between the expected average values in progenies were checked using the scaling
tests method, while gene effects and mode of inheritance were estimated using Generation Mean Analysis. Additive-dominant model was not adequate for plant height in all
three crosses and for first lateral branch height in the second and third cross. Model
inadequacy implied that epistatic gene effects were important for inheritance of analyzed traits in those crosses.
Duplicate epistasis was manifested for inheritance of plant height in all three crosses
and in second and third cross for first lateral branch height. However, it should be
noted that a duplicate positive epistasis manifested between dominant genes for inheritance of plant height in first and third cross, and for first lateral branch height in
the third cross. On the other hand, duplicate negative dominant epistasis was manifested in the second cross for inheritance of both traits.
Key words: plant height, first lateral branch height, epistasis, additive and dominant
effects
55
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ EKOLO[KIH FAKTORA NA KVALITET METLICE SIRKA
METLA[A [Sorghum bicolor (L.) MOENCH]
Vladimir Sikora1, Jano{ Berenji1 i Dragana Latkovi}2
1Institut za ratarstvo i povrtarstvo, Novi Sad
2Poljoprivredni fakultet, Departman za ratarstvo i povrtarstvo,
Novi Sad
Ispitivan je uticaj agroekolo{kih faktora (prose~na dnevna temperatura vazduha,
suma temperatura, suma padavina i prose~na dnevna vla`nost vazduha) tokom
vegetacije na komponente kvaliteta metlice (du`ina peteljki, broj peteljki i fino}a
peteljki) sirka metla{a. Poljski ogledi su izvo|eni tokom pet agrometeorolo{ki
razli~itih godina na lokalitetu Ba~ki Petrovac. Analizom su obuhva}ene tri doma}e
(Reform, Prima i Tan Sava), dve ma|arske (Dia i Szegedi 1023) i jedna ameri~ka
sorta (Deer 418) sirka metla{a.
Interakcija izme|u genotipova i spoljne sredine je visokosignifikantna za sve analizirane komponente kvaliteta.
Analizirani agroekolo{ki faktori nemaju zna~ajan uticaj na du`inu peteljki. Broj
peteljki po metlici se pove}ava u uslovima ve}e dnevne tem perature vazduha a
smanjuje sa pove}anjem vla`nosti vazduha. Fino}a peteljki je u pozitivnoj korelaciji
sa prose~nom dnevnom temperaturom vazduha i sumom temperatura tokom
vegetacije dok je u vla`nijim uslovima koeficijent fino}e ve}i, odnosno fino}a peteljki
manja.
Klju~ne re~i: ekolo{ki uslovi, komponente kvaliteta, sirak metla{
EFFECT OF ECOLOGICAL FACTORS ON BROOMCORN PANICLE
QUALITY [Sorghum bicolor (L.) MOENCH]
Influence of agroecological factors (average air temperature, sum of temperature,
sum of precipitation and air humidity during vegetation period) on broomcorn panicle quality components (fiber lenght, fiber number per panicle and fiber fineness) was
examined in field trials through five agrometeorologicaly different year on locality
Ba~ki Petrovac. Analysis include three serbian (Reform, Prima and Tan Sava), two
hungarian (Dia and Szegedi 1023) and one american broomcorn variety (Deer 418).
Genotype x environment interaction is significant for all analysed quality components.
Agroecological factors have no significant influence on fiber lenght. Fiber number
per panicle is increasing with air temperature and decreasing in conditions of higher
air humidity. Fiber fineness is in positive correlation with average air temperature
and sum of temperature during vegetation period. In more humid conditions fineness
coefficient is higher and fiber are less quality.
Key words: broomcorn, ecological conditions, quality components
56
Sekcija / Section 3
Oplemenjivanje krmnog bilja,
povræa i ostalih ratarskih biljnih vrsta
Breeding of forage crops,
vegetables and other field crops
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DREVNA DNK ZRNENIH MAHUNARKI KAO VREMEPLOV
U RANE DANE NJIHOVOG GAJENJA
Aleksandar Miki}1, Petr Smikal2, Aleksandar Medovi}3, @ivko Jovanovi}4,
Bojan Zlatkovi}5, Branko ]upina6 i Vuk \or|evi}1
1Institut za ratarstvo i povrtarstvo, Novi Sad
2Prirodno-matemati~ki fakultet, Departman za botaniku, Olomouc, ^e{ka
3Muzej Vojvodine, Novi Sad
4Institut za molekularnu genetiku i geneti~ko in`enjerstvo, Beograd
5Prirodno-matemati~ki fakultet, Departman za biologiju i ekologiju, Ni{
6Poljoprivredni fakultet, Departman za ratarstvo i povrtarstvo, Novi Sad
Zrnene mahunarke, poput gra{ka (Pisum sativum L.) i urova (Vicia ervilia (L.) Willd.),
smatraju se jednim od najstarijih domestikovanih biljaka, {to se najverovatnije desilo
u dana{njoj Siriji pre oko 10000 godina. Odatle, brzo su se ra{irile preko Male Azije i
preko Balkanu u{le u postglacijalnu Evropu. Nedavni pomaci u istra`ivanju humane
`ivotinjske drevne DNK izazvali su zanimanje za analizom izolovane DNK iz
ugljenisanih semena zrnenih mahunarki. Prvi poznati svetski uspeh ostvaren je izolacijom i validacijom drevne DNK iz ugljenisanih semena gra{ka i urova iz ranogvozdeno dopskog naselja Hisar kod Leskovca, starih oko 3200 godina. Istovremeno,
potvr|eni su i zastarelii navodi o postojanju divljeg gra{ka (Pisum sativum subsp.
elatius) u flori Srbije otkri}em nekoliko populacija u dolini reke P~inje. Prvi rezultati
uporedne analize drevne DNK sa Hisara i savremene DNK iz P~inje dokazuju visok
stepen me|usobne srodnosti, uprkos tri milenijuma koja ih dele. Budu}a istra`ivanja
usmeri}e se na karakterizaciju drevnog gra{ka i pojedine korake u njegovoj
domestikaciji.
Klju~ne re~i: gra{ak, domestikacija, drevna DNK, urov
THE ANCIENT DNA OF GRAIN LEGUMES AS A TIME
MACHINE TO THE EARLY DAYS OF THEIR CULTIVATION
Grain legumes such as pea (Pisum sativum L.) and bitter vetch (Vicia ervilia (L.)
Willd.) are considered one of the first domesticated plants, most likely in modern
Syria at least 10,000 years ago. From there, they quickly spread over Anatolia and entered the post-glacial Europe via the Balkans. Recent advances in the research on human and animal ancient DNA (aDNA) raised interest in the analysis of the extracted
aDNA from charred grain legume seeds. The first known worldwide success has been
achieved in the extraction and the validation of the aDNA from the charred pea and
bitter vetch seeds from the Early Iron Age settlement of Hissar near Leskovac, about
3,200 years old. At the same time, the old references on the existence of wild pea
(Pisum sativum subsp. elatius) in the flora of Serbia have been confirmed by the discovery of few populations in the valley of river P~inja. Preliminary results of a comparative analysis of the ancient pea DNA from Hissar and the modern pea from
P~inja prove that the two, despite three millennia in between, are rather related to
each other. Further analysis aims at the characterisation of the ancient pea and the
steps within its domestication.
Key words: ancient DNA, bitter vetch, domestication, pea
58
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA VARIJABILNOST I HERITABILNOST AGRONOMSKIH
OSOBINA OPLEMENJIVA^KE KOLEKCIJE GENOTIPOVA [email protected]
Dejan Sokolovi}1, Sne`ana Babi}1, Zorica Tomi}2, Jasmina Milenkovi}1,
Bojan An|elkovi}1, Bogovid @ivkovi}1 i Mirjana Cvetkovi}1
1Institut za krmno bilje, Kru{evac
2Institut za sto~arstvo, Zemun
Je`evica (Dactylis glomerata L.) je jedna od najva`nijih krmnih trava umerenog
klimata Evrope i Srbije. U prirodnim stani{tima u Srbiji prisutna je sa razli~itom
brojno{}u od nizija pa sve do visokih planina preko 100 mnv. Komponenta je
razli~itih sejanih travno-leguminoznih sme{a. Odlikuje se izra`enom adaptabilno{}u i
visokim prinosom kvalitetne krme.
Geneti~ka varijabilnost oplemeni~ake germplazme za sve agronomski vazne osobine
predstavlja osnovni preduslov uspe{ne selekcije. Najva`niji izvor varijabilnosti ~ine
autohtone populacije, ali i introdukovani genotipovi razli~itog porekla.
U ovim istra`ivanjima 60 genotipova je`evice (35 iz semena i 25 vegetativnih) su analizirana da bi se utvrdila geneti~ka varijabilnost i heritabilnost naijbitnijih osobina,
njihova me|uzavisnost i diverzitet unutar germplazme. 16 morfolo{kih i agronomskih
osobina, kao i hemijski sastav i prinos suve materije su prou~avani tokom dve godine.
Posebna pa`nja posve}ena je vremenu stasavanja i klasanja genotipova. Svi podaci
obra|eni su analizom varijanse i klaster analizom. Krajni rezlutat ovih istra`ivanja
predstavlja selekcija genotipova za dalje oplemenjivanje krmnih sorti razli~itog stasavanja.
Klju~ne re~i: geneti~ka varijabilnost, heritabilnost, je`evica, oplemenjiva~ka germplazma, korelacije
GENETIC VARIABILITY AND HERITABILITY OF AGRONOMIC TRAITS
OF ORCHARDGRASS GENOTYPES BREEDING COLLECTION
Cocksfoot (Dactylis glomerata L.) is one of the most important cool season forage
grasses in Europe and Serbia. In natural habitats in Serbia Dactylis glomerata L. is
present, with different covering, from lowland to mountains over 1000m a.s.l. It shows
great adapt abil ity, high yield of qual ity for age as com po nent of dif fer ent sown
grass-legume mixtures. Genetic variability of breeding germplasm for all important
agronomical traits represents basic prerequisite for successful selection. Most important source of variability are natural populations, but also imported genotypes of diverse origin. In this research 60 cocksfoot genotypes (35 from seed and 25 vegetative
accessions) were evaluated in order to determine genetic variability and heritability
of important traits, their correlation and diversity within germplasm. 16 morphological and agronomical traits, dry matter yield and chemical composition were investigated during two years period. Special attention was directed to maturity time and
tillering phenophase. All data were processed by ANOVA and cluster analysis. All
that has been resulted in selection of promising genotypes for further forage cultivars
breeding.
59
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KORI[]ENJE MUTIRANIH GENOTIPOVA U SELEKCIJI PARADAJZA
NA SPECIFI^NA SVOJSTVA U INSTITUTU ZA POVRTARSTVO
Jasmina Zdravkovi}1, @ivoslav Markovi}1, Milan Zdravkovi}1, Rado{ Pavlovi}2,
Mirjana Mijatovi}1, Tomislav @ivanovi}3 i Nenad Pavlovi}1
1Institut
za povrtarstvo, Smederevska Palanka
fakultet, ^a~ak
3Poljoprivredni fakultet, Zemun
2Agronomski
Da bi se na{ao superdominantan gen za prinos, ispitana je kolekcija od 350 uzoraka
(kroz vi{egodi{nja ispitivanja), sa mutacijama gena koji imaju razli~itu ekspresiju u
razli~itim fazama rasta i razvi}a paradajza: veli~inu ploda, oblik listova, arhitekturu
biljke, broj plodova, itd. Danas je u semenskoj produkciji Instituta za povrtarstvo je
26 hibrida i dva u procesu priznavanja, razli~itih namena i karakteristika. Naj~e{}e
kori{}eni mutirani geni kori{}eni selekciji paradajza u Institutu za povrtarstvo su: sp
(self pruning) koji je ugra|en u determinantne hibride (Balkan F1, Marko F1, Rebus
F1) i sorte (Narvik SPF, SP-109, Adonis), u (iniform ripening) - Lido F1, Atina F1,
[ampoin F1, Danubius F1, rin (ripening inhibitor) - Nada F1, [ampion F1, Sef F1, Sidra
F1, Rebus F1, j (jointless) - Narvik, Adonis, SP-109, c (potato leaf) - Mi-13, Enigma F1,
Rebus F1, Ph -2 (Phytophtora infestans resistance) - Luna F1, Zlatni jubilej F1, Sef F1.
Kvalitet plodova paradajza defini{u dve grupe gena koji odre|uju organolepti~ka
svojstva i hranljivi sastav. Organolepti~ki kvalitet podrazumeva ukus i miris, boju i
teksturu plodova koje su uslovljene sortnim razlikama, nutritivnim re`imom biljaka,
fazom zrenja i berbom. Malo se zna o genetskoj regulaciji koja je odgovorna za
varijaciju ovih osobina.
Klju~ne re~i: selekcija paradajza, mutirani genotipovi, specifi~na svojstva
USAGE OF MUTANT GENOTYPES IN TOMATO SELECTION FOR
SPECIFIC TRAITS AT THE INSTITUTE FOR VEGETABLE CROPS
In order to find a superdominant gene for yield, a collection of 350 samples with gene
mutations and different expression in different phases of growth and development:
fruit size, leaf shape, plant architecture, number of fruits, etc. has been studied
(trough multi-year research). Current seed production at the Institute for Vegetable
Crops in Smederevska Palanka includes 26 hybrids and two in acceptation process, of
different purposes and characteristics. The most common mutation genes in tomato
selection at the Institute are: sp (self pruning) inbuilt in determinant hybrids (Balkan
F1, Marko F1, Rebus F1) and varieties (Narvik SPF, SP-109, Adonis), u (uniform ripening) - Lido F1, Atina F1, [ampoin F1, Danubius F1, rin (ripening inhibitor) - Nada F1,
[ampion F1, Sef F1, Sidra F1, Rebus F1, j (jointless) - Narvik, Adonis, SP-109, c (potato
leaf) - Mi-13, Enigma F1, Rebus F1, Ph -2 (Phytophtora infestans resistance) - Luna F1,
Zlatni jubilej F1, Sef F1. Quality of tomato fruits is defined by two groups of genes
which determinate the organoleptic traits and nutrition composition. Organoleptic
quality includes taste and smell, colour and texture of fruits established in variety differences, nutritive regime of plants, maturity phase and yield. Little is known about
gene regulation, responsible for variation of these traits.
Key words: tomato breeding, mutant genotypes, specific traits
60
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VARIJABILNOST BELOG LUKA ROKAMBOLA Allium sativum sagitatum L.
Ivo Djinovi}, Biljana Milanovi} i Neboj{a \inovi}
Superior d.o.o., Velika Plana
Prva frakcija, sitan, bio je zastupljen sa svega 3% po broju jedinki, druga, srednje
krupan, 18%, tre}a, krupan 37% i vrlo krupnog je bilo najvi{e, 40%. Jedan broj
viviparnog arpad`ika, oko 1%, daje klasi~nu biljku rokambola, glavicu sa ~enovima,
cvetonosnim stablom i cvasti. Prose~an broj arpad`ika za svih 50 klonova iznosi 76
komada ili 255 grama sadnog materijala. Variranje je bilo od 4 do 154 arpad`ika po
klonu, a u gramima, od 12 do 373! Najprinosniji po broju potomaka bio je klon 32, sa
154 (312 g) a po prinosu klon 49 sa 373 g koliko je izmerilo njegovo potomstvo od 96
arpad`ika. Najkrupniju glavicu iz ~enova izmerili smo u klonu devet, 96 g. Neki od
njenih ~enova imaju masu od 18 grama.
Biljke rokambola dobijene iz sitnog arpad`ika, <8 mm, dale su glavicu prose~ne mase
od 13 g, sa variranjem od 6-23, one iz frakcije 8-12 mm 26,1 g (variranje 11-45), frakcija
12-16 mm daje prose~nu glavicu od 35,5 g (variranje 12-51) i one >16 mm 38,8 g, uz
variranje od 18 do 77 grama. Ako se za index 100 uzme masa glavica iz najkrupnijeg
arpad`ika, onda sitan ima 33%, srednje krupan 67% i krupan 91%, {to bi, pri istom
broju biljaka po jedinici povr{ine, davalo takav odnos u dobijenim prinosima. To zna~i
da krupno}a arpad`ika rokambola ima odlu~uju}u ulogu u formiranju prinosa. Sitnog,
prose~ne mase 0,28 g, u kilogramu ima 3751 komad. Ako se za standardnu gustinu
useva belog luka uzme 100 biljaka/m2, onda je potrebna kol~ina sadnog materijala ove
frakcije 267 kg po hektaru. Srednje krupnog, mase 0,86 g trebalo bi 860 kg, krupnog
(1,85 g/kom) 1852 kg i vrlo krupnog, mase 4,34 g/kom, 4348 kg.
Klju~ne re~i: beli luk rokambol, viviparija, varijabilnost
VARIABILITY OF VIVIPARUM GARLIC - Allium sativum sagitatum L.
The first fraction, small, was represented by only 3%, the number of plants, the second, medium-large, 18%, the third, 37% of large and very large was the highest, 40%.
A number of viviparous bulbs, about 1%, give a classic viviparous garlic plant, head
with cloves, stem and blossom. The average number of garlic bulbs for all 50 clones is
76 pieces or 255 grams of planting material. Variation, regarding in number of bulbs,
was from 4 to 154 per clone, from 12 to 373 in grams!
Best yielding, by the number of garlic bulbs of clones, was 32, with 154 (312 g) and the
yield of clone 49 to 373 g as a measure of his descendants garlic bulbs 96. The best
weight head of cloves are measured in clone 9- 96 g. Some of her cloves have a mass of
18 grams.
Plants derived from small garlic bulbs, <8 mm, provided the head of an average
weight of 13 g, with varying from 6-23, those from 8-12 mm fraction 26.1 g (varying
11-45), fraction 12-16 mm gives the average head of 35.5 g (varying 12-51) and those>
16 mm 38.8 g, with varying from 18 to 77 grams. If the index took 100 head of mass
best large garlic bulbs, it has 33% of small, middle large 67%, large 91%, which, at the
same number of plants per unit area, gave such a relationship in the yields obtained.
This means that the coarseness garlic bulbs a decisive role in the formation of yield.
Petty, mean mass 0.28 g, in a 3751 pound piece. If the density of the standard crop of
garlic took 100 plants /m2, then you need quantity planting material of this fraction of
267 kg per hectare. Medium large, weighing 0.86 g should be 860 kg, large (1.85 g/pc)
1852 kg and a very large, weighing 4.34 g/pc, 4348 kg.
Key words: Rocambol garlic, viviparum, bulbs, variability
61
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
MOGU]NOST PRIMENE MOLEKULARNIH RAPD
MARKERA U SELEKCIJI CRNOG LUKA
Nenad Pavlovi}1, Jasmina Zdravkovi}1, Dejan Cviki}1, Milan Zdravkovi}1,
Sla|an Ad`i}1, Suzana Pavlovi}1 i Gordana [urlan-Momirovi}2
1Institut za povrtarstvo, Smederevska Palanka
2Poljoprivredni fakultet, Zemun
U cilju procene divergencije na molekularnom nivou roditeljskih linija koje su
kori{}ene u dialenom ukr{tanju sa ciljem dobijanja superiornog potomstva (hibrida
F1 generacije) u Institutu za povrarstvo, ura|ana je molekularna analiza primenom
pet RAPD markera kod pet roditeljskih parova. Ovim putem je sagledan njihov
geneti~ki polimorfizam kao i mogu}nost njihove dalje primene u oplemenjiva~kim
programima. Informacije dobijene ovim istra`ivanjem predstavljaju pionirski
poduhvat u primene molekularnih markera na crnom luku u Srbiji.
Analize su izvr{ene upotrebom RAPD prajmera, za koje je u ranijim istra`ivanjima
utvr|en visok stepen polimorfizma. U svih pet slu~ajeva je do{lo do amplifikacije
odgovaraju}ih DNA segmenata. Ukupno je o~itano 50 bendova, du`ine fragmenata u
rasponu od 500 do 3000 bp. Broj polimorfnih bendova po prajmeru se kretao od 8 do
13. U na{em istra`ivanju na nivou svih analiziranih prajmera, utvr|en je visok stepen
polimorfizma izme|u analaziranih genotipova. Na osnovu UPGMA dendograma
analizirani genotipovi su razvrstani u dva osnovna klastera i dva subklastera.
Klju~ne re~i: crni luk, RAPD, geneti~ki polimorfizam
THE USE OF RAPD MARKERS IN ONION SELECTION
In order to estimate, at the molecular level, the divergence of parental lines that were
used in diallel crossbreeding for production of superior offspring (F1 generation hybrids) at the Institute for Vegetable Crops, the molecular analysis using five RAPD
markers for five pairs of parents has been performed. It gives an insight into their genetic polymorphism and the possibility of their further use in breeding programs. Information from this research has pioneered the application of molecular markers of
onion in Serbia.
Analyses were performed using the RAPD primers, which in previous studies established a high degree of polymorphism. In all five cases there was a corresponding amplification of DNA segments. From totally 50 bands analyzed, the length of fragments
ranged from 500 to 3000 bp. Number of polymorphic band per example was 8 to 13. In
our research at the level of the analyzed primers, a high degree of polymorphism between analyzed genotypes has been found. Based on UPGMA dendogram, analyzed
genotypes were divided into two main clusters and two subclusters.
Key words: onion, RAPD, genetic polimorphism
62
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OPLEMENJIVANJE OSOBINA KVALITETA
LUCERKE (Medicago sativa ssp. sativa L.)
Dragan Mili}, Slobodan Kati}, \ura Karagi}, Sanja Vasiljevi},
Branko Milo{evi} i Aleksandar Miki}
Institut za ratarstvo i povrtarstvo, Novi Sad
Pra}eni su kvantitativni geneti~ki parametri za sadr`aj sirovih proteina sadr`aj
neutralnih deterd`ent vlakana (NDF), sadr`aj kiselih deterd`ent vlakana (ADF) i
sadr`aj kiselih vlakana lignina (ADL). Analizirano je F 1 potomstovo nastalo
dialelnim ukr{tanjem (uklju~uju}i reciproke), izme|u 5 neinbredovanih roditeljskih
populacija razli~itog geografskog porekla. Dialelna analiza je ukazala na zna~ajno
ve}e efekte op{tih kombinacionih sposobnosti (OKS), u odnosu na efekte posebnih
kombinaconih sposobnosti (PKS) za ve}inu ispitivanih osobina u analizama cele
biljke. Analiza F1 potomstava ukazuje na zan~aj genotipa za skoro sve pra}ene
komponente kvaliteta (osim sadr`aja ADF-a). Visoko zna~ajni maj~inski efekti
registrovani su za sadr`aj NDF-a i ADF-a na nivou cele biljke, ali u listovima i
stabljikama ne. Na~in nasle|ivanja je bio prete`no aditivan, zato {to su efekti OKS
ve}i od efekata PKS za ve}inu pra}enih osobina. Najve}e vrednosti heritabilnosti
zabele`ene su za sadr`aj NDF-a (h =0,26), i sirovih proteina (hn= 0,24) u celoj biljci i
li{}u (0,15 and 0,11), dok u stabljikama osobine najve}e naslednosti su sadr`aj ADF-a
(hn= 0,38) i ADL-a (hn= 0,22). Dobijeni rezultati ukazuju da sadr`aj sirovih proteina,
NDF and ADF bi bili efikasniji kriterijum u oplemenjivanju na nivou cele biljke, dok
bi sadr`aj ADF-a i ADL-a bilo va`nije istra`ivati u stabljikama u oplemenjivanju na
pobolj{anu hranljivu vrednost lucerke.
Klju~ne re~i: kombinacione sposobnosti, osobine kvaliteta, varijansa, heritabilnost
BREEDING AND IMPROVEMENT OF QUALITY
TRAITS IN ALFALFA (Medicago sativa ssp. sativa L.)
Quantitative genetic parameters were estimated for crude protein (CP) content, neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin
(ADL) content. A 5x5 diallel design with reciprocals and without selfing among parents from geographically different populations were studied. In the diallel study, effects due to general combining ability (GCA) were higher than those due to specific
combining ability (SCA) for majority of the tested characters in analyses of whole
plant. The F1 progeny effect was significant for almost all characters (except ADF
content). The maternal effect was highly significant for NDF and ADF in whole plant,
and no significance were found in leaves and stems for these traits. The inheritance
was predominantly additive because portion of GCA effects was higher than SCA for
most following traits. The highest values of narrow sense heritability’s were found for
NDF (hn= 0.26) and CP (hn= 0.24) in whole plant, and in leaves (0.15 and 0.11), while
in stems most heritable traits are ADF (hn= 0.38) and ADL (hn= 0.22) content. As a
consequence, CP, NDF and ADF content would be more efficient selection criteria in
whole plant analyses, while ADF and ADL content would be more interesting to investigate in stems, in a breeding program for improved nutritive value of alfalfa.
Key words: combining ability, heritability, quality traits, variance
63
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA VARIJABILNOST AGRONOMSKIH OSOBINA
RAZLI^ITIH GENOTIPOVA LUCERKE (Medicago sativa L.)
Ratibor [trbanovi}1, Jasmina Radovi}1, Gordana [urlan-Momirovi}2,
Zoran Lugi}1, Goran Jevti}1, Jordan Markovi}1 i Tanja Vasi}1
1Institut za krmno bilje, Kru{evac
2Poljoprivredni fakultet, Zemun
Cilj istra`ivanja je utvr|ivanje varijabilnost najzna~ajnijih agronomskih svojstava 15
genotipova lucerke (Medicago sativa L.) doma}eg i stranog porekla. Ogled je
postavljen na oglednom polju Instituta za krmno bilje u Kru{evcu. U uslovima
pojedina~ne setve, u drugoj i tre}oj godini iskori{}avanja, prou~avane su morfolo{ke
osobine (visina biljaka, regeneracija biljaka nakon kosidbe, du`ina i {irina srednjeg
listi}a, pre~nik stabla, broj izdanaka i broj bo~nih grana), komponente prinosa
biomase (prinos zelene mase i suve materije po biljci, po otkosima i ukupno), kao i
kvalitet biomase (mineralne materije, sirovi proteini, sirova celuloza, sirove masti i
BEM) ispitivanih genotipova. Izra~unate komponente varijabilnosti ukazuju na
postojanje zna~ajne varijabilnosti kako izme|i, tako i unutar ispitivanih sorti, u
zavisnosti od otkosa i godine iskori{}avanja, za ve}inu ispitivanih osobina. Utvr|en je
visok udeo geneti~ke varijanse u ukupnoj varijansi za ve}inu osobina, {to potvr|uju i
koeficijenti heritabilnost u {irem smislu, koji su se kretali od 0,37 do 0,72.
Hijerarhijskom klaster analizom potvr|eno je postojanje geneti~ke divergentnosti
izme|u ispitivanih sorti.
Klju~ne re~i: lucerka, varijabilnost, agronomske osobine, heritabilnost
GENETIC VARIABILITY FOR AGRONOMICAL TRAITS OF
DIFFERENT ALFALFA (Medicago sativa L.) GENOTYPES
The aim of the in ves ti ga tion was to es ti mate vari abil ity of the most im por tant
agronomical traits of 15 alfalfa genotypes (Medicago sativa L.) of domestic and foreign
ori gin. The field experi ment was conducted at the Institute for forage crops in
Krusevac. During second and third year of utilization on space plant, morphological
traits (height of plants, regeneration of plants after cutting, lenght and width of central leaflet, number of stems per plant and number of internodes per stem), the yield
components of biomass (the green and dry mass yield per plant per cuts and total) and
forage quality (mineral matter, crude protein, crude fiber, crude fat and NFE content) were determined. The components of variability showed high variability among
and between varieties, for the most investigation traits. Results were strongly dependent on cut and year. Proportion of the genetic variance in total variance for most traits
was high, as evidenced by broad-sense heritability coefficients which ranged from
0.37 to 0.72 was obtained. Hierarchical cluster analysis confirmed genetic divergence
among varieties.
Key words: alfalfa, variability, agronomical traits, broad-sense heritability
64
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ INBREEDING DEPRESIJE NA AGRONOMSKE OSOBINE
RAZLI^ITIH GENOTIPOVA LUCERKE (Medicago sativa L.)
Jasmina Radovi}, Dejan Sokolovi}, Zoran Lugi},
Ratibor [trbanovi}, Sne`ana An|elkovi} i Tanja Vasi}
Institut za krmno bilje, Kru{evac
Prirodna autotetraploidnost, izra`ena autosterilnost i entomofilni na~in opra{ivanja
~ine lucerku varijabilnom vrstom, pa samooplodnja kod nje izaziva jaku inbreeding
depresiju. Uticaj samooplodnje na agronomske osobine lucerke ispitivan je u
mati~njaku porodica i S1, S2 i S3 samoplodnih linija, koje vode poreklo od istog
geneti~kog materijala. Rezultati ukazuju na jasno uo~ljiv negativan uticaj
samooplodnja na pra}ene parametre u obe godine istra`ivanja. Inbridovanje je ve}
kod S1 linija dovelo do zna~ajnog pada prinosa zelene i suve materije (do 53,2%),
broja izdanaka po biljci (do 26.4%) i visine biljaka (do 12,64%), dok je negativan
uticaj samooplodnje bio ne{to manje izra`en za broj internodija po stabljici i veli~inu
lista (do 7,28%). Utvr|ene su zna~ajne razlike izme|u ispitivanog materijala u
intenzitetu in breed ing depresije. Smanjenje vrednosti za pra}ene osobine je
nastavljeno i kod S2 i S3 linija ali u ne{to slabijem intenzitetu. Primetno je smanjenje
varijabilnosti za pra}ene osobine u zavisnosti od nivoa samooplodnje, pa se
koeficijent varijabilnosti za prinos suve materije kretao se od 38,1% kod porodica, do
24,9% kod S3 linija, za izdanke 36,8% do 14,7% kod S3.
Klju~ne re~i: lucerka, inbreeding depresija, agronomske osobine
EFFECT OF INBREEDING DEPRESION ON PRODUCTIVE TRAITS OF
DIFFERENT ALFALFA (Medicago sativa L.) VARIETIES
Alfalfa is tetraplioid, self-incompatible, entomophilies and highly variable species,
and its self-fertilization causes severe inbreeding depression. Effect of self-fertilization on agronomic traits of alfalfa was investigated in nursery in families and self-fertilizing lines of the first, second and third generation, originating from the same genetic material. Negative effect of self-fertilization on investigated traits was evident in
both years of experiment. Inbreeding has caused already in S1 lines considerable decrease of green and dry matter yield (up to 53,2%), number of steam per plant (up to
26.4%) and plant height (up to 12,64%), whereas negative effect of self-fertilization
was less expressed in regard to number of internodes per stem (up to 7,28%). Alfalfa
varieties showed differences in degree of inbreeding depression. Decrease of values
of examined traits continued in lower intensity in S2 and S3 lines. Also the decrease in
variability for observed traits depending on the level of self-fertilization was registered, so variability coefficient for yield of dry matter varied from 38, 1% in families
to 24, 9% in S3 lines, for young shoots 36, 8% to 14, and 7% in S3.
Key words: alfalfa, inbreeding depresion, agronomic traits
65
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA VARIJABILNOST I KORELATIVNI ODNOSI
[email protected] AGRONOMSKIH SVOJSTAVA GENOTIPOVA BELE
DETELINE (Trifolium repens L.) RAZLI^ITOG POREKLA
Zoran Lugi}, Jasmina Radovi}, Dejan Sokolovi},
Sne`ana Babi}, Ratibor [trbanovi} i Goran Jevti}
Institut za krmno bilje, Kru{evac
U radu su prikazani rezultati predselekcionih istra`ivanja 21 genotipa bele deteline
razli~itog porekla (divlje i selekcionisane populacije, doma}e i strane sorte).
Ispitivanja su vr{ena na pojedina~nim biljkama rasa|enim na me|urednom rastojanju
1x1m na oglednom polju Instituta za krmno bilje Kru{evac. Tokom dvogodi{njih
istra`ivanja pra}ene su slede}e traits: visina biljke, du`ina lista, {irina lista, prinos
zelene mase po biljci, prinos suve mase po biljci i {irenja bokora i prinos semena po
biljci. The results for both years for all traits were analyzed by ANOVA. Cluster analysis was performed using software Statistica for Windows for all investigated traits.
Rezultati ukazuju na visok potencijal, posebno doma}ih genotipova za prinos krme i
semena i mogu}nost njihovog kori{}enja za u programima oplemenjivanja bele
deteline za razli~ite namene. Tako|e, konstatovana je viskoka varijabilnost, kako
pojedina~nih biljaka u okviru, tako i izme|u ispitivanih genotipova za ispitivana
svojstva. Najja~i korelativni odnosi su zabele`eni za prinos zelene mase po biljci,
visinu biljke i {irinu bokora a najslabiji za veli~inu lista i prinos semena po biljci.
Klju~ne re~i: bela detelina, genotip, prinos suve materije, varijabilnost
GENETIC VARIABILITY AND CORRELATIVE RELATIONSHIPS
OF THE MOST IMPORTANT AGRONOMIC TRAITS OF WHITE CLOVER
(Trifolium repens L.) GENOTYPES OF DIFFERENT ORIGINS
The results of pre-selection research of 21 white clover genotypes of different origins
(wild and selected population, domestic and foreign varieties) are presented in this
paper. Research was performed on individual plants with the inter-row distance of
1x1m at the experimental field of the Institute for forage crops, Kru{evac. During the
two-year study following traits were observed: plant height, leaf length and leaf width,
forage yield per plant, dry mass yield per plant, canopy growth and seed yield per
plant. The results for both years for all traits were analyzed by ANOVA. Cluster analysis was performed using software Statistica for Windows for all investigated traits.
The results indicate high potential, particularly of domestic genotypes, for forage and
seed yield and the possibility of their use in breeding programs for white clover for
different purposes. Also, highly-variability, both within individual plants and between
genotypes for the investigated properties was determined. The strongest correlative
relationships were determined for forage yield per plant, plant height and width of
the canopy, and the lowest for the size of leaf and seed yield per plant.
Key words: white clover, genotype, dry matter yield, variability
66
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NAJZNA^AJNIJE MORFOLO[KO-BIOLO[KE OSOBINE U
OPLEMENJIVANJU CRVENE DATELINE (Trifolium pratense L)
Sanja Vasiljevi}1, Irena ]ali}2, Ramadan Salem Ahsyee3, Gordana
[urlan-Momirovi}2, \ura Karagi}1,Tomislav @ivanovi}2 i Branko Milo{evi}1
1Institut za ratarstvo i povrtarstvo, Novi Sad
2Poljoprivredni fakultet, Zemun
3Fakultet prirodnih nauka (Zentan), Libija
Crvena detelina (Trifolium pratense L) je druga po zna~aju vi{egodi{nja krmne leguminoza Noviji pravci u oplemenjivanju crvene deteline su usmereni u pravcu
kreiranja usko specijalizovanih sorti, visoke adaptibilnosti, te je od naro~itog je
zna~aja da se na po~etnom selekcionom materijalu izvr{i evaluacija pojedinih morfolo{ko-biolo{kih osobina (vreme cvetanja, forma rasta, perzistentnost) koje
neposredno ili posredno uti~u na visinu prinosa krme i du`inu eksplatacije deteli{ta.
