RP-HPLC Determination of Zosteric Acid in
Zostera noltii from Turkish Coastlines
Levent Cavasa,b, Gamze Topcamb, Hakan Alyuruk
Dokuz Eylül University, Faculty of Science, Department of Chemistry, Division of Biochemistry,
Tinaztepe Campus, İzmir, [email protected]
Dokuz Eylül University, Graduate School of Natural and Applied Sciences, Department of
Chemistry, Tinaztepe Campus, İzmir
Objectives: Zostera noltii is a widely distributed sea grass in the Mediterranean Sea. Its dead
leaves are accumulated like other sea grasses on the Aegean coastlines of Turkey. In addition to
accumulation, a tide event was observed in the Zostera noltii meadows during winter season in
Urla-İzmir, Turkey. Zosteric acid is an important secondary metabolite of Zostera spp against
fouling organisms. Determination of zosteric acid contents in Zostera noltii by using HPLC was
investigated in this research.
Methods: The samples were collected from Urla, İzmir and dried at room temperature. Dried
samples were powdered and then extracted with methanol. Detection of zosteric acid was performed
by RP-HPLC system mounted with UV detector at 280 nm and 360 nm. Peak area of the analyte
was measured by calibration curve of standard p-coumaric acid. Analytes were eluted at 1 mL/min
flow rate with gradient solvent system consisted of 0.1% TFA in water and methanol.
Results: The mean retention time for standard p-coumaric acid and zosteric acid were found as
26.8 ± 0.5 and 20.5 ± 0.8 min, respectively. R2 value for calibration curve of p-coumaric acid was
0.9955. The mean retention time for zosteric acid in methanol extract of Z. noltii was found as 21.4
± 0.1 min. Mean zosteric acid content in methanol extracts of Z. noltii was found as 784.3 ± 13.6
µg zosteric acid/g DW.
Discussion & Conclusion: Since zosteric acid has a commercial importance for antifouling paint
industry, Zostera noltii samples from areas where tide events observed could be collected and
evaluated as an eco-friendly ingredient of antifouling paints.
Keywords: antifouling, HPLC, secondary metabolite, Zostera noltii, zosteric acid.
Acknowledgements: The authors thank The Scientific and Technological Research Council of
Turkey (TÜBİTAK) for financial support of the study (Grant no: 111T166).
21. Ulusal Biyoloji Kongresi, 03–07 Eylül 2012, Ege Üniversitesi, İzmir, Türkiye

SA–001 RP-HPLC Determination of Zosteric Acid in Zostera noltii