Pojam po~etka cvetanja kod crvene deteline oduvek je bio od naro~itog zna~aja, s
obzirom da se u odnosu na ovu pojavu vr{i osnovna klasifikacija na: ranostasne,
srednjestasne i kasnostasne forme. S druge strane utvr|ena je povezanost pojave
cvetanja kod crvene deteline u godini setve sa slabijom perzistentno{}u (tzv.
dvootkosni tip). Ne{to ve}a perzistentnost je utvr|ena kod stoloniferum tipa crvene
deteline. Saglasno UPOV-om deskriptoru iz 2001. godine najnovija klasifikacija
forme rasta obuhvata pet razli~itih tipova, (erect, semi-erect, intermediate, semi-prostrate, prostrate), pri ~emu je utvr|eno da je prostratum tip perzistentniji u odnosu na
erectum tip, te da bi on mogao imati prekti~nu vrednost, prilikom ukr{tanja sa
komercijalnim sortama, kako bi se pove}ala njihova perzistentnost
Klju~ne re~i: crvena detelina (Trifolium pratense L), oplemenjivanje, morfolo{ko-biolo{ke karakteristike
THE MOST IMPORTANT MORPHOLOGICAL-BIOLOGICAL
CHARACTERISTICS IN RED CLOVER (Trifolium pratense L) BREEDING
Red clover (Trifolium pratense L.) is the second most important forage legume. The
current breeding objectives are aimed at the development of highly adapted cultivars.
It is particularly important to evaluate initial breeding material for the morphological
and biological characteristics (time of flowering, growth habit, persistence) that indirectly or directly affect the forage yield performance and crop persistence. Beginning
of flowering has always been considered as an important parameter in red clover because is served to classify cultivars into early, mid-season and late ones. Furthermore,
correlations have been found between the flowering of red clover in the year of planting and poor persistence (the so-called two-cut “medium type). A somewhat higher
red clover persistence has been found in the prostrate type. According to UPOV
descriptor from 2011, red clover growth types include five different types, (erect,
semi-erect, intermediate, semi-prostrate, prostrate). The descriptor further maintained
that the prostrate type was more persistent than the erect type, and that could be used
for crossing to commercial cultivars, to increase their persistence.
67
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
STVARANJE SORTI JEDNOGODI[NJIH MAHUNARKI SA
POVE]ANOM OTPORNOSTI NA NISKE TEMPERATURE
Vojislav Mihailovi}1, Aleksandar Miki}1, Sanja Vasiljevi}1, \ura Karagi}1,
Branko Milo{evi}1, Branko ]upina2 i Slobodan Kati}1
1Institut za ratarstvo i povrtarstvo, Novi Sad
2Poljoprivredni fakultet, Departman za ratarstvo i povrtarstvo,
Novi Sad
Me|u ekonomski va`nim jednogodi{njim mahunarkama nalaze se gra{ak (Pisum
sativum L.), grahorice (Vicia spp.), bob (Vicia faba L.), so~ivo (Lens culinaris Medik.),
sastrica (Lathyrus sativus L.), naut (Cicer arietinum L.) i lu pine (Lupinus spp.).
Stvaranje sorti ovih useva sa pove}anom otporno{}u na ja~inu i du`inu trajanja niskih
temperatura neposredno doprinosi boljem kori{}enju poljporivrednog zemlji{ta i
ostalih prirodnih resursa, kao i pove}anju povr{ine gajenja mahunarki i porastu
proizvodnje kvalitetnih biljnih proteina za upotrebu u ishrani doma}ih `ivotinja. Prvi
korak predstavlja ispitivanje svih akcesija jedne zbirke jednogodi{njih mahunarki na
dejstvo niskih temperatura i odabir najboljih za ulogu roditelja u ukr{tanju i nastanku
nove geneti~ke varijabilnosti. U svakoj generaciji populacija nastalih ukr{tanjem,
odabiraju se linije po`eljnih agronomskih osobina i visoke otpornosti na niske temperature. Do sada, u Novom Sadu, stvoren je ve}i broj ozimih sorti krmnog gra{ka,
grahorica i boba, pri ~emu je poseban napredak na~injen kod sorte ozimog
proteinskog gra{ka afila tipa lista ‘Mraz’, priznate 2010. godine, sa izra`enom
ranostasno{}u i visokim prinosima zrna i proteina.
Klju~ne re~i: gra{ak, jednogodi{nje mahunarke, niske temper a ture, ozimost,
oplemenjivanje
DEVELOPMENT OF THE ANNUAL LEGUME CULTIVARS WITH
AN INCREASED RESISTANCE TO LOW TEMPERATURES
Among the economically important annual legumes are pea (Pisum sativum L.), vetches (Vicia spp.), faba bean (Vicia faba L.), lentil (Lens culinaris Medik.), grass pea
(Lathyrus sativus L.), chickpea (Cicer arietinum L.) and lupins (Lupinus spp.). Developing the cultivars of these crops with an increased tolerance to both intensity and
duration of low temperatures directly contributes to the better use of agricultural
land and other resources, as well as to increasing the cultivated area under legumes
and the production of quality plant protein for use in animal feeding. The first step is
screening of all accessions of a collection and the selection of the best ones as parental
components for crossings and creating new genetic variability. In every generation of
the hybrid populations, lines with desirable agronomic characteristics and high resistance to low temperatures are selected. So far, in Novi Sad, numerous cultivars of pea,
vetches and faba bean have been developed, with a particular progress in the autumn-sown semi-leafless protein pea cultivar Mraz, released in 2010, with a prominent earliness and with high yields of grain and protein.
Key words: annual legumes, breeding, low temperatures, pea, winter-hardiness
68
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ RASE 3 FUZARIOZNOG UVENU]A
NA POJEDINE SORTE I HIBRIDE PARADAJZA
Mladen \or|evi}, Bogoljub Ze~evi}, Radi{a \or|evi},
Jasmina Zdravkovi} i Mirjana Mijatovi}
Institut za povrtarstvo, Smederevska Palanka
Cilj ovog istra`ivanja je da se utvrdi kakav bi bio uticaj rase 3 fuzarioznog uvenu}a
paradajza na pojedine sorte i hibride ukoliko bi se ova rasa pojavila u Srbiji. U te
svrhe inokulisano je jedanaest sorti i hibrida paradajza ovim patogenom i to: Adonis,
Atina F1, Balkan F1, Danubius F1, Gru`anski zlatni, Jasmin crveni, Marko F1, Nada
F 1 , Narvik, Sp-109, Zlatni Jubilej F 1 , [ampion F 1 , primenom klasi~ne metode
inokulacije umakanjem povre|enog korena u suspenziju gljiva. Nakon 30 dana
ra|ena je procena pojave oboljenja upotrebom skale od 1 - 5. Nakon evaluacije, Nada
F1 i 129-Sprin su imali najni`u vrednost ADR-a (prose~ni nivo oboljenja) (2,9), i
obele`eni su kao tolerantni. Svi ostali smatrani su osetljivim sa vrednostima ADR
vi{im od 3,0, po slede}em rasporedu: Narvik (3,6), [ampion F1 (3,8), M-7 (3,9), Z.
Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5), Marko F1 and
M-10 (4,6). Na osnovu ovih rezultata mo`emo zaklju~iti da ako bi se rasa 3 Fusarium
oxysporum f. sp. lycopersici pojavila u Srbiji mogla bi zna~ajno da ugrozi proizvodnju
paradajza.
Klju~ne re~i: rasa 3, Fusarium oxysporum f. sp. lycopersici, otpornost, paradajz,
selekcija
EFFECT OF RACE 3 OF FUSARIUM WILT
ON SOME TOMATO VARIETIES
Aim of this study is to determine the impact of race 3 of fusarium wilt on some tomato
varieties if it occurs in Serbia. For this purpose eleven tomato varieties were inoculated with this pathogen: Adonis, Atina F1, Balkan F1, Danubius F1, Gru`anski zlatni,
Jasmin crveni, Marko F1, Nada F1, Narvik, Sp-109, Zlatni Jubilej F1, [ampion F1, by
applying classic method of inoculation by submersing the injured root in fungi suspension. Disease was assessed 30 days after inoculation using an ordinal scale range from
1 - 5. After assessment, Nada F1 and 129-Sprin had lowest average disease rating
(2,9), and marked as tolerant. All the other varieties were consider susceptible with
ADR values higher than 3,0 in the following order: Narvik (3,6), [ampion F1 (3,8),
M-7 (3,9), Z. Jubilej F1 (4,1), C. Jasmin (4,2), Danubius F1 (4,4), Balkan F1 (4,5),
Marko F1 and M-10 (4,6). Based on these results we can conclude that if the race 3 of
Fusarium oxysporum f. sp. lycopersici occur in Serbia it could seriously endanger tomato production.
Key words: race 3, Fusarium oxysporum f. sp. lycopersici, resistance, tomato, breeding
69
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VREME POJAVE PRVIH CVETONOSNIH STABALA, U PROCESU
VERNALIZACIJE, KOD SREDNJE RANIH I SREDNJE KASNIH
GENOTIPOVA KUPUSA GLAVI^ARA I NJIHOVIH F1 HIBRIDA
Sla|an Ad`i}1, Suzana Pavlovi}1, Jasmina Zdravkovi}1, Slaven Prodanovi}2,
Nenad Pavlovi}1, Zdenka Girek1 i Dejan Cviki}1
1Institut za povrtarstvo, Smederevska Palanka
2Poljoprivredni fakultet, Zemun
Mnoge dvogodi{nje vrste, u koje spada i kupus glavi~ar Brassica oleracea var. capitata
L., mogu u toku jedne sezone primenom niskih temperatura, u odre|enom trajanju,
da pre|u iz vegetativnog u generativni stadijum. U tom procesu gubi se jedna etapa
organogeneze - formiranje glavice. U Institutu za povrtarstvo u Smederevskoj Palanci
u toku sezone 2010-2011 na otvorenom polju, je postavljen dvofaktorijalni ogled (3
roka setve rasada i 4 ponavljanja) sa 6 genotipova (Nom, Scc i B, B x Scc; B x N; Scc x N)
divergentnim na du`inu vegetacionog perioda. Ispitan je uticaj giberelinske kiseline
(GA 3 ) u koncentraciji 300 ppm u odnosu na kontrolnu (netretiranu) grupu.
Dvofaktorskom analizom varijanse ispitana je zna~ajnost uticaja faktora: genotipova
i rokova setve kao i njihova interakcija, kako kod kontrolne grupe tako i kod biljaka
tretiranim GA3. Na pojavu cvetonosnih stabala u kontroli statisti~ku zna~ajnost
ispoljio je genotip i interakcija faktora, dok je kod tretmana sa GA 3 utvr|ena
statisti~ka za~ajnost oba faktora.
Klju~ne re~i: vernalizacija, cvetonosna stabla, genotip, rok setve, giberelinska kiselina
FIRST APPEARANCE OF FLOWER BRANCHES IN
VERNALIZATION PROCESS OF MIDDLE EARLY AND MIDDLE
LATE CABBAGE GENOTYPES AND THEIR F1 HYBRIDS
Many biennial species, such as head cabbage (Brassica oleracea var. capitata L.) can
transform from vegetative stadium, in conditions of low temperatures, to generative
stadium in one season. One phase of organogenesis is lost in this process - head forma tion. At the In sti tute for Veg e ta ble Crops in Smederevska Palanka dur ing
2010/2011 season a two-factorial trial has been set up in the open field, in three sowing periods and 4 replications, with 6 genotypes (Nom, Scc i B, B x Scc; B x N; Scc x N)
with divergent vegetation period. Aim of this study was to observed the effects of
GA3 (concentration 300 ppm) treatment on the appearance of flower branches, compared to control (untreated) group. By applying two-factorial analysis of variance the
significance of factor influence: genotype and sowing period and their interaction
both for control and treated plants, has been studied. On occurrence of flower branch
in control, genotype and factor interaction have been statistically significant, while
treatment GA3 showed statistical significance of both factors.
Key words: vernalization, flower branches, genotype, sowing period, gibberellic acid
70
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
IN VITRO KULTURA KAO DEO PROCESA
OPLEMENJIVANJA Brassica oleracea
Suzana Pavlovi}, Sla|an Ad`i}, Dejan Cviki} i Jasmina Zdravkovi}
Institut za povrtarstvo, Smederevska Palanka
Kod ~etrnaest genotipova kupusa (Brassica oleracea var. capitata L.), koji su deo
kolekcije Instituta za povrtarstvo, ispitana je sposobnost in vi tro regeneracije
pupoljaka. Ranih kupusa je bilo pet gonotipova, dok je kasnih kupusa bilo devet
genotipova. Bo~ni pupoljci biljaka gajenih na otvorenom polju kori{}eni su kao
eksplantati. Kod svih genotipova bo~ni pupoljci su pokazali visok procenat
regeneracije pupoljaka, od 80% do 100%. Inkubirani su na Murashige and Skoog’s
(MS) hranljivoj podlozi sa dodatkom 1.0 i 2.0 mg l -1 benziladenina (BA) ili
6-furfurilaminopurina (KIN) u kombinacija 0, 0.5 and 1.0 mg l-1 indole-3-butiri~ne
kiseline (IBA). Podloge koje su sadr`ale BA su bile optimalne, kako za regeneraciju
pupoljaka, tako i za njhovu kasniju multiplikaciju. Kod obe grupe genotipova najve}i
indeks multiplikacije je postignut na podlozi sa dodatkom 2.0 mgl-1 BA i 1.0 mgl-1
IBA, kod R9 ranog genotipa (IM 8.53) i kod K1 kasnog genotipa (IM 10.06). Kod R11
ranog genotipa i kod K29 i K75 kasnih genotipova nije bilo multiplikacije na podlozi sa
1.0 mgl-1 KIN (IM 1.00). Tako|e najmanji indeks multiplikacije kod svih genotipova
je prime}en na podlogama sa KIN (bez ili u kombinaciji sa IBA).
Klju~ne re~i: kupus, mikropropagacija, bo~ni pupoljci, indeks multiplikacije
IN VITRO CULTURE AS A PART OF Brassica oleracea BREEDING
Fourteen genotypes of cabbages (Brassica oleracea var. capitata L.), that are a part of
Institute for Vegetable Crops collection, were tested for their ability to regenerate
shoots in vitro. Five of them are early, while nine are late genotypes. Lateral buds
from plants grown in the open field were used as explants. In all genotypes lateral
buds showed the high percentage of shoot formation ranged from 80% to 100%. They
were incubated on Murashige and Skoog’s (MS) media supplemented with 1.0 and 2.0
mg l-1 of benzyladenine (BA) or 6-furfurylaminopurine (KIN) in combination with 0,
0.5 and 1.0 mg l-1 indole-3-butyric acid (IBA). The BA-supplemented media were optimal for both growth and multiplication of shoots. In both groups of genotypes, the
highest index of multiplication (IM) was achieved on medium supplemented with 2.0
mgl-1 BA and 1.0 mgl-1 IBA, in R9 early genotype (IM 8.53) and K1 late genotype (IM
10.06). R11 early and in K29 and K75 late genotypes had no multiplication on medium
with 1.0 mgl-1 KIN (IM 1.00). Also the lowest index of multiplication was observed in
all genotypes on media supplemented with KIN (without or in combination with
IBA).
Key worlds: cabbage, micropropagation, lateral buds, multiplication index
71
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA ANALIZA HIBRIDA DOBIJENIH UKR[TANJEM
RODITELJA IZ RAZLI^ITE GERMPLAZME LJUTE PAPRIKE
Bogoljub Ze~evi}1, Goran Todorovi}2, Jelena Damnjanovi}1,
Ana Vujo{evi}3 i Mladen \or|evi}1
2Institut
1Institut za povrtarstvo, Smederevska Palanka
za prou~avanje lekovitog bilja „Dr Josif Pan~i}“, Beograd
3Poljoprivredni fakultet, Zemun
Ljuta paprika zauzima veoma zna~ajno mesto u ukupnoj proizvodnji i potro{nji
paprike u Srbiji. Oplemenjivanje ljute paprike je zasnovano na primeni
konvencionalnih metoda, dok kori{}enje hibrida jo{ uvek nije zastupljeno kao u
zemljama sa visoko razvijenim semenarstvom. Prisustvo bogate germplazme sa
podru~ja Srbije, kao i sve ve}eg broja stranih hibrida, omogu}ava veoma dobru
osnovu za intenziviranje aktivnosti u istra`ivanjima na kori{}enju heterozisa. Na bazi
dialelnog ukr{tanja (bez recipro~nog) {est linija ljute paprike, poreklom sa razli~itih
lokaliteta, utvr|ene su vrednosti za op{te i posebne kombinacione sposobnosti, za
heritabilnost i za heterozis u F 1 generaciji. Visoko zna~ajne genotipske razlike
zabele`ene su za sve ispitivane osobine - du`inu ploda, {irina ploda i broj plodova po
biljci. Komponente varijanse za specifi~ne kombinacione sposobnosti su bile ve}e od
komponenti varijanse za op{te kombinacione sposobnosti za sve osobine.
Heritabilnost je bila relativno visoka i preko 55% za sve tri osobine. Najve}i efekat
heterozisa je utvr|en za osobinu broj plodova po biljci.
Klju~ne re~i: ljuta paprika, hibridi, heritabilnost, heterozis
GENETIC ANALYSIS OF HYBRIDS DEVELOPED BY CROSSING
PARENTS FROM DIFFERENT HOT PEPPER GERMPLASM
Hot pepper has very important place in the total pepper production and consumption
in Serbia. Breeding of hot pepper is based on the using of conventional methods while
the hybrid breeding is not present too as in the countries that are high developed in
the seed production. The presence of an abundance of hot pepper germplasm in Serbia, as well as a growing number of foreign hot pepper varieties, allowing a good basis
for intensifying activities in research on hybrid breeding. A half-diallel cross study of
six hot pepper genotypes from different localities was carried out. We investigated
general and specific combining ability, heritability and heterosis for fruit length, fruit
width and number of fruits per plant. Highly significant genotypic differences were
observed for all traits. Variance component due to specific combining ability were
larger than that due to general specific combining ability for each studied traits.
Heritability for all traits was rela tively high, over 55%. The great est ef fect of
heterosis is determined for number of fruits per plant.
Key words: hot pepper, hybrids, heritability, heterosis
72
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ INDUKCIJE POLNE EKSPRESIJE HEMIJSKIM
TRETMANIMA NA PRINOS DINJE
Zdenka Girek1, Jasmina Zdravkovi}1, Slaven Prodanovi}2, Dejan Cviki}1,
Sla|an Ad`i}1, Nenad Pavlovi}1 i Milan Zdravkovi}1
1Institut za povrtarstvo, Smederevska Palanka
2Poljoprivredni fakultet, Zemun
Obrazovanje pola kod dinje zavisi od nasledne (geneti~ke) kontrole i spoljnih
faktora. Ekspresija pola mo`e se izmeniti egzogenom primenom fitohormona i drugih
hemijskih jedinjenja. Cilj ovog istra`ivanja je bio ispitati uticaj hemijskih tretmana
(etrela, srebro nitrata i giberelinske kiseline) na masu ploda i broj plodova kod 23
genotipa dinje. Ogled je sproveden na eksperimentalnoj parceli Instituta za
povrtarstvo, Smederevska Palanka u toku 2010. i 2011. godine. Posmatran je efekat
tri razli~ita tretmana na osam roditelja i njihovih 15 hibrida, u odnosu na kontrolnu
grupu biljaka (bez tretmana). Utvr|ena je zna~ajna razlika mase ploda izme|u svih
prou~avanih genotipova (prose~na masa se kretala od 487 do 2609 grama). Kod
pojedinih genotipova je zabele`en porast mase od 57,37% (srebro nitrat), 35,20%
(etrel) i 47,45% (giberelinska kiselina) u odnosu na kontrolnu grupu. Najve}i broj
plodova kod ve}ine genotipova bio je u kontrolnoj grupi. Utvr|en je selekcioni
materijal dinje na koji dejstvo ovih hemijskih tretmana pozitivno uti~e a koji }e se
uklju~iti u dalja istra`ivanja.
Klju~ne re~i: etrel, giberelinska kiselina, srebro nitrat, Cucumis melo L.
EFFECTS OF CHEMICAL INDUCTION OF SEXUAL
EXPRESSION ON MELON YIELD
Melon sex expression depends of genetic control and environmental factors. Sex expression can be modified with application of exogenous phytohormones and other
chemical compounds. The aim of this study was to examine the effect of chemical
treatments (ethrel, silver nitrate, gibberellic acid, GA3) on the fruit weight and number of fruits per hectare in 23 melon genotypes. The experiment was conducted on an
experimental field of the Institute for Vegetable Crops, Smederevska Palanka in two
years (2010 - 2011). The effects of different treatments on the eight parents genotypes
and their 15 hybrids with regard to the control group (without treatment) was observed. Average fruit weight, ranged from 487 to 2609 grams, showed significant differences between the genotypes for this trait. The results showed that in some cases
average fruit weight was higher by 57.37 % (silver nitrate), 35.20% (ethrel) and
47.45% (GA3) in regard to the control group. The higher number of fruits was harvested from plants in control group. It was determined a melon breeding material,
with positive effect of chemical treatments on important fruit characteristics, which
will be included in further research.
73
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
MORFOLO[KE I HEMIJSKE ANALIZE LOKALNIH
POPULACIJA PER[UNA (Petroselinum hortense L)
Radi{a \or|evi}1, Bogoljub Ze~evi}1, Jelena Damnjanovi}1,
Mladen \or|evi}2 i Olivera \or|evi}2
1Institut za povrtarstvo, Smederevska Palanka
2Poljoprivredni fakultet, Zemun
Per{un (Petroselinum hortense L.) pripada familiji Apiaceae, po svojim prehrambenim i
lekovitim svojstvima spada u red najvrednijih povrtarskih kultura. U Srbiji per{un je
~esto gajeno povr}e po oku}nicama tako da se mo`e na}i relativno visok broj lokalnih
populacija.
U radu su prikazani rezultati ispitivanja 12 populacija per{una prikupljenih na
teritoriji Srbije. Njihove morfolo{ke i hemijske osobine pore|ene su sa standardnom
sortom Berlinski srednje dugi. U trogodi{njem periodu od 2006-2009. analizirane su
slede}e morfolo{ke osobine: prose~na masa korena, prose~na masa lista, pre~nik
vrata korena, pre~nik sredine korena i pojava sekundarnih korenova. Hemijskom
analizom odre|ena je suva materija, ukupni minerali, ukupni {e}eri i voda. Cilj je bio
da se ispita kvalitet prikupljenih populacija per{una i njihova mogu}nost uklju~ivanja
u proces selekcije radi stvaranja novih sorti per{una.
Klju~ne re~i: per{un, populacija, morfolo{ke i hemijske osobine, selekcija
MORPHOLOGICAL AND CHEMICAL ANALYSIS OF LOCAL
POPULATION OF PARSLEY (Petroselinum hortense L.)
Parsley (Petroselinum hortense L.) is a species of Petroselinum in the family Apiaceae.
Its nutritive and medicinal traits put it among the most valuable vegetable crops.
Parsley is very common in Serbian gardens, so the number of local populations is
quite large.
The paper deals with the study of 12 populations collected in Serbia. Their morphological and chemical traits have been compared to standard variety Berlinski srednje
dugi. During 2006-2009 the following morphological traits have been analyzed: average weight of root, the average weight of leaves, diameter of head, diameter of body
and appearance secondary roots. Chemical analysis revealed the quantity of dry matter, total minerals, total sugars and water. The aim was to study the quality of collected parsley populations and the possibility of it’s including in selection process in
order to create new varieties.
Key words: parsley, populations, morphological and chemical analysis, selection
74
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
SVOJSTVA KOJA UTI^U NA PRINOS CRNOG LUKA U USLOVIMA
ORGANSKE I KONVENCIONALNE PROIZVODNJE
Milka Brdar-Jokanovi}, Milan Ugrinovi}, Suzana Pavlovi}, Zdenka Girek,
Dejan Cviki}, Jasmina Zdravkovi} i Milan Zdravkovi}
Institut za povrtarstvo, Smederevska Palanka
Istra`ivanje je sprovedeno sa ciljem utvr|ivanja uticaja nekoliko svojstava crnog luka
na prinos u uslovima organskog i konvencionalnog gajenja. Dvogodi{nji poljski ogled
(2009, 2010) je postavljen na eksperimentalnoj parceli Instituta za povrtarstvo u
Smederevskoj Palanci i uklju~ivao je pet sorti crnog luka. Ogled je podrazumevao
ne|ubrene parcele, parcele |ubrene NPK (kontrole), mikrobiolo{kim |ubrivom i
stajnjakom (tretmani). Utvr|ene su zna~ajne razlike me|u sortama, godinama i
tretmanima u pogledu ispitivanih svojstava. Masa lukovice, broj lukovica po parceli i
pre~nik la`nog stabla su ispoljili pozitivne, a broj dana od setve do nicanja i du`ina
vegetacije negativne efekte na prinos u svim uslovima gajenja crnog luka. Prema
tome; ova svojstva mogu biti od koristi u selekciji crnog luka, bez obzira da li je re~ o
konvencionalnom ili organskom sistemu gajenja. Pre~nik lukovice, visina lukovice,
indeks lukovice, du`ina la`nog stabla i visina biljke su ispoljili razli~ite efekte na
prinos, zavisno od uslova gajenja.
Klju~ne re~i: crni luk, organska poljoprivreda
ONION YIELD CONTRIBUTING CHARACTERS IN ORGANIC
AND CONVENTIONAL GROWING SYSTEMS
The aim of this study was to investigate the effects of several traits on yield in onion
grown in organic and conventional environments. Two-year field trial (2009, 2010)
was set at the experimental field of the Institute for Vegetable Crops in Smederevska
Palanka and included five onion cultivars. The trial included unfertilized plots, plots
treated with NPK (controls), bacterial fertilizer and farmyard manure (treatments).
Significant differences have been found among the cultivars, growing seasons and
treatments, with respect to the investigated traits. Bulb weight, number of bulbs per
plot and neck diameter exhibited positive, while number of days from sowing to
emergence and vegetation period exhibited negative effects on yield in both conventional and organic environments. Therefore; these traits may be useful in onion
breeding, regardless of the growing system. The effects of bulb diameter, bulb height,
bulb index, neck length and plant height on yield differed, depending on the growing
system.
Key words: onion, organic agriculture
75
Sekcija / Section 4
Oplemenjivanje drvenastih
biljaka i vinove loze
Breeding of woody
plants and grapevine
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OPLEMENJVANJE BELOG BORA (Pinus sylvestris L.) U SRBIJI
Aleksandar Lu~i}1, Vasilije Isajev2 i Ljubinko Rakonjac1
1Institut za {umarstvo, Beograd, Srbija
2[umarski fakultet, Beograd, Srbija
U Srbiji je primenom masovne i individualne selekcije intenziviran rad na oplemenjivanju belog bora (Pinus sylvestris L.), detaljnim upoznavanjem: a) prirode
karaktera varijabilnosti (diskontinuirana, kontinuirana), i b) semenskih izvora, ~ije
seme najbolje odgovara odre|enim stani{tima. Gen-ekolo{ka varijabilnost belog bora
prou~avana je u vi{e populacija na pet lokaliteta u Zapadnoj i Jugozapadnoj Srbiji.
Analizama su obuhva}eni: oblik {i{arica, prose~an broj semenki po {i{arici,
koeficijent ekstrakcije, apsolutna masa semena, kao i klijavost semena u termostat
klijalici i proteinski polimorfizam semena. Procena interindividualne i interpopulacione genetske specifi~no sti belog bora obavljena je primenom RAPD i SSR
markera. U blizina U`ica, na povr{ini od 1,5 ha, sa 1750 sadnica iz 40 linija polusrodnika osnovana je semenska planta`a metapopulacione strukture. Dobijeni
rezultati su zna~ajni za bli`e poznavanje varijabilne i specifi~ne unutarvrsne geneti~ke
strukture belog bora, i kao polazna osnova procesa konzervacije, oplemenjivanja i
proizvodnje semena, sadnica i po{umljavanja ovom vrstom.
Klju~ne re~i: beli bor, oplemenjivanje, RAPD i SSR markeri
THE IMPROVEMENT OF SCOTS PINE (Pinus sylvestris L.) IN SERBIA
By mass and individual selection work on the improvement of Scots pine (Pinus
sylvestris L.) has been intensified in Serbia by a detailed study of: a) the nature of the
character of variability (discontinuous, continous), and b) seed sources which are
most favourable for specific sites. The genetic-ecological variation of Scots pine was
studied in the populations at five localities in western and south-western Serbia The
analysis includes: cone form, average number of seeds per cone, coefficient of extraction, absolute seed mass, and seed germination percentage in seed germinator thermostat and protein polymorphism of seeds. The estimations of genetics divergences
in Scots pine individuals and among populations were done by using RAPD and SSR
markers. Near town of U`ice, in the area of 1.5 ha, 1750 half sib seedlings from 40
linies have been planted to established a seed orchard of metapopulation structure.
The study results offer the basis for a better knowledge of the highly variable and
interspecies genetic structure, which is the initial means in the planning of the species
conservation, improvement processes and production of seeds, planting stock, and afforestation.
Key words: Scots pine, improvement, RAPD, SSR markers
78
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ME\UKLONSKI VARIJABILITET FENOLOGIJE
CVETANJA U SJEMENSKOJ [email protected] BIJELOG BORA
(Pinus sylvestris L.) PORED DOBOJA
Vanja Dani~i}1, Vasilije Isajev2, Milan Mataruga1 i Branislav Cvjetkovi}1
1[umarski
fakultet, Banja Luka, Bosna i Hercegovina
2[umarski fakultet, Beograd
U radu su prikazani faktori koji uti~u na fenologiju cvetanje i kvantitet i kvalitet
uroda u eksperimentalnoj klonskoj sjemenskoj planta`i bijelog bora (Pinus sylvestris
L.). Sjemenska planta`a je osnovana u blizini Doboja, Bosna i Hercegovina, od 20
klonova koji su zastupljeni sa 214 rameta. Istra`ivanja su obavljena u periodu
2004-2006. godine Rezultati trogodi{njih analiza, me|u- i unutarklonske
varijabilnosti: kod fenologije cvjetanja, kao i za obilnosti uroda. Rezultati obavljenih
istra`ivanja zna~ajni su za budu}e aktivnosti na osnivanju novih sjemenskih planta`a
vrsta drve}a kao i za unapre|enje uroda u postoje}im.
Klju~ne re~i: beljeli bor, semenske planta`e cvetanje, urod
INTER-CLONE VARIABILITY OF FLOWERING PHENOLOGY IN SCOTCH
PINE (Pinus sylvestris L.) SEED ORCHARD NEAR TOWN IN DOBOJ
This paper deals with factors influencing the flowering and the crop quantity and
quality in an experimental clone seed orchard of Scotch pine (Pinus sylvestris L.). The
seed plantation, established near town Doboj in Bosnia and Herzegovina, included 20
clones rep re sented by 214 ramets. The re search work was per formed dur ing
2004-2006. Three years of research work analyzed inter- and intra-clone variability in
ramets flowering phenology, which are considered to be the elements indicating qualitative and quantitative characteristics of flowering and crop yields. Therefore, the results obtained in this research are valuable for prospective activities of establishing
new seed plantations and enhancing the crop in existing ones.
Key words: Scotch pine, seed orchard, flowering, crop yields
79
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
IZDVAJANJE REGIONA PROVENIJENCIJA BUKVE U SRBIJI
PRIMENOM PROSTORNE ANALIZE GENETI^KOG DIVERZITETA
Vladan Iveti}1, Vasilije Isajev1, Ana Nikoli}2 i Milun Krsti}1
2Institut
1[umarski
fakultet, Beograd
za kukuruz “Zemun Polje”, Beograd
Rezultati prostorne analize geneti~kog diverziteta imaju prakti~nu primenu u
definisanju i razgrani~enju regiona provenijencija {umskih vrsta drve}a. Istra`ivanja u
ovom radu, obuhvatila su materijal iz 27 prirodnih populacija bukve u Srbiji.
Geneti~ka komponenta istra`ivanja bazira se na analizi RAPD markera iz zbirnih
uzoraka, kori{}enjem 28 prajmera. Prostorna komponenta istra`ivanja, bazira se na
geografskom polo`aju ispitivanih populacija. Ura|ena je analiza prostorne
autokorelacije i interpolacija geneti~kog reljefa. Grupisanje ispitivanih populacija u
regione, kao i njihovo razgrani~enje, izvr{eno je primenom Monmonijerovog
algoritma najve}ih razlika. Za vizualizaciju rezultata i izradu karata regiona
provenijencija bukve u Srbiji, kori{}en je GIS, ~ija je baza podataka obuhvatila
rezultate navedenih istra`ivanja.
Klju~ne re~i: regioni provenijencija, bukva, prostorna analiza geneti~kog diverziteta,
Monmonijerov algoritam, GIS
DELINEATION OF BEECH REGIONS OF PROVENANCES IN
SERBIA BY THE SPATIAL ANALYSIS OF GENETIC DIVERSITY
Results of spatial analysis of genetic diversity have practical application in defining
and delineation of regions of provenances of forest tree species. In this paper, the
main research was conducted on material from 27 natural populations of beech in
Serbia. Genetic component of research is based on RAPD marker analysis of bulked
samples, with use of 28 primers. Spatial component of research was based on geographical position of investigated populations. Genetic and spatial distances were
used for spatial analysis of genetic diversity, with use of spatial autocorrelation, and
interpolation of genetic landscape. Grouping of investigated populations in regions,
together with delineation of these regions, was made by use of Monmonier’s algorithm of maximum differences. Visualization of results and production of maps with
regions of provenances of beech in Serbia was made by using of GIS, which data base
was loaded by results of methods used in this research.
Key words: regions of prov e nances, beech, spa tial anal y sis of genetic di versity,
Monmonier’s algorithm, GIS
80
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VARIJABILNOST LISNE POVR[INE I FIZIOLO[KIH
PARAMETARA KOD TRI PROVENIJENCIJE BUKVE (Fagus sylvatica L.)
U PROVENIJENI^NOM TESTU U SRBIJI
Sr|an Stojni}1, Sa{a Orlovi}1, Andrej Pilipovi}1, Dragica Viloti}2,
Mirjana [ija~i}-Nikoli}2 i Danijela Miljkovi}3
1Institut
3Institut
za nizijsko {umarstvo i `ivotnu sredinu, Novi Sad
2[umarski fakultet, Beograd
za biolo{ka istra`ivanja Sini{a Stankovi}, Beograd
U radu su prikazani rezultati istra`ivanja varijabilnosti lisne povr{ine (LA) i
fiziolo{kih parametara (neto fotosinteza (A), transpiracija (E), stomatalna
provodljivost (gs) i efikasnost kori{}enja vode (WUE)) kod tri provenijencije bukve
(Fagus sylvatica L.) u provenijeni~nom testu osnovanom na Fru{koj gori.
Provenijencije obuhva}ene istra`ivanjem poti~u sa tri lokaliteta koji se prote`u du`
areala bukve od severa prema jugu: Pfalzgrafenweiler (Nema~ka), Grenchen
([vajcarska) i Valkonya (Ma|arska). Rezultati ukazuju da su posmatrani parametri
bili pod uticajem kako stani{nih karakteristika lokaliteta, tako i geneti~ke
konstitucije provenijencija. Na osnovu analize varijanse, konstatovane su statisti~ki
zna~ajne razlike izme|u provenijencija u pogledu prose~ne lisne povr{ine (p£0.05),
intenziteta transpiracije (p£0.05) i efikasnosti kori{}enja vode (p£0.05), dok razlike u
pogledu neto fotosinteze i stomatalne provodljivosti nisu bile statisti~ki zna~ajne.
Jasan klinalan obrazac je konstatovan za redukciju prose~ne lisne povr{ine sa
pove}anjem kserofilnosti stani{ta sa kojeg provenijencije poti~u. Provenijencija koja
poti~e sa najsu{nijeg stani{ta (Valkonya) je imala najmanju lisnu povr{inu i najve}u
efikasnost kori{}enja vode, {to upu}uje na visok potencijal ove provenijencije za
primenu u programima po{umljavanja suvljih stani{ta.
Klju~ne re~i: Fagus sylvatica, provenijeni~ni test, lisna povr{ina, fiziologija
VARIATION IN LEAF AREA AND PHYSIOLOGY AMONG
THREE PROVENANCES OF EUROPEAN BEECH (Fagus sylvatica L.)
IN PROVENANCE TRIAL IN SERBIA
In the paper are presented results of investigation of variability of leaf area (LA) and
physiological parameters (net photosynthesis (A), transpiration (E), stomatal conductance (gs) and water use efficiency (WUE)) of three European beech (Fagus
sylvatica L.) provenances in the provenance trial established on Fruska Gora Mt.
Provenances originate from three localities along a gradient from the north to the
south of Europe: Pfalzgrafenweiler (Germany), Grenchen (Swiss) and Valkonya
(Hungary). Results point to the fact that observed parameters were influenced both
by environmental conditions of sites and genetic constitution of provenances. On the
basis of ANOVA procedure it was observed significant differences among provenances in terms of mean leaf area (p£0.05), rate of transpiration (p£0.05) and water
use efficiency (p£0.05), while differences regarding net photosynthesis and stomatal
conductance were not statistically significant. A broad clinal pattern was recorded for
reduction of mean leaf area with increasing of xeric conditions on locations of provenances origin. Provenance from the driest site (Valkonya) showed the smallest leaf
area and the highest water use efficiency, indicating on high potential for afforestation programmes in more arid areas.
Key words: Fagus sylvatica, provenance trial, leaf area, physiology
81
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA I MORFOLO[KA VARIJABILNOST SMR^E I
DUGLAZIJE U PROVENIJENI^NIM TESTOVIMA U SRBIJI
Vasilije Isajev1, Vera Lavadinovi}2, Milan Mataruga3 i Vladan Iveti}1
3[umarski
1[umarski fakultet, Beograd
2Institut za {umarstvo, Beograd
fakultet, Banja Luka, Bosna i Hercegovina
Uporedna analiza morfometrijskih svojstava 8 provenijencija smr~e [Picea abies (L.)
Karst.] i 24 duglazije [Pseudotsuga menziesii (Mirb.) Franco], obavljena je u pet
monokultura podignutih na razli~itim stani{tima u Srbiji, sa ciljem bli`eg upoznavanja
karaktera njihove geneti~ke, fiziolo{ke i morfolo{ke varijabilnosti. Istar`ivanjima su
dobijene predhodne informacije o proizvodnim sposobnostima pojedinih provenijenica smr~e i duglazije u odre|enim ekolo{kim uslovima i bli`e je upoznata
varijabilnost unutar i izme|u provenijencija. Dosada obavljena, kao i dalja
istra`ivanja, imaju zna~aj pri pouzdanom izboru odgovaraju}ih provenijencija ili
grupe provenijencija za pojedina stani{ta. Osnovane monokulture daljim oplemenjiva~kim programima obezbedi}e postizanje dugoro~nih ciljeva. Predstavljeni
rezultati provenijeni~nih istra`ivanja u radu imaju za cilj i da naglase njihov zna~aj za
unapre|enje proizvodnosti {uma, kao i za {umarsku nauku.
Klju~ne re~i: provenijeni~ni ogledi, smr~a, duglazija, Srbija
GENETICAL AND MORPHOLOGICAL VARIABILITY OF SPRUCE
AND DOUGLAS FIR IN PROVENANCE TESTS IN SERBIA
Com parative anal ysis of morphometric characters of 8 spruce [Picea abies (L.)
Karst.] provenances and 24 Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] were
carried out in five monocultures established on different sites in Serbia, aiming at a
closer study of the character of genetically, physiological and morphological variability. The research provided the preliminary information on the adaptability and productivity of individual Norway spruce and Douglas fir provenances in the juvenile
stage and in the given ecological conditions and a closer study of inter- and intraprovenance variability. All the previous and futures research are significant for a
more reliable choice of the suitable provenances or groups of provenances for the
particular sites. Also, monocultures are used to establish breeding populations, so the
long-term effects will be achieved. This paper presents the results of provenence research in order to emphasize its importance to siviculture, forest production and science.
Key words: provenence experiment, Norway spruce, Douglas fir, Serbia
82
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ PREDSETVENIH TRETMANA NA KLIJAVOST SEMENA
RAZLI^ITIH STABALA TAKSODIJUMA (Taxodium distichum (L.) RICH.)
Vladan Popovi}1, Mirjana [ija~i}-Nikoli}2, Vladan Iveti}2,
Bratislav Matovi}1 i Vera Lavadinovi}1
1Institut za {umarstvo, Beograd
2[umarski fakultet, Beograd
U radu su prikazani rezultati istra`ivanja uticaja ~etiri razli~ita pretsetvena tretmana
na klijavost semena taksodijuma pet stabala poreklom iz semenske planta`e.
Klijavost je ispitivana na standardnom uzorku (4 x 100 semenki po stablu), u trajanju
od 28 dana. Prikupljeni podaci obra|eni su u kompjuterskom programu „Statgraph
6.0“, pri ~emu je prikazana sumarna statistika, dvofaktorijalna analiza varijanse, za
faktore tretman i stablo i LSD-test.
Dobijeni rezultati pokazuju da potapanje semena u rastvor limunske kiseline (100 ili
500 ppm) u trajanju od 48 ~asova, stimuli{e klijavost u laboratorijskim uslovima. Na
osnovu dvofaktorijalne analize dokazano je da su razlike u broju isklijalih semenki
rezultat primenjenih tretmana, a ne porekla semena. Imaju}i u vidu da je ovaj
tretman jednostavan, jeftin i ne{kodljiv po zdravlje ljudi i `ivotnu sredinu mo`e na}i
{iru primenu u proizvodnji sadnica taksodijuma.
Klju~ne re~i: klijavost, seme, tretman, limunska kiselina, taksodijum
EFFECT OF PRE-SAWING TREATMENTS ON SEED
GERMINATION PERCENTAGE OF DIFFERENT TAXODIUM
(Taxodium distichum (L.) RICH.) TREES
The effect of four different pre-sawing treatments on taxodium seed germination percentage was studied on five trees originating from the seed orchard. Germination percentage was researched on a standard sample (4 x 100 seeds per tree), during 28 days.
The study data were processed using the software “Statgraph 6.0“. The summary statistics was presented, the two-way analysis of variance for treatment and tree, and
LSD-test.
The study results show that seed immersion in citric acid (100 or 500 ppm) during 48
hours, stimulates the germination percentage in laboratory conditions. Based on the
two-way analysis of variance, it was confirmed that the differences in the number of
germinated seeds was the result of the applied treatments, and not of the seed origin.
Taking into account that this treatment is simple, inexpensive and harmless to human
health and environment, it can be more widely applied in the production of taxodium
seedlings.
Key words: germination percentage, seed, treatment, citric acid, taxodium
83
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VARIJABILNOST OSOBINA PERSPEKTIVNIH
SELEKCIJA JABUKE GRUPE JONATHAN
Milan Luki}, Sla|ana Mari}, Sanja Radi~evi} i Ivana Gli{i}
Institut za vo}arstvo, ^a~ak
Jabuka je ekonomski najzna~ajnija vrsta roda Malus, koja se u Srbiji gaji na oko
30.000 ha sa prose~nom proizvodnjom od 209.089 t. Oplemenjivanje jabuke zauzima
zna~ajno mesto u programima nau~nih institucija {irom sveta. Sorta Jonathan je jedna
od 4 sorte koje se pojavljuju u pedigreu ekonomski zna~ajnih sorti jabuke. U radu je
prikazano 6 perspektivnih selekcija jabuke, stvorenih u Institutu za vo}arstvo u
^a~ku, kod kojih je Jonathan jedna roditeljska sorta. Ispitivane selekcije su nastale iz
ukr{tanja: Jonathan x Idared (J/3/60 i J/2/53), [Jonathan x J/54/53/59] x J/27/127/62
(J/4/106) i Jonathan x J/54/53/59 (J/1/55, J/1/71 i J/1/15). Prou~avane su fenolo{ke
osobine (fenofaze cvetanja i zrenja), pomolo{ke osobine (fizi~ke i hemijske osobine
ploda) i rodnost. Selekcije nastale ukr{tanjem Jonathan x Idared imale su najve}u
masu ploda (J/2/53; 188,4 g) i najve}i prinos po jedinici povr{ine (J/3/60; 34 t/ha).
Visokim kvalitetom ploda odlikuju se selekcije nastale iz ukr{tanja Jona than x
J/54/53/59. Najve}i sadr`aj rastvorljivih suvih materija imala je selekcija J/1/55
(15,3%), dok je selekcija J/1/71 imala najve}i sadr`aj ukupnih {e}era i kiselina (9,61%
i 1,46%, resp.).
Klju~ne re~i: jabuka, perspektivna selekcija, pomolo{ke osobine
VARIABILITY OF PROPERTIES OF PROMISING
APPLE SELECTIONS OF THE JONATHAN GROUP
Apple is economically the most important species of genus Malus. In Serbia, apple is
cultivated on about 30,000 ha, with an average production of 209,089 t. Apple breeding is carried out on a large scale in many scientific institutes throughout the world.
Jonathan is one of four cultivars, which have been the most frequent progenitors in
apple breeding programmes worldwide. The paper presents 6 promising apple selections bred at Fruit Research Institute, one parental cultivar being Jonathan. The assessed selections have derived from the crosses: Jonathan x Idared (J/3/60; J/2/53),
[Jonathan x J/54/53/59] x J/27/127/62 (J/4/106) and Jonathan x J/54/53/59 (J/1/55;
J/1/71; J/1/15). Some phenological and pomological properties, and cropping have
been assessed. The highest fruit weight (J/2/53; 188,4 g) and yield per unit land area
(J/3/60; 34 t/ha) have been reported in selections derived from the Jonathan x Idared
cross. The selections raised from a cross between Jonathan and J/54/53/59 are characterized by high quality fruits. The highest soluble solids content was found in J/1/55
(15,3%), while the highest total sugars and acids content was evidenced in J/1/71
(9,61%; 1,46%, resp.).
84
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
EFEKTIVNI POLINACIONI PERIOD KLONOVA OBLA^INSKE VI[NJE
Milica Fotiri} Ak{i}1, Vera Rakonjac1, Dragan Nikoli}1,
Sonja Markovi}1, Slavica ^oli}2 i Vlado Li~ina1
2Institut
1Poljoprivredni fakultet, Zemun
za primenu nauke u poljoprivredi, Beograd
Produkcija cvetova i procenat zametanja predstavljaju va`ne osobine u proizvodnji
vi{nje (Prunus cerasus L.), jer sa njihovim pove}anjem raste i prinos. Pored toga, usled
kratke vitalnosti jajne }elije kod nekih sorti vi{nje koja sazreva odmah nakon
otvaranja cvetova, zadovoljavaju}a receptivnost `iga tu~ka, brzo opra{ivanje i brz
prodor polenove cev~ice niz stubi} tu~ka su jako bitni kako bi se osigurala oplodnja
vitalne jajne }elije. U ovom radu ispitivan je efektivni polinacioni period (EPP) kod
~etiri klona Obla~inske vi{nje (II/2, III/9, XI/3 and XIII/1) koji se me|usobno
razlikuju u stepenu klijavosti polena, zametanju plodova i prinosu. Uz pomo}
fuorescentne mikroskopije pra}en je porast polenove cev~ice u fazi balona (-2), u
vreme otvaranja cvetova (0) i 2, 4 i 6 dana nakon opra{ivanja. U isto vreme zametanje
plodova utvr|eno u in vivo uslovima. Zadovoljavaju}e zametanje plodova (> 25%)
kod sva ~etiri klona dobijeno je od nultog do {estog dana nakon opra{ivanja, dok su
najbolji rezultati postignuti ~etvrtog i {estog dana nakon opra{ivanja. Ispitivanja
pokazuju da EPP kod Obla~inske vi{nje traje du`e nego kod ostalih predstavnika
roda Prunus i nekih drugih sorti vi{nje.
Klju~ne re~i: Prunus cerasus, receptivnost `iga, fluorescentna mikroskopija, porast
polenove cev~ice, zametanje ploda
EFFECTIVE POLLINATION PERIOD IN
OBLA^INSKA SOUR CHERRY CLONES
A high flower density and fruit set rate is important in sour cherry (Prunus cerasus L.)
production, because yield increases with higher fruit set ratio. Furthermore, because
of the short life span of the ovule in some sour cherry cultivars that can mature soon
af ter anthesis, sat is fac tory stigma re cep tiv ity, rapid pol li na tion and pol len tube
growth along the style are essential to ensure fertilization of a viable ovule. In this
work, the effective pollination period (EPP) of Obla~inska sour cherry clones (II/2,
III/9, XI/3 and XIII/1) that differ in pollen germination, fruit set and yields was studied. Pollen tube growth during balloon stage (-2), anthesis (0), and 2, 4 and 6 day after
antehsis was done by fluorescent microscopy. At the same time fruit set was determined in vivo. An acceptable fruit set (> 25%) for all four clones was obtained following pollination from day 0 till the day 6, but the best results were recorded in days 4
and 6. Obtained results are showing that EPP in Obla~inska sour cherry last longer
than the rest of the Prunus species and in some other sour cherry cultivars.
Kea words: Prunus cerasus, stigma receptivity, fluorescent microscopy, pollen tube
growth, fruit set
85
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DEKORATIVNE FORME VI[NJE I TRE[NJE
Mirjana Ljubojevi} ,Vladislav Ognjanov, Du{ica Bo{njakovi}, Goran Bara},
Marina Ognjanov, Marija Predojevi} i Jelena ^ukanovi}
Poljoprivredni fakultet, Departman za vo}arstvo, vinogradarstvo,
hortikulturu i pejza`nu arhitekturu, Novi Sad
Prirodne populacije, biodiverzitet, koji se nalazi on farm i introdukovane sorte i selekcije predstavljaju bogat izvor geneti~ke varijabilnosti vi{nje i tre{nje, ali one nikada
nisu bile predmet selekcionog rada sa ciljem stvaranja novih sorti sa dekorativnom
namenom. Selekcijom iz prirodnih populacija bilo je mogu}e izdvojiti slabobujne
forme iako se tre{nja i vi{nja prirodno odlikuju velikom bujno{}u. Slabobujni genotip
vi{nje NS 1/16 KK izdvojen je iz prirodne populacije u Fru{koj Gori, selekcija NS 1/24
KK iz privatnog arboretuma, a selekcija tre{nje NS 6/15 K i R[ 8/27 on farm
o~uvanjem geneti~kih resursa. Smanjenje bujnosti vi{nje i tre{nje postignuto je i
definisanjem posebnih kombinacionih svojstava kao rezultat interakcije podloge i
plemke. Rezultat ovog rada su jedinstvene patuljaste i stubaste forme, koje
objedinjavaju specifi~an geneti~ki potencijal sorte i selekcije, njihove interakcije sa
podlogom i tradicionalno vrtlarsko ume}e. Skupljeni biodiverzitet je jo{ jedna
potvrda da je Balkansko poluostrvo jedan od najvrednijih sekundarnih centara
divergentnosti sa prirodnim resursima kao neiscrpnim genofondom za
oplemenjivanje kako sorti tako i vegetativnih podloga.
Klju~ne re~i: vi{nja, patuljaste forme, Prunus, selekcija, stubaste forme, tre{nja
SWEET AND SOUR CHERRY DECORATIVE FORMS
Biodiversity of natural populations and on farm germplasm of Serbia, introduced
cultivars and selections are a rich source of genetic variability in sour and sweet cherries, but they have never been bread with the aim of creating decorative varieties.
Low vigour - dwarfing and upright - sour cherry genotypes, NS 1/16 KK and NS 1/24
KK, were selected from natural population of Fru{ka Gora and private arboretum,
respectively. Sweet cherry selections NS 6/15 K and R[ 8/27 were part of on farm conservation of genetic resources. Furthermore, reduction in vigour was achieved by defining specific combining abilities as a result of rootstock/scion interaction. The outcome of this study are unique columnar and dwarf forms that integrate specific genetic potential of varieties and selections, their interaction with rootstocks and traditional horticultural skills. Collected biodiversity is another confirmation that the Balkan peninsula is one of the most valuable secondary centres of genetic diversity and
inexhaustible gene pool for breeding both, varieties and vegetative rootstocks.
Key words: decorative forms, Prunus sp., sweet and sour cherry, selection
86
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KI DIVERZITET BUKVE U SRBIJI KAO
OSNOV ZA PROGRAM OPLEMENJIVANJA
Vladan Iveti}1, Ana Nikoli}2 i Vasilije Isajev1
2Institut
1[umarski
fakultet, Beograd
za kukuruz “Zemun Polje”, Beograd
Areal bukve u Srbiji je podeljen na regione provenijencija na osnovu prostorne
analize geneti~kog diverziteta. U cilju daljeg unapre|enja proizvodnje reproduktivnog mate ri jala, po~elo se sa osnivanjem semenskih planta`a za proizvodnju
kvalifikovanog i testiranog (sortnog) materijala. Da bi se na najbolji na~in iskoristio
potencijal vrste, potrebno je bli`e upoznati geneti~ki diverzitet izme|u populacija
bukve. U ovom radu, kori{}enjem RAPD markera ura|eno je grupisanje 27
primernih populacija metodom UPGMA, upotrebom programa TFPGA. Na kraju je
izvr{eno grupisanje ispitivanih populacija po prostornom modelu, upotrebom
programa Geneland. Rezultati primenjenih metoda nisu pokazali grupisanje
ispitivanih populacija bukve po jasnom geografskom obrascu.
Klju~ne re~i: bukva, geneti~ki diverzitet, UPGMA
GENETIC DIVERSITY OF BEECH IN SERBIA
AS BASE FOR BREEDING PROGRAM
Areal OF BEECH IN Serbia is divided on regions of provenances, based on spatial
analysis of genetic diversity. Toward further improvement of production of reproductive material, it was began with establishing of the seed orchards for production of
qualified and tested (sort) material. In order to maximize to the best use of species
potential, understanding of genetic diversity between population is needed. In this
paper, based on the RAPD markers, clustering of 27 sample populations was done by
UPGMA method, using TFPGA computer program. Finally, clustering of researched
populations was done by use of the computer program Geneland. Results of applied
methods didn’t confirmed clustering of beech populations in Serbia by distinct spatial
pattern.
Key words: beech, genetic diversity, UPGMA
87
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ STANI[TA NA [IRINU TRANSFUZIONOG PARANHIMA
PROVENIJENCIJA DUGLAZIJE U TEST KULTURAMA U SRBIJI
Vera Lavadinovi}1, Vasilije Isajev2 i Vladan Popovi}1
1Institut za {umarstvo, Beograd
2[umarski fakultet, Beograd
Jedan od na~ina procene adaptivnosti, varijabilnosti i produktivnosti introdukovanih
vrsta drve}a mogu}e je putem provenijeni~nog testa.
Geneti~ka i ekolo{ka adaptivnost mo`e biti potvr|ena promenljivim morfolo{ko-anatomskim karakteristikama, koji su analizirani u ovom radu.
U Srbiji su osnovana dva provenijeni~na testa sa 20 provenijencija duglazije (Pseudotsuga menziessii /Mirb./ Franco) sa originalnim poreklom semena iz Severne
Amerikena u cilju testiranja vrste.
U cilju upoznavanja efekta interakcije stani{nih uslova lokacija gde su osnovani
provenijeni~ni testove duglazije u Srbiji obavljena je dvofaktorijalna analiza varijanse
{irine transfuzionog parenhima. Prema razultatima anlize varijanse utvr|eno je da
postoje statisti~ki zna~ajne razlike za vrednosti anliziranih morfolo{ko-anatomskih
svojstava ~etina izme|u provenijencija. Test, NZR pokazuje da su provenijencije
homogene, odnosno da razlike u srednjim vrednostima kod njih nisu statisti~ki
zna~ajno razli~ite. Manji opseg variranja svojstava kod ovih provenijencija, u odnosu
na druge, mo`e se uslovno smatrati kao posledica njihove genetske bliskosti koja je i
uslovila sli~ne fenotipske ekspresije u interakciji sa spolja{njim faktorima lokacija gde
su testovi opsnovani.
Klju~ne re~i: Duglazija, provenijencija, morpometrijske karakteristike, transfuzioni
paranhim, Srbija
EFFECT OF HABITAT ON TRANSFUSION PARENCHYMA EXTENT OF
DOUGLAS-FIR PROVENANCES IN TEST PLANTATIONS IN SERBIA
A way to assess adaptability, variability, and productivity of introduced tree species
can be performed by provenance tests.
Genetic and ecological adaptability can be confirmed by variable morphological-anatomical characters, which are the aim of this research. For that reason, two experimental plots with twenty Douglas-fir provenances originating from North America
were established in Southern Serbia.
A two-way analysis of variance was aimed at a closer study of the effect of the interaction of site conditions of Douglas-fir (Pseudotsuga menziessii /Mirb./ Franco) provenance test locations in Serbia on the transfusion parenchyma. The ANOVA results of
the analysed morphological-anatomical characters of needles show that there are statistically significant differences among the provenances. The LSD test shows that the
provenances are homogeneous, i.e. that the differences in their mean values are not
statistically significant. The lower range of character variation in these provenances,
compared to others, can be conditionally considered as a consequence of their genetic
similarity which conditioned the similar phenotype expressions in the interaction with
the external factors of the sites where the tests were established.
Key words: Douglas-fir, provenance test, morphometric characteristics, transfusion
parenchyma, Serbia
88
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OPLEMENJIVANJE JOVE (Alnus sp.) U CILJU FITOREMEDIJACIJE
I ZA PROIZVODNJU BIOMASE ZA ENERGETSKE POTREBE
Vladan Iveti}1, Aleksandra Komarnicki-]irli}2 i Vasilije Isajev1
1[umarski fakultet, Beograd
2JP „Srbija{ume“, Beograd
Jove (crna jova - Alnus glutinosa Gaertn. i bela jova - Alnus incana (L.) Moench.) u {umarstvu Srbije imaju mali zna~aj. Njihov potencijal se ogleda u mogu}nosti osnivanja
zasada kratke ophodnje za potrebe fitoremedijacije i za proizvodnju biomase u cilju
dobijanja energije. Zbog disjunktivnog areala u okviru koga se jove javljaju u
relativno malom broju, selekciona osnova je su`ena. U radu su definisani selekcioni
kriterijumi i dati su rezultati individualne selekcije polaznog materijala. Definisan je
program oplemenjivanja i masovne proizvodnje reproduktivnog materijala sa ciljnim
svojstvima.
Klju~ne re~i: jove, oplemenjivanje, selekcioni kriterijumi, fitoremedijacija, SRF
BREEDING OF ALDERS (Alnus sp.) FOR PHYTOREMEDIATION PURPOSES
AND FOR PRODUCTION OF BIOMASS FOR ENERGETIC PURPOSES
Alders (black alder - Alnus glutinosa Gaertn. and gray al der - Alnus incana (L.)
Moench.) have a small significance in Serbian forestry. Their potential is reflected in
the possibility of short rotation forestry plantations for phytoremediation and for
plantations for energy biomass production. Because of disjunctive areal with relatively small number of individuals in populations, the selection base is narrowed. In
this paper, selection criteria was defined and results of individual selection of based
material was presented. The program of breeding and mass production of reproductive material with target traits is defined.
Key words: alder, breeding, selection criteria, phytoremediation, SRF
89
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
INICIJALNO I FINALNO ZAMETANJE PLODOVA NEKIH
HIBRIDA [LJIVE U ZAVISNOSTI OD TIPA OPRA[IVANJA
Ivana Gli{i}, Radosav Cerovi}, Neboj{a Milo{evi}, Milena \or|evi} i Sanja Radi~evi}
Institut za vo}arstvo, ^a~ak
U radu su prikazani rezultati dvogodi{njih prou~avanja (2009/10) karaktersitika
inicijalnog i finalnog zametanja plodova perspektivnih hibrida {ljive u agroekolo{kim
uslovima ^a~aka u zavisnosti od tipa opra{ivanja. Prou~avani hibridi {ljive su:
38/62/70 (‘Hall’ x ‘California Blue’), IV/63/81 (‘Large Sugar Prune’ x ‘Scoldus’),
22/17/87 (‘^a~anska najbolja’ x ‘Zh’lta Butilcovidna’), 29/29/87 (‘Stanley’ x ‘Scoldus’)
i 34/41/87 (‘Valjevka’ x ‘^a~anska lepotica’). Svaki od navedenih hibrida je ispitivan u
varijanti samoopra{ivanja i slobodnog opra{ivanja. Istovremeno je obavljeno
ispitivanje klijavosti polena in vitro, kao i karakteristike fenofaze cvetanja i obilnosti
cvetanja. Generalno se uo~ava ni`a obilnost cvetanja u toku druge godine prou~avanja. Klijavost polena je varirala od prose~no 25,31% (29/29/87) do 40,01%
(38/62/70). Sa prose~no 31,59% finalno zametnutih plodova u varijanti samoopra{ivanja i 29,38% u varijanti slobodnog opra{ivanja hibrid 34/41/87 je dao najbolje
rezultate, dok se najslabiji rezultati od prose~no 1,61% finalno zametnutih plodova u
varijanti samoopra{ivanja i 7,69% u varijanti slobodnog opra{ivanja bele`e kod
hibrida 32/21/87.
Klju~ne re~i: hibridi {ljive, opra{ivanje, zametanje ploda, klijavost polena
INITIAL AND FINAL FRUIT SET IN SOME PLUM
HYBRIDS UNDER DIFFERENT POLLINATION TYPES
The paper presents the results of the two-year study (2009-2010) of characteristics of
initial and final fruit set in some promising plum hybrids grown in the region of ^a~ak
under different pollination conditions. The following hybrids were studied: 38/62/70
(‘Hall’ x ‘California Blue’), IV/63/81 (‘Large Sugar Prune’ x ‘Scoldus’), 22/17/87
(‘^a~anska Najbolja’ x ‘Zh’lta Butilcovidna’), 29/29/87 (‘Stanley’ x ‘Scoldus’) and
34/41/87 (‘Valjevka’ x ‘^a~anska Lepotica’). Each of the hybrids was studied both under self-pollination and free pollination. In vitro pollen germination was also performed as well as characteristics of flowering phenophase and flowering abundance.
Generally, the results suggest lower flowering abundance in the second year of study.
Pollen germination ranged from averagely 25.31% (29/29/87) to 40.01% (38/62/70).
With averagely 31.59% and 29.38% final fruit set under self-pollination and free pollination variants respectively hybrid 34/41/87 gave the best results, whereas the lowest
per for mance was ob served in hy brid 32/21/87 where pol len ger mi na tion val ues
ranged from averagely 1.61% to 7.69% under self-pollination and free pollination, respectively.
Key words: plum hybrids, pollination, fruit set, pollen germination
90
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
SELEKCIJA AUTOHTONIH GENOTIPOVA VI[NJE
(Prunus cerasus L.) NA PODRU^JU FEKETI]A
Sanja Radi~evi}1, Radosav Cerovi}1, Milan Luki}1, Svetlana Paunovi}1,
Darko Jevremovi}1, Slobodan Milenkovi}2 i Milisav Mitrovi}3
3Visoka
1Institut za vo}arstvo, ^a~ak
2Fakultet za biofarming, Ba~ka Topola
poljoprivredna {kola strukovnih studija, [abac
Autohtoni genotipovi vo}aka su veoma zna~ajan izvor geneti~ke varijabilnosti i
vredan po~etni materijal za oplemenjiva~ki rad. Institut za vo}arstvo u ^a~ku ima
dugu tradiciju prou~avanja autohtonih genotipova kontinentalnih vrsta vo}aka,
sporadi~no o~uvanih i {irenih u nekim lokalitetima na prostoru Srbije. U periodu
2005-2006. godine ispitivane su osobine 9 autohtonih genotipova vi{nje na podru~ju
Feketi}a: vreme cvetanja i zrenja, pomolo{ke osobine, biohemijski sastav ploda i
poljska otpornost prema najzna~ajnijim prouzrokova~ima bolesti vi{nje (Blumeriella
jaapii, Clasterosporium carpophilum, Monilinia sp.). Genotipovi su testirani na
prisustvo Prune dwarf virus i Prunus necrotic ring spot virus. Plodovi ve}ine genotipova
su sazrevali po~etkom druge dekade juna i pripadaju kategoriji krupnih plodova,
izuzetnih organolepti~kih svojstava. Najve}u prose~nu masu ploda imao je genotip
F-1 (8,1 g). Sadr`aj rastvorljivih suvih materija i ukupnih {e}era je bio najvi{i kod
plodova genotipa F-4 (17,60%; 14,25%). Na osnovu kriterijuma poljske otpornosti
prema prouzrokova~ima bolesti vi{nje, mogu se izdvojiti genotipovi F-1, F-2, F-3, F-7
i F-8.
Klju~ne re~i: vi{nja, autohtoni genotipovi
SELECTION OF AUTOCHTHONOUS SOUR CHERRY
(Prunus cerasus L.) GENOTYPES IN FEKETI] REGION
Autochthonous genotypes of fruit species are very important source of genetic variability and valuable material for breeding work. Fruit Research Institute-^a~ak has a
long tradition in studying of autochthonous genotypes of temperate zone fruits, sporadically spread and preserved in some localities in Serbia. In 2005-2006 period, the
properties of nine autochthonous sour cherry genotypes in Feketi} region were investigated: flowering and ripening time, pomological properties, biochemical composition of fruits and field resistance to causal agents of diseases (Blumeriella jaapii,
Clasterosporium carpophilum, Monilinia sp.). The genotypes were tested on the presence of Prune dwarf virus and Prunus necrotic ring spot virus. The majority of genotypes
had the ripening time at the begining of second decade of June and large fruits, with
exceptional organoleptical properties. The highest fruit weight was observed in F-1
genotype (8.1 g). The highest soluble solids and total sugars content was found in F-4
genotype (17.60% and 14.25%, respectively). Based on the criteria of field resistance
to causal agents of diseases, the folowing genotypes were singled out: F-1, F-2, F-3,
F-7 and F-8.
91
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
VARIJABILNOST I KORELACIONA ANALIZA FAKTORA
BIOLOGIJE CVETANJA KOD KLONOVA OBLA^INSKE VI[NJE
Milica Fotiri} Ak{i}, Vera Rakonjac, Dragan Nikoli} i Gordan Zec
Poljoprivredni fakultet, Zemun
Faktori biologije cvetanja koji uti~u na plodono{enje, kao {to su vreme cvetanja,
klijavost polena, broj rodnih gran~ica, broj cvetova i plodova po cm2 popre~nog
preseka debla, broj cvetova po pupoljku, broj zametnutih plodova i efektivnost
prinosa debla, ispitivani su tri godine kod 41 klona Obla~inske vi{nje. Znatna
geneti~ka divergentnost ispoljena je kod ispitivanih klonova i za sve analizirane
osobine utvr|ene su zna~ajne razlike. Ispoljena je i visoka korelacija izme|u nekih
faktora biologije cvetanja. Broj rodnih gran~ica zna~ajno je uticao na broj cvetova,
broj plodova, broj zametnutih plodova i efektivnost prinosa. Tako|e, efektivnost
prinosa je veoma zna~ajno, pozitivno korelisana sa brojem cvetova i plodova po cm2
popre~nog preseka debla, i brojem zametnutih plodova. Zna~ajna ili veoma zna~ajna
negativna korelacija uo~ena je izme|u vremena cvetanja i klijavosti polena, kao i
izme|u broja rodnih gran~ica i efektivnosti prinosa. Tako, klonovi koji kasnije
cvetaju obrazuju ve}i broj rodnih gran~ica, pa samim tim imaju i bolji potencijal za
obrazovanje ve}eg broja cvetova i plodova, nego klonovi koji rano cvetaju. Na
dendrogramu dobijenom primenom hijerarhijske klaster analize izdvojeno je devet
grupa klonova.
Klju~ne re~i: Prunus cerasus, vreme cvetanja, zametanje, rodne gran~ice, efikasnost
prinosa
ANALYSIS OF THE VARIABILITY AND CORRELATIONS OF FLORAL
BIOLOGY FACTORS IN OBLA^INSKA SOUR CHERRY CLONES
Floral biology factors which affect fruiting, such as flowering time, pollen germination, number of fruiting twigs, flowers and fruits per cm2 of trunk cross sectional area,
number of flowers per bud, fruit set and trunk yield efficiency have been studied for 3
consecutive years in 41 Obla~inska sour cherry clones. Substantial genetic divergence
has been found in the set of evaluated clones and significant differences were found
among them in all attributes studied. High correlation existed between some floral biology factors as well. Number of fruiting twigs influenced very significantly flower
number, fruit number, fruit set and yield efficiency. In addition, yield efficiency was
very significant, positively correlated with number of flowers and fruits per cm2 of
trunk cross sectional area and fruit set. Significant or very significant negative correlation was found between flowering time and pollen germination and between number
of fruiting twigs and yield efficiency. Accordingly so, clones that flower later form
higher number of fruiting twigs than early-flowering clones and have higher potential
for flower and fruit production. The divergence between clones determined by hierarchi cal cluster anal y sis indi cated that nine clone groups ex ist on the ob tained
dendrogram.
Key words: Prunus cerasus, pollination time, fruit set, fruiting twigs, yield efficiency
92
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
OSOBINE PLODA I SEMENA GENOTIPOVA DIVLJE TRE[NJE (Prunus
avium L.) NAMENJENIH ZA PROIZVODNJU GENERATIVNIH PODLOGA
Dragan Nikoli}1, Vera Rakonjac1, Milica Fotiri}-Ak{i}1,
Aleksandar Radovi}1 i Jugoslav Trajkovi}2
2Visoka
1Poljoprivredni fakultet, Zemun
poljoprivredno-prehrambena {kola strukovnih studija, Prokuplje
Divlja tre{nja (Prunus avium L.) je najva`nija generativna podloga za tre{nju i vi{nju.
Plod joj je loptastog oblika obi~no crne, crvene ili `ute boje. U ovom radu kod deset
genotipova divlje tre{nje ispitivane su va`nije osobine ploda i semena (boja poko`ice
ploda, masa ploda, masa ko{tice, odvajanje mezokarpa od ko{tice, broj semenki u 1
kg plodova i klijavost semena). Od prou~avanih genotipova jedan je bio sa crnom
bojom poko`ice ploda (D1), pet sa crveno `utom (S1, S3, S4, A1 i D2), tri sa crvenom
(S2, R1 i R3) i jedan sa `utom bojom poko`ice ploda (S5). Najmanju masu ploda i
masu ko{tice (1,34 g; 0,160 g) imao je genotip D1, a najve}u masu ploda i masu ko{tice
(4,17 g; 0,456 g) imao je genotip S3. Kod genotipova S2 i S5 bilo je zastupljeno te{ko
odvajanje mezokarpa od ko{tice, kod genotipova D1 i R3 srednje, a kod ostalih 6
genotipova odvajanje mezokarpa od ko{tice je bilo lako. Broj semenki u 1 kg plodova
varirao je od 2190 (S3) do 6600 (D1). Najve}u klijavost semena imao je genotip D1
(53%), a najmanju genotip R3 (4%).
Kju~ne re~i: divlja tre{nja, plod, seme, generativna podloga
FRUIT AND SEED TRAITS IN WILD SWEET CHERRY (Prunus avium L.)
GENOTYPES INTENDED FOR GENERATIVE ROOTSTOCK PRODUCTION
Wild sweet cherry (Prunus avium L.) is the most important generative rootstock for
sweet and sour cherry. Fruit is oblate with usually black, red or yellow fruit color. Important fruit and seed traits (fruit skin color, fruit weight, stone weight, degree of adherence of stone to flesh, number of seeds per 1 kg of fruits and seed germination) of
ten wild sweet cherry genotypes were analyzed in this study. From examined genotypes one was with blackish skin color (D1), five yellow with blush (S1, S3, S4, A1 and
D2), three with red (S2, R1 and R3) and one with yellow fruit skin color (S5). The
lowest fruit and stone weight (1.34 g; 0.160 g, respectively) had genotype D1, but the
highest (4.17 g; 0.456 g, respectively) showed genotype S3. Genotypes S2 and S5 had
adherent, D1 and R3 showed semi-adherent, while the rest of the six genotypes had
non-adherent stone to flesh. Number of seeds per 1 kg of fruits varied from 2190 (S3)
to 6600 (D1). The highest seed germination showed genotype D1 (53%) while the
lowest genotype R3 (4%).
Key words: wild sweet cherry, fruit, seed, generative rootstock
93
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DIVERZITET I ME\USOBNI ODNOSI KOMPONENTI PRINOSA I
POMOLO[KIH OSOBINA KOD JEDNORODNIH SEJANACA MALINE
Aleksandar Radovi}, Milica Fotiri} Ak{i}, Vera Rakonjac,
Jasminka Milivojevi}, Dragan Nikoli} i Mihailo Nikoli}
Poljoprivredni fakultet, Zemun
U ovom radu ispitivane su komponente prinosa i pomolo{ke osobine kod 20 perspektiv nih jednorodnih sejanaca maline sa crvenom i `utom bojom ploda dobijenih
slobodnom oplodnjom `utog klona sorte Miker. Razli~itost komponenti prinosa i
pomolo{kih osobina kod sejanaca maline dale su mogu}nost stvaranja i
selekcionisanja genotipova pobolj{anih osobina na osnovu superiornog fenotipa.
Uzimaju}i u obzir komponente prinosa, broj rodnih gran~ica po izdanku je ispoljio
visoku korelativnu zavisnost sa svim morfolo{kih osobinama ploda verovatno usled
na~ina selekcionisanja sejanaca od strane oplemenjiva~a. Rezultati multivarijacione
analize ukazuju da prve tri komponente obja{njavaju 73.12% ukupne varijabilnosti
posmatranih osobina. Najve}e vrednosti PC1 odnose se na masu, du`inu i {irinu
ploda, broj rodnih gran~ica po izdanku i sadr`aj saharoze. Kod PC2, najve}e
vrednosti su povezane sa du`inom rodnih gran~ica i brojem cvasti po rodnoj gran~ici.
Raspored sejanaca na grafiku pokazuje geometrijsku udaljenost i prikazuje sli~nost
izme|u genotipova u odnosu sa posmatrane osobine.
Klju~ne re~i: malina, genotip, generativni potencijal, korelacija, multivarijaciona
analiza.
DIVERSITY AND RELATIONSHIPS OF YIELD COMPONENTS AND
POMOLOGICAL TRAITS IN FLORICANE RASPBERRY SEEDLINGS
Yield components and pomological traits of 20 promising floricane raspberry seedlings with red and yellow fruits obtained by open pollination of `Meeker` yellow
clone were investigated in this study. The wide range of diversity in yield components
and pomological traits in floricane raspberry seedlings makes it possible to breed and
select genotypes with improved properties on the basis of superior phenotypes. Regarding generative potential, number of fruiting laterals per cane was highly correlated to all the morphological fruit traits which may be due in part to the method of
seedlings selection by breeders. Results from the principal component analysis indicate that the first three components explained 73.12% of the total variability observed. The highest contribution PC1 corresponded to variable of fruit weight, lenght
and width, number of fruiting laterals per cane and sucrose content. On PC2 the largest scores were due to the length of fruiting laterals and number of inflorescens per
fruiting lateral. The distribution of seedlings on the PC1 and PC2 plot show geometrical distances and reflect similarity among them in terms of variables measured.
Key words: raspberry, genotype, generative potential, correlation, PCA
94
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
STVARANJE CRNIH VINSKIH SORTI ZA
ORGANSKU PROIZVODNJU GRO@\A
Nada Kora}1, Petar Cindri}1, Dragoslav Ivani{evi}1,
Ivan Kuljan~i}1, Mira Medi}1 i Milena Popov2
1Poljoprivredni
fakultet, Departman za vo}arstvo, vinogradarstvo,
hortikulturu i pejza`nu arhitekturu, Novi Sad
2Poljoprivredni fakultet, Departman za fitomedicinu i za{titu `ivotne sredine, Novi Sad
Smanjenje upotrebe pesticida u vinogradarskoj proizvodnji u cilju o~uvanja `ivotne
sredine i ljudskog zdravlja je mogu}e gajenjem sorti visoko tolerantnih ili otpornih na
gljivi~ne bolesti. Na Oglednom dobru Poljoprivrednog fakulteta iz Novog Sada u
Sremskim Karlovcima u toku poslednje tri decenije realizovana su ~etiri ciklusa
interspecies ukr{tanja sa ciljem stvaranja sorti dobrog kvaliteta i visoke otpornosti na
faktore stresa.. Iz kombinacije ukr{tanja sorti Cabernet Franc klon E 11 i Panonia,
odabrani su najbolji genotipovi, kandidati za nove crne vinske sorte. Odabrani
kandidati SK 01-1 i SK 01-11, su ispoljili visok stepen otpornosti prema gljivi~nim
bolestima, dobru rodnost, otpornost na mrazeve i odli~an kvalitet vina.
Klju~ne re~i: vinova loza, sorta, kvalitet, otpornost
BREEDING OF BLACK WINE GRAPE CULTIVARS
FOR ORGANIC PRODUCTION
Reducing pesticide use in vineyard production in order to preserve the environment
and human health is posible by growing cultivars tolerant or resistant to fungal diseases. At the Experimental station in Sremski Karlovci during last three decades four
cycles of successive backcrossings were made giving emphases on quality improvement combined with resistance to the main fungal diseases. From a crossing between
Cabernet Franc clone E 11 and Pannonia, the best genotypes were selected. Genotypes SK 01-1 and SK 01-11, demonstrated a high degree of tolerance to fungal diseases, a good yield, resistance to frost and excellent quality wines.
Key words: grapes, cultivar, quality, resistance
95
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
SUBKLONOVI RIZLINGA ITALIJANSKOG
Dragoslav Ivani{evi}, Nada Kora}, Petar Cindri},
\or|e Papri}, Ivan Kuljan~i} i Mira Medi}
Poljoprivredni fakultet, Departman za vo}arstvo, vinogradarstvo,
hortikulturu i pejza`nu arhitekturu, Novi Sad
Klonska selekcija je na~in da se elemini{e negativni uticaj mutacionih promena u
budu}em vinogradu, kao i da se spre~i razmno`avanje biljaka koje su zara`ene
virusima ili virusima sli~nim bolestima. Sorta Rizling italijanski je stara sorta. U
Vojvodini se gaji ve} vi{e vekova. Klonska selekcija sorte Rizling italijanski u
Sremskim Karlovcima je otpo~ela 1975. godine. U januaru 1991. priznata su tri klona
ove sorte. Ubrzo nakon toga je otpo~eo rad na subklonskoj selekciji ova tri klona.
Ovaj rad predstavlja rezultate subklonske selekcije sorte Rizling italijanski.
Subklonovi SK 54-4 i SK 54-10 se karakteri{u nadprose~nim kvaliteetom, i njihovo
gro`|e se, u ve}ini godina, mo`e koristiti za proizvodnju vrhunskih vina.
Klju~ne re~i: Rizling italijanski, vinske sorte, klonska selekcija
RIESLING ITALICO SUBCLONES
Clone selection in viticulture represents a way of eliminating negative influence of
mutation changes in the future vineyard, as well as preventing the virus-infected
plants and plants infected with virus-related diseases from further propagation. Riesling Italico sort is an old sort. It has been grown in Vojvodina for centuries. Clone selection of Riesling Italico sort in Sremski Karlovci was started in 1975. In January
1991. three clones were recognized. Shortly after, the subclone selection of these
three clones started. This paper present resultants of subclone selection of Riesling
Italico. Subclones SK 54-4 and SK 54-10 are characterised by outstanding quality, and
the grapes of these subclones can be used for the production of high quality wines in
most of the years.
Key words: Riesling Italico, wine grape, clonal selection
96
Sekcija / Section 5
Oplemenjivanje `ivotinja
i mikroorganizama
Breeding of animals
and microorganisms
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KOMPARACIJA KOEFICIJENATA NASLEDNOSTI PROIZVODNIH
OSOBINA U POPULACIJI KRAVA CRNO BELE RASE
Zvonko Spasi}, Bo`idar Milo{evi}, Neboj{a Lali},
Sonja Samard`i}, Zoran Ili} i Bojana Ristanovi}
Poljoprivredni fakultet, Le{ak
Na osnovu genetske varijabilnosti ekonomski zna~ajnih osobina opredeljujemo se za
odre|ene metode u genetskom unapre|enju zapata. Me|utim, heritabilitet (h2) je
zna~ajan i za odgovor na pitanje koje se osobine mogu uklju~iti u ocenu priplodne
vrednosti grla.
Za utvr|ivanje heritabiliteta je posmatrano 1.502 grla crno bele rase: 751 majka i 751
k}er (ukupno 6.393 laktacije).
Koeficijenti naslednosti ispitivanih osobina krava izra~unati su primenom tri metode:
regresijom k}eri na majke, intraklasnom korelacijom po bikovima-o~evima i za one
osobine kod kojih postoji ponavljanje u toku `ivota metodom repitabiliteta (R).
Za prose~an prinos mleka, mle~ne masti i mast korigovanog mleka tokom
standardnih laktacija metodom regresije i repitabilitetom, ustanovljeni su srednji
koeficijenti naslednosti (od 0,291 do 0,323), dok su intraklasnom korelacijom
ustanovljeni visoki koeficijenti (od 0,461 do 0,543).
Koeficijenti heritabiliteta za prose~an sadr`aj mle~ne masti su niski (od 0,029 do
0,192) pa je, prema tome, dalje pobolj{anje ove kvantitativne osobine u populaciji
mogu}e preko o~eva-bikova sa ve}im genetskim potencijalom.
Klju~ne re~i: heritabilitet, repitabilitet, crno bela rasa
COMPARATION OF HEREDITY COEFFICIENTS OF PRODUCTIVE TRAITS
IN THE POPULATION OF BLACK WHITE COWS
On the basis of genetic variability of economically important traits we orientate ourselves for specific methods in genetic improvement of a population. However, the
heritability (h2) is important for giving the answer, which traits can be included in the
breeding value evaluation of a population.
For determining the heritability 1502 black white breed cows were used: 751 mothers
and 751 daughters (together 6393 lactations).
The heritability coefficient of investigated traits in cows was calculated using three
methods: regression of daughters to mothers, interclass correlation according to
bulls-sires and, for those features for which recurrence exists during the life we used
method of repeatability (R).
For average milk yield, milking fat and fat edited milk during standard lactations by
the method of regression and repeatability intermediate coefficients of heredity were
determined (from 0,291 to 0,323), while by the interclass correlation were determined
high coefficients (from 0,461 to 0,543).
The heritability coefficients for average content of milking fat were low (from 0,029
to 0,192), thus further improvement of this quantitative trait in the population would
be possible mainly via bulls sires with the higher genetic potential.
Key words: heritability, repeatability, black white breed
98
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
HERITABILNOST PROIZVODNIH OSOBINA REGIONALNIH
POPULACIJA MEDONOSNE P^ELE IZ SRBIJE
Goran Jevti}, Bojan An|elkovi}, Zoran Lugi}, Bora Dini} i Sne`ana An|elkovi}
Institut za krmno bilje, Kru{evac
U ogled je uklju~eno {est regionalnih populacija medonosne p~ele iz rasinskog,
pe{terskog, moravskog, banatskog, timo~kog i kopaoni~kog regiona. Za svaku populaciju pra}ene su dve generacije (maj~inska i potomci). Zbog specifi~nosti pona{anja i
razvoja p~elinjih dru{tava, pra}ene su proizvodne osobine u dva pregleda (prole}ni i
jesenji) za svaku godinu ogleda. Ogled je vr{en u nekontrolisanim uslovima
sparivanja matica (slobodno sparivanje) u cilju utvr|ivanja nasledljivosti proizvodnih
osobina u uslovima prakti~nog p~elarenja. Posmatrane su proizvodne karakteristike i
izra~unati su koeficijenti heritabilnosti. Prinos meda je uslovljen i geneti~kim
faktorima, ali i uslovima spolja{nje sredine, pa je heritabilnost varirala od 72.72%
(rasinski region) do 81.04% (banatski region). Rojevni nagon je zavisio od spolja{njih
uslova (10.81% kod banatske populacije i 33.33% kod rasinske, moravske i timo~ke
populacije). Najvi{i koeficijent heritabilnosti za tem per a ment je utvr|en kod
moravskih p~ela, a najmanji kod kopaoni~kih p~ela. Sli~ni rezultati su i za grabe`
(51.12% kod moravskih i 32.54% kod kopaoni~kih p~ela). Koli~ina p~ela je dosta
zavisila od spolja{njih faktora, ali i od samih populacija. Sli~no je i sa koli~inom legla.
Za koli~inu meda i polena su dobijene su velike razlike u vrednostima koeficijenata
heritabilnosti, sto se obja{njava spregom geneti~kih faktora (nagon za sakupljanjem) i
faktora sredine (medonosno bilje).
Klju~ne re~i: medonosna p~ela, heritabilnost, proizvodne osobine
HERITABILITY OF PRODUCTION CHARACTERISTICS OF
REGIONAL HONEY BEE POPULATIONS FROM SERBIA
Six regional populations of honey bees were included in this study (Rasina, Pe{ter,
Morava, Banat, Timok and Kopaonik region). Two generations (maternal and offspring) were studied for each regional population. Due to the specific behavior and
development of honey bee colonies, production traits were observed in two surveys
(spring and fall) for each year of the research. The experiment was performed in uncontrolled conditions, meaning there was free mating of queen bees and all in order
to determine heritability of production traits in terms of practical beekeeping. Production traits were studied and the heritability coefficients for those traits were calculated. The yield is conditioned by both genetic factors and environmental conditions
and heritability ranged from 72.72% (Rasina region) to 81.04% (Banat region).
Swarming instinct is very dependent on external conditions (10.81% for the bees from
Banat, 33.33% for bees from Rasina, Morava and Timok re gion). The high est
heritability coefficients for temperament were determined in bees from the Morava
region, and the lowest in bees from the Kopaonik region. Similar results were for
heritability coefficients for honey robbing (51.12% for bees from Morava region and
32.54% for bees from Kopaonik region). The amount of bees in the spring survey was
much dependent on external factors, but also from the very populations. Similar was
for the amount of brood. There were large differences in heritability coefficients for
the amount of honey and pollen, which can be explained by the interactions of genetic
factors (the foraging urge) and environmental factors (nectar flora).
Key words: honey bee, heritability, regional populations
99
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UGLOVI KRILNE NERVATURE I LINEARNA RASTOJANJA KOD
MEDONOSNE P^ELE U FUNKCIJI O^UVANJA BIODIVERZITETA SA
PODRU^JA JABLANI^KOG OKRUGA
Mi}a Mladenovi}1,Valentina Pe{eva2 Bojan An|elkovi}3 i Sla|an Ra{i}1
1Poljoprivredni fakultet, Zemun
2Poljoprivredni fakultet, Le{ak
3Institut za krmno bilje Kru{evac
Pod pojmom biodiverzitet ili biolo{ka raznovrsnost u slu~aju medonosne p~ele
podrazumeva se raznolikost i sveukupnost genotipskih i fenotipskih varjeteta
medonosne p~ele. To uklju~uje i hromozomski, morfometrijski i specifi~ni diverzitet,
kako na intra-tako i na ekstrapopulacijskom nivou.
Cilj rada je utvr|ivanje morfometrijskih parametara uglova izme|u nerava na
prednjem krilu i to: A4, B4, D7, E9, G18, J10, J16, K19, L13, N23 i O26 kao i
analiziranje morfometrijskih parametara na zadnjem krilu medonosne p~ele i to
uglova W1, W2 i W3 i linearna rastojanja na zadnjem desnom krilu: L1, L2, L3, L4,
L5, L6, L7 i L8. Tako|e su utvr|eni i koeficijenti heritabilnosti za navedene osobine.
Uzorci p~ela uzeti su sa podru~ja Jablani~kog Okruga i to lokaliteta Slavujevce, Strojkov ce i Leskovac. Uzorci su sa~injeni od po 10 p~ela i predstavljaju autohtoni
materijal.
U radu je utvr|ena i ukupna varijabilnost ispitanih uzoraka.
Klju~ne re~i: medonosna p~ela, morfometrija, heritabilnost, varijabilnost
ANGLES OF WING NERVATURE AND LINEAR DISTANCES
IN HONEY BEE IN FUNCTION OF PERSERVING BIODIVERSITY
OF JABLANICA DISTRICT
The term biodiversity, in the case of honey bee, involves diversity and totality of
geno typic and phenotypic vari et ies of honey bee. It includes chro mo somal,
morphometric and specific diversity, both intra- and the extrapopulation level.
Aim of this study was to determine the morphometric parameters of the angles between the nerves on the front wing. Next angles were determined: A4, B4, D7, E9,
G18, J10, J16, K19, L13, N23 and O26. Also, the analyses of morphometric parameters on the rear wing (angles W1, W2 and W3) and the linear distances on the rear
right wing: L1, L2, L3, L4, L5, L6, L7 and L8 were conducted. The heritability coefficients for these traits were also determined.
Honey bee samples were taken from the area of Jablanica District, sites Slavujevac,
Strojkovce and Leskovac. The samples were composed of 10 honey bees and represented indigenous material.
The total variability of tested samples was determined.
Key words: honey bee, morphometry, heritability, variability
100
Sekcija / Section 6
Biotehnologija u oplemenjivanju
bilja, `ivotinja i mikroorganizama
Biotechnology in breeding of plants,
animals and microorganisms
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
MOLEKULARNA KARAKTERIZACIJA
GENETI^KIH RESURSA JE^MA IZ SRBIJE
Gordana [urlan-Momirovi}1, Dragan Perovi}2, Frank Ordon2, Miroslav Zori}3,
Gordana Brankovi}1, Irena ]ali}1, Kamenko Bratkovi}4 i Vesna Kandi}5
2Julius
1Poljoprivredni fakultet, Zemun
Kuehn-Institute (JKI), Nacionalni istra`iva~ki centar za kulturne biljke, Institut za
istra`ivanje otpornosti i tolerantnosti na stres, Quedlinburg, Nema~ka
3Tehnolo{ki fakultet, Novi Sad
4Centar za strna `ita, Kragujevac
5Institut za kukuruz “Zemun Polje”, Beograd
Diverzitet 144 genotipova je~ma iz Gen banke Srbije je procenjen kori{}enjem
jednostavnih repetativnih sekvenci (SSR) i morfolo{kih markera, radi identifikovanja
novih izvora varijabilnosti koji mogu ubrzati razvoj novih sorata je~ma u Srbiji.
Poreklo genotipova je iz razli~itih oplemenjiva~kih programa biv{e Jugoslavije,
Meksika, Japana, Holandije i Nema~ke. Petnaest SSR markera, sa svih 7 hromozoma
diploidnog genoma je~ma, sa 1-2 SSR markera po hromozomu, sa opsegom od 3-18
alela po lokusu, je kori{}eno za karakterizaciju geneti~kog diverziteta genotipova.
Ukupno 15 lokusa i 113 alela je detektovano, sa prosekom od 7.53 alela po lokusu.
PIC vrednosti po lokusu su pokazale pozitivnu korelaciju sa sa brojem alela po lokusu
za sve genotipove (r = 0.623, P < 0.01). Ukupan broj alela (85) i prose~an broj alela po
lokusu (6.67) su bili najve}i za grupu ozimog je~ma, i sli~ni vrednostima u grupi
{estoredog je~ma (79; 5.93). Izme|u tri grupe razli~itih formi rasta, geneti~ki
diverzitet je bio najve}i za ozime (0.612) i {estorede (0.611) genotipove, nego za
ozime i dvorede (0.322; 0.47) genotipove.
Klju~ne re~i: je~am, geneti~ki diverzitet, fenotipske osobine, SSR markeri
MOLECULAR CHARACTERISATION OF BARLEY
GENETIC RESOURCES FROM SERBIA
The diversity of 144 barley accessions from Serbian Gen Bank was assessed by simple
sequence repeats (SSR) markers and by morphological traits, to identify new sources
of variability that could accelerate the development of improved barley varieties in
Serbia. The origin of accessions is from different breeding programs of former Yugoslavia, Mexico, Japan, Holland and Germany. Fifteen SSR markers, covering all 7
chromosomes of the diploid barley genome with 1-2 SSR markers per chromosome,
with a range of 3-18 alleles per locus, were used to characterize the genetic diversity of
accessions. In total, 15 loci and 113 alleles were detected, with an average of 7.53 alleles per locus. The PIC per locus showed a positive correlation with the number of alleles per locus for all genotypes (r = 0.623, P < 0.01). Total number of alleles (85) and
mean alleles per locus (6.67) were the highest for the winter barley group and were
similar to those in the 6-row group barley (79; 5.93, respectively). Among the three
growth habit groups, gene diversity was higher for the winter (0.612) and six-row accessions (0.611), than for the winter and two-row accessions (0.322; 0.47, respectively).
Key words: barley, genetic diversity, phenotypic traits, SSR markers
102
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DINAMIKA GENETI^KE VARIJABILNOSTI GLIJADINSKIH
LOKUSA KOD SORTI OZIME P[ENICE Triticum aestivum L.
STVORENIH U DUGOGODI[NJEM NAU^NOM OPLEMENJIVANJU
Aleksandra Yurievna Dragovi}1, Desimir Kne`evi}2 i Alexander M. Kudryavtsev1
1Institut
op{te genetike N.I. Vavilova Ruske Akademije Nauka, Moskva, Rusija
2Poljoprivredni fakultet, Le{ak
Izu~ena je promenljivost sorti meke p{enice prema multiplim alelima glijadinkodiraju}ih lokusa, registrovanih elektroforezom. Izu~avane su skoro sve sorte, stvorene u
20. i po~etkom 21. veka u 4 regiona Evrope: Ruska Federacija - Moskovska oblast (56
sorti), Ruska Federacija - Krasnodarski kraj (80 sorti), Srbija (118 sorti) i Italija (129
sorti). Cilj je bio da se izu~i dinamika geneti~ke promenljivosti meke p{enice u
uslovima empirijske selekcije. Ustanovljeno je da u Moskovskoj oblasti u toku 77
godina selekcije broj alela se smanjio 2 puta: izgubljeno je 40 alela, koji su se nalazili
kod starih sorti, pojavilo se samo 10 novih alela za region, a kod 70% savremenih sorti
dominiraju aleli od sorte Mironovskaja 808.
Indikatori geneti~ke varijabilnosti kod sorti Krasnodarskog kraja, Srbije i Italije,
prakti~no se nisu menjali tokom vremena. Ali, izra`en je proces su`avanja geneti~ke
promenljivosti sorti ovih regiona kao rezultat i{~ezavanja alela, karakteristi~nih za
stare sorte i njihovom zamenom sa alelima, koji su prisutni kod nekoliko izvanrednih
sorti, koje su kori{}ene kao donori u sva tri centra. Generalno je nadjeno da kako u
prostoru tako i vremenski pod uticajem empirijske selekcije se smanjuje geneti~ka
varijabilnost vrste.
Klju~ne re~i: p{enica, sorte, varijabilnost, aleli, glijadini
DYNAMICS OF GENETIC VARIABILITY IN GLIADIN LOCI
AMONG THE WINTER WHEAT CULTIVARS Triticum aestivum L.
DEVELOPED IN A LONG TERM SCIENTIFIC BREEDING
The variability of common wheat cultivars according to multiple alleles of gliadincoding loci registered by electrophoresis, was studied. The majority of cultivars developed during 20th and the beginning of 21st century in four regions of Europe: Russian
Federation - Moscow region (56 cultivars), Russian Federation - Krasnodar region
(80 cultivars), Serbia (118 cultivars) and Italy (129 cultivars) was studied. The goal
was to learn dynamics of genetic variability of common wheat in terms of artificial selection. It was found that in Moscow region during 77 years of selection the number of
alleles decreased 2 times: 40 alleles found in old cultivars were lost, only 10 new alleles appeared, and alleles of Mironovskaya 808 dominate in modern cultivars (70%).
Genetic variation indicator in cultivars from Krasnodar, Serbia and Italy, practically
does not change during long term breeding. However, the increasing similarity of the
genetic variation of wheat cultivars of the regions and decreasing of genetic distance
between them was shown. It was caused mainly by replacement of old cultivar alleles
with new ones, characteristic for some excellent cultivars using as donors in all 3 regions.
Generally it has been shown that both in space and time there is decreasing of species
genetic diversity due to long term artificial selection.
Key words: wheat, cultivar, variability, alleles, gliadins
103
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
IDENTIFIKACIJA I VALIDACIJA MAJOR QTL-A ZA
KONCENTRACIJU KADMIJA U LISTU KUKURUZA
Domagoj [imi}1, Hrvoje Fulgosi2, Roberta Sori}3, Zdenko Lon~ari}3, Tatjana
Leden~an1, Antun Jambrovi}1, Zvonimir Zduni}1, Vlado Kova~evi}3 i Ivan Brki}1
1Agricultural Institute Osijek, Osijek, Croatia
2Ru|er Bo{kovi} Institute, Zagreb, Croatia
3J.J. Strossmayer University, Osijek, Croatia
Kadmij (Cd) je te{ki metal toksi~an za ljude, `ivotinje i biljke pri vrlo malim koncentracijama. Mobilnost kadmija i njegova dostupnost ratarskim kulturama predstavlja
rizik za prehrambeni lanac. Pobolj{ani genotipovi kukuruza s u~inkovitom akumulacijom kadmija mogu imati va`nu ulogu u tretiranju tla koje je njime kontaminirano.
U prija{njem istra`ivanju na mapiraju}oj populaciji kukuruza B84×Os6-2
identificirali smo jedan major lokus kvantitativnog svojstva (QTL) za koncentraciju
Cd u listu na kromosomu 2 (bin 2.06). LOD vrijednost od 32.5 ukazuje na veliku
vjerojatnost da taj QTL presudno utje~e na koli~inu Cd u listu. Kako bismo validirali
ovaj podatak, napravili smo QTL analizu koja je uklju~ila 207 rekombinantnih inbred
linija kukuruza IBM populacije (me|ukri`ane B73×Mo17) i 636 molekularnih
markera. Rezultati na ovoj populaciji su potvrdili prija{nje rezultate o postojanju major QTL-a za akumulaciju Cd u listu s LOD vrijednosti od 20.0 na istoj poziciji na binu
2.06. Detektirali smo potencijalni kandidatni gen poznate funkcije na osnovi
kolokacije s major QTL-om. Na{i rezultati bi mogli pomo}i pri brzom razvoju
genotipova kukuruza sa smanjenom/pove}anom akumulacijom kadmija u listu
manipulacijom toga gena. Uporaba detektiranog gena u oplemenjivanju kukuruza
pomo}u novoga cisgenog pristupa bit }e raspravljena.
Klju~ne rije~i: kadmij, cisgeneza, list, kukuruz, QTL
IDENTIFICATION AND VALIDATION OF A MAJOR QTL
AFFECTING LEAF CADMIUM CONCENTRATION IN MAIZE
Cadmium (Cd) is heavy metal toxic to humans, animals and plants at very low concentrations. Mobility and availability of Cd to crop plants pose risk to the food chain.
Improved maize genotypes with effective ability to accumulate Cd can play an important role in treating Cd-contaminated soil. Our previous study conducted on a maize
mapping population B84×Os6-2 identified a major quantitative trait locus (QTL) for
leaf Cd concentration on chromosome 2 (bin 2.06). The LOD score for the QTL of
32.5 indicated very high probability that this QTL strongly affected amounts of Cd in
leaves. In order to validate this QTL, we analyzed 207 recombinant inbred lines of
intermated B73×Mo17 (IBM) population with 636 molecular markers. Our results
validate the presence of a major QTL for leaf Cd accumulation with the LOD score of
20.0 at the same po sition on bin 2.06. We detected a putative candidate gene of
known function on the basis of co-location with the major QTL. Our findings could
aid rapid development of maize genotypes with decreased/increased Cd accumulation
in leaves by manipulation of the gene. Using the detected gene in maize breeding via
the new cisgenic approach will be discussed.
Key words: cadmium, cisgenesis, leaf, maize, QTL
104
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UPOTREBA MOLEKULARNIH MARKERA U SELEKCIJI
NA OTPORNOST PREMA Puccinia triticina
Jelena Bo{kovi}, @eljana Priji} i Veselinka Ze~evi}
Fakultet za biofarming, Ba~ka Topola
Prouzrokova~ lisne r|e Puccinia triticina je jedna od nazna~ajnih bolesti p{enice u
svetu. U veoma kratkom vremenskom intervalu mo`e prouzrokovati velike gubitke u
prinosu. Geneti~ka otpornost sorti je siguran metod za obezbe|ivanje visokih i
stabilnih prinosa. Otkriveno je preko 60 gene na otpornosti prema prouzrokova~u
lisne (Lr geni) r|e, a samo deo njih je izolovan i mapirano, to su: Lr 1, Lr 9, Lr 10, Lr
19, Lr 20, Lr 21, Lr 24, Lr 25, Lr 26, Lr 28, Lr 29, Lr 35, Lr 34, Lr 37, Lr 47, Lr 49 i Lr 51.
Ve}ina gena otpornosti su rasno specifi~ni i odgovaraju konceptu gene za gen.
Klju~ne re~i: Puccinia triticina, molekularni markeri, otpornost
USE OF MOLECULAR MARKERS IN BREEDING
FOR RESISTANCE TO Puccinia triticina
Leaf rust caused by Puccinia triticina is one of the most important wheat diseases
worldwide. It can cause huge and serious yield losses in short time. Genetic resistance
is the preferred method to reduce losses from leaf rust, and 60 leaf rust resistance (Lr)
genes have been designated in wheat. In recently few years it made important progress for application molecular markers in breeding programs on different plants.
Among the Lr genes, Lr1, Lr 9,Lr10, Lr 19, Lr 20, Lr 21, Lr 24, Lr 25, Lr 26, Lr 28, Lr
29, Lr 35, Lr 37, Lr 47, Lr 49 Lr 51 and Lr 34 were isolated and characterized through a
map-based cloning approach. Most Lr genes confer race-specific resistance in a
gene-for-gene manner.
Key words: Puccinia triticina, molecular markers, resistance
105
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA KARAKTERIZACIJA PROUZROKOVA^A
CRVENE PRSTENASTE PEGAVOSTI BORA Dothistroma sp. PUTEM
MOLEKULARNIH METODA
Vladislava Galovi}1, Sa{a Orlovi}1, Bojana Kla{nja1,
Predrag Pap1, Branislav Trudi}1 i Miroslav Zori}2
1Institut
za nizijsko {umarstvo i `ivotnu sredinu, Novi Sad
2Poljoprivredni fakultet, Zemun
Dothistroma septosporum je prouzrokova~ crvene prstenaste pegavosti ~etinara i poznato je da prouzrokuje zna~ajne gubitke u planta`ama i {umama crnog bora
zara`avaju}i vi{e od 60 razli~itih vrsta borova sa izra`enom kosmopolitskom
distribucijom. Iako literaturni navodi ukazuju na postojanje dve vrste ovog patogena,
do sada nema dokaza o postojanju dva razli~ita varijeteta Dothistroma sp. u zasadima
crnog bora u Srbiji. Poznavanje ta~ne taksonomske pripadnosti je zna~ajno za
kreiranje i sprovo|enje programa oplemenjivanja i suzbijanja patogena, a naro~ito
zbog globalnih klimatskih promena. Cilj ove studije bio je da se primenom
molekularnih markera genetski karakteri{e vrsta ovog patogena da bi se redukovao
njegov uticaj u regionalnim {umama i planta`ama bora. U ovom radu kori{}eno je
{est izolata sa razli~itih lokacija u Srbiji. Genomska DNA je izolovana iz “in vitro”
kulture ili “in planta” iz inficiranih iglica crnog bora. Za molekularnu identifikaciju
patogena primenjeno je nekoliko metoda: SSR, ITS - PCR, ITS - RFLP. Nakon
obrade podataka dobijenih molekularnim metodama utvrdjeno je da pathogen koji
izaziva crvenu prstenastu pegavost bora u Srbiji pripada vrsti Dothistroma septosporum
var. lineare.
Klju~ne re~i: cni bor (Pinus nigra), Dothistroma sp., molekularna karakterizacija
GENETIC CHARACTERIZATION OF THE PINUS NEEDLE BLIGHT
PATHOGEN Dothistroma sp. BY MOLECULAR METHODS
Dothistroma septosporum cause the red band needle blight and is a most damaging
pathogen known to infect over 60 different pine species with its cosmopolitan distribution and thus causes severe losses in pine plantations and forests worldwide. Although the literature proved the existence of two different varieties of Dothistroma
sp., until now there was no certain prove of existence of two different varieties of the
pathogen in pine plantations in Serbia. Knowing the exact taxonomic affiliation is important for the creation and implementation of breeding programs and the prevention of pathogens, especially due to global change. The aim of this study was to apply
molecular techniques to genetically characterize the variety of this pathogen in order
to reduce its negative impact on regional pine forests and plantations. Six isolates
from various locations in Serbia were taken. Their genomic DNA were extracted either from “in vitro” culture or “in planta” from infected needles of Pinus nigra. Several
molecular methods were applied: SSR, ITS-PCR based, ITS-RFLP. After processing
the molecular data the Dothistroma septosporum var. lineare was determined.
Key words: Austrian pine (Pinus nigra), Dothistroma sp., molecular characterization
106
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETSKI MODIFIKOVANO DRVE]E - STANJE I PERSPEKTIVE
Marina Noni}1, Cristina Vettori2, Fabio Boscaleri3,
Jelena Milovanovi}4 i Mirjana [ija~i}-Nikoli}1
1[umarski fakultet, Beograd
za genetiku biljaka, Nacionalni istra`iva~ki savet u Firenci, Italija
3Generalna direkcija regiona Toskana, Sektor za razvoj poljoprivrednih i prehrambenih
preduze}a, Firenca, Italija
4Fakultet za primenjenu ekologiju “Futura”, Beograd
2Institut
Genetski modifikovano drve}e je rezultat savremenog oplemenjivanja biljaka.
Njegovo uvo|enje u `ivotnu sredinu u eksperimentalne svrhe ili {ire gajenje
definisano je razli~ito, od zemlje do zemlje. Mi{ljenje javnosti je podeljeno!
Obavljena istra`ivanja deo su aktivnosti u okviru COST akcije FP0905 „Biosafety of
forest transgenic trees”, koja ima za cilj prikupljanje informacija i definisanje nau~nog
stava o genetski modifikovanom drve}u kao osnove za budu}u politiku Evropske
unije u toj oblasti. Prikupljene informacije odnose se na osam zemalja: ~etiri zemlje
~lanice Evropske unije (Italija, Slovenija, Rumunija i Bugarska) i ~etiri zemlje u
procesu pridru`ivanja (Hrvatska, Crna Gora, Srbija i Bosna i Hercegovina).
Komparativnom analizom obuhva}eno je stanje {umskih resursa (povr{ina {umskog
zemlji{ta, {umski pokriva~, vlasni{tvo nad {umama, tip {uma), zakonodavstva vezano
za {umarstvo i za genetski modifikovane organizme i stav {ire javnosti o ovom
pitanju.
Prikupljene informacije pru`aju dobru osnovu za sagledavanje ove problematike u
cilju definisanja jasnog nau~nog stava kao preporuke.
Klju~ne re~i: genetski modifikovano drve}e, {umski resursi, zakonska regulativa, stav
javnosti
GENETICALLY MODIFIED TREES - STATE AND PERSPECTIVES
Genetically modified trees are the result of modern plant breeding. Its introduction
into the environment for experimental purposes or wider cultivation is defined differently from country to country. Public opinion is divided!
Con ducted re search are part of the ac tiv i ties within the COST Ac tion FP0905
„Biosafety of forest transgenic trees”, which aims to collect information and define the
scientific attitude on genetically modified trees as a basis for future European Union
(EU) policy in this field. The collected information refer to eight countries: four EU
member states (Italy, Slovenia, Romania and Bulgaria) and four countries in the process of joining EU (Croatia, Montenegro, Serbia and Bosnia and Herzegovina). A
comparative analysis involved the state of forest resources (area of forest land, forest
cover, forest ownership, forest type), forestry legislation, legislation relating to genetically modified organisms and the general public attitude on this issue.
The collected information provide a good basis for understanding this issue in order
to define a clear scientific attitude as a recommendation.
Key words: genetically modified trees, forest resources, legislation, public attitudes
107
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ALELNA VARIJABILNOST MIKROSATELITA U POPULACIJAMA
DIVLJIH SVINJA U LOVI[TIMA VOJVODINE
Nevena Veli~kovi}, Mihajla Djan, Dragana Obreht i Ljiljana Vapa
Prirodno-matemati~ki fakultet, Novi Sad
Alelna varijabilnost mikrosatelita u populacijama divljih svinja u lovi{tima Vojvodine
analizirana je u ovom radu. U toku lovnih sezona prikupljeni su uzorci mi{i}nog tkiva
divljih svinja, i podeljeni u osam grupa na osnovu lovi{ta u kojima su izlovljene,
tretirane kao “populacije”. Uzorak su ~inile jedinke oba pola i razli~itih starosnih
grupa. Istra`ivanje je pokazalo da su mikrosateliti SW251 i SW2429 koji su vezani sa
QTL-om za dnevni prirast svinje (ADG) primenljivi u analizama geneti~ke
varijabilnosti divljih svinja, pokazuju}i adekvatan nivo polimorfnosti. Na|eno je
ukupno 19 razli~ith alela, 4 u lokusu SW251 i 15 u lokusu SW2429, sa prosekom 9,5
alela po lokusu. Prose~na vrednost uo~ene heterozigotnosti iznosila je 0,67. Uo~eno
je odstupanje od Hardi-Vajnbergove ravnote`e usled statiti~ki zna~ajnog smanjenja
heterozigotnosti u lokusu SW2429. Koeficijent inbridinga Fis za oba lokusa i sve
populacije iznosio je 0,0856, a efektivan broj migranata izra~unat na osnovu
jedinstvenih alela 1,66. Nisu utvr|ene pozitivne korelacije izme|u telesne mase
ispitivanih jedinki i alela koji su na|eni u analiziranim mikrosatelitskim lokusima. U
narednim istra`ivanjima potrebno je uraditi dodatne analize kako bi procenili koje
alele treba favorizovati u procesu selekcije i uzgoja divljih svinja.
Klju~ne re~i: divlja svinja, mikrosateliti, geneti~ka varijabilnost, QTL
MICROSATELLITE ALELIC VARIABILITY OF WILD BOAR
POPULATIONS FROM HUNTING AREAS IN VOJVODINA
The aim of this research was to assess the allelic variability in wild boar populations
from hunting areas in Vojvodina using microsatellites. Muscle tissue samples were
collected, and individuals were devided into eight groups according to hunting areas,
subsequently termed “populations”. The sample included individuals of both sexes
and dif fer ent age classes. The research showed that microsatellites SW251 and
SW2429 which are associated with QTL for average daily gain (ADG) are applicable
in the genetic variability detection in wild boars, showing adequate level of polymorphism. A total number of 19 alleles were found, 4 in locus SW251 and 15 in locus
SW2429, with an average of 9.5. Average observed heterozygosity (Ho) value for
populations was 0.67. Deviation from Hardy-Weinberg equilibrium was found due to
significant heterozygote deficiency detected for locus SW2429. Fis over both loci and
all populations was 0.0856, and the effective number of migrants based on private alleles was 1.66. There was no positive correlation between body weight of analysed individuals and detected alleles of microsatellites loci. Additional analyses are necessary in further research in order to assess which alleles should be favored in the selection and breeding of wild boars.
Key words: wild boar, microsatellites, genetic variability, QTL
108
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PRIMENA GENOTIPSKE KARAKTERIZACIJE
BILJAKA U OPLEMENJIVANJU I SEMENARSTVU
Ankica Kondi}-[pika, Borislav Kobiljski, Ljiljana Brbakli},
Dragana Trkulja i Sanja Treski}
Institut za ratarstvo i povrtarstvo, Novi Sad
Genotipska karakterizacija ili DNK fingerprinting podrazumeva kori{}enje razli~itih
tehnika radi utvr|ivanja specifi~nog DNK profila za odre|eni organizam. Sli~nost
DNK profila razli~itih individua zavisi od stepena njihove genetske bliskosti. Iako je u
po~etku najvi{e kori{}en u medicini i humanoj genetici, koncept DNK fingerprintinga
sve vi{e se primenjuje poslednjih decenija i kod biljaka, `ivotinja i mikroorganizama u
identifikaciji genotipova, populacionoj genetici, sistematici i filogeografiji. DNK
fingerprintingom mogu se utvrditi razlike izme|u biljaka iz razli~itih familija, rodova,
vrsta, sorti, pa ~ak i sestrinskih linija. Brojni su primeri kori{}enja ove tehnike u
razli~ite svrhe, kao {to su: identifikacija sorti, o~uvanje biodiverziteta i upravljanje
genetskim resursima, karakterizacija germplazme, kao i za{tita prava intelektualne
svojine. U ovom radu bi}e re~i o principima i metodama DNK fingerprintinga kod
biljaka, kao i o mogu}nostima njegove primene u oplemenjivanju i semenarstvu u
Srbiji.
Klju~ne re~i: DNK fingerprinting, DNK profil, identifikacija genotipa, primena
APPLICATON OF GENOTYPIC CHARACTERIZATION
OF PLANTS IN BREEDING AND SEED PRODUCTION
Genotypic characterization or DNA fingerprinting involves the use of different techniques to identify specific DNA profile for a particular organism. The similarity of
DNA profiles of different individuals depends on the degree of their genetic similarity. Although initially the most widely used in medicine and human genetics, the concept of DNA fingerprinting is increasingly applied in recent decades in plants, animals and microorganisms in the identification of genotypes, population genetics, systematic and phylogeography. DNA fingerprinting can identify the differences between plants from different families, genera, species, varieties, and even the sister
lines. There are numerous examples of using these techniques for different purposes,
such as cultivar identification, biodiversity conservation and management of genetic
resources, germplasm characterization, and the protection of intellectual property
rights. This paper discusses the principles and methods of DNA fingerprinting in
plants, as well as possibility of its application in plant breeding and seed production.
Key words: DNA fingerprinting, DNA profile, genotype identification, application
109
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
SELEKCIJA POMO]U MOLEKULARNIH MARKERA U STVARANJU
KUKURUZA VISOKOG KVALITETA PROTEINA
Marija Kostadinovi}, Danijela Risti}, Dragana Ignjatovi}-Mici},
Goran Stankovi} i Sne`ana Mladenovi} Drini}
Institut za kukuruz „Zemun Polje”, Beograd
Kukuruz spada me|u najzna~ajnije `itarice u svetu. Proteini kukuruza su siroma{ni u
sadr`aju dve esencijalne aminokiseline - lizina i triptofana. Pobolj{ana hranljiva vrednost opaque2 (o2) mutanta kukuruza otkrivena je 1960-ih godina. Me|utim, o2
mutacija ~ini endosperm kukuruza mekim i bra{njavim, a seme osetljivim na trule`
klipa i {teto~ine skladi{tenog zrna. Kukuruz visokog kvaliteta proteina (VKP) sadr`i
inkorporisan o2 gen i gene modifikatore za tvrdo}u endosperma i sadr`aj
aminokiselina. Selekcija pomo}u molekularnih markera pove}ava pouzdanost i
efikasnost, kao i u{tedu vremena i novca potrebnih za stvaranje VKP hibrida
kukuruza. Upotreba molekularnih markera omogu}ava da se razlikuju homo- i
heterozigotne biljke tokom procesa selekcije. U ovom radu, 400 BC1F1 biljaka iz
ukr{tanja izme|u jedne VKP i dve normalne inbred linije analizirano je pomo}u
specifi~nih SSR markera phi057 i umc1066 koji se nalaze unutar o2 gena. Rezultati su
pokazali da su oba markera uspe{no razlikovala homozigotne i heterozigotne jedinke
i da se mogu koristiti tokom selekcije pomo}u molekularnih markera za prevo|enje
linija standardnog kukuruza u VKP linije.
Klju~ne re~i: kukuruz, opaque2 (o2), kukuruz visokog kvaliteta proteina (VKP),
selekcija pomo}u molekularnih markera
MARKER ASSISTED SELECTION FOR QUALITY PROTEIN MAIZE
Maize is a major cereal crop, worldwide. Maize proteins are deficient in two essential
amino acids - tryptophan and lysine. The enhanced nutritional quality of the maize
mutant opaque2 (o2) was discovered in the 1960s. However, the o2 gene confers an undesirable phenotype of soft, chalky kernels that renders seeds susceptible to storage
pest and ear rots. Quality protein maize (QPM) is a genotype in which o2 gene has
been incorporated along with associated modifiers. Marker assisted selection (MAS)
increases reliability and efficiency, and reduces time and costs taken to obtain a true
QPM. Use of molecular markers allows breeders to determine individual plants containing one or two copies of the mutant o2 alleles. In this study, 400 BC1F1 plants of
the cross between one QPM and two normal inbred lines were analyzed for allelic
variations at the phi057 and umc1066 sites within the o2 gene. Our results showed that
both markers were able to distinguish between homozygous and heterozygous individ u als and were thus good for use in marker as sisted breed ing dur ing the
introgression of QPM into normal maize line.
Key words: maize, opaque2 (o2), quality protein maize (QPM), marker assisted selection (MAS)
110
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
[email protected] ^UVANJA (SHELF LIFE) PLODOVA PARADAJZA
(Licopersicon esculentum MILL.) MUTANATA (NOR I RIN) I NJIHOVIH
HIBRIDA U POSTHARVEST PERIODU
Dejan Cviki}, Jasmina Zdravkovi}, Milan Zdravkovi},
Nenad Pavlovi}, Sla|an Ad`i} i Mladen \or|evi}
Institut za povrtarstvo, Smederevska Palanka
Izvr{ena su ukr{tanja dve linije paradajza sa normalnim periodom sazrevanja plodova
(NR-1 i NR-2) i dve mutantne linije (NR-10 nor i NR-12 rin) kod kojih je period
sazrevanja odlo`en (shelf life). Izvr{ena je determinacija mutantnih gena pomo}u c2
testa na uzorku od 100 plodova F2 generacije.
Plodovi su ubrani 65 dana od antezisa i ~uvani u periodu od 60 dana, kada je izvr{eno
{est evaluacija. Podaci su uzimani u intervalima na svakih 10 dana na roditeljskim
linijama i potomstvu F1 i F2 generacije. Testiranje varijanse izvr{eno je na osnovu
jednofaktorijalne i dvofaktorijalne analize na osnovu kojih su izvr{ena grupna
pore|enja kori{}enjem slo`enih kontrasta. Plodovi su ~uvani u kontrolisanim
uslovima (u mraku, na temperaturi od 5oC).
Mo`emo re}i da genotipovi paradajza sa nor ili rin genom poseduju po`eljne osobine
(odlo`eno sazrevanje, mogu}nost dugog ~uvanja, kao i veliku ~vrstinu ploda) koje
zahtevaju savremeni pravci selekcije, te ih s toga treba uklju~iti u selekcione
programe koje za cilj imaju stvaranje komercijalnih F1 hibrida.
Klju~ne re~i: paradajz, mutant, nor, rin, hibrid
POSTHARVEST SHELF LIFE OF TOMATO (Licopersicon esculentum MILL.)
MUTANATS (NOR AND RIN) AND THEIR HYBRIDS
Two tomato lines with normal maturation (NR-1 and NR-2) have been crossed with
two mutant lines (NR-10 nor and NR-12 rin) with delayed maturation (shelf life). Determination of mutant genes has been done by c2 test on 100 fruits from F2 generation.
Fruits have been picked 65 days from antesis and kept for 60 days, when six evaluations have been done. Data have been collected every 10 days on parental lines and
progeny F1 and F2 generation. Variance testing has been done on the basis of oneand two-factorial analysis and groups compared by contrasts. Fruits have been preserved in controlled conditions (in dark at 5oC).
Tomato genotypes with nor or rin gene have desirable traits (delayed ripening, long
shelf life and firm fruits) for current selection, so they should be included in programmes aiming to create commercial F1 hybrids.
Key words: tomato, mutant, nor, rin, hybrid
111
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DETEKCIJA QTL-OVA ZA PRINOS I KOMPONENTE
PRINOSA U USLOVIMA STRESA SU[E
Ana Nikoli}, Dragana Ignjatovi}-Mici}, Dejan Dodig i Violeta An|elkovi}
Institut za kukuruz „Zemun Polje”, Beograd
Su{a je jedan od glavnih abioti~kih faktora koji uslovljava zna~ajno smanjenje prinosa
kukuruza. Niska heritabilnost prinosa ote`ava evaluaciju i pobolj{anje ovog svojstva.
Pokazano je da su komponente prinosa korelisane sa prinosom, a njihova
heritabilnost je ve}a i ne smanjuje se u uslovima su{e. Savremene tehnike
molekularne genetike omogu}ile su bolje razumevanje geneti~kih faktora koji
kontroli{u ova svojstva, identifikaciju regiona genoma u kome se nalaze kao i
procenu njihovih efekata. U cilju utvr|ivanja pozicije, broja i efekata lousa za
kvantitativna svojstva (QTL-ove) izvr{ena je fenotipska evaluacija F3 familija
ukr{tanja DTP79xB73 za prinos i komponente prinosa u uslovima su{e. Fenotipske
korelacije izme|u ispitivanih svojstava bile su visoke i zna~ajne u ve}ini slu~ajeva.
Detektovano je ukupno 45 QTL-ova za sve ispitivane osobine. Procenat opisane
fenotipske varijabilnosti kretao se u opsegu od 27.46% do 95.85%. Uo~eno je
grupisanje QTL-ova u klastere na nekim hromozomima za razli~ita svojstva, {to
ukazuje na postojanje vezanih gena ili plejotropizam, tako da ovi klasteri mogu biti
korisni u indirektnoj selekciji kukuruza za tolerantnost na su{u.
Klju~ne re~i: kukuruz, tolerantnost na su{u, prinos, komponente prinosa, QTL-ovi
DETECTION OF QTLS FOR YIELD AND YIELD
COMPONENTS UNDER DROUGHT STRESS CONDITIONS
Drought is one of the most important abiotic factors affecting the productivity of
maize. Low yield heritability under drought conditions makes it one of the most difficult traits to evaluate and improve. Yield components are correlated with yield and
have higher heritability than yield itself. Molecular techniques enabled better understanding of genetic factors controlling these traits, identification of specific genomic
regions that harbor them and their effects estimation. F3 families of DTP79xB73
cross were evaluated in the field trials under drought to map quantitative trait loci
(QTLs) underlying yield and yield components, to identify their position and effects.
Phenotypic correlations calculated using Pearson’s coefficient were high and significant for most of the traits. A total of 45 QTLs were identified. Percent of phenotypic
variability determined for the iden tified QTLs was in the range from 27.46% to
95.85%. Several clusters of QTLs for different traits were detected and this indicates
potential existence of linked genes or pleiotropism, which may be useful for indirect
selection in maize improvement for drought tolerance.
Key words: maize, drought tolerance, yield, yield components, QTLs
112
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
IN VITRO TESTIRANJE OTPORNOSTI SUNCOKRETA
(Helianthus annuus L.) PREMA TRIBENURON-METILU
Aleksandra Dimitrijevi}, Ivana Imerovski, Dragana Miladinovi},
Sini{a Joci}, Goran Malid`a i Vladimir Mikli~
Institut za ratarstvo i povrtarstvo, Novi Sad
Suncokret (Helianthus annuus L.) predstavlja najzna~ajniju uljanu kulturu u Srbiji i
jednu od najzna~ajnijih u svetu. Me|utim, prisustvo korova znatno umanjuje prinos
suncokreta, stoga je neophodna upotreba herbicida. Sulfoniluree predstavljaju grupu
herbicida koje uspe{no suzbijaju krupnosemene {irokolisne korove, te je stvaranje
otpornih genotipova suncokreta prema sulfonilureama od izuzetnog zna~aja u
oplemenjivanju. Sulfoniluree mogu da sadr`e razli~ite aktivne materije, od kojih je
jedna tribenuron-metil. U cilju skra}ivanja procesa identifikacije otpornih genotipova
suncokreta, kreiran je in vitro test koji omogu}uje razlikovanje ne samo osetljivih od
otpornih genotipova, ve} i homozigotno otpornih biljaka od heterozigota. U cilju
optimizacije testa, otporni i neotporni genotipovi suncokreta su ispitani u razli~itim
uslovima pH i pri razli~itim koncentracijama tribenuron-metila i odre|ena je najbolja
kombinacija pH i koncentracije herbicida, koja je omogu}ila najjasnije razlikovanje
homozigotno otpornih genotipova od heterozigota.
Klju~ne re~i: in vitro testiranje, suncokret, tribenuron-metil
IN VITRO TESTING OF SUNFLOWER (Helianthus annuus L.)
RESISTANCE TO TRIBENURON-METHYL
Sunflower (Helianthus annuus L.) is the most important oilcrop in Serbia and one of
the most important in the world. However, weeds significantly lower sunflower yield
and therefore, use of herbicides is essential in sunflower breeding. Sulfonylureas are a
group of herbicides that successfully control broadleaf weeds and so, creation of
sulfonylurea re sistant sun flower geno types is of great im por tance in breeding.
Sulfonylureas may con tain dif fer ent ac tive sub stances, one of which is
tribenuron-methyl. In order to shorten the process of identifying sunflower resistant
genotypes a novel in vitro test has been created. This test discriminates not only between sensitive and resistant genotypes, but also between homozygous and heterozygous resistant plants. In order to optimize the test, resistant and susceptible sunflower
genotypes were tested in different pH conditions and at different concentrations of
tribenuron-methyl. The best combination of pH and concentration of herbicide,
which provided the clearest distinction between homozygous resistant genotypes and
heterozygous, was determined.
Key words: in vitro testing, sunflower, tribenuron-methy
113
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
DETEKCIJA PRISUSTVA GENA PLARG U
SUNCOKRETU POMO]U SSR MARKERA
Ivana Imerovski, Aleksandra Dimitrijevi}, Dragana Miladinovi},
Sini{a Joci}, Bo{ko Dedi} i Vladimir Mikli~
Institut za ratarstvo i povrtarstvo, Novi Sad
Plamenja~a je gljivi~no oboljenje suncokreta koje dovodi do velikih gubitaka u
prinosu. Sa pojavom novih patotipova parazita javlja se potreba za identifikacijom i
uno{enjem novih gena otpornosti. Gen Plarg je iz H. argophyllus une{en u gajeni
suncokret, i pru`a otpornost prema svim poznatim rasama plamenja~e. Cilj ovog rada
bio je ispitati da li se prisustvo gena Plarg mo`e utvrditi SSR markerima. U tu svrhu je
izabrano osam markera sa LG1: ORS 610, ORS 543, ORS 1039, ORS 1128, ORS
1182, ORS 509, ORS 710, ORS 662. Testirano je osam uzoraka: otporan roditelj,
neotporan roditelj, dva uzorka iz F1 generacije, kao i ~etiri nasumi~no izabrana
uzorka iz F2 generacije. Svi markeri identifikovali su po dva alela, koja su se zna~ajno
razlikovala izme|u otpornog i neotpornog roditelja. Kod oba uzorka iz F1, kao i kod
tri uzorak iz F2 generacije, svi prajmeri pokazali su prisustvo alela oba roditelja,
navode}i na pretpostavku da su u pitanju biljke koje su heterozigotne za lokus Plarg.
Kod jednog uzorka iz F2 generacije uo~eno je samo prisustvo alela karakteristi~nog za
otpornog roditelja. Dalja istra`ivanja pokaza}e da li se identifikovani markeri mogu
koristiti za marker asistiranu selekciju.
Klju~ne re~i: plamenja~a, suncokret, SSR markeri, Plarg
DETECTION OF PLARG GENE IN SUNFLOWER WITH SSR MARKERS
Downy mildew is a fungal disease that significantly decreases sunflower yield. New
pathotypes of parasite are constantly emerging, and hence comes the need to identify
and introduce new resistance genes into sunflower lines. Gen Plarg originates from H.
argophyllus, and provides resistance to all known downy mildew races. The aim of this
research was to determine if SSR markers could be used to detect the presence of
Plarg gene in sunflower. Eight markers located on LG1 have been chosen: ORS 610,
ORS 543, ORS 1039, ORS 1128, ORS 1182, ORS 509, ORS 710, ORS 662. Resistant
parent, susceptible parent, two random samples from F1 generation, and four random
samples from F2 generation have been tested. All markers identified two alleles that
significantly differed between susceptible and resistant parent. In both tested samples
from F1 and three samples from F2 generation bands characteristic for resistant and
susceptible parent were detected, leading to the conclusion that these samples are
heterozygote for Plarg, whereas one sample from F2 presented as resistant. Further investigation will show if the identified markers are located in close vicinity Plarg gene,
and can be used for MAS.
114
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KA SLI^NOST DVE POPULACIJE
A. retroflexus NA OSNOVU RAPD MARKERA
Milena Simi}, Marija Kostadinovi}, Danijela Risti} i Sne`ana Mladenovi} Drini}
Institut za kukuruz „Zemun Polje”, Beograd
Cilj ovog rada je bio utvr|ivanje geneti~ke sli~nosti, analizom RAPD markera, dve
populacije {tira, A. retroflexus, populacije sa zelenim stablom sa crvenim priimesama
pokrivenim retkim dlakama i populacije sa izrazito maljavim zelenim stablom. Od 40
analiziranih RAPD markera, 31 je dao jasne produkte amplifikacije. Dobijeno je 171
fragmenata, u opsegu od 250 do 3000 bp od kojih 105 fragmenata (61,4%) su bili
polimorfni. Ukupan broj polimorfnih lokusa se razlikovao izme|u markera. GEN
2-80-4 je dao najve}i broj fragmenata (13), a najmanji (2) je dobijen sa prajmerima
OPB05, OPB09, GEN1-70-3. Prose~an broj polimorfnih fragmenata po prajmeru je
5,5. Odre|eni fragmenti su zajedni~ki za obe populacije dok su neki fragmenti
prisutni u ejndoj odsutni u drugoj populaciji. Na osnovu dobijenih rezultata mo`e se
zaklju~iti da su RAPD markeri pogodni za identifikaciju genotipova sa atipi~nom
morfologijom.
GENETIC SIMILARITY OF TWO A. retroflexus
POPULATIONS BASED ON RAPD MARKERS
The aim of this study was to evaluate the level of genetic similarity between two populations of A. retroflexus, population with green plant with pronounced red pigment
admixture and sparse stem hairs and population with green plant and pronounced
stem hairness by RAPD markers. Of 40 primers tested, an initial screening resulted in
selection of 31 decamer primers that produced clear and reproducible RAPD profiles. RAPD primers yielded a total of 171 fragments, ranging from 250 to 3000 bp in
size of which 105 fragments (61,4%) were polymorphic. The total number of polymorphic loci detected varied between primers. The primer GEN 2-80-4 gave the highest number of fragments (13), while the minimum number of fragments in two analysed populations was obtained (2) with OPB05, OPB09, GEN1-70-3 primers. The average number of polymorphic fragments per primer among the two populations was
5,5. Certain amplified bands appeared to be common to both populations, whereas
others were present in one populations, but absent in other. RAPD markers could be
useful for verifying the identity of genotypes with ambiguous or atypical morphology.
115
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KARAKTERIZACIJA Fusarium graminearum KAO PATOGENA SIRKA
U SRBIJI U CILJU UKLJU^IVANJA U SELEKCIJU NA OTPORNOST
Danijela Risti}1, Ivana Stankovi}1, Ana Vu~urovi}1,
Jano{ Berenji2, Branka Krsti}1 i Aleksandra Bulaji}1
1Poljoprivredni fakultet, Zemun
2Institut za ratarstvo i povrtarstvo, Novi
Sad
Ovaj rad je rezultat projekata TR-31073 i III-43001, koje finansira Ministarstvo prosvete i nauke Republike Srbije.
Selekcija na otpornost je najefikasniji i ekolo{ki najprihvatljiviji na~in kontrole
biljnih bolesti. Sve ~e{}e prisustvo vrste Fusarium graminearum Schwabe,
prouzrokova~a plesnivosti klasova strnih `ita i kukuruza, ukazuje na njen zna~aj sa
stanovi{ta smanjenja prinosa gajenog sirka (Sorghum bicolor L. Moench). Prou~avanja
fuzariozne trule`i sirka obavljena su na lokalitetima Ba~ki Petrovac i ^antavir, tokom
2009. i 2010. godine. Vi{e izolata iz obolelih biljaka i semena, ispoljilo je ujedna~enu
patogenost izazivaju}i formiranje crvenkastosme|ih pega u osnovi stabla sirka.
Protokol za identifikaciju uklju~io je lan~anu reakciju polimeraze (PCR), ekstrakciju
ukupne DNA iz micelije (izolat 535-10) i peritecija (izolat 14-11), primenom DNeasy
Plant Mini Kit i prajmera ef1/ef2 (amplifikacija kodiraju}eg gena TEF1-alfa). BLAST
analiza sekvence izolata 535-10 (JF747146) pokazala je 98% do 99% nukleotidne
identi~nosti sa 63 izolata F. graminearum u NCBI bazi podataka. Primenjeni protokol
omogu}ava pravovremenu i preciznu identifikaciju F. graminearum kao preduslov da
se u proces testiranja i selekcije otpornih genotipova sirka uklju~i ova vrsta kao
prevalentna u kompleksu Fusarium vrsta.
Klju~ne re~i: sirak, Fusarium graminearum, identifikacija, selekcija na otpornost
CHARACTERIZATION OF Fusarium graminearum IN SERBIA
WITH THE AIM OF ITS INCLUSION IN THE SORGHUM SELECTION
FOR RESISTANCE
Selection for resistance is the most efficient and ecologically acceptable way of control ling plant dis eases. The more frequent presence of Fusarium graminearum
Schwabe, as a causal agent of head blight of small grain, is a sign of its significance in
sorghum (Sorghum bicolor L. Moench) yield reduction. During 2009 and 2010, the
study of sorghum fusarium rot was conducted on the localities of Ba~ki Petrovac and
^antavir. Several isolates, originating from diseased plants and seed, exhibited uniform pathogenicity causing reddish-necrotic spots on the basal area of the stalk. Identification protocol included polymerase chain reaction (PCR), with total DNA extraction from mycelium (isolate 535-10) and from perithecia (isolate 14-11) using DNeasy
Plant Mini Kit and primers ef1/ef2 (amplifying the TEF1-alfa coding gene). BLAST
analysis of the isolate 535-10 (JF747146) showed 98 to 99% nucleotide identity with
63 F. graminearum isolates deposited in the NCBI GenBank. The implemented protocol provides a timely and precise identification of F. graminearum as prevalent in
Fusarium complex, which enables its inclusion in the process of screening and sorghum selection for resistance.
Key word: sorghum, Fusarium graminearum, identification, selection for resistance
116
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KARAKTERIZACIJA REZERVNIH PROTEINA SEMENA LEGUMINOZA
PRIMENOM MIKRO^IP (LOAC) I SDS PAGE ELEKTROFOREZE
Zorica Nikoli}1, Vuk \or|evi}1, Aleksandra Torbica2 i Aleksandar Miki}1
1Institut za ratarstvo i povrtarstvo, Novi Sad
2Institut za tehnologiju hrane, Novi Sad
Familija leguminoza (Fabaceae, sin. Leguminosae) predstavlja va`an izvor proteina
koji se mogu koristiti u proizvodnji hrane. Cilj rada je bio analiza rezervnih proteina
semena zrnenih leguminoza primenom mikro~ip metode (LoaC) i SDS-PAGE elektro foreze. Relativna ta~nost i preciznost metoda u proceni molekulskih masa
proteina, odre|enih za referentne proteine, ukazuju na sli~ne mogu}nosti ove dve
metode. Ve}a rezolucija i kratko vreme analize daju veliku prednosti LoaC metodi.
Vrlo sli~ni rezultati (R 2 = 0,99) dobijeni su i u odre|ivanju molekulskih masa
rezervnih proteina leguminoza. Tako|e, relativna kvantifikacija proteina pokazuje
dobro slaganje ove dve metode (R2 = 0,81). Obe metode se mogu koristiti za analizu
proteina semena leguminoza, ali je LoaC pogodniji za razdvajanje i karakterisanje
proteina zbog niske potro{nje reagenasa, jednostavnog rukovanja i brzog dobijanja
rezultata. Otuda, u oplemenjivanju leguminoza, kada je potrebno analizirati veliki
broj uzoraka, prednost ima LoaC metoda.
Klju~ne re~i: zrnene leguminoze, rezervni proteini semena, Lab-on-the-Chip
LEGUMES SEED STORAGE PROTEINS CHARACTERIZATION
BY SDS PAGE AND LAB-ON-A-CHIP ELECTROPHORESIS
In the production of food, the legume family (Fabaceae, syn. Leguminosae) contributes
with some of the most important protein-rich seeds. The study was conducted to analyze the seed storage proteins of grain legumes cultivars by lab-on-a-chip (LoaC) and
SDS-PAGE electrophoresis. Using referent proteins, relative accuracy and precision
in molecular weight estimation indicate a similar power of these two methods and the
performance of LoaC system in terms of protein sizing is in the range of conventional
slab gels, while higher resolution and analysis time give great advantages to it. Molecular weight estimation of grain legume storage proteins gave very similar results (R2 =
0.99). Relative quantification of separated proteins indicate good agreement of two
techniques (R2 = 0.81). Both methods can be used to analyze legume seed proteins
composition, but LoaC is actually more suited for separation and detection of proteins, enabling low reagent consumption, easy operation and faster turnaround and it
is suitable for legume quality screening and cultivar development where large numbers of samples need to be analyzed.
Key words: grain legumes, seed storage proteins, Lab-on-the-Chip
117
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POLIMORFNOST DNK SEKVENCI ZA HOMOLOGE GRAS-SCL,
TF GENA KOJI KODIRA FACTOR TRANSKRIPCIJE ZA TOLERANTNOST
TOPOLA NA ZASLANJENOST
Vladislava Galovi}1, Matthias Fladung2, Cristina Vettori3 i Sa{a Orlovi}1
3Plant
1Institut za nizijsko {umarstvo i `ivotnu sredinu, Novi Sad
2Johann-Heinrich-von-Thuenen Institute (vTI), Grosshansdorf, Germany
Genetics Institute, Division of Florence Polo Scientifico CNR, Sesto Fiorentino (FI), Italy
Istra`ivanja odgovora biljaka na stres u oblasti funkcionalne genomike, posebno
identifikacija osnovnog seta transkripata koji su povezani sa stresom, su klju~ni i za
eksploataciju tolerantne germplazme kao i za razvoj tolerantnih useva kroz genetske
manipulacije. Najnovije publikacije u vezi sa indukcijom GRAS protein SCL7 pri
stresu su{e i zaslanjenosti kod topola su pokazale da je ovaj gen potencijalno koristan
za primenu u geneti~kom in`enjerstvu pri unapredjenju tolerancije drve}a na stres
su{e i zaslanjenosti, te je GRAS / SCL transkripcioni faktor (TF) izabran kao
kandidat gen za istra`ivanja. Za ovu studiju su odbrana 4 razli~ita agronomski va`na
doma}a klona topole (tri pripadaju P. deltoides i jedan P. euramericana). Polimorfizam
DNK sekvenci je opisan izme|u svih vrsta i na svim uzorcima putem polimorfnih
obrazaca i determinisan je broj polimorfnih mesta. Pri pore|enju ~etiri Populus vrste
ustanovljeni su polimorfni obrasci izme|u vrsta koji su dobijeni na osnovu broja
supstitucija fiksnih u obe vrste, polimorfni za jednu ali ne i druge vrste i polimorfni u
obe vrste. Autor upu}uje zahvalnost COST akciji FP0905 za finansiranje ovoga rada
putem u~e{}a na kratkoro~noj nau~noj misiji (STSM).
Klju~ne re~i: Populus sp., abioti~ki stres, kandidat geni, SNPs
POLYMORPHISMS OF DNA SEQUENCES OF HOMOLOGUES
GRAS/SCL TF, A GENE ENCODING A TRANSCRIPTION FACTOR
WITH RELEVANCE TO SALINE TOLERANCE
Functional genomics studies of plant stress responses, particularly the identification
of a core set of stress-related transcripts, are crucial for both tolerant germplasm exploitation and tolerant crop development through genetic manipulation. Recent publications related to salt and drought-inducible poplar GRAS protein SCL7 showed
that this gene is potentially useful for engineering drought and salt tolerance in trees,
thus focus our attention to a GRAS/SCL transcription factor (TF) as a candidate gene
for the research work. For this study 4 different clones agronomicaly important for
Serbia (three belonging to P. deltoides and one of P. euramericana) were sampled.
DNA sequence polymorphisms were described within species and across all specimens by their polymorphism patterns and the number of polymorphic sites were revealed. In pairwise compar i sons of the four Populus spe cies, pat terns of
polymorphisms between species were obtained by the number of substitutions fixed
in both species, polymorphic in one but not the other species and polymorphic in both
species. The author thank the COST Action FP0905 for its contribution to this work
through financing the Short Term Scientific Mission.
Key words: Populus sp., abiotic stress, candidate genes, SNPs
118
Sekcija / Section 7
Biodiverzitet i geneti~ki resursi
Biodiversity and genetic resources
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
EROZIJA GENETI^KOG DIVERZITETA STRNIH @ITA
Sofija Petrovi} i Miodrag Dimitrijevi}
Poljoprivredni fakultet, Departman za ratarstvo i povrtarstvo, Novi Sad
@itarice imaju va`nu ulogu u ljudskoj ishrani, pa se oplemenjivanjem te`i da se dobiju
sorte sa visokim geneti~kim potencijalom za prinos. Savremena poljoprivredna
proizvodnja uklju~uje {irenje intenzivnih sorti na velikim povr{inama, {to dovodi do
su`avanja kriterijuma oplemenjivanja. Posledica je drasti~no smanjenje broja vrsta i
genotipova (geneti~ka erozija), odnosno su`enje biolo{ke raznovrsnosti lokalnih
populacija i spontanih srodnika (biodiverziteta) strnih `ita.
Na osnovu detaljnog inventarisanja na teritoriji Crne Gore i delimi~no u isto~noj
Srbiji, uo~ena je ugro`enost genskog fonda strnih `ita. Razlog ovome je prelazak na
drugu vrstu poljoprivredne proizvodnje, promena namene zemlji{ta, prisustvo
stara~kih doma}instava, kao i kori{}enje intenzivnih sorti `itarica. Stare sorte i
lokalne populacije predstavljaju originalnu geneti~ku varijabilnost, mogu da se
koriste u programima oplemenjivanja i organskoj proizvodnji. Stoga je bitno da se
preduzmu mere za o~uvanje geneti~kih resursa `itarica.
GENETIC EROSION OF DIVERSITY IN CEREALS
Cereals play an important role in human nutrition. Consequently, one of the main
goals in breeding is to obtain varieties with high genetic potential for yield. Modern
agricultural production includes the expansion of intensive varieties over large areas
that lead to narrow selection criteria in breeding programs. The consequence is a
drastic reduction in the number of species and genotypes (genetic erosion), or harming biological diversity of local populations, and spontaneous relatives (biodiversity)
in cereals.
Based on detailed inventories of the territory of Montenegro and to some extent in
Eastern Serbia, a endangerment of gene pool in cereals could be denoted. The reason
for this is a transition to another type of agricultural production, changing land purposes, the presence of nursing homes, and use of intensive varieties. Old varieties and
local populations represent the original genetic variability that could be used in
breeding programs and organic production. It is therefore essential to take measures
to preserve the genetic resources of cereals.
120
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PROJEKAT „SA^UVAJMO STARE VOJVO\ANSKE
SORTE POVR]A“ - ^ETIRI GODINE POSLE
Vladimir Sabado{, Gordana Forgi}, Zoran Boca i Olivera Sekuli}
PSS „Sombor“, Sombor
Akcija prikupljanja semena starih sorti povr}a i drugih biljnih vrsta pokrenuta je od
strane PSS „Sombor“ 03.12.2007. godine, i u njoj su u~estvovali u~esnici 22 osnovne
{kole na podru~ju Zapadno ba~kog okruga i prikupljeno je ukupno 5296 uzoraka
povr}a i drugih biljnih vrsta i aktivno je u~estvovalo 617 u~enika. Nakon prikupljanja
uzoraka semena u periodu od 2007 do 2011 godine, uzorci semena su determinisani,
napravljena je baza podataka, izvr{ena setva, izvr{eno ocenjivanje i opis biolo{kih i
proizvodnih svojstava tokom ove ~etiri godine. Tako|e je uspostavljena saradnja sa
nau~no istra`iva~kim institucijama u zemlji i inostranstvu, za nastavak istra`ivanja
prikupljenog materijala ovim projektom. Najve}e interesovanje izazvali su uzorci
starih sorti i populacija boranije, pasulja, paprike, paradajza, bundeve, dinje, tikve,
maka, cve}a i za~inskog bilja.
Klju~ne re~i: uzorak, seme, sorta, populacija, povr}e
PROJECT „SAVE OLD VEGETABLE VARIETIES
OF VOJVODINA“ - FOUR YEARS AFTER
PSS Sombor strated with action of collecting seed of old varieties of vegetables and
other plant species in 03.12.2007., pupils from 22 primary schools from West Ba~ka
district collected 5296 samples of vegetables and other plant species, activly participated 617 pupils. After seed was collected in period from 2007 to 2011 year seed samples were determinated , information base was established, we saw samples and describe and evaluate biological and production characteristics during this four year period. Also cooperation was established with scientific and research institutions in Serbia and with foreign countries. For prolongation of research on collected matherial in
this project most interest was about samples of old varieties and populations of french
beans, beans, pepper, tomato, pumpkin, melon, poppy, courgette, flowers and spice
herbs.
Key words: sample, seed, variety, population, vegetables
121
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
BIODIVERZITET DIVLJIH VO]NIH VRSTA U SRBIJI
Vladislav Ognjanov, Mirjana Ljubojevi}, Du{ica Bo{njakovi},
Goran Bara}, Emina Mladenovi} i Marija Predojevi}
Poljoprivredni fakultet, Departman za vo}arstvo, vinogradarstvo,
hortikulturu i pejza`nu arhitekturu, Novi Sad
U poslednje tri godine u~injeni su brojni napori da se kolekcioni{e, sa~uva, opi{e i
oceni germplazma vo}nih vrsta Balkanskog poluostrva. Zahvaljuju}i jedinstvenom
biodiver zi tetu Balkansko poluostrvo se mo`e smatrati sekundarnim centrom
geneti~kog diverziteta nekoliko vrsta: Malus x domestica, M. sylvestris, P. communis, P.
cerasifera, P. persica, P. mahaleb, P. armeniaca, P. amygdalus, P. nana, J. regia, C.
colurna, C. avellana, C. sativa, F. vesca, O. europaea, C. mas, S. nigra, Sorbus sp, Rosa sp,
Morus sp.
Nekoliko sakuplja~kih ekspedicija je potvrdilo da su divlje vo}ne vrste vredan
prirodan resurs. One predstavljaju nepresu{ni genofond veoma va`nih vrsta koje daju
plod izuzetnog kvaliteta i visoke nutritivne vrednosti, od zna~aja za ljudsku ishranu,
medi cin ske svrhe i industrijsku preradu. Ispitivane su najva`nije morfolo{ke,
ekolo{ke i biolo{ke karakteristike vrsta P. domestica, C. mas, S. nigra, M. alba i Rosa sp.
Cilj je bio razvijanje dugoro~ne strategije u selekciji drvenastih vrsta, njihovo
unapre|enje razli~itim metodama oplemenjivanja, upotreba selekcionisanih
proizvoda i njihovo uklju~ivanje u intenzivnu vo}arsku proizvodnju.
Klju~ne re~i: diverzitet, oplemenjivanje, selekcija, {umske vo}ne vrste
BIODIVERSITY OF WILD FRUIT SPECIES OF SERBIA
Over the past three years, there has been an effort to collect, conserve, protect, characterize and evaluate fruit germplasm on the Balkan Peninsula. Thanks to the unique
diversity, the Balkan Peninsula could be considered as secondary center of genetic diversity for several species such as Malus x domestica, M. sylvestris, P. communis, P. cerasifera, P. persica, P. mahaleb, P. armeniaca, P. amygdalus, P. nana, J. regia, C. colurna, C.
avellana, C. sativa, F. vesca, O. europaea, C. mas, S. nigra, Sorbus sp, Rosa sp, Morus sp.
Several fild collecting trips confirmed that forest fruit species are very important natural resources. It is an inexhaustable genofond of extremely important species that
yield fruit of excellent quality and high nutritive value, which are used in the human
diet, medicinal application and for industrial processing. The most important morphological, ecological, and biological characteristics were studied for P. domestica, C.
mas, S. nigra, M. alba and Rosa sp. The aim was development of long term interactive
strategy for tree selection, improvement using different breeding methods, use of selected products and their integration into intensive fruit growing.
Key words: breeding, diversity, selection, wild fruit species
122
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KI DIVERZITET POPULACIJA LUCERKE
DETEKTOVAN KORI[]ENJEM RAPD MARKERA
Gordana [urlan-Momirovi}1, Ramadan Salem Ahsyee2, Omar al-Sloge3,
Sanja Vasiljevi}4, Irena ]ali}1 i Gordana Brankovi}1
1Poljoprivredni fakultet, Zemun
2Fakultet Prirodnih Nauka, Tripoli, Libija
3Biolo{ki istra`iva~ki centar, Tripoli, Libija
4Institut za ratarstvo i povrtarstvo, Novi Sad
Lucerka (Medicago sativa L.) je va`na krmna leguminoza u Libiji sa zna~ajnom
ulogom u obezbe|ivanju jeftinog krmiva visoke nutritivne vrednosti. Geneti~ki
diverzitet devet Libijskih gajenih populacija lucerke je prou~avan kori{}enjem
trinaest RAPD selektivnih prajmer kombinacija. Broj polimorfnih fragmenata
detektovanih po prajmerskoj kombinaciji je bio u opsegu od 8 do 46 sa prosekom od
24 fragmenta. Broj polimorfnih fragmenata se kretao od 6 (Atalia populacija) do 37
(Gabsia populacija). Dendrogram zasnovan na UPGMA klaster analizi je grupisao
devet populacija u dva glavna klastera. Prvu grupu ~ine slede}e populacije: Fazania,
Atalia, Masratia, Zawia, Denamo Ferade i Arezona. Drugu grupu ~ine Tagoria,
Gabsia i Wade Alrabeh populacije. Najmanja geneti~ka dis tance (0.058)je
ustanovljena za Fazania populaciju dok je najvi{a (0.655) detektovana za Denamo
Ferade. Prose~na geneti~ka distanca je iznosila 0.356. Jednostavnost RAPD markera
kao metode za analizu geneti~kog diverziteta populacija lucerke i detekciju
geneti~kog polimorfizma, je potvr|ena u na{em istra`ivanju, sa rezultatima koji se
mogu primeniti u oplemenjiva~koj praksi.
Klju~ne re~i: Medicago sativa, geneti~ki diverzitet, geneti~ka distanca, RAPD
GENETIC DIVERSITY OF ALFALFA POPULATIONS
REVEALED BY RAPD MARKERS
Alfalfa (Medicago sativa L.) is an important forage legume in Libya with significant
role in providing cheap forage of high nutrition value. Genetic diversity of nine Libyan cultivated populations of alfalfa was studied using thirteen RAPD selective
primer combinations. The number of polymorphic fragments detected per primer
combination ranged from 8 to 46 bands with an average of 24 bands. The number of
polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The dendrogram based on UPGMA cluster analysis grouped the nine populations into the two main clusters. The first group included following populations:
Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group
was composed of Tagoria, Gabsia and Wade Alrabeh alfalfa populations. The lowest
genetic distance (0.058) was observed in Fazania population and the highest (0.655)
was detected in Denamo Ferade. The average genetic distance was (0.356). The simplicity of RAPD assays as a method for the analysis of genetic diversity among alfalfa
populations, and of detection of genetic polymorphisms, is confirmed in our study,
and results can be utilized in breeding practice.
Key word: Medicago sativa, genetic diversity, genetic distance, RAPD
123
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PROIZVODNE I MORFOLO[KE OSOBINE RAZLI^ITIH
DOMA]IH POPULACIJA MAKA (Papaver somniferum L.)
Vladimir Sabado{, Bo`ana Pani} Grba, Jelena Ivan i Sonja Krsmanovi}
PSS „Sombor“, Sombor
Ispitivanje morfolo{kih i proizvodnih osobina lokalnih populacija maka inspirisana je
slede}im ~injenicama: da je mak za prehrambenu industriju u Srbiji zna~ajna sirovina
koja se ve}inom uvozi iz susednih zemalja i zato se izdvajaju zna~ajna devizna
sredstva, da kod nas ne postoji ni jedna priznata sorta na sortnoj listi, a postoji u
okviru PSS „Sombor“ kolekcija populacije semena maka ~ija se razli~itost odlikuje u
slede}im osobinama biljaka maka: visina biljke, boja cveta, oblik i veli~ina ~aure, boja
i krupno}a semena, sadr`aj ulja u semenu maka, du`ina vegetacije, otpornost na niske
temperature kao bolesti tokom vegetacije ove uljane biljke.
Ispitivanja su vr{ena u periodu od 2008. do 2011. godine na oglednom polju i laboratorijama PSS „Sombor“ doo, Sombor.
Klju~ne re~i: mak, ~aura, seme, sadr`aj ulja, ogledno polje
PRODUCTION AND MORPHOLOGICAL AHARACTERISTICS OF
DIFFERENT DOMESTIC POPULATIONS OF POPPY (Papaver somniferum L.)
Testing of morphological and production characteristics of local population of poppy
was inspired by this facts: poppy is very important row material for food industry in
Serbia, we mostly import poppy from naightbour countries and for that reason important amount of foreign currency is spending, we do not have any recognized variety in
official list of varieties in Serbia, but in PSS „Sombor“ we have population collection
of seed with many different characteristics: plant hight, colour of flower, shape and
size of seed case, colour and size of seed, oil content in seed of poppy, duration of vegetation period, cold endurance, disease during vegetation period of this species. testing period was from 2008 to 2011 year in testing trial field and laboratories in PSS
„Sombor“ doo, Sombor.
Key words: poppy, seed case, seed, oil content, trial field
124
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
RAZVOJ NACIONALNE STRATEGIJE I PROGRAMA
O^UVANJA I [email protected] KORI[]ENJA BILJNIH GENETI^KIH
RESURSA ZA HRANU I POLJOPRIVREDU U SRBIJI
Milena Savi} Ivanov, Maja Je~menica i Danica Berlekovi}
Ministarstvo poljoprivrede, trgovine, {umarstva i vodoprivrede,
Direkcija za nacinalne referentne laboratorije, Beograd
Postojanje i primena nacionalne strategije i programa neophodni su preduslovi za rad
na o~uvanju i odr`ivom kori{}enju geneti~kih resursa za hranu i poljoprivredu.
Pored nesumnjivog bogatstva na{e zemlje biodiverzitetom i postojanja brojnih
oplemenjiva~kih instituta sa dugom tradicijom, rad na objedinjavanju ovih resursa bio
je pokrenut 1989. godine projektom „Formiranje banke biljnih gena Jugoslavije“.
Dalji napori koji su ulagani da se oformi banka biljnih gena i stvori nacionalna stragija
i program do sada nisu dali krajnji rezultat.
Zakonom o bezbednosti hrane (Slu`beni glasnik RS broj 41/09) osnovana je Direkcija
za nacionalne referentne laboratorije, kao organ uprave u sastavu Ministarstva poljoprivrede, trgovine, {umarstva i vodoprivrede, a u njenom sastavu se nalazi i Banka
gena.
Formiranje Banke gena i stvaranje nacionalne strategije i programa, odvija se uz
finansijsku i stru~nu pomo} realizacijom dva projekta: „Razvojna mre`a jugoisto~ne
Evrope za biljne geneti~ke resurse“ (SEEDNet) i FAO projekat tehni~ke podr{ke
„Podr{ka razvoju nacionalnog programa za biljne geneti~ke resurse za hranu i
poljoprivredu“ FAO/TCP/YUG/3203(D).
Klju~ne re~i: strategija, program, biodiverzitet, geneti~ki resursi, projekat
NATIONAL STRATEGY AND PROGRAMME FOR THE CONSERVATION
AND SUSTAINABLE USE OF PLANT GENETIC RESOURCES FOR FOOD
AND AGRICULTURE IN SERBIA
The prerequisite for work on the conservation and sustainable use of plant genetic resources for food and agriculture is to have and implement a National strategy and
programme.
With undoubtedly abundant biodiversity and numerous breeding companies with
long tradition, Serbia started gathering all these resources within the project ’Establishment of the Yugoslav plant gene bank’ in 1989. Further efforts put into forming
the Plant Gene Bank and developing the National strategy and programme have not
given the final result so far.
The Directorate for National Reference Laboratories (DNRL) was founded pursuant
to the Food Safety Law (Official Gazette of the Republic of Serbia, No. 41/09) within
the Ministry of Agriculture, Trade, Forestry and Water Management, and the Plant
Gene Bank became one of DNRL’s organisational units.
The process of establishment of the Plant Gene Bank and development of the National strategy and programme has been assisted financially and technically by two
projects: “South East European Development Network on Plant Genetic Resources”
(SEEDNet) and “Support to the development of the national programme for plant
genetic resources for food and agriculture” FAO/TCP/YUG 3203(D).
Key words: strategy, programme, biodiversity, genetic resources, project
125
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ME\UZAVISNOST MORFOLO[KIH OSOBINA
KOD POPULACIJA KUKURUZA (Zea mays L.)
Danijela Risti}, Jelena Van~etovi}, Sne`ana Mladenovi} Drini},
Natalija Kravi} i Dragana Ignjatovi}-Mici}
Institut za kukuruz “Zemun Polje”, Beograd
Za selekciju kukuruza, izvori po`eljnih svojstava koji se nalaze u bankama gena dobijaju sve ve}i zna~aj. Materijal koji se ~uva u banci gena Instituta za kukuruz, po obimu
i sadr`aju, pru`a velike mogu}nosti za potrebe selekcije. Iako je va`no znati o me|usobnoj povezanosti morfolo{kih karakteristika sa prinosom, postoji malo informacija
o njihovim korelacijama kod populacija kukuruza. Glavni cilj ovog istra`ivanja je bilo
utvr|ivanje odnosa izme|u pojedinih morfolo{kih osobina kod odabranih populacija
kukuruza. U poljskim ogledima 2007. i 2008. godine je testirano 54 lokalnih i 6
introdukovanih populacija u ~etiri ponavljanja, po randomiziranom blok dizajnu.
Rezultati pokazuju jaku pozitivnu korelaciju izme|u broja dana do svilanja i visine
biljke, odnosno visine gornjeg klipa, kao i izme|u visine gornjeg klipa i du`ine lista.
Zna~ajna korelacija je uo~ena i izme|u broja listova po biljci i broja primarnih grana
metlice, odnosno vlage u zrnu. Najve}a korelacija je na|ena kako izme|u visine biljke
i visine gornjeg klipa, tako i izme|u visine biljke i du`ine lista. Jaka negativna
korelacija je utvr|ena izme|u broja redova zrna i {irine zrna.
Klju~ne re~i: korelacija, morfolo{ke osobine, populacije, Zea mays L.
ASSOCIATION BETWEEN MORPHOLOGICAL
TRAITS AMONG MAIZE (Zea mays L.) LANDRACES
Maize breeding depends upon genetic sources of desirable traits, maintained within
existing gene bank germplasm. The material from the Maize Research Institute gene
bank offers the great opportunities for breeding, considering its size and content. Although it is important to know the associations between morphological traits and
grain yield, there is limited information about these correlations in maize landraces.
The main intention of this study was to determine relationship between some morphological traits among maize landraces. Fifty four local and six introduced maize
landraces were tested in four-replicate trials set up according to the randomised block
design during 2007 and 2008 season. Collected data revealed strong positive correlation between days to silking and plant and ear height, as well as, between ear height
and leaf length. Number of leaves per plant was significantly correlated with the number of primary branches on tassel and grain moisture. The highest correlations were
found both between plant and ear height and between plant height and leaf length. A
strong negative correlation was found between number of kernel rows and kernel
width.
Key words: correlations, landraces, morphological traits, Zea mays L.
126
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
RAST KORENA I NADZEMNOG DELA KLIJANACA KUKURUZA
(Zea mays L.) TOLERANTNIH I OSETLJIVIH NA SU[U: ODGOVOR NA
STRES IZAZVAN PEG-OM
Natalija Kravi}, Mirjana Vuleti} i Violeta An|elkovi}
Institut za kukuruz „Zemun Polje“, Beograd
Su{a izaziva zna~ajno smanjenje prinosa zrna kukuruza u svetu. Brojna istra`ivanja
potvr|uju ve}u osetljivost korenovog sistema na promene sadr`aja vode u zemlji{tu u
odnosu na nadzemni deo. U laboratorijskim uslovima osmotikumi se koriste kao
simulatori stresa su{e. Polietilen glikol (PEG) 10000 je osmotikum, ~iji molekuli, zbog
velike te`ine, ne prodiru u korenov sistem. Cilj ovog istra`ivanja je ocena du`ine i
sve`e mase korena i nadzemnog dela, kao i sadr`aja prolina kod genotipova sa
razli~itom tolerantno{}u prema su{i u poljskim uslovima. Nakon testiranja 6000
uzoraka iz banke gena u uslovima kontrolisane su{e u Egiptu, kao i u Srbiji i
Makedoniji, izdvojen je 41 genotip. Od ovih genotipova odabrano je 5 inbred linija
koje su izlo`ene uticaju 4% PEG-a u fitotronskoj komori. Rezultati su pokazali
smanjenje porasta korena (1.35-40.1%) i sve`e mase (11.0-61.9%); sadr`aj prolina je
varirao od 22% smanjenja do 188% pove}anja u odnosu na kontrolu. Redukcija
du`ine nadzemnog dela je varirala od 1.87 do 45.98% i sve`e mase od 12.32 do
64.16%; pove}anje sadr`aja prolina se kretalo u opsegu od 0.5 do 152.14% u odnosu
na kontrolu.
Klju~ne re~i: polietilen glikol, prolin, sve`a masa, Zea mays L.
ROOT AND SHOOT GROWTH IN DROUGHT SENSITIVE
AND TOLERANT MAIZE (Zea mays L.) SEEDLINGS: RESPONSE
TO STRESS INDUCED BY PEG
Drought severily decrease maize grain production worldwide. Evidence have shown
that roots are more sensitive to small changes in soil water content than shoot growth.
Under laboratory conditions the osmolites are used to mimic drought stress. Polyethilene glycol (PEG) with a molecular weight of 10000 Da is an osmolyte that does
not enter the root system. The objective of this study was to evaluate root and shoot
growth, fresh weigh and proline content among genotypes with different drought tolerance under field conditions. After testing of 6000 gene bank accessions under controlled drought in Egypt, as well as in Serbia and Macedonia, 41 genotypes were chosen, considered as drought tolerant. Out of them, five inbred lines were exposed to
4% PEG in growth chamber. The root growth reduction vary from 1.35 up to 40.1%;
fresh weight was reduced from 11.0 to 61.9% and proline content was in a range of reduction of 22% to increase of 188% compared to controlled condition. The shoot
growth was reduced from 1.87 up to 45.98%; fresh weight varies from 12.32 to 64.16%
and proline content increased from 0.5 to 152.14 % compared to control.
Key words: fresh weight, polyethilene glycol, proline, Zea mays L.
127
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KLASTER ANALIZA OSOBINA PO DESKRIPTORU
POPULACIJA KUKURUZA KOJE [email protected] CMS
Sofija Bo`inovi}, Nikola Gr~i}, Dragana Ignjatovi}-Mici}, Ana Nikoli},
Violeta An|elkovi}, Milo{ Crevar i Jelena Van~etovi}
Institut za kukuruz „Zemun Polje“, Beograd
Banka gena Instituta za kukuruz sadr`i kolekciju od oko 6000 uzoraka kukuruza. U
okviru ove kolekcije na|eno je preko 100 izvora citoplazmatske mu{ke sterilnosti
(cms). Od 96 ispitanih populacija iz biv{e Jugoslavije, 81 pripada cms-S tipu, 12 cms-C
tipu i 3 cms-T tipu. Genotipovi su na osnovu deskriptora za kukuruz opisani za
osobine metlice, klipa, lista i zrna kukuruza. Cilj je bio da se ispita podudarnost u
klaster analizi zasnovanoj na ispitivanim osobinama i tipu cms-a. Ura|ena je
hijerarhijska klaster analiza po UPGMA metodi za sve ispitivane osobine zajedno,
kao i za osobine metlice, klipa, lista i zrna posebno. Dendrogram dobijen na osnovu
klaster analize za sve osobine izolovao je jedan genotip u cms-C tipu van glavnog
podklastera. Glavni podklaster je podeljen na jedan manji sa 20 genotipova i ve}i sa
preostalim genotipovima. Samo dva podklastera grupisala su genotipove sa istim
tipom cms-a (cms-S). Na osnovu dendrograma za sve ispitivane osobine ne postoji
vidljiva veza izme|u ovih osobina i tipa cms-a. Dendrogrami dobijeni klaster
analizom pojedina~nih grupa osobina tako|e nije pokazao nikakvu vezu izme|u tipa
cms-a i analiziranih osobina.
CLUSTER ANALYSIS FOR DESCRIPTOR TRAITS
OF MAIZE LANDRACES CONTAINING CMS
Maize Research Institute gene bank maintains a collection of around 6000 maize accessions. Within this collection over 100 sources of cytoplasmic male sterility (cms)
were found. Out of 96 examined ex-Yugoslav landraces, 81 are of cms-S, 12 of cms-C
and 3 of cms-T type. These genotypes were described following maize descriptor for
tassel, ear, leaf and grain traits. The aim of the study was to determine a possible congruence between clustering based on traits evaluation and type of cms. Hierarchical
cluster analysis was conducted by UPGMA method for all the tested traits together
and also for the tassel, ear, leaf and grain traits separately. Dendrogram obtained by
cluster analysis of all traits has isolated one genotype in cms-C type, outside the main
subcluster. The main subcluster is divided into one smaller branch with 20 genotypes,
and a bigger one with the remaining genotypes. Only two subclusters grouped genotypes with the same type of cms (S type). Based on the dendrogram for all traits, there
is no congruence with cms type. Dendrograms obtained by cluster analysis of individual groups of traits also didn’t show any congruence between the cms type and the analyzed traits.
128
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KLASIFIKACIJA LOKALNIH POPULACIJA KUKURUZA
TOLERANTNIH NA SU[U U DIVERGENTNE GRUPE
Vojka Babi}1, Jelena Van~etovi}1, Slaven Prodanovi}2,
Milosav Babi}1 i Violeta Andjelkovi}1
1Institut za kukuruz „Zemun Polje“, Beograd
2Poljoprivredni fakultet, Zemun
U nekim slu~ajevima core kolekcije formiraju se sa ciljem da predstavljaju samo deo
kolekcije sa specifi~nim svojstvima, ili core kolekcije na odre|enu osobinu. Procedura
za formiranje core kolekcije sastoji se od vi{e koraka, a svaki od njih mo`e da bude
manje ili vi{e slo`en u zavisnosti od raspolo`ivih informacija i kori{}ene procedure.
Na osnovu prethodnih istra`ivanja u poljskim uslovima u Egiptu, koja su uklju~ivala
celokupnu banku gena Instituta za kukuruz Zemun Polje (6371 uzorak), pored
ostalih, odabrana je 321 lokalna populacija, kao mogu}i izvori tolerantnosti na su{u.
Kako su lokalne populacije nastale u dugom nizu godina prirodnog ukr{tanja i
odabiranja u na{im agroekolo{kim uslovima, njima je posve}ena posebna pa`nja. Na
osnovu morfolo{ke analize po IPBGRI deskriptoru lokalne populacije su podeljene u
divergentne grupe. Kori{}ene su stepwise i hirerarhijske procedure: prvo ~ine}i veliku
podelu, a onda dele}i te grupe na manje podgrupe. Na taj na~in lokalne populacije su
podeljene u 17 divergentnih grupa. Slede}i korak }e se odnositi na odluku o broju
core populacija koje }e se formirati (sa aspekta kori{}enja u procesu oplemenjivanja)
kao i o broju i konkretnom odabiru lokalnih populacija koje }e u~estvovati u
formiranju.
Klju~ne re~i: core kolekcija, klasifikacija, lokalne populacije kukuruza
THE CLASSIFICATION OF LOCAL MAIZE POPULATIONS
TOLERANT TO DROUGHT INTO DIVERGENT GROUPS
The core collections sometimes formed with the aim to represent just a part of the collection with specific traits, or core collections for a certain trait. The procedure for the
core collection formation consists of several steps, and each step can be more or less
complex in dependence on available information and the applied procedure.
Based on previous studies that included the entire gene bank of the Maize Research
Institute, Zemun Polje (6371 accessions) carried out under field conditions in Egypt,
321 local populations were selected as possible sources of tolerance to drought. Special attention was paid to local populations because they were developed in the course
of many years of natural crosses and selection under agroecological conditions of our
country. According to morphological traits recorded using the IPBGRI descriptor,
the local populations were classified into divergent groups. Stepwise and hierarchical
procedures were applied: firstly, a great division was made, and then these groups
were divided into smaller sub-groups. In such a way, local populations were classified
into 17 divergent groups. The next step will be related to the decision on the number
of core populations that will be formed (from the aspect of the utilisation in the process of breeding), as well as, on the number and actual selection of local populations
which will be included into the formation.
129
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PROU^AVANJE NEKIH POPULACIJA PASULJA
(Phaseolus vulgaris L). POREKLOM IZ CENTRALNE SRBIJE
Milan Zdravkovi}1, Jasmina Zdravkovi}1, Rado{ Pavlovi}2, Zdenka Girek1,
Nenad Pavlovi}1, Dejan Cviki}1 i Suzana Pavlovi}1
1Institut
za povrtarstvo, Smederevska Palanka
faklultet, ^a~ak
2Agronomski
Pou~avanje populacija pasulja prikupljenih tokom rada na terenu, poseta
proizvo|a~ima ili na lokalnim pijacama centralne Srbije, ima izuzetno veliki zna~aj za
program oplemenjivanja pasulja u Institutu za povrtarstvo d.o.o. u Smederevskoj
Palanci. U po~etnim istra`ivanjima obuhva}ene su standardne osobine: visina stabla,
broj mahuna po biljci, broj zrna u mahuni, masa 1000 semena, oblik i boja zrna,
potencijalna rezistentnost. Istra`ivanjima su obuhva}ene 37 populacija.
Od posmatranih osobina u radu su prokazane tri osobine: visina stabla, broj zrna u
mahuni i masa 1000 semena
Klju~ne re~i: pasulj, visina stabla, broj zrna u mahuni, apsolutna masa zrna
STUDY OF SOME BEAN (Phaseolus vulgaris L) POPULATIONS
ORGINATING FROM CENTRAL SERBIA
Study of bean populations collected during the field work, visiting the producers or
local markets in central Serbia is extremely significant in bean breeding at the Institute for Vegetable Crops, Smederevska Palanka. In the initial studies of standard
traits of beans: stalk height, number of pods per plant, number of grains in pod,
weight of 1000 grains, shape and colour of grains, potential resistance have been examined. The study covered 37 populations. From all researched traits, this paper
deals with three: stalk height, number of grains per pod and weight of 1000 grains.
Key words: bean, stalk height, number of grains per pod, absolute weight of grain
130
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PROU^AVANJE DIVERZITETA DIVLJE TRE[NJE
(Prunus avium L.) NA PODRU^JU JUGO-ISTO^NE SRBIJE
Evica Mratini}1, Milica Fotiri} Ak{i}1 i Radmila Jokovi}2
1Poljoprivredni fakultet, Zemun
2Institut za {umarstvo, Beograd
U ovom radu ispitivane su fenolo{ke, morfolo{ke i hemijske osobine deset
genotipova divlje tre{anje (Prunus avium L.) razli~ite boje poko`ice, poreklom iz
jugoisto~ne Srbije. Izu~avanjem ove germplazme otkriven je zna~ajan biodiverzitet
izme|u ispitivanih biljaka. Analiza varijanse otktrila je zna~ajne razlike izme|u
genotipova za skoro sve prou~avane osobine. Klaster analizom genotipovi divlje
tre{nje su grupisani i na osnovu pomolo{ke kategorizacije su izdvojene dve grupe.
Prva grupa je sadr`ala dve podgrupe i obuhvatala je sedam genotipova, dok je drugoj
pripadalo samo tri genotipa. Bez obzira na zna~ajne razlike ovaka podela izme|u
prou~avanih biljaka je izvr{ena na osnovu koncentracije ukupnih fenola. Nivo
geneti~kog diverziteta kod prou~avanih genotipova divlje tre{nje je veoma viosk i
zbog toga ova stabla su koristan izvor varijabilnosti za pro~avane osobine i mogu se
koristiti u budu}im oplemenjia~kim programima pri ukr{anju izme|u gajenih sorti i
divljih genotipova.
Klju~ne re~i: pomologija, varijabilnost, analiza varijanse, klaster analiza
ANALYSIS OF WILD SWEET CHERRY (Prunus avium L.) GERMPLASM
DIVERSITY IN THE REGION OF SOUTH-EAST SERBIA
Ten wild growing sweet cherry (Prunus avium L.) genotypes from South-East Serbia
with different fruit skin color were analyzed for its phenological, morphological and
chemical traits. Agronomic evaluation of germplasm accessions revealed considerable diversity among different accessions for all the characters studied. The analysis
of variance revealed significant differences among all genotypes for almost all examined properties. Cluster analysis showed adequate grouping of wild sweet cherry genotypes according to pomological characterization and distinguished them into two
distinct groups. The first group had two subgroups and consisted of seven genotypes,
while the second one included only three accessions. Despite of the significant differences among genotypes, the total concentration of phenols made a clear separation
between the clusters. The level of genetic diversity in these wild sweet cherry genotypes is very high and therefore these trees are useful sources of variability for attributes studied and can be employed in further breeding programs for crosses between
cultivated sweet cherry and wild genotypes.
Kea words: pomology, variability, analysis of variance, claster analysis
131
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
O^UVANJE GENETI^KIH RESURSA DRENA (Cornus mas L.) I SELEKCIJA
PERSPEKTIVNIH GENOTIPOVA ZA [email protected] GAJENJE
Sandra Bijeli}, Branislava Golo{in, Jelena Nini} Todorovi},
Slobodan Cerovi} i Borivoje Bogdanovi}
Poljoprivredni fakultet, Departman za vo}arstvo, vinogradarstvo,
hortikulturu i pejza`nu arhitekturu, Novi Sad
U cilju prou~avanja i o~uvanja prirodnih resursa razli~itih divljih vo}nih vrsta koje
imaju visoko kvalitetne plodove, tokom 2005. god. na Poljoprivrednom fakultetu u
Novom Sadu je intenziviran oplemenjiva~ki program na drenu (Cornus mas L.). Dren
je za{ti}ena samonikla {umska vo}na vrsta koja se mo`e koristiti kao hrana i lek.
Kvalitetna je medonosna biljka pogodna za organsku proizvodnju, sadr`i biolo{ki
aktivne supstance pogodne za ljudsku ishranu, antioksidanse, metabolite zna~ajne za
farmaciju i kozmetiku. Populacije drena u Srbiji uspevaju na razli~itim lokalitetima i
geolo{kim podlogama i izuzetno su veliki i va`an genetski potencijal u oplemenjiva~kom programu. Najve}i problemi u populaciji vezani su za neujedna~en kvalitet i
prikupljanje ploda, {to mo`e vrlo uspe{no da se re{i prelaskom sa sakuplja~ke na
planta`nu proizvodnju drenjina, u skladu sa po{tovanjem principa za{tite `ivotne
sredine i o~uvanjem biodiverziteta. U tom cilju je zasnovan prvi kolekcioni zasad
drena u regionu, kao bitna osnova za o~uvanje geneti~ke divergentnosti, a izdvojene
perspektivne selekcije krupnog ploda i harmoni~nog hemijskog sastava se
umno`avaju i preporu~uju za {irenje na gajenim povr{inama.
Klju~ne re~i: Cornus mas L., varijabilnost, selekcija, genotip, kolekcioni zasad
CONSERVATION OF CORNELIAN CHERRY
(Cornus mas L.) GENETIC RESOURCES AND SELECTION
OF PROMISING GENOTYPES FOR CULTIVATION
In order to study and conservation of various wild fruit species that have high quality
fruit, in 2005. at the Faculty of Agriculture in Novi Sad was intensified cornelian
cherry breeding program (Cornus mas L.). Cornelian cherry is a protected wild forest
fruit species that can be used as food and medicine. That is quality honey plant suitable for organic production, contain biologically active substances suitable for human
consumption, antioxidants, important metabolites for pharmaceutical and cosmetics.
Cornelian cherry population in Serbia succeed in different localities and geological
substrates and are extremely important genetic potential in the breeding program.
The biggest problems in the population are related to the uneven quality and collection of fruit, which can successfully be solved by switching to the plantation production, to the observance of the principles of environmental protection and conservation of biodiversity. To this end, is based the first cornelian cherry genetic pool in the
region with over 30 genotypes for now, as an essential basis for the preservation of genetic diversity, and prospective selection with large fruit and harmonious chemical
composition is recommended to multiply and spread to cultivated areas.
Key words: Cornus mas L., variability, selection, genotype, genetic pool
132
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PROIZVODNE OSOBINE INTRODUKOVANE VRSTE KVINOJA
(Chenopodium quinoa WILL.) U NA[IM AGROEKOLO[KIM USLOVIMA
Slobodan Dra`i}1, \or|e Glamo~lija2 i Sr|an Blagojevi}2
1Institut
za prou~avanje lekovitog bilja „dr Josif Pan~i}“, Beograd
2Poljoprivredni fakultet, Zemun
Kori{}enje alternativnih `ita u ishrani zahteva uvo|enje novih biljnih vrsta u poljoprivrednu proizvodnju, kao {to su kvinoja, amarantus i druga. Kvinoja (Chenopodium
quinoa Will.) vodi poreklo iz Ju`ne Amerike. Osamdesetih godina pro{log veka
introdukovana je u Englesku, a 90-tih godina intenzivirala su se istra`ivanja ove biljne
vrste u zemljama Evropske Unije.
Tokom 2009. i 2010. godine izvo|eni su ogledi na dve lokacije sa introdukovanim
genotipovima kvinoje (KVL 52 i KVL 37). Prose~an prinos zrna ovih genotipova
iznosio je 1.540 kg/ha (KVL 52) i 1.504 kg/ha (KVL 37). Srednje vrednosti za prinos
bile su vi{e u drugoj godini ispitivanja. Variranje prinosa bilo je ne{to vi{e u prvoj
godini (3,8% do 20,7%) u odnosu na iste pokazatelje u drugoj godini (5,3% do 8,8%).
Analiza faktorijalnog ogleda pokazala je da su u prvoj godini ispitivanja, razlike u
visini prinosa zrna nastale slu~ajno. Me|utim, u drugoj godini, ispoljile su se zna~ajne
razlike u prinosu izme|u lokacija i genotipova, {to name}e potrebu daljeg
istra`ivanja.
Klju~ne re~i: genotipovi, godina, lokacija, kvinoja, prinos zrna
PRODUCT TRAITS OF INTRODUCED SPECIES QUINOA (Chenopodium
quinoa WILL.) IN OUR AGROECOLOGICAL CONDITIONS
Use of alternative grains in nutrition requires introduction of new crops in agricultural production, such as quinoa, amaranth and other. Quinoa (Chenopodium quinoa
Will.) originates from South America. It was introduced to England during the eighties, and in 90-years researches on this plant species became intensive in the European
Union.
During 2009 and 2010 experiments were performed at two locations using introduced
genotypes of quinoa (KVL 52 and 37). The average grain yield of these genotypes was
1540 kg / ha (KVL 52) and 1504 kg / ha (KVL 37). Mean yield values were higher in
the second year of research. Yield variations were higher in the first year (3.8% to
20.7%) compared to the same indicators in the second year (5.3% to 8.8%).
Analysis of the factorial experiment showed that in the first year of research, differences in the amount of grain yield occurred by chance. However, in the second year,
significant differences in yield have occurred between locations and genotypes, which
impose the need for further research.
Key words: genotype, year, location, quinoa, grain yield
133
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
MULTIVARIJACIONA ANALIZA VRSTA IZ FAMILIJE Cucurbitaceae
Emina Mladenovi}1, Jano{ Berenji2, Vladislav Ognjanov1, Marija Kraljevi}-Balali}1,
Mirjana Ljubojevi}1, Jelena ^ukanovi}1 i Ivana Blagojevi}1
1Poljoprivredni
fakultet, Departman za vo}arstvo i vinogradarstvo,
hortikulturu i pejza`nu arhitekturu, Novi Sad
2Institut za ratarstvo i povrtarstvo, Novi Sad
Egzoti~ne vrste iz porodice Cucurbitaceae slabo su zastupljene u Srbiji ali s obzirom
na njihove morfolo{ke osobine i dekorativnost zaslu`uju ve}u pa`nju. Cilj rada je bio
prou~avanje varijabilnosti i upotrebne vrednosti deset vrsta iz porodice
Cucurbiataceae. Na osnovu geneti~ke varijabilnosti, vrste su grupisane u 8 klastera.
Osobine ploda kod ve}ine vrsta pokazale su veliku sli~nost dok su se najve}e razlike
pokazale kod osobina cveta i lista. Najdu`i plod bio je kod vrste Trichosanthes
cucumerina (46.2 cm), dok je najkra}i plod imala vrsta Cucumis myriocarpus (3.4 cm)
koja je imala i najmanji obim ploda (4.4 cm). Najve}i obim ploda zabele`en je kod
vrste Cucumis aculeatus (16.4 cm). U pogledu boje ploda izdvojila se vrsta Momordica
balsamina koja je imala plod crvene boje za razliku od ostalih vrsta ~iji su plodovi bili
u razli~itim nijansama zelene. Budu}i da je upotreba ovih vrsta vi{estruka (ishrana
ljudi i stoke, dekorativnost) navedene vrste zaslu`uju posebnu pa`nju u svojoj daljoj
proizvodnji i upotrebi.
Klju~ne re~i: Cucurbitaceae, varijabilnost, upotreba
MULTIVARIATE ANALISYS OF SPECIES FROM Cucurbitaceae FAMILY
Species from Cucurbitaceae family are not widely present in Serbia, although because
of their morphological and decorative features deserve more attention. The aim of
this paper was to study the morphological variability and usage of ten species of the
Cucurbiataceae family. Based on genetic variability, species were grouped into 8 clusters. Fruit characteristics of most investigated species showed great similarity and the
greatest differences were attained for flower and leaf characteristics. The longest was
the fruit of Trichosanthes cucumerina (46.2 cm), while the short est of Cucumis
myriocarpus (3.4 cm) which had the smallest circumference as well (4.4 cm). The largest circumference of fruit was recorded for the species Cucumis aculeatus (16.4 cm). In
terms of fruit color Momordica balsamina had a red fruit, allocated from other species whose fruits were in various shades of green. Variability is reflected in large variations in size, shape and color of fruit. Considerating that usages of these species are
multiple (food for humans and animals, ornamental) studyed species deserve special
attention in their further propagation and use.
Key words: Cucurbitaceae, variability, usage
134
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
STRATEGIJA GENETI^KE KONZERVACIJE MO^VARNOG
TAKSODIJUMA NA VELIKOM RATNOM OSTRVU
Marina Noni}1, Jelena Milovanovi}2 i Mirjana [ija~i}-Nikoli}1
2Fakultet
1[umarski fakultet, Beograd
za primenjenu ekologiju “Futura”, Beograd
Istra`ivanja adaptivnog, proizvodnog i reproduktivnog potencijala taksodijuma
(Taxodium distichum (L.) Rich.) na podru~ju za{ti}enog prirodnog dobra „Veliko
ratno ostrvo“ (VRO) sprovedena su u cilju kreiranja prve strategije i tehni~kih
smernica za konzervaciju geneti~kog diverziteta ove introdukovane vrste. Iako je
taksodijum poreklom iz Severne Amerike, njeno o~uvanje u Srbiji zavre|uje pa`nju,
obzirom da predstavlja jednu od retkih vrsta koje su nosioci vla`nih i periodi~no
plavljenih ekosis tema. Rekognosciranjem terena, evidentirana su 83 stabla
mo~varnog taksodijuma, svako stablo je definisano GPS koordinatama i prate}om
bazom podataka o visini, prsnom pre~niku, morfometrijskim svojstvima {i{arica i
punozrnosti semena. Utvr|eni stepen varijabilnosti posmatranih svojstava populacije
taksodijuma predstavljao je osnovu za definisanje strate{kih ciljeva i metoda
konzervacije. Populacija je uvr{tena u demonstracionu mre`u stani{ta konzervacije
VRO, gde }e, vidno obele`ena, imati, prvenstveno, edukativnu i istra`iva~ku
funkciju. Predlo`eni metod dodatne ex situ konzervacije je osnivanje testova
potomstva, radi daljeg upoznavanja geneti~kog potencijala populacije.
Klju~ne re~i: taksodijum, geneti~ka konzervacija, strategija, tehni~ke smernice
GENETIC CONSERVATION STRATEGY FOR
BALD CYPRESS AT GREAT WAR ISLAND
Bald cypress (Taxodium distichum (L.) Rich.) adaptability, productivity and reproductive potential research in the protected area “Veliko ratno ostrvo” (VRO) have been
conducted with the aim of establishing of the first genetic conservation strategy and
technical guidelines for this introduced species. Although bald cypress is the species
originating from North America, its conservation in Serbia deserves attention, because of its importance for wetland ecosystems sustainability. Through field inventory, 83 individuals of bald cypress are identified, defined with GPS coordinates accompanied with database consisted of tree height, diameter, morpho-metric traits of
cones and seed quality. Recognized intra-population variability level of bald cypress
was baseline for strategic goals and conservation methods definition. Bald cypress
population is included into the demo conservation network of VRO, where it will be
transparently marked and will have, primarily, educational and scientific role. Suggested method of ex situ conservation is establishment of progeny tests with the aim of
further investigation of population genetic potential.
Key words: bald cypress, genetic conservation, strategy, technical guidelines
135
Sekcija / Section 8
Priznavanje i za{tita
sorti i semenarstvo
Approval and protection of plant
varieties and seed production
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
PRAVA OPLEMENJIVA^A BILJNIH SORTI
Gordana Lon~ar, Gordana Tomi}, Jovan Vujovi} i Jan Bo}anski
Ministarstvo poljoprivrede, trgovine, {umarstva i vodoprivrede, Uprava za za{titu bilja, Beograd
Pravo oplemenjiva~a biljne sorte predstavlja intelektualno vlasni{tvo nad sortom i
podrazumeva isklju~ivo pravo na kori{}enje te sorte. Osnovni me|unarodni izvor
pravne za{tite u ovoj oblasti je UPOV Konvencija, koja predvi|a sui generis sistem,
odnosno oblik za{tite intelektualne svojine, posebno prilago|en procesu
oplemenjivanja biljaka, ~iji je cilj podsticanje oplemenjiva~a da razvijaju nove sorte
biljaka. Dono{enjem Zakona o za{titi prava oplemenjiva~a biljnih sorti 2009. godine,
stvorene su realne osnove za za{titu intelektualne svojine na biljnim sortama u
Republici Srbiji. U ovom radu predstavljeni su razlozi za uvo|enje za{tite prava
oplemenjiva~a u skladu sa UPOV Konvencijom, osnovni principi koje nala`e
Konvencija, kao i Zakonom propisani uslovi, na~in, postupak za za{titu i upis u
odgovaraju}e registre.
Klju~ne re~i: UPOV, biljna sorta, pravo oplemenjiva~a
PLANT BREEDERS’ RIGHTS
Plant breeder’s right is a form of intelectual property to the variety and includes the
exclusive right to use this variety. The main source of international legal protection in
this area is the UPOV Convention, which provides for a sui generis system or a form
of intelectual property protection, specifically tailored to the plant breeding, and aims
to ecourage the breeders to develop new varieties. The Law on protection of Plant
Breeders’ Rights, adopted in 2009, has created a realistic basis for intellectual property protection in the Republic of Serbia. The rationale for introduction of the protection of plant breeders’ right in accordance with UPOV Convention, basic principles
laid down by the Convention, as well as the requirements, terms, procedures for the
protection and registration in appropriate registers provided by the Law are described in this paper.
Key words: UPOV, plant variety, breeder`s right
138
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ZA[TITA BILJNE SORTE KAO INTELEKTUALNE SVOJINE
Mirjana Milo{evi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Cilj oplemenjiva~kog rada je stvaranje novih sorti koje }e prihvatiti tr`i{te i koje
donose profit proizvo|a~ima. Oplemenjiva~i svesni modernih zahteva tr`i{ta i
potro{a~a, tra`e mogu}nosti da te zahteve i ostvare. Zbog velikih ulaganja kako
znanja tako i materijalnih sredstava, rezultat oplemenjiva~kog rada, novu biljnu sortu
treba osigurati kao vlasni{tvo. Da bi se oplemenjiva~ima omogu}ila ekonomska
nadoknada za kori{}enje njihovih sorti, uvedena je za{tita biljnih sorti, kao oblik
za{tite prava intelektualne svojine, gde je za{tita usmerena na sortu kao intelektualno
dobro.
Za{tita novih biljnih sorti je specifi~an oblik za{tite intelektualne svojine prisutan u
velikom broju zemalja koje vode ra~una o svojim dostignu}ima na polju stvaranja
novih biljnih sorti. Za{tita nove biljne sorte omogu}ava dodelu ekskluzivnog prava na
eksploataciju novostvorene sorte. Cilj za{tite biljnih sorti je stimulisanje stvaranja
novih sorti i unapre|ivanje kvaliteta proizvoda za potrebe potro{a~a. Kao i u slu~aju
robnih marki i industrijskih patenata, za{tita istra`ivanja i kreativnosti na polju
oplemenjivanja biljaka predstavlja strate{ki va`nu aktivnost svake zemlje.
Klju~ne re~i: sorta, za{tita biljnih sorti, intelektualna svojina
PLANT VARIETY PROTECTION AS INTELLECTUAL PROPERTY
The aim of breeding is to create new varieties which will accept the market and varieties that will make a profit. Breeders are aware of the modern market demands and
customers, looking for opportunities to these demands and achieve. Because of large
investments in knowledge and material resources, the result of breeding, a new plant
variety should be provided as a property. In order to enable breeders economic compensation for the use of their varieties, introduced in the protection of plant varieties,
as a form of intellectual property protection, where protection is focused on variety as
well intellectually.
Protection of new varieties of plants is a specific form of intellectual property protection and it is present in many countries which take care of their achievements in the
creation of new plant varieties. Protection of new plant varieties to award exclusive
rights to exploit newly created variety. The aim of plant variety protection is to stimulate the creation of new varieties and improve product quality for consumers. As in
the case of trademarks and industrial patents, protection of research and creativity in
the field of plant breeding is a strategically important activity of each country.
Key words: variety, plant variety protection, intellectual property
139
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
KONTROLA PROIZVODNJE SEMENA
POLJOPRIVREDNOG BILJA U REPUBLICI SRBIJI
Vlade \okovi} i Jan Bo}anski
Ministarstvo poljoprivrede, trgovine, {umarstva i vodoprivrede, Uprava za za{titu bilja, Beograd
Kontrola proizvodnje semena poljoprivrednog bilja je postupak koji je potrebno
primeniti da bi se potvrdio, odnosno priznao semenski usev. Najva`niji me|unarodni
pravni propisi koji defini{e ovu oblast su OECD {eme (OECD Seed Schemes) i market ing direktive Evropske Unije. U Republici Srbiji ova oblast definisana je
Zakonom o semenu iz 2005. godine, propisima koji su doneti na osnovu njega, kao i
propisima iz oblasti za{tite bilja. U ovom radu predstavljena je zakonska regulativa i
organizacija vr{enja kontrole proizvodnje semena u Republici Srbiji upore|ena sa
me|unarodnim propisima.
Klju~ne re~i: zakonska regulativa, organizacija, vr{enje stru~ne i zdravstvene
kontrole, dokumenti
CONTROL OF AGRICULTURAL SEED
PRODUCTION IN THE REPUBLIC OF SERBIA
Control of agricultural seed production is a procedure that has to be applied in order
to confirm the seed crop, which means to issue a certificat of aknowledgment of the
seed crop. The most important international legal rules that define this area are the
OECD Scheme (OECD Seed Schemes), and marketing directives of the European
Union. In Serbia, this area is defined by the Seed Act of 2005, the regulations enacted
thereunder, as well as regulations on plant protection. This paper presents legislation
and organization of control of seed production in the Republic of Serbia compared
with international regulations.
Key words: legislation, organization, conduct of professional and health check, documents
140
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POSTKONTROLNI TESTOVI KAO JEDAN OD NA^INA
UNAPRE\ENJA SEMENARSTVA U SRBIJI
Vladimir Sabado{1, Jan Bo}anski2 i Dragan Dedi}2
2Ministarstvo
1PSS „Sombor“ doo, Sombor
poljoprivrede, trgovine, {umarstva i vodoprivrede, Uprava za za{titu bilja,
Beograd
Kontrola proizvodnje kao i certifikacija semena poljoprivrednog bilja regulisana je u
republici Srbiji Zakonom o semenu iz 2005. godine, kao i propisima koji su doneti na
osnovu njega. Ovim dokumentima se reguli{e i postkontrolni test, kao obavezna mera
koja je obavezna u ve}ini zemalja Evropske unije. U ovom radu predstavljena su prva
iskustva i rezultati postkontrolnih testova, obavljenih u periodu od 2006. do 2011.
godine, i to na slede}im biljnim vrstama: ozima i jara strna `ita, kukuruz, suncokret,
uljana repica, soja, {e}erna repa, kao i crni luk proizveden iz arpad`ika. Tako|e su u
ovom radu osim rezultata dati predlozi i sugestije kako da se ovaj seg ment u
certifikaciji semena jo{ kvalitetnije obavlja, a sve u cilju za{tite krajnjih korisnika
semena, to jest poljoprivrednih proizvo|a~a.
Klju~ne re~i: postkontrolni test, certifikacija semena, zakon, propis
POSTCONTROL TESTS AS ONE OF WAYS TO
PROMOTE SEED PRODUCTION IN SERBIA
Control of production and certification is regulated in Serbia by Low of seed from
year 2005, and with regulations connected to this low. With this regulations also is
regulated postcontrol testing as obligatory, same is in most countries in EU. In this
paper work we present first experiences and results of post control tests, testing was
during 2006-2011 period on this species:winter and spring small grains, corn, sunflower, oil seed rape, soya sugar beet and also red onion produced from onion set. In
this paper work, beside results we give proposals and suggestions how to work with
more quality in this segment in seed certification, final goal is to protect users of seed
which are farmers.
Key words: post control test, seed, certification, low, regulations
141
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
POLJSKO NICANJE RAZLI^ITIH GENOTIPOVA SUNCOKRETA
Jelena Mr|a, Bo{ko Dedi} i Vladimir Mikli~
Institut za ratarstvo i povrtarstvo, Novi Sad
U radu je ispitan uticaj razli~itog hemijskog tretiranja i uslova ~uvanja semena na
poljsko nicanje sedam genotipova suncokreta. Poljsko nicanje je ispitano neposredno
nakon tretiranja i godinu dana nakon tretiranja i ~uvanja semena u skladi{tu i hladnoj
komori. Ogled je bio postavljen po split-split-plot metodu. Dobijeni podaci statisti~ki
su obra|eni analizom varijanse trofaktorijalnog ogleda. Poljsko nicanje semena
ispitivanih genotipova bilo je u proseku za ceo ogled 69,42%. Svi genotipovi su imali
visoko signifikantno ve}e poljsko nicanje od linija. Kod hibrida NS-H-111 (88,80%) je
bilo visoko signifikantno najve}e poljsko nicanje, a kod hibrida Rimi (73,59%) visoko
signifikantno najmanje. Iz interakcije pri istom hemijskom tretmanu i razli~itim
uslovima ~uvanja uo~ava se da je u kontroli i kod tretmana benomil + metalaksil i
fludioksonil + metalaksil poljsko nicanje semena iz skladi{ta bilo visoko signifikantno
najve}e. U kontroli i kod tretmana fludioksonil + metalaksil i fludioksonil +
metalaksil + imidakloprid poljsko nicanje sve`eg semena je bilo visoko signifikantno
ve}e od semena iz hladne komore. Kod interakcije pri istim uslovima ~uvanja i
razli~itom hemijskom tretmanu uo~ava se da kod semena iz skladi{ta izme|u
hemijskih tretmana zna~ajnih razlika nije bilo.
Klju~ne re~i: suncokret, nicanje, tretiranje, ~uvanje
FIELD EMERGENCE OF DIFFERENT SUNFLOWER GENOTYPES
The objective of this study was to assess the impact of different chemical treatments
and storage conditions on field emergence of seven sunflower genotypes. Field emergence was examined immediately after treatment and one year after treatment and
storage of seed in warehouse and cold storage. The experiment was set up by the
split-split-plot method. The data were statistically analyzed by analysis of variance the
three-factorial experiment. Field emergence of genotypes was on average 69.42% for
whole experiment. All genotypes had significantly higher field emergence then line.
Hybrid NS-H-111 (88.80%) had significantly highest field emergence and hybrid
Rimi (73.59%) significantly lowest. From the interaction of the same chemical treatment and various storage conditions can be seen that the control and the treatments
benomil + metalaxyl and fludioxonil + metalaxyl field emergence of seeds from storage were sig nif i cantly high est. In the control and treatment with fludioxonil +
metalaxyl and fludioxonil + metalaxyl + imidacloprid field emergence of fresh seeds
was highly significantly higher than seeds from the cold chamber. Observing the interaction with the same storage conditions and different chemical treatment can be seen
that the seeds from storage were no significant differences between the chemical
treatments.
Key words: sunflower, field emergence, treatment, storage
142
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ [email protected] VLAGE NA KVALITET
SEMENA STO^NOG GRA[KA (Pisum sativum L.)
Branko Milo{evi}, \ura Karagi}, Sanja Vasiljevi},
Aleksandar Miki}, Dragan Mili} i Vojislav Mihailovi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Ispitivan je uticaj vla`nosti semena u momentu `etve na najva`nije parametre
kvaliteta semena sto~nog gra{ka (Pisum sativum L.) od faze formiranja do pune
zrelosti. Utvr|ena je energija klijanja, klijavost, udeo atipi~nih ponika i masa semena
kod tri genotipa sto~nog gra{ka. @etva je obavljena ru~no, kako bi se izbegla
mehani~ka o{te}enja semena. Ura|eno je 20 tretmana. Prva `etva obavljena je sa
sadr`ajem vlage u semenu od 77,67%, a poslednja sa 10,33%. Energija klijanja je
iznosila od 0 do 99%, a klijavost od 0 do 100%. Broj atipi~nih ponika se kretao od 0
do 7%.
Klju~ne re~i: vla`nost semena, energija klijanja, klijavost, sto~ni gra{ak (Pisum sativum L.)
THE INFLUENCE OF MOISTURE CONTENT
ON FIELD PEA (Pisum sativum L.) SEED QUALITY
The effect of seed moisture at the time of harvest on most important parameters of
seed quality of field pea from formation stage until full maturity stage was investigated. Germination energy, germination, seedling and atypical proportion of seed
mass in three genotypes of field pea were determined. Pea harvest is done manually,
in order to avoid mechanical damages to the seeds. It was done 20 treatments. The
first harvest was carried out with a 77.67% moister content in seed and last one with
10.33%. Germination energy ranged from 0 to 99% and the germination from 0 to
100%. Number of abnormal seedlings ranged from 0 to 7%.
Key words: Seed moisture content, germination energy, germination, field pea (Pisum
sativum L.)
143
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UTICAJ ZASLANJENOSTI NA OKSIDATIVNI
STRES KOD ULJANE REPICE (Brassica napus L.)
Du{ica Jovi~i}, Ana Marjanovi} Jeromela, Zorica Nikoli},
Maja Ignjatov, Dragana Milo{evi} i Gordana Zdjelar
Institut za ratarstvo i povrtarstvo, Novi Sad
Prvi stepen }elijskog odgovora na oksidativni stres je antioksidantna odbrana i aktiviranje sistema za obnavljanje }elijskih struktura o{te}enih dejstvom slobodnih
radikala. Odre|ivanje antioksidantnog kapaciteta mo`e imati veliki zna~aj jer se
merenjem intenziteta aktivnosti antioksidantnih enzima, lipidne peroksidacije i
ostalih parametara antioksidantnog kapaciteta, mogu utvrditi razlike u tolerantnosti
biljnih vrsta i sorti prema salinitetu. Ispitivanje je obuhvatilo ~etiri genotipa uljane
repice (Bana}anka, Jasna, Kata i Zlatna) stvorenih u Institutu za ratarstvo i
povrtarstvo u Novom Sadu. U laboratorijskim uslovima simulirani su uslovi
zaslanjenosti tako {to su u podloge za naklijavanje kva{ene rastvorom NaCl
koncentracija 0, 100, 150 i 200 mM. Kao pokazatelji oksidativnog stres, u klijancima
starim 21 dan ispitivani su intenzitet lipidne peroksidacije, aktivnost antioksidativnih
enzima gvajakol-peroksidaze i superoksid-dismutaze. Rezultati ovog istra`ivanja
pokazuju da zaslanjenost zemlji{ta prouzrokuje stvaranje slobodnih radikala, {to za
posledicu ima pove}an intenzitet lipidne peroksi dacije i oksidativnog stresa u
biljkama.
Klju~ne re~i: antioksidantni status, oksidativni stres, uljana repica, zaslanjenost
THE EFFECT OF SALINITY ON OXIDATIVE
STRESS IN OILSEED RAPE (Brassica napus L.)
First reaction to oxidative stress on a cellular level is antioxidant defence system and
activation the system for cell structure regeneration damaged by free radicals activity.
The antioxidant capacity determination can be of great importance because measuring the intensity of antioxidant enzymes activities, lipid peroxidation and antioxidant
capacity of the other parameters can determine differences in salt tolerance in many
species and cultivars. The study included four genotypes of seed rape (Bana}anka,
Jasna, Kata i Zlatna) bred in the Institute of Field and Vegetables Crops in Novi Sad.
In laboratory conditions, the salinity stress was simulated by adding different concentrations (0, 100, 150, 200 mM) of a NaCl solution to the growing media. As indicator
of oxidative stress, intensity of lipid peroxidation, activity of guaiacol peroxidase and
superoxid dismutase were examined in 21-old-days seedlings. The results of this study
indicate that soil salinity causes formation of free radicals reflected in increased intensity of lipid peroxidation and oxidative stress in plants.
Key wors: antioxidant status, oxidative stress, canola, salinity
144
Sekcija / Section 9
Prikaz novopriznatih sorti
Review of new plant cultivars
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ZP 560 NOVI HIBRID KUKURUZA
Milomir Filipovi}, Milan Stevanovi}, Goran Stankovi}, Zoran ^amd`ija,
Sne`ana Mladenovi} Drini}, Jovan Pavlov i Vesna Kandi}
Institut za kukuruz „Zemun Polje”, Beograd
Institut za kukuruz Zemun Polje je kroz svoj decenijski rad uspeo da isprati svetske
trendove u proizvodnji hibrida kukuruza. Jedan od poslednje stvorenih u bogatoj
paleti hibrida je hibrid ZP 560. Ovaj hibrid ima sve odlike jednog modernog hibrida.
Spada u grupu srednje kasnih hibrida i odlikuje se visokim i stabilnim prinosima i
brzim otpu{tanjem vlage. Jedna od njegovih glavnih odlika jeste tolerancija na stresne
uslove su{e. Morfologija hibrida je takva da on ima nisko, tanko i elasti~no stablo.
Klip je ni`e nasa|en na stabljici, konusnog je oblika i ima 14-16 redova zrna. Listovi u
odnosu na stabljiku imaju erektofilan (uspravan) polo`aj {to omogu}ava gajenje ovog
hibrida u ve}im gustinama i intenzivnim uslovima proizvodnje. Zanimljivo je
napomenuti i to da hibrid ima tvr|e zrno i ve}i sadr`aj proteina u odnosu na standard.
Hibrid ZP 560 ispitivan je u ogledima sortne komisije u periodu 2008-2009. godine.
Tokom ispitivanja dao je visoko signifikantno vi{i prinos u odnosu na standard.
Klju~ne re~i: hibrid, kukuruz, prinos
ZP 560 - A NEW MAIZE HYBRID
Through the many decades of its activities, the Maize Research Institute Zemun
Polje, has managed to catch up on global trends in maize hybrid production. The ZP
560 hybrid is one of the most recently developed hybrids in a rich palette of maize hybrids. This hybrid has all properties of a modern hybrid.
It belongs to a group of medium late maturity hybrids and it is characterised with high
and stable yields and a fast dry down rate. Tolerance to stress conditions of drought is
one of its main traits. Its morphological properties are low, thin and elastic stalk. The
lowly inserted ear is conical with 14-16 kernel rows. As leaves are erect, it is possible
to grow this hybrid in greater sowing densities and under intensive production conditions. It is interesting to mention that this hybrid has harder kernels and higher protein content than the check.
The ZP 560 hybrid was tested in the official trials of the Commission for the Variety
Releasing during 2008-2009 periods. During its testing, the hybrid had highly significantly higher yield than the check.
Key words: hybrid, maize, yeald
146
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
ZP 427 - NOVI HIBRID KUKURUZA
Milomir Filipovi}, Zoran ^amd`ija, Jovan Pavlov, Sofija Bo`inovi},
Nikola Gr}i}, Milan Stevanovi} i Milo{ Crevar
Institut za kukuruz „Zemun Polje”, Beograd
Savremeni trendovi u svetu, kada je u pitanju merkantilna proizvodnja kukuruza,
podrazumevju sve ve}e u~e{}e srednje ranih hibrida koji se odlikuju visokim i
stabilnim prinosima sa brzim otpu{tanjem vlage iz zrna. Institut za kukuruz Zemun
Polje, imaju}i u vidu aktuelne trendove u svetu i u na{oj zemlji, intenzivirao je program stvaranja hibrida sa prethodno navedenim osobinama. Iz takvog programa
proistekla je nekolicina hibrida, me|u kojima se posebno isti~e hibrid ZP 427. Pored
ve}eg prinosa i ni`e vlage u vreme berbe, novi hibrid ima moderniju biljku, tj. biljka je
ni`a sa nisko nasa|enim klipom, stablo tanje, veoma ~vrsto i elasti~no. Klip je
cilindri~nog oblika u proseku sa 16 redova zrna. Zahvaljuju}i erektofilnim listovima,
omogu}eno je gajenje ovog hibrida u ve}im gustinama i intenzivnim uslovima
proizvodnje.
ZP 427 - NEW MAIZE HYBRID
Modern trends in the world when it comes to maize production, means increasing the
participation of early medium hybrids, that are characterized by high and stable yields
with a great grain dry-down rates from grain. Maize Research Institute Zemun Polje,
given the current trends in the world and our country, intensified program to create a
hybrid with the aforementioned characteristics. From this programme several hybrids
came out, among which hybrid ZP 427 stands out. In addition to higher yields at lower
moisture content at harvest time, the new hybrid has a more modern plant, ie. plant is
lower with lower positioned ear, stalk is thiner, very firm and elastic. Ear is cylindrical
in shape with an average of 16 rows of kernels. Thanks to erect top leaves, the cultivation of hybrid in higher densities and intensive production conditions are enabled.
147
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NOVI HIBRIDI KUKURUZA [E]ERCA
INSTITUTA ZA KUKURUZ „ZEMUN POLJE“
Jelena Srdi}, Zorica Paji}, Milica Radosavljevi} i Marija Mila{inovi} [ereme{i}
Institut za kukuruz „Zemun Polje“, Beograd
Od 70tih godina pro{log veka kada je u Institutu za kukuruz „Zemun Polje“ zapo~et
program oplemenjivanja kukuruza {e}erca, stvoreno je 32 hibirda {e}erca razli~itih
grupa zrenja. Cilj oplemenjivanja kukuruza {e}erca je stvaranje hibrida visokog
prinosa i tehnolo{kog kvaliteta. Zbog toga se posebna pa`nja obra}a osobinama koje
determini{u ukus - sadr`aj {e}era, sadr`aj suve materije, so~nost zrna, ne`nost perikarpa, kao i izgled klipa i zrna - zdravstveno stanje, raspored redova zrna, ozrnjenost,
oblik i veli~ina klipa, {irina i dubina zrna. Na osnovu dvogodi{njih komisijskih ogleda
u 2010 priznata su dva hibirda kukuruza {e}erca ZP 484su i ZP 545su, a u 2011 - ZP
561su. Ova tri hibirda tehnolo{ku zrelost dosti`u za 80 dana od nicanja. U
komisijskim ogledima ostvarili su zna~ajno vi{e prinose od standarda ZP 504su (10.80
t/ha), od 12.33 tha (ZP 545su), 12.65 (ZP 561su) i 14.43 t/ha (ZP 484su). Novi hibridi
{e}erca zasnovani su na recesivnom alelu su i odlikuju se pove}anim sadr`ajem {e}era
u zrnu, so~nim zrnom i mekim perikarpom. Oblik i izgled klipa odgovara zahtevima
industrijske proizvodnje i prerade, zbog ujedna~enosti klipa, rasporeda redova zrna i
dubine zrna, ali tako|e se mogu koristiti i za sve`u potro{nju. Sve ovo je prikazano
ukupnom ocenom kvaliteta ovih hibrida koja je bila vi{a od 95 %.
Klju~ne re~i: kukuruz {e}erac, prinos, ocena kvaliteta
NEW SWEET CORN HYBRIDS DEVELOPED AT
MAIZE RESEARCH INSTITUTE “ZEMUN POLJE”
Since 1970’s when breeding of sweet corn was initiated at Maize Research Institute
“Zemun Polje”, 32 sweet corn hybrids of different maturity groups were developed.
The aim of its breeding is creation of high yielding and high quality hybrids. Therefore special attention is focused on traits determining taste - dry matter content, sugar
content, pericarp tenderness, creaminess, and ear and kernel appearances - resistance
to diseases, kernel row configuration, seed set, size and shape of the ear, kernel width
and depth. Based on the two year official trials of the Committee for the Release of
Varieties in 2010 two sweet corn hybrids ZP 484s and ZP 545su and in 2011 ZP 561su,
were released. Those three hybrids reach technological maturity in 80 days after
emerging in the field. New hybrids produced significantly higher yields than the standard hybrid ZP 504su (10.80 t/ha), ZP 545su - 12.33 t/ha, ZP 561su - 12.65 t/ha and ZP
484su - 14.43 t/ha. In their genetic base those hybrids contain recessive su allele so
they are featured with higher sugar content, creamy kernel and soft pericarp. Ear size
and shape is corresponding to the industrial processing purposes, due to the ear uniformity, kernel set, configuration and depth. On the other hand those new hybrids are
desirable in fresh market as much. Those traits are evaluated through total quality
points that were above 95% for all three hybrids.
148
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NS HIBRID SUNCOKRETA - VLADIMIR
Radovan Marinkovi}, Milan Jockovi}, Ana Marjanovi} Jeromela,
Velimir Radi} i Nada Le~i}
Institut za ratarstvo i povrtarstvo, Novi Sad
Vladimir je srednje rani hibrid, du`ine vegetacije oko 120 dana. Stabljika je ~vrsta,
visine 165-175 cm. Poseduje genetski potencijal za prinos semena od oko 6t/ha i
sadr`aj ulja u semenu od oko 50%. Spada me|u standardne genotipove suncokreta
jer se karakteri{e visokim sadr`ajem linolne masne kiseline (18:0) u ulju (52,49%).
Genetski je otporan prema plamenja~i (Pl1 i Pl2 gen), r|i, suncokretovom moljcu ,
pet rasa volovoda (A, B, C, D, E) i visoko tolerantan prema Phomopsis sp. Atraktivan
je za polinatore, veoma dobro podnosi su{u i mo`e se gajiti na razli~itim tipovima
zemlji{ta. Optimalan sklop je 53-55000 biljaka po hektaru.
Klju~ne re~i: oplemenjivanje, inbred linija, restorer, lokalitet, sadr`aj ulja
NS HYBRID OF SUNFLOWER - VLADIMIR
Vladimir is a medium early hybrid with vegetation period about 120 days. Stem is
strong with 165-175 cm of height. Genetic potential for seed yield is about 6t/ha with
seed oil content at about 50%. It belongs to standard sunflower genotypes because of
high content of linoleic fatty acid (18:0) in oil (52,49%). It has genetic resistance to
downy mildew (Pl1 and Pl2 gene), sunflower rust, moth and five races of broomrape
(A, B, C, D, E) and high tolerance to Phomopsis sp. It is attractive to pollinators, well
tolerated on drought and can be grown in different soil types. Optimum density is
about 53-55000 plants per hectare.
Key words: breeding, inbred line, restorer, locality, oil content
149
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
NOVA SORTA BOSILJKA
Slobodan Dra`i}
Institut za prou~avanje lekovitog bilja „Dr Josif Pan~i}“, Beograd
Re{enjem Ministarstvo poljoprivrede, {umarstva i vodoprivrede Republike
Srbije,broj: 320-09-39/134/2-2005-06. os 21.09.2005.godine, priznata je sorta bosiljka
(Ocimum basilicum L.) pod nazivom: Miki. Ova sorta razvija polu`bun (visine oko 60
cm) koji se grana, sa dosta listova i izdu`enim cvastima na vrhovima grana. Stablo je
uspravno, zeleno, ~etvorougaono a grananje dihotomo. Cvast je cimozna, du`ine
15-20 cm. Na biljci se formira vekili broj cvasti.
Od sadnje do cvetanja i prve `etve treba 70-80 dana, a do druge `etve 120-130 dana,
{to ukazuje da je ova sorta poznija u odnosu na standard (Sitnolisni). Prinos
nadzemnog dela (herba) je vi{i za 20-30%. Sadr`aj etarskog ulja u ovoj sorti, kre}e se
oko 0,8%, a kod standarda oko 0,4 %. Sorta pripada linalolskom tipu.
Klju~ne re~i: bosiljak, etarsko ulje, linalolski tip, prinos
NEW CULTIVAR OF BASIL
The Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia released a new cultivar of basil (Ocimum basilicum L.) named Miki by the Resolution No. 320-09-39/134/2-2005-06 of Sep tem ber 21, 2005. This cultivar de vel ops
semi-shrub (up to 60 cm) that branches, has many leaves and elongated inflorescences
at the top of branches. The stalk is erect, green, square, with dichotomic branching.
The length of cymose inflorescence amounts to 15-20 cm. A great number of inflorescences are formed on the plant.
The period from mid-flowering to the first, i.e. second harvest lasts 70-80, i.e. 120-130
days, respectively, indicating that this cultivar is of a later maturity than the check
(Sitnolisni). The yield of the above ground part is higher by 20-30%. The content of
essential oil in this cultivar amounts to ap proximately 0.8%, while in the check
amounts to about 0.4%. The cultivar belongs to a linalol type
150
Okrugli sto / Round table
Kvalitet hrane - doprinos nauke
Food quality - contribution of science
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
SAVREMENI PRAVCI RAZVOJA POLJOPRIVREDE
Du{an Kova~evi}1,3 i Branka Lazi}2,3
1Poljoprivredni fakultet, Zemun
2Poljoprivredni fakultet, Novi Sad
3Akademija
in`enjerskih nauka Srbije
Budu}nost poljoprivrednog razvoja u XXI Veku podrazumeva odr`ivu poljoprivredu
kao alternativu sada{njoj konvencionalnoj. Poljoprivreda je obi~no razvijena onoliko
koliko i samo dru{tvo u kome postoji kao privredna grana. Danas postoje razli~iti
pravci na kojima je zasnovana poljoprivreda, od najvi{e zastupljene vrlo intenzivne
kon ven cionalne poljoprivrede, do brojnih ekolo{kih pravaca zasnovanih na
ekolo{kim principima.
Konvencionalna poljoprivreda ima zadatak da obezbedi maksimalnu proizvodnju u
pogledu kvantiteta i kvaliteta uz {to manje tro{kove. Za te svrhe koristimo brojne
agrotehni~ke mere, koje ponekad, pored o~ekivanih pozitivnih, imaju mnoge
negativne dugoro~ne efekte u agroekosistemima i u ekosistemu u celini.
Organska poljoprivreda je jedan od interesantnih aktuelnih pravaca zasnovan na ekolo{kim principima i na odsustvu primene agrohemikalija. Zahvalju}i sveobuhvatnom
pristupu organskom proizvodnjom dobija se bolji kvalitet proizvoda (bolji ukus,
pove}an sadr`aj suve materije, vitamina i drugih antioksidantnih materija), za{tita
prirodnih resursa (zemlji{te, voda) i pove}anje biodiverziteta.
Klju~ne re~i: odr`ivi razvoj, konvencionalna, organska poljoprivreda
CONTEMPORARY DIRECTIONS OF AGRICULTURE DEVELOPMENT
Future of agriculture development in the XXI Century will imply sustainable agriculture as the alternative to the industrial agriculture. Agriculture is usually developed
as much and just society where there is a branch of the economy. Today, there are different directions from industry agriculture to many concepts based on ecological principles.
Conventional agriculture as an intensive has a duty to ensure maximum production in
terms of quantity and quality with the low cost. For this purpose we have many cultural practices, sometimes in addition to the expected positive but sometimes with
many unexcepted long-term negative effects in agroecosystems.
Organic agriculture is one of the most interesting current trends in agriculture completely based on strong ecological principles and the absence of application of agrochemicals (pesticides, fertilizers, hormones), GMO, etc. Organic agriculture is a holistic way of farming: besides production of goods of high quality (better flavor, high
content dry matter, vitamins, antioxidants); conservation of the natural resources
(soil,water) and richness biodiversity.
Key words: sustainable development, conventional agriculture, organic farming
152
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
[email protected] RAZVOJ I ORGANSKA POLJOPRIVREDA
Mirjana Milo{evi}
Institut za ratarstvo i povrtarstvo, Novi Sad
Grupa ekonomista okupljena u Svetskoj komisiji za `ivotnu sredinu i razvoj je 1987.
godine uvela u svetsku ekonomiju pojam odr`ivog razvoja. Ovaj novi koncept razvoja
ljudskog dru{tva uveden je zbog potrebe da se preispita dosada{nji koncept u
ekonomiji, koji se zasniva na stalnom pove}anju stope rasta i profita. Posledice
takvog pona{anja su veoma negativne, naro~ito po neobnovljiva prirodna bogatstva,
kakva su zemlji{te, voda i vazduh.
Odr`ivi razvoj je nova, op{te prihva}ena koncepcija razvoja ljudskog dru{tva koja se
zasniva na razvoju koji ne}e ostaviti negativne posledice na `ivotnu sredinu i prirodu.
Uvo|enjem ekolo{kih principa u proizvodnju hrane vr{i se prelaz iz konvencionalne
poljoprivrede u alternativnu ili odr`ivu koja je mnogo prihvatljivija za `ivotnu
sredinu.
Odr`ivi razvoj je skladan odnos ekologije i privrede, kako bi se prirodno bogatstvo
na{e planete sa~uvalo i za budu}e nara{taje. Mo`e se re}i da odr`ivi razvoj predstavlja
op{te usmerenje, te`nju da se stvori bolji svet, balansiraju}i socijalne, ekonomske i
~inioce za{tite `ivotne sredine.
Danas je na globalnom nivou sagledana potreba o~uvanja `ivotnih resursa kroz
odr`ivi na~in proizvodnje uz upotrebu najmanje {tetnih tehnologija. Jedno od
prihvatljivih re{enja je sistem odr`ive poljoprivrede koji kao osnovu ima primenu
principa organske proizvodnje.
Klju~ne re~i: odr`ivi razvoj, organska proizvodnja
SUSTAINABLE DEVELOPMENT AND ORGANIC AGRICULTURE
A group of economists assembled by the World Commission on Environment and
Development in 1987. introduced into the global economy, the concept of sustainable
development. This new concept of development of human society has been introduced because of the need to review the existing concept of the economy, which is
based on the constant increase in the rate of growth and profits. The consequences of
this behavior are very negative, especially on non-renewable natural resources such
as land, water and air.
Sustainable development is a new, generally accepted concept of the development of
human society based on development which will leave a negative impact on the environment and nature. The introduction of ecological principles in food production
made the transition from conventional agriculture or sustainable alternative that is
much more acceptable for the environment.
Sustainable development is a harmonious balance between ecology and economy, to
the natural resources and preserve our planet for future generations. It can be said
that sustainable development is a general orientation, the desire to create a better
world, balancing social, economic and environmental factors.
Today is globally perceived need to preserve vital resources through sustainable production, using the least damaging technologies. One acceptable solution is a system of
sustainable agriculture as a basis the application of the principles of organic production.
Key words: sustainable development, organic farming
153
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
INTEGRALNA I ORGANSKA PROIZVODNJA JABUKE - BUDU]NOST
POLJOPRIVREDE I VO]ARSTVA SRBIJE
Zoran Keserovi}1, Marko Injac2, Nenad Magazin1, Biserka Mili}1 i Marko Dori}1
1Poljoprivredni fakultet Novi Sad
2Chemical Agrosava, Beograd
Integralni koncept proizvodnje vo}a omogu}ava dobijanje visokih prinosa kvalitetnih
i zdravstveno ispravnih plodova. U odnosu na konvencionalnu, integralna proizvodnja je ekolo{ki prihvatljiva jer nala`e poseban pristup za{titi, ishrani i tehnologiji
proizvodnje vo}a. Da bi se obezbedio plasman na Evropsko tr`i{te neophodno je
intezivirati uvo|enje integralnog koncepta proizvodnje vo}a u Srbiji. U radu je
predstavljen integralni koncept proizvodnje jabuke u zasadu „ATOS VINUM“ u
Maloj Remeti na Fru{koj gori.
U integralnoj proizvodnji dozvoljena je strogo kontrolisana upotreba sinteti~kih
hemijskih sredstava, navedenih u Uputstvu Radne grupe za integralnu proizvodnju
jabuke Ju`nog Tirola (AGRIOS). U organskoj proizvodnji, primena sinteti~kih
hemij skih sredstava nije dozvoljena. Ovakvim pristupom elemini{e se svaka
potencijalna opasnost od primene hemijskih siteti~kih sredstava. Principi organske
proizvodnje nala`u uvo|enje sorti jabuke koje poseduju genetsku otpornost prema
parazitima. Ovakav na~in proizvodnje u Srbiji je veoma slabo zastupljen, te se u radu
razmatra zna~aj organske proizvodnje i dat je pregled osnovnih elementa organske
proizvodnje jabuke.
Klju~ne re~i: za{tita vo}aka, ishrana vo}aka, prinos, kvalitet plodova, otporne sorte
jabuke
INTEGRATED AND ORGANIC APPLE PRODUCTION - THE FUTURE
OF SERBIAN AGRICULTURE AND FRUITGROWING
Implementation of Integrated apple production provides high yields of high quality
and health safe fruits. If compared to the conventional fruit production, integrated
production is environmentally acceptable because it sets specific approach to plant
protection, nutrition and production technology. In order to provide apple export on
European market, implementation of integrated production in Serbia, on the large
scale is needed. The paper presents integrated apple production in plantation of
„ATOS VINUM“company, located in Mala Remeta on Fruska Gora.
Integrated fruit production allows strictly controlled application of synthetic chemicals, which are listed in Guidelines for integrated pome production (AGRIOS). On
contrary, application of any synthetic chemicals in organic apple production is not allowed. Such approach eliminates any potential risk of chemicals application to the environment. Organic production principles point out the importance of apple cultivars
with genetic resistance to pests and diseases. Because organic apple production in
Serbia is very limited, the purpose of the paper is to point out the importance of organic concept and review of its basic principles.
Key words: apple trees protection, fertilization, yield, fruit quality, resistant apple
cultivars
154
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
UPOTREBA SOJE I KUKURUZA KAO IZVORA ZDRAVSTVENO
KORISNIH SUPSTANCI U PROIZVODNJI FUNKCIONALNE HRANE
Sne`ana Mladenovi} Drini}, Sla|ana @ili}, Marija Mila{inovi} i Mirjana Srebri}
Institut za kukuurz “Zemun Polje”, Beograd, Srbija
U savremenom dru{tvu je sve izra`enija pove}ana potra`nja hrane koja osim
osnovnih hranljivih materija sadr`i i supstance koje smanjuju rizik od nastanka nekih
bolesti. Zdravstveni efekti delimi~no se pripisuju {irokom spektaru fitohemikalija, te
kukuruz i soja kao izvori velikog broja ovih komponenti imaju sve ve}i zna~aj u
proizvodnji funkcionalne hrane. Soja je zna~ajan izvor polinezasi}enih esencijalnih
masnih kiselina koje su prekurzori biolo{ki aktivnih supstanci, eikozanoida, koje
reguli{u funkcije mozga povezane sa spavanjem, termoregulacijom i odgovorom na
bolne nadra`aje. Daidzein i genistein su najzna~ajniji izoflavoni u soji, i kao
fitoestrogeni u menopauzi kod `ena koriste se kao regulatori nivoa hormona.
Genistein je inhibitor nekoliko klju~nih enzima koji u~estvuju u karcinogenezi.
Fenolne komponente prisutne u hrani smanjuju rizik od pojave hroni~nih bolesti
redukcijom oksidativnog stresa. Antocijaninini prisutni u crvenom i plavom
kukuruzu, kao i crnoj soji pored antioksidativnog efekta imaju ulogu i u redukciji
rizika pojave dijabetesa. S obzirom na zna~aj upotrebe kukuruza i soje u proizvodnji
hrane, selekciji genotipova sa pove~anim sadr`ajem zdravstveno-korisnih supstanci u
budu}e bi trebalo treba posvetiti vi{e pa`nje.
Klju~ne re~i: soja, kukuruz, fitohemikalije, funkcionalna hrana
SOYBEAN AND MAIZE AS A SOURCE OF HEALTH BENEFICIAL
SUBSTANCES IN THE PRODUCTION OF FUNCTIONAL FOODS
In contemporary society is increasing demand for foods that beside basic nutrients
containing substances that reduce the risk of some diseases. Health effects are partly
attributed to a wide range of phytochemicals, so maize and soybeans as a source of
many of these components are of increasing importance in functional foods production. Soy is an important source of polyunsaturated essential fatty acids that are precursors of biologically active substances, eicosanoids, which regulate brain function
associated with sleep, thermoregulation and response to painful stimuli. Daidzein and
genistein are the most important isoflavones in soybeans, and as phytoestrogens are
used as hormone regulators in menopause. Genistein is an inhibitor of several key enzymes involved in carcinogenesis. Phenolic components present in food reduce risk of
chronic disease by reduction of oxidative stress. Anthocyanins present in red and blue
maize, and black soybeans in addition to antioxidant effects have a role in reducing
the risk of diabetes. Since the importance of using maize and soybean in food production, selection of genotypes with increased content of health beneficial substances in
the future should be given more attention.
155
ZBORNIK ABSTRAKATA
IV SIMPOZIJUMA SEKCIJE ZA OPLEMENJIVANJE ORGANIZAMA DRU[TVA GENETI^ARA SRBIJE
Kladovo, 2-6. oktobar 2011.
GENETI^KI MODIFIKOVANI ORGANIZMI (GMO) - RIZICI
I DOBITI U ISHRANI LJUDSKE POPULACIJE
Kosana Konstantinov1,2 i Sne`ana Mladenovi} Drini}1
1Institut za kukuruz“Zemun Polje”, Beograd
2Akademija in`enjerskih nauka Srbije, Beograd
Razvoj transgenih organizama je postao rutina u poslednje dve decade i izmenio je u
osnovi prirodu oplemenjivanja biljaka. Mogu}nost transgenih biljaka da ubla`e glad u
svetu i mogu}i efekti na ljudsko zdravlje su u radu diskutovani. Ishrana ljudske
populacije od deset milijardi koja se predvi|a u bliskoj budu}nosti pretstavlja eti~ko
opravdanje kori{}enja te biotehnologije. Rizici i koristi u bezbednosti hrane i `ivotne
sredine koje proizilaze iz transformacije geneti~kim in`enjeringom biljaka na
rezistentnost na bioti~ki i abioti~ki stres kao i efekti na biodiverzitet }e biti
razmatrani. Dileme koje su proiza{le kao rezultat razvoja i kori{}enja transgenih
biljaka kao; i) da li transgene biljke pretstavljaju re{enje za glad u svetu; ii)
neprihvatljivi rizici za `ivotnu sredinu i zdravlje ljudi i iii) neke od eti~kih dilemma
koje su tako|e proiza{le kao i vezani rizici i dobiti za ~oveka i `ivotnu sredinu }e biti
razmatrane. Moralne i eti~ke zabrintosti su va`ni faktori koji uti~u na publiku koja
ima averziju prema GMO i bi}e navedeni neki karakteristi~ni slu~ajevi.
GENETICALY MODIFIED ORGANISMS (GMO) - RISKS
AND BENEFITS IN HUMAN POPULATION FEEDING
Developing transgenic crops has become routine within the last two decades and has
changed the nature of plant breeding substantially.The potential for transgenic crops
to alleviate human hunger and the possible effects on human health are discussed.
Feeding a human population of ten billion in the foreseeable future represent the ethical justification for employing such biotechnology. Risks and benefits to the food security and environment resulting from genetic engineering of crops for resistance to
biotic and abiotic stresses are considered, in addition to effects on biodiversity. Dilemmas, that have arisen as a result of the development and deployment of transgenic
crop plants such as: i) do transgenic crops represent the solution to world hunger; ii)
unacceptable risks to the environment and human health and; iii) some of the ethical
dilemmas that have also arisen will be discussed as well as associated risk and benefits
to man and the environment.The moral and ethical concerns are important factors in
influencing a risk-averse public and several characteristic cases will be presented.
156
LISTA AUTORA / AUTOR INDEX
A
A}in V.26, 39
Ad`i} S. 62, 70, 71, 73, 111
Aksi} M. 18, 25, 36
al-Sloge O. 123
Ambrus H. 6
Andjelkovi} V. 129
An|elkovi} B. 59, 99, 100
An|elkovi} S. 65, 99
An|elkovi} V. 10, 112, 127, 128
Atlagi} J. 46, 55
B
Babi} M. 32, 34, 129
Babi} S. 59, 66
Babi} V. 34, 129
Bale{evi}-Tubi} S. 42, 43
Banjac B. 17
Bara} G. 86, 122
Barnabás B. 6
Bekavac G. 22
Bekovi} D. 36
Berenji J. 8, 56, 116, 134
Berlekovi} D. 125
Bijeli} S. 132
Blagojevi} I. 134
Blagojevi} S. 133
Boca Z. 121
Bo}anski J. 11, 138, 140, 141
Bogdanovi} B. 132
Bokan N. 19
Boscaleri F. 107
Bo{kovi} J. 16, 24, 105
Bo{njakovi} D. 86, 122
Bo`inovi} S. 20, 35, 128, 147
Brankov M. 37
Brankovi} G. 30, 102, 123
Bratkovi} K. 28, 102
Brbakli} Lj. 109
Brdar-Jokanovi} M. 75
Brki} I. 104
Bulaji} A. 116
C
Cerovi} R. 90, 91
Cerovi} S. 132
Cindri} P. 95, 96
Crevar M. 35, 128, 147
Crnobarac J. 54
Cvetkovi} M. 59
Cviki} D. 62, 70, 71, 73, 75, 111, 130
Cvjetkovi} B. 79
^
^amd`ija Z. 23, 35, 38, 146, 147
^anak P. 46
^obanovi} L. 39
^oli} S. 85
^ukanovi} J. 86, 134
]
]ali} I. 67, 102, 123
]upina B. 58, 68
D
Damnjanovi} J. 72, 74
Dani~i} V. 79
Dedi} B. 47, 114, 142
Dedi} D. 11, 141
Deleti} N. 25, 36
Deli} N. 20, 34
Dimitrijevi} A. 113, 114
Dimitrijevi} M. 17, 120
Dini} B. 99
Djan M. 108
Djinovi} I. 61
Dodig D. 28, 30, 112
Dori} M. 154
Dragi~evi} V. 37
Dra`i} S. 133, 150
\
\alovi} I. 22
\inovi} N. 61
\okovi} V. 140
\or|evi} M. 90
\or|evi} M. 69, 72, 74, 111
\or|evi} O. 74
\or|evi} R. 69, 74
\or|evi} V. 42, 43, 58, 117
\uki} N. 16
\uki} V. 42, 43
\uri} V. 14, 15, 39
\uri} V. 25, 36
\urovi} D. 29
F
Filipovi} M. 23, 33, 34, 38, 146, 147
Filipovi} V. 52
Fladung M. 118
157
Forgi} G. 121
Fotiri}-Ak{i} M. 85, 92, 93, 94, 131
Fulgosi H. 104
G
Galiba G. 6
Galovi} V. 106, 118
Garalei} B. 14
Girek Z. 70, 73, 75, 130
Glamo~lija \. 49, 133
Gli{i} I. 84, 90
Golo{in B. 132
Grbi} J. 52
Gr~i} N. 35, 128, 147
Gud`i} S. 25
H
Hladni N. 45
Hristov N. 14, 15, 26, 39
I
Ignjatov M. 144
Ignjatovi}-Mici} D. 10, 20, 110, 112, 126,
128
Ikanovi} J. 49
Ili} Z. 98
Imerovski I. 113, 114
Injac M. 154
Isajev V. 9, 78, 79, 80, 82, 87, 88, 89
Ivan J. 124
Ivani{evi} D. 95, 96
Ivanovi} M. 22, 31
Iveti} V.80, 82, 83, 87, 89
J
Ja}imovi} G. 54
Jak{i} S. 49
Jambrovi} A. 104
Jankovi} S. 27
Je~menica M. 125
Jeli} M. 19
Jevremovi} D. 91
Jevti} G. 64, 66, 99
Jevti}-Mu~ibabi} R. 52
Joci} S. 45, 47, 113, 114
Jockovi} B. 14, 15, 26
Jockovi} \. 22, 31
Jockovi} M. 46, 149
Jokovi} R. 131
Jovanovi} @. 58
Jovi~i} D. 144
K
158
Kadar I. 21
Kandi} V. 28, 30, 102, 146
Karagi} \. 63, 67, 68, 143
Kati} S. 63, 68
Keserovi} Z. 154
Kla{nja B. 106
Kne`evi} D. 16, 18, 19, 21, 24, 29, 30, 53,
103
Kobiljski B. 7, 109
Komarnicki-]irli} A. 89
Kondi}-[pika A. 7, 15, 39, 109
Konstantinov K. 9, 156
Kora} N. 95, 96
Kostadinovi} M. 33, 110, 115
Kosti} M. 49
Kova~evi} D. 33, 48
Kova~evi} D. 152
Kova~evi} V. 18, 21, 104
Kraljevi}-Balali} M. 45, 134
Kravi} N. 126, 127
Kresovi} B. 37
Krgovi} S. 14
Krsmanovi} S. 124
Krsti} B. 116
Krsti} M. 80
Kudryavtsev M. A. 16, 103
Kuljan~i} I. 95, 96
L
Lali} N. 98
Latkovi} D. 54, 56
Lavadinovi} V. 82, 83, 88
Lazi} B. 152
Le~i} N. 149
Leden~an T. 104
Li~ina V. 85
Lon~ar G. 138
Lon~ari} Z. 21, 104
Lu~i} A. 78
Lugi} Z. 64, 65, 66, 99
Luki} M. 84, 91
Ljubojevi} M. 86, 122, 134
M
Madi} M. 18, 19, 29
Magazin N. 154
Male{evi} M. 49
Malid`a G. 113
Mandi} V. 27
Mari} S. 84
Marinkovi} B. 54
Marinkovi} R. 46, 55, 149
Marjanovi} Jeromela A. 46, 55, 144, 149
Markovi} J. 64
Markovi} S. 85
Markovi} @. 60
Martinovi} Z. 14
Marton L. C. 6
Mataruga M. 9, 79, 82
Mato{a M. 50
Matovi} B. 83
Medi} M. 95, 96
Medovi} A. 58
Mészáros A. 6
Mészáros K. 6
Mi}anovi} D. 24
Mihailovi} V. 68, 143
Mijatovi} M. 60, 69
Miki} A. 42, 43, 51, 58, 63, 68, 117, 143
Mikli~ V. 45, 47, 113, 114, 142
Miladinovi} D. 113, 114
Miladinovi} J. 42, 43
Milanovi} B. 61
Mila{inovi} M. 155
Mila{inovi} [ereme{i} M. 148
Milenkovi} J. 59
Milenkovi} S. 91
Mili} B. 154
Mili} D. 63, 143
Milivojevi} J. 94
Milo{evi} Bo. 98
Milo{evi} Br. 63, 67, 68, 143
Milo{evi} D. 144
Milo{evi} M. 139, 153
Milo{evi} N. 90
Milovac @. 55
Milovanovi} J. 107, 135
Miljkovi} D. 81
Mitrovi} B. 22, 31
Mitrovi} M. 91
Mitrovi} P. 55
Mladenov N. 14, 15, 26, 39
Mladenovi} Drini} S. 9, 10, 12, 23, 33,
38, 48, 51, 110, 115, 126, 146, 155, 156
Mladenovi} E. 122, 134
Mladenovi} M. 100
Mratini} E. 131
Mr|a J. 47, 142
N
Nastasi} A. 31
Németh-Kisgyörgy B. 6
Nikoli} A. 80, 87, 112, 128
Nikoli} D. 85, 92, 93, 94
Nikoli} M. 94
Nikoli} Z. 117, 144
Nini} Todorovi} J. 132
Ni{avi} A. 37
Noni} M. 107, 135
O
Obreht D. 108
Ognjanov M. 86
Ognjanov V. 86, 122, 134
Ordon F. 102
Orlovi} S. 9, 81, 106, 118
P
Paji} Z. 148
Pani} Grba B. 124
Pap P. 106
Papri} \. 96
Paunovi} A.18, 19, 29
Paunovi} S. 91
Pavlov J. 35, 146, 147
Pavlovi} N. 60, 62, 70, 73, 111, 130
Pavlovi} R. 60, 130
Pavlovi} S. 62, 70, 71, 75, 130
Peri} V. 48, 51
Perovi} D. 102
Pe{eva V. 100
Petrovi} K. 42, 43
Petrovi} S. 44, 53
Petrovi} S. 17, 120
Pilipovi} A. 81
Popov M. 95
Popovi} V. 49
Popovi} V. 83, 88
Predojevi} M. 86, 122
Priji} @. 105
Prodanovi} S. 27, 70, 73, 129
Purar B. 22
R
Radi~evi} S. 84, 90, 91
Radi} V. 46, 55, 149
Radivojevi} S. 52
Radoj~i} A. 33
Radosavljevi} M. 23, 148
Radovi} A. 93, 94
Radovi} J. 64, 65, 66
Rakonjac Lj. 78
Rakonjac V. 85, 92, 93, 94
Rakszegi M. 6
Ra{i} S. 100
Ristanovi} B. 98
Risti} D. 33, 110, 115, 116, 126
S
159
Sabado{ V. 121, 124, 141
Sági L. 6
Salem Ahsyee R. 67, 123
Samard`i} S. 98
Savi} Ivanov M. 125
Se~anski M. 32, 37
Sekuli} O. 121
Seme~enko V. 23
Sikora V. 8, 56
Simi} M. 37, 38, 115
Smikal P. 58
Sokolovi} D. 59, 65, 66
Soltész A. 6
Sori} R. 104
Spasi} Z. 98
Spasojevi} I. 37
Spitkó T. 6
Srdi} J. 32, 148
Srebri} M. 48, 51, 155
Stan~i} I. 30, 44, 53
Stanisavljevi} D. 31
Stankovi} G. 110, 146
Stankovi} I. 116
Stevanovi} M. 23, 28, 35, 38, 146, 147
Stipe{evi} B. 23, 38
Stojakovi} M. 22, 31
Stojkovi} S. 25, 36
Stojni} S. 81
Sudari} A. 12, 50
Szira F. 6
[
[ija~i}-Nikoli} M. 81, 83, 107, 135
[imi} D. 12, 21, 104
[kori} D. 45
[trbanovi} R. 64, 65, 66
[urlan-Momirovi} G. 28, 30, 32, 34, 62,
64, 67, 102, 123
T
Tan~i} S. 47
Terzi} S. 47, 55
Todorovi} G. 32, 72
Tomi} G. 138
Tomi} Z. 59
Torbica A. 117
160
Trajkovi} J. 93
Treski} S. 109
Trkulja D. 109
Trudi} B. 106
U
Ugrinovi} M.75
V
Vágújfalvi A. 6
Van~etovi} J. 10, 20, 126, 128, 129
Vapa Lj. 108
Vasi} T. 64, 65
Vasiljevi} S. 63, 67, 68, 123, 143
Veli~kovi} N. 108
Vettori C. 107, 118
Vidi} M. 49
Viloti} D. 81
Vratari} M. 50
Vu~urovi} A. 116
Vujo{evi} A. 72
Vujovi} J. 138
Vuleti} M. 127
Y
Yurievna Dragovi} A. 16, 103
Z
Zdjelar G. 144
Zdravkovi} J. 60, 62, 69, 70, 71, 73, 75,
111, 130
Zdravkovi} M. 60, 62, 73, 75, 111, 130
Zduni} Z. 104
Zec G. 92
Ze~evi} B. 69, 72, 74
Ze~evi} V. 16, 24, 105
Zlatkovi} B. 58
Zori} M. 28, 102, 106
@
@ili} S. 155
@ivanovi} T. 32, 60, 67
@ivi} J. 30, 44, 53
@ivkovi} B. 59
